Restriction map for
pFN21AE2030
(Circular) (Six-base) MAPSORT of: pFN21AE2030.seq Check: 3387 from: 1 to: 6565
pFN21AE2030 6565 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4375 278
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2487 3908 1937
Size: 550 1421 4594
Fragments arranged by size:
4594 1421 550
Acc65I G'GTAC_C
Cuts at: 3886 3886
Size: 6565
AclI AA'CG_TT
Cuts at: 1563 4692 5065 1563
Size: 3129 373 3063
Fragments arranged by size:
3129 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3160 3615 4628 6012 6280 1632
Size: 1528 455 1013 1384 268 1917
Fragments arranged by size:
1917 1528 1384 1013 455 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6368
AflIII A'CryG_T
Cuts at: 1203 5464 6441 1203
Size: 4261 977 1327
Fragments arranged by size:
4261 1327 977
AgeI A'CCGG_T
Cuts at: 5396 5396
Size: 6565
AhdI GACnn_n'nnGTC
Cuts at: 5294 5294
Size: 6565
AleI CACnn'nnGTG
Cuts at: 1166 3688 1166
Size: 2522 4043
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2254 1184
Size: 1070 5495
AlwNI CAG_nnn'CTG
Cuts at: 2364 3418 4308 5880 2364
Size: 1054 890 1572 3049
Fragments arranged by size:
3049 1572 1054 890
ApaLI G'TGCA_C
Cuts at: 4622 5778 4622
Size: 1156 5409
ApoI r'AATT_y
Cuts at: 1493 3704 3874 4100 4252 6361 1493
Size: 2211 170 226 152 2109 1697
Fragments arranged by size:
2211 2109 1697 226 170 152
AseI AT'TA_AT
Cuts at: 160 1032 5119 160
Size: 872 4087 1606
Fragments arranged by size:
4087 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
AvaI C'yCGr_G
Cuts at: 1870 1943 3890 1870
Size: 73 1947 4545
Fragments arranged by size:
4545 1947 73
BamHI G'GATC_C
Cuts at: 3895 4262 3895
Size: 367 6198
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2238 3886 5341 618
Size: 325 217 627 451 1648 1455 1842
Fragments arranged by size:
1842 1648 1455 627 451 325 217
BanII G_rGCy'C
Cuts at: 1391 2298 3884 1391
Size: 907 1586 4072
Fragments arranged by size:
4072 1586 907
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2373 2396 3210 3668 928
Size: 971 474 23 814 458 3825
Fragments arranged by size:
3825 971 814 474 458 23
BbvCI CC'TCA_GC
Cuts at: 3390 3390
Size: 6565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4756 4790 1903
Size: 34 2819 34 3678
Fragments arranged by size:
3678 2819 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4457 5667 4457
Size: 1210 5355
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6565
BfrBI ATG'CAT
Cuts at: 3220 3220
Size: 6565
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5176 136
Size: 107 122 71 631 605 3504 1525
Fragments arranged by size:
3504 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6560 6560
Size: 6565
BlpI GC'TnA_GC
Cuts at: 3982 3982
Size: 6565
Bme1580I G_kGCm'C
Cuts at: 1790 4626 5782 1790
Size: 2836 1156 2573
Fragments arranged by size:
2836 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6565
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2374 4296 5254
Size: 416 53 83 399 24 957 1922 958
Cuts at: 5254 442
Size: 1753
Fragments arranged by size:
1922 1753 958 957 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6565
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2341 3756 5225 1239
Size: 1102 1415 1469 2579
Fragments arranged by size:
2579 1469 1415 1102
Bpu10I CC'TnA_GC
Cuts at: 2573 3390 2573
Size: 817 5748
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3011 3745 4690 5555 5853 3011
Size: 734 945 865 298 3723
Fragments arranged by size:
3723 945 865 734 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5228 882
Size: 617 3729 2219
Fragments arranged by size:
3729 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4356 493
Size: 711 3152 2702
Fragments arranged by size:
3152 2702 711
BsaBI GATnn'nnATC
Cuts at: 2886 6559 2886
Size: 3673 2892
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4372 4754 5389 275
Size: 53 83 186 936 2839 382 635 1451
Fragments arranged by size:
2839 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3199 4998 5396 5670 5817 6228 3199
Size: 1799 398 274 147 411 3536
Fragments arranged by size:
3536 1799 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6340
Fragments arranged by size:
6340 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6565
BseYI C'CCAG_C
Cuts at: 5768 6264 5768
Size: 496 6069
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5992
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4015 4776 5804 1540
Size: 275 186 2014 761 1028 2301
Fragments arranged by size:
2301 2014 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2071 2262 3054 4626 4711 5782 2071
Size: 191 792 1572 85 1071 2854
Fragments arranged by size:
2854 1572 1071 792 191 85
BsmI GAATG_Cn'
Cuts at: 4076 4169 4076
Size: 93 6472
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2262 2298 3054 3884 4626 4711
Size: 399 281 191 36 756 830 742 85
Cuts at: 4711 5782 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 830 756 742 399 281 191
85 36
BspEI T'CCGG_A
Cuts at: 6228 6228
Size: 6565
BspHI T'CATG_A
Cuts at: 4454 6184 4454
Size: 1730 4835
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3016 3920 844
Size: 2172 904 3489
Fragments arranged by size:
3489 2172 904
BsrBI CCG'CTC
Cuts at: 1394 1823 2017 4452 1394
Size: 429 194 2435 3507
Fragments arranged by size:
3507 2435 429 194
BsrDI GCAATG_nn'
Cuts at: 65 1248 3192 3565 5060 5234 65
Size: 1183 1944 373 1495 174 1396
Fragments arranged by size:
1944 1495 1396 1183 373 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5209 5396 1700
Size: 110 3399 187 2869
Fragments arranged by size:
3399 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2812 3579 96
Size: 2716 767 3082
Fragments arranged by size:
3082 2716 767
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6565
BssSI C'ACGA_G
Cuts at: 2103 4625 5637 2103
Size: 2522 1012 3031
Fragments arranged by size:
3031 2522 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6565
BstBI TT'CG_AA
Cuts at: 5369 5369
Size: 6565
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6565
BstYI r'GATC_y
Cuts at: 1972 2350 2785 3895 4262 4647 4664 6116 6202
Size: 378 435 1110 367 385 17 1452 86
Cuts at: 6202 6214 6560 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1110 435 385 378 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3689 513
Size: 552 2624 3389
Fragments arranged by size:
3389 2624 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3077 487
Size: 683 56 1851 3975
Fragments arranged by size:
3975 1851 683 56
BtsI GCAGTG_nn'
Cuts at: 2922 4081 4875 4895 2922
Size: 1159 794 20 4592
Fragments arranged by size:
4592 1159 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6565
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2971 3006 529
Size: 35 2407 35 4088
Fragments arranged by size:
4088 2407 35 35
DraI TTT'AAA
Cuts at: 2746 3869 2746
Size: 1123 5442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3847 5572 809
Size: 507 2531 1725 1802
Fragments arranged by size:
2531 1802 1725 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4012 4901 6530
Size: 54 1425 50 275 18 2182 889 1629
Cuts at: 6530 8
Size: 43
Fragments arranged by size:
2182 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4012 1537
Size: 275 2200 4090
Fragments arranged by size:
4090 2200 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2286 4495 1353
Size: 933 2209 3423
Fragments arranged by size:
3423 2209 933
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5152 5526 5672 387
Size: 1064 138 3563 374 146 1280
Fragments arranged by size:
3563 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2341 3160 3615 3756 4628 5225 6012
Size: 393 709 819 455 141 872 597 787
Cuts at: 6012 6280 1239
Size: 268 1524
Fragments arranged by size:
1524 872 819 787 709 597 455 393
268 141
EcoO109I rG'GnC_Cy
Cuts at: 2269 2269
Size: 6565
EcoRI G'AATT_C
Cuts at: 3874 4252 3874
Size: 378 6187
EcoRV GAT'ATC
Cuts at: 2969 2969
Size: 6565
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2573 2573
Size: 6565
FspI TGC'GCA
Cuts at: 5071 5071
Size: 6565
HaeII r_GCGC'y
Cuts at: 1678 5712 1678
Size: 4034 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5206 5238 5280 5312 1373
Size: 32 135 32 3634 32 42 32 2626
Fragments arranged by size:
3634 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3328 3909 4155 669
Size: 988 281 1390 581 246 3079
Fragments arranged by size:
3079 1390 988 581 281 246
HindIII A'AGCT_T
Cuts at: 748 2469 2477 2759 3597 748
Size: 1721 8 282 838 3716
Fragments arranged by size:
3716 1721 838 282 8
HpaI GTT'AAC
Cuts at: 4155 4155
Size: 6565
KpnI G_GTAC'C
Cuts at: 3890 3890
Size: 6565
MfeI C'AATT_G
Cuts at: 3306 4164 3306
Size: 858 5707
MluI A'CGCG_T
Cuts at: 6441 6441
Size: 6565
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5679 5863 1305
Size: 4374 184 2007
Fragments arranged by size:
4374 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4743
Fragments arranged by size:
4743 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2949 3575 3688 4523 4882 5041 518
Size: 648 1783 626 113 835 359 159 2042
Fragments arranged by size:
2042 1783 835 648 626 359 159 113
MspA1I CmG'CkG
Cuts at: 1606 3091 3981 4658 5806 6051 1606
Size: 1485 890 677 1148 245 2120
Fragments arranged by size:
2120 1485 1148 890 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6013
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6565
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6565
NotI GC'GGCC_GC
Cuts at: 4012 4012
Size: 6565
NsiI A_TGCA'T
Cuts at: 3222 3222
Size: 6565
NspI r_CATG'y
Cuts at: 1132 1229 2220 2530 2960 3365 3923 5468 1132
Size: 97 991 310 430 405 558 1545 2229
Fragments arranged by size:
2229 1545 991 558 430 405 310 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6565
PciI A'CATG_T
Cuts at: 5464 5464
Size: 6565
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2364 3064 1108
Size: 1256 700 4609
Fragments arranged by size:
4609 1256 700
PmeI GTTT'AAAC
Cuts at: 3869 3869
Size: 6565
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 6147 6179 4634
Size: 32 1481 32 5020
Fragments arranged by size:
5020 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6565
PsiI TTA'TAA
Cuts at: 2733 4135 2733
Size: 1402 5163
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2434 2466 2434
Size: 32 6533
PstI C_TGCA'G
Cuts at: 830 3371 3730 3917 830
Size: 2541 359 187 3478
Fragments arranged by size:
3478 2541 359 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6565
PvuII CAG'CTG
Cuts at: 3091 3091
Size: 6565
SalI G'TCGA_C
Cuts at: 1936 3907 1936
Size: 1971 4594
SapI GCTCTTCn'nnn_
Cuts at: 2286 2286
Size: 6565
SbfI CC_TGCA'GG
Cuts at: 3917 3917
Size: 6565
ScaI AGT'ACT
Cuts at: 4813 6525 4813
Size: 1712 4853
SfcI C'TryA_G
Cuts at: 826 1045 2455 2673 2769 3367 3726 3913 5048
Size: 219 1410 218 96 598 359 187 1135
Cuts at: 5048 5729 5920 826
Size: 681 191 1471
Fragments arranged by size:
1471 1410 1135 681 598 359 219 218
191 187 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6565
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6565
SmaI CCC'GGG
Cuts at: 1872 3892 1872
Size: 2020 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2990 3760 4669 5570 5832 820
Size: 197 926 1047 770 909 901 262 1553
Fragments arranged by size:
1553 1047 926 909 901 770 262 197
SnaBI TAC'GTA
Cuts at: 493 4356 493
Size: 3863 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6565
SphI G_CATG'C
Cuts at: 1132 3923 1132
Size: 2791 3774
SspI AAT'ATT
Cuts at: 5 52 4489 5
Size: 47 4437 2081
Fragments arranged by size:
4437 2081 47
StuI AGG'CCT
Cuts at: 1517 3395 1517
Size: 1878 4687
StyI C'CwwG_G
Cuts at: 513 1065 3396 3520 3789 4004 513
Size: 552 2331 124 269 215 3074
Fragments arranged by size:
3074 2331 552 269 215 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2175 2930 4592 4609 4762 4947 5392
Size: 1255 595 755 1662 17 153 185 445
Cuts at: 5392 325
Size: 1498
Fragments arranged by size:
1662 1498 1255 755 595 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2812 3579 4811
Size: 274 80 33 51 1443 835 767 1232
Cuts at: 4811 6523 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1232 835 767 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6525 6557 6525
Size: 32 6533
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6565
XbaI T'CTAG_A
Cuts at: 2431 3901 2431
Size: 1470 5095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3518 3787 3518
Size: 269 6296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6565
XmaI C'CCGG_G
Cuts at: 1870 3890 1870
Size: 2020 4545
XmnI GAAnn'nnTTC
Cuts at: 4694 4694
Size: 6565
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4373 276
Size: 53 83 186 936 2839 2468
Fragments arranged by size:
2839 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI