Restriction map for
pFN21AE2028
(Circular) (Six-base) MAPSORT of: pFN21AE2028.seq Check: 1226 from: 1 to: 6538
pFN21AE2028 6538 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2076 2082 2674 2076
Size: 6 592 5940
Fragments arranged by size:
5940 592 6
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4348 278
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2088 3881 1937
Size: 151 1793 4594
Fragments arranged by size:
4594 1793 151
Acc65I G'GTAC_C
Cuts at: 3859 3859
Size: 6538
AclI AA'CG_TT
Cuts at: 1563 4665 5038 1563
Size: 3102 373 3063
Fragments arranged by size:
3102 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3129 3500 4601 5985 6253 1632
Size: 1497 371 1101 1384 268 1917
Fragments arranged by size:
1917 1497 1384 1101 371 268
AflII C'TTAA_G
Cuts at: 820 1017 3418 820
Size: 197 2401 3940
Fragments arranged by size:
3940 2401 197
AflIII A'CryG_T
Cuts at: 1203 5437 6414 1203
Size: 4234 977 1327
Fragments arranged by size:
4234 1327 977
AgeI A'CCGG_T
Cuts at: 5369 5369
Size: 6538
AhdI GACnn_n'nnGTC
Cuts at: 2216 5267 2216
Size: 3051 3487
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6538
AlwNI CAG_nnn'CTG
Cuts at: 4281 5853 4281
Size: 1572 4966
ApaLI G'TGCA_C
Cuts at: 4595 5751 4595
Size: 1156 5382
ApoI r'AATT_y
Cuts at: 1493 2316 2576 3058 3457 3847 4073 4225 6334
Size: 823 260 482 399 390 226 152 2109
Cuts at: 6334 1493
Size: 1697
Fragments arranged by size:
2109 1697 823 482 399 390 260 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5092 160
Size: 872 4060 1606
Fragments arranged by size:
4060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 2009 3863 1870
Size: 73 66 1854 4545
Fragments arranged by size:
4545 1854 73 66
AvrII C'CTAG_G
Cuts at: 3217 3217
Size: 6538
BamHI G'GATC_C
Cuts at: 3868 4235 3868
Size: 367 6171
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3535 3859 5314 618
Size: 325 217 627 1748 324 1455 1842
Fragments arranged by size:
1842 1748 1455 627 325 324 217
BanII G_rGCy'C
Cuts at: 1391 3857 1391
Size: 2466 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 928
Size: 971 660 4907
Fragments arranged by size:
4907 971 660
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4729 4763 1903
Size: 34 50 34 2708 34 3678
Fragments arranged by size:
3678 2708 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2014 2164 4430 5640 2014
Size: 150 2266 1210 2912
Fragments arranged by size:
2912 2266 1210 150
BclI T'GATC_A
Cuts at: 1548 2202 3751 1548
Size: 654 1549 4335
Fragments arranged by size:
4335 1549 654
BfrBI ATG'CAT
Cuts at: 3793 3793
Size: 6538
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 5149 136
Size: 107 122 71 631 605 370 3107 1525
Fragments arranged by size:
3107 1525 631 605 370 122 107 71
BglII A'GATC_T
Cuts at: 2743 6533 2743
Size: 3790 2748
BlpI GC'TnA_GC
Cuts at: 3955 3955
Size: 6538
Bme1580I G_kGCm'C
Cuts at: 1790 3538 3555 4599 5755 1790
Size: 1748 17 1044 1156 2573
Fragments arranged by size:
2573 1748 1156 1044 17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6538
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4269 5227 442
Size: 416 53 83 399 24 2852 958 1753
Fragments arranged by size:
2852 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6538
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3555 3555
Size: 6538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 3512 5198 1239
Size: 1097 1176 1686 2579
Fragments arranged by size:
2579 1686 1176 1097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4663 5528 5826 4663
Size: 865 298 5375
Fragments arranged by size:
5375 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 5201 882
Size: 617 822 2880 2219
Fragments arranged by size:
2880 2219 822 617
BsaAI yAC'GTr
Cuts at: 493 1204 4329 493
Size: 711 3125 2702
Fragments arranged by size:
3125 2702 711
BsaBI GATnn'nnATC
Cuts at: 6532 6532
Size: 6538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4345 4727 5362 275
Size: 53 83 186 936 2812 382 635 1451
Fragments arranged by size:
2812 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4971 5369 5643 5790 6201 4971
Size: 398 274 147 411 5308
Fragments arranged by size:
5308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3510 3540 1178
Size: 30 165 30 2107 30 4176
Fragments arranged by size:
4176 2107 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6538
BseYI C'CCAG_C
Cuts at: 5741 6237 5741
Size: 496 6042
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 1163
Size: 573 318 5647
Fragments arranged by size:
5647 573 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3988 4749 5777 1540
Size: 275 186 1987 761 1028 2301
Fragments arranged by size:
2301 1987 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3780 4599 4684 5755 3780
Size: 819 85 1071 4563
Fragments arranged by size:
4563 1071 819 85
BsmI GAATG_Cn'
Cuts at: 3346 3570 3633 4049 4142 3346
Size: 224 63 416 93 5742
Fragments arranged by size:
5742 416 224 93 63
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3538 3555 3780 3857 4599 4684 5755
Size: 399 1748 17 225 77 742 85 1071
Cuts at: 5755 1391
Size: 2174
Fragments arranged by size:
2174 1748 1071 742 399 225 85 77
17
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 4427 6157 4427
Size: 1730 4808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2076 2082 2674 3893 844
Size: 1232 6 592 1219 3489
Fragments arranged by size:
3489 1232 1219 592 6
BsrBI CCG'CTC
Cuts at: 1394 1823 4425 1394
Size: 429 2602 3507
Fragments arranged by size:
3507 2602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2883 3813 5033 5207 65
Size: 1183 1635 930 1220 174 1396
Fragments arranged by size:
1635 1396 1220 1183 930 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5182 5369 1700
Size: 110 3372 187 2869
Fragments arranged by size:
3372 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3576 96
Size: 3480 3058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6538
BssSI C'ACGA_G
Cuts at: 4598 5610 4598
Size: 1012 5526
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6538
BstBI TT'CG_AA
Cuts at: 5342 5342
Size: 6538
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6538
BstXI CCAn_nnnn'nTGG
Cuts at: 3103 3103
Size: 6538
BstYI r'GATC_y
Cuts at: 1972 2743 3484 3724 3868 4235 4620 4637 6089
Size: 771 741 240 144 367 385 17 1452
Cuts at: 6089 6175 6187 6533 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 771 741 385 367 346 240
144 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5986
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5799
Fragments arranged by size:
5799 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 4054 4848 4868 2422
Size: 1632 794 20 4092
Fragments arranged by size:
4092 1632 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3626 3661 3795 3830 529
Size: 35 3062 35 134 35 3237
Fragments arranged by size:
3237 3062 134 35 35 35
DraI TTT'AAA
Cuts at: 3304 3456 3842 3304
Size: 152 386 6000
Fragments arranged by size:
6000 386 152
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2783 5545 809
Size: 507 1467 2762 1802
Fragments arranged by size:
2762 1802 1467 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3985 4874 6503
Size: 54 1425 50 275 18 2155 889 1629
Cuts at: 6503 8
Size: 43
Fragments arranged by size:
2155 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3985 1537
Size: 275 2173 4090
Fragments arranged by size:
4090 2173 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2280 3496 3546 4468 1353
Size: 927 1216 50 922 3423
Fragments arranged by size:
3423 1216 927 922 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5125 5499 5645 387
Size: 1064 138 3536 374 146 1280
Fragments arranged by size:
3536 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 3129 3500 3512 4601 5198 5985
Size: 393 704 793 371 12 1089 597 787
Cuts at: 5985 6253 1239
Size: 268 1524
Fragments arranged by size:
1524 1089 793 787 704 597 393 371
268 12
EcoO109I rG'GnC_Cy
Cuts at: 2179 2179
Size: 6538
EcoRI G'AATT_C
Cuts at: 3058 3847 4225 3058
Size: 789 378 5371
Fragments arranged by size:
5371 789 378
FspI TGC'GCA
Cuts at: 2156 5044 2156
Size: 2888 3650
HaeII r_GCGC'y
Cuts at: 1678 3671 5685 1678
Size: 1993 2014 2531
Fragments arranged by size:
2531 2014 1993
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2202 2234 2281
Size: 32 135 32 535 32 63 32 47
Cuts at: 2281 2313 2705 2737 2902 2934 5179 5211 5253
Size: 32 392 32 165 32 2245 32 42
Cuts at: 5253 5285 1373
Size: 32 2626
Fragments arranged by size:
2626 2245 535 392 165 135 63 47
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2089 3882 4128 669
Size: 988 281 151 1793 246 3079
Fragments arranged by size:
3079 1793 988 281 246 151
HindIII A'AGCT_T
Cuts at: 748 3231 748
Size: 2483 4055
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KpnI G_GTAC'C
Cuts at: 3863 3863
Size: 6538
MfeI C'AATT_G
Cuts at: 3500 4137 3500
Size: 637 5901
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2605 5652 5836 1305
Size: 1300 3047 184 2007
Fragments arranged by size:
3047 2007 1300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4716
Fragments arranged by size:
4716 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3027 4496 4855 5014 518
Size: 648 1861 1469 359 159 2042
Fragments arranged by size:
2042 1861 1469 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3954 4631 5779 6024 1606
Size: 2348 677 1148 245 2120
Fragments arranged by size:
2348 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5986
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6538
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NsiI A_TGCA'T
Cuts at: 3795 3795
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 3793 3896 5441 1132
Size: 97 2564 103 1545 2229
Fragments arranged by size:
2564 2229 1545 103 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6538
PciI A'CATG_T
Cuts at: 5437 5437
Size: 6538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6538
PfoI T'CCnGG_A
Cuts at: 3358 3358
Size: 6538
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 6120 6152 4607
Size: 32 1481 32 4993
Fragments arranged by size:
4993 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2179
Size: 6538
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6538
PsiI TTA'TAA
Cuts at: 4108 4108
Size: 6538
PstI C_TGCA'G
Cuts at: 830 2084 2429 2482 3890 830
Size: 1254 345 53 1408 3478
Fragments arranged by size:
3478 1408 1254 345 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
SalI G'TCGA_C
Cuts at: 1936 2087 3880 1936
Size: 151 1793 4594
Fragments arranged by size:
4594 1793 151
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 4786 6498 4786
Size: 1712 4826
SfcI C'TryA_G
Cuts at: 826 1045 2080 2425 2478 3053 3886 5021 5702
Size: 219 1035 345 53 575 833 1135 681
Cuts at: 5702 5893 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1035 833 681 575 345 219
191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 3865 1872
Size: 1993 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3418 4642 5543 5805 820
Size: 197 926 1475 1224 901 262 1553
Fragments arranged by size:
1553 1475 1224 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 3793 3896 1132
Size: 2661 103 3774
Fragments arranged by size:
3774 2661 103
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 3217 3977 513
Size: 552 2152 760 3074
Fragments arranged by size:
3074 2152 760 552
SwaI ATTT'AAAT
Cuts at: 3456 3456
Size: 6538
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2406 4565 4582 4735 4920 5365 325
Size: 1255 826 2159 17 153 185 445 1498
Fragments arranged by size:
2159 1498 1255 826 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2973 3576 4784
Size: 274 80 33 51 1443 996 603 1208
Cuts at: 4784 6496 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1208 996 603 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2784 2816 2955 2987 3510 3542 6498 6530 2784
Size: 32 139 32 523 32 2956 32 2792
Fragments arranged by size:
2956 2792 523 139 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6538
XbaI T'CTAG_A
Cuts at: 3874 3874
Size: 6538
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6538
XmaI C'CCGG_G
Cuts at: 1870 3863 1870
Size: 1993 4545
XmnI GAAnn'nnTTC
Cuts at: 3284 4667 3284
Size: 1383 5155
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4346 276
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI