Restriction map for
pFN21AE2027
(Circular) (Six-base) MAPSORT of: pFN21AE2027.seq Check: 9854 from: 1 to: 6535
pFN21AE2027 6535 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4345 278
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3878 1937
Size: 1941 4594
Acc65I G'GTAC_C
Cuts at: 3856 3856
Size: 6535
AclI AA'CG_TT
Cuts at: 1563 4662 5035 1563
Size: 3099 373 3063
Fragments arranged by size:
3099 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2494 2587 2840 3364 3588 4598 5982 6250
Size: 862 93 253 524 224 1010 1384 268
Cuts at: 6250 1632
Size: 1917
Fragments arranged by size:
1917 1384 1010 862 524 268 253 224
93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6338
AflIII A'CryG_T
Cuts at: 1203 2897 3275 3602 5434 6411 1203
Size: 1694 378 327 1832 977 1327
Fragments arranged by size:
1832 1694 1327 977 378 327
AgeI A'CCGG_T
Cuts at: 5366 5366
Size: 6535
AhdI GACnn_n'nnGTC
Cuts at: 5264 5264
Size: 6535
AleI CACnn'nnGTG
Cuts at: 1166 2039 1166
Size: 873 5662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6535
AlwNI CAG_nnn'CTG
Cuts at: 3783 4278 5850 3783
Size: 495 1572 4468
Fragments arranged by size:
4468 1572 495
ApaLI G'TGCA_C
Cuts at: 3103 3427 4592 5748 3103
Size: 324 1165 1156 3890
Fragments arranged by size:
3890 1165 1156 324
ApoI r'AATT_y
Cuts at: 1493 3353 3764 3844 4070 4222 6331 1493
Size: 1860 411 80 226 152 2109 1697
Fragments arranged by size:
2109 1860 1697 411 226 152 80
AseI AT'TA_AT
Cuts at: 160 1032 5089 160
Size: 872 4057 1606
Fragments arranged by size:
4057 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
AvaI C'yCGr_G
Cuts at: 1870 1943 3122 3860 1870
Size: 73 1179 738 4545
Fragments arranged by size:
4545 1179 738 73
BamHI G'GATC_C
Cuts at: 3221 3865 4232 3221
Size: 644 367 5524
Fragments arranged by size:
5524 644 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3856 5311 618
Size: 325 217 627 2069 1455 1842
Fragments arranged by size:
2069 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2299 2848 3534 3854 1391
Size: 908 549 686 320 4072
Fragments arranged by size:
4072 908 686 549 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3137 928
Size: 971 1238 4326
Fragments arranged by size:
4326 1238 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4726 4760 1903
Size: 34 2789 34 3678
Fragments arranged by size:
3678 2789 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4427 5637 4427
Size: 1210 5325
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6535
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5146 136
Size: 107 122 71 631 605 3474 1525
Fragments arranged by size:
3474 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2343 6530 2343
Size: 4187 2348
BlpI GC'TnA_GC
Cuts at: 3952 3952
Size: 6535
Bme1580I G_kGCm'C
Cuts at: 1790 3107 3431 4596 5752 1790
Size: 1317 324 1165 1156 2573
Fragments arranged by size:
2573 1317 1165 1156 324
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6535
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2302 2603 3077
Size: 416 53 83 399 24 885 301 474
Cuts at: 3077 4266 5224 442
Size: 1189 958 1753
Fragments arranged by size:
1753 1189 958 885 474 416 399 301
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6535
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3351 3351
Size: 6535
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2283 2706 2991 5195 1239
Size: 1044 423 285 2204 2579
Fragments arranged by size:
2579 2204 1044 423 285
Bpu10I CC'TnA_GC
Cuts at: 2562 2562
Size: 6535
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 3042 3443 4660 5525 5823 2504
Size: 538 401 1217 865 298 3216
Fragments arranged by size:
3216 1217 865 538 401 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2465 2657 5198 882
Size: 617 966 192 2541 2219
Fragments arranged by size:
2541 2219 966 617 192
BsaAI yAC'GTr
Cuts at: 493 1204 4326 493
Size: 711 3122 2702
Fragments arranged by size:
3122 2702 711
BsaBI GATnn'nnATC
Cuts at: 2222 2348 6529 2222
Size: 126 4181 2228
Fragments arranged by size:
4181 2228 126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4342 4724 5359 275
Size: 53 83 186 936 2809 382 635 1451
Fragments arranged by size:
2809 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4968 5366 5640 5787 6198 4968
Size: 398 274 147 411 5305
Fragments arranged by size:
5305 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2066 2096 2168 2198 3521
Size: 30 165 30 663 30 72 30 1323
Cuts at: 3521 3551 3690 3711 3720 3741 1178
Size: 30 139 21 9 21 3972
Fragments arranged by size:
3972 1323 663 165 139 72 30 30
30 30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3404 3719 1186
Size: 2218 315 4002
Fragments arranged by size:
4002 2218 315
BseYI C'CCAG_C
Cuts at: 5738 6234 5738
Size: 496 6039
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2413 2974 1163
Size: 573 677 561 4724
Fragments arranged by size:
4724 677 573 561
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3985 4746 5774 1540
Size: 275 186 1984 761 1028 2301
Fragments arranged by size:
2301 1984 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2701 3107 3337 3431 4596 4681 5752 2701
Size: 406 230 94 1165 85 1071 3484
Fragments arranged by size:
3484 1165 1071 406 230 94 85
BsmI GAATG_Cn'
Cuts at: 3053 4046 4139 3053
Size: 993 93 5449
Fragments arranged by size:
5449 993 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2299 2701 2848 3107 3337 3431 3534
Size: 399 509 402 147 259 230 94 103
Cuts at: 3534 3854 4596 4681 5752 1391
Size: 320 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 509 402 399 320 259
230 147 103 94 85
BspEI T'CCGG_A
Cuts at: 6198 6198
Size: 6535
BspHI T'CATG_A
Cuts at: 4424 6154 4424
Size: 1730 4805
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2655 3890 844
Size: 1811 1235 3489
Fragments arranged by size:
3489 1811 1235
BsrBI CCG'CTC
Cuts at: 1394 1823 4422 1394
Size: 429 2599 3507
Fragments arranged by size:
3507 2599 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2722 5030 5204 65
Size: 1183 1474 2308 174 1396
Fragments arranged by size:
2308 1474 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5179 5366 1700
Size: 110 3369 187 2869
Fragments arranged by size:
3369 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2543 2807 96
Size: 2447 264 3824
Fragments arranged by size:
3824 2447 264
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6535
BssSI C'ACGA_G
Cuts at: 4595 5607 4595
Size: 1012 5523
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3549 794
Size: 2755 3780
BstBI TT'CG_AA
Cuts at: 5339 5339
Size: 6535
BstEII G'GTnAC_C
Cuts at: 1184 2642 1184
Size: 1458 5077
BstXI CCAn_nnnn'nTGG
Cuts at: 2780 2780
Size: 6535
BstYI r'GATC_y
Cuts at: 1972 2343 3221 3865 4232 4617 4634 6086 6172
Size: 371 878 644 367 385 17 1452 86
Cuts at: 6172 6184 6530 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 878 644 385 371 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2085 2455 2792 513
Size: 552 1020 370 337 4256
Fragments arranged by size:
4256 1020 552 370 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3309 487
Size: 683 56 2083 3713
Fragments arranged by size:
3713 2083 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 2678 3071 3529 4051 4845 4865 2455
Size: 223 393 458 522 794 20 4125
Fragments arranged by size:
4125 794 522 458 393 223 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6535
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3047 3082 529
Size: 35 2483 35 3982
Fragments arranged by size:
3982 2483 35 35
DraI TTT'AAA
Cuts at: 3839 3839
Size: 6535
DraIII CAC_nnn'GTG
Cuts at: 3549 3549
Size: 6535
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5542 809
Size: 507 4226 1802
Fragments arranged by size:
4226 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2452 3982 4871
Size: 54 1425 50 275 18 622 1530 889
Cuts at: 4871 6500 8
Size: 1629 43
Fragments arranged by size:
1629 1530 1425 889 622 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3982 1537
Size: 275 2170 4090
Fragments arranged by size:
4090 2170 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2160 3447 4465 1353
Size: 807 1287 1018 3423
Fragments arranged by size:
3423 1287 1018 807
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2915 5122 5496 5642 387
Size: 1064 138 1326 2207 374 146 1280
Fragments arranged by size:
2207 1326 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2283 2494 2587 2706 2840 2991 3364
Size: 393 651 211 93 119 134 151 373
Cuts at: 3364 3588 4598 5195 5982 6250 1239
Size: 224 1010 597 787 268 1524
Fragments arranged by size:
1524 1010 787 651 597 393 373 268
224 211 151 134 119 93
EcoRI G'AATT_C
Cuts at: 3353 3844 4222 3353
Size: 491 378 5666
Fragments arranged by size:
5666 491 378
FspI TGC'GCA
Cuts at: 5041 5041
Size: 6535
HaeII r_GCGC'y
Cuts at: 1678 5682 1678
Size: 4004 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3416 3448 3564 3596 3690
Size: 32 135 32 1844 32 116 32 94
Cuts at: 3690 3722 5176 5208 5250 5282 1373
Size: 32 1454 32 42 32 2626
Fragments arranged by size:
2626 1844 1454 135 116 94 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2236 3879 4125 669
Size: 988 281 298 1643 246 3079
Fragments arranged by size:
3079 1643 988 298 281 246
HindIII A'AGCT_T
Cuts at: 748 2405 2785 3093 748
Size: 1657 380 308 4190
Fragments arranged by size:
4190 1657 380 308
HpaI GTT'AAC
Cuts at: 4125 4125
Size: 6535
KpnI G_GTAC'C
Cuts at: 3860 3860
Size: 6535
MfeI C'AATT_G
Cuts at: 4134 4134
Size: 6535
MluI A'CGCG_T
Cuts at: 6411 6411
Size: 6535
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3141 3687 5649 5833 1305
Size: 1836 546 1962 184 2007
Fragments arranged by size:
2007 1962 1836 546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2454 10
Size: 54 1425 343 622 4091
Fragments arranged by size:
4091 1425 622 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 4493 4852 5011 518
Size: 648 873 2454 359 159 2042
Fragments arranged by size:
2454 2042 873 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2426 2631 3183 3384 3783 3951 4628 5776
Size: 820 205 552 201 399 168 677 1148
Cuts at: 5776 6021 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 820 677 552 399 245 205
201 168
NcoI C'CATG_G
Cuts at: 513 1065 2792 513
Size: 552 1727 4256
Fragments arranged by size:
4256 1727 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6535
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6535
NotI GC'GGCC_GC
Cuts at: 3982 3982
Size: 6535
NspI r_CATG'y
Cuts at: 1132 1229 2377 2916 3279 3606 3893 5438 1132
Size: 97 1148 539 363 327 287 1545 2229
Fragments arranged by size:
2229 1545 1148 539 363 327 287 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6535
PciI A'CATG_T
Cuts at: 3275 3602 5434 3275
Size: 327 1832 4376
Fragments arranged by size:
4376 1832 327
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2292 2753 1108
Size: 1184 461 4890
Fragments arranged by size:
4890 1184 461
PfoI T'CCnGG_A
Cuts at: 3499 3499
Size: 6535
PmeI GTTT'AAAC
Cuts at: 3839 3839
Size: 6535
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4604 4636 6117 6149 4604
Size: 32 1481 32 4990
Fragments arranged by size:
4990 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3622 1613
Size: 2009 4526
PsiI TTA'TAA
Cuts at: 4105 4105
Size: 6535
PstI C_TGCA'G
Cuts at: 830 2450 3524 3595 3887 830
Size: 1620 1074 71 292 3478
Fragments arranged by size:
3478 1620 1074 292 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6535
PvuII CAG'CTG
Cuts at: 2426 2631 3183 3384 3783 2426
Size: 205 552 201 399 5178
Fragments arranged by size:
5178 552 399 205 201
SalI G'TCGA_C
Cuts at: 1936 3877 1936
Size: 1941 4594
SbfI CC_TGCA'GG
Cuts at: 3887 3887
Size: 6535
ScaI AGT'ACT
Cuts at: 4783 6495 4783
Size: 1712 4823
SexAI A'CCwGG_T
Cuts at: 2142 2142
Size: 6535
SfcI C'TryA_G
Cuts at: 826 1045 2446 3520 3591 3883 5018 5699 5890
Size: 219 1401 1074 71 292 1135 681 191
Cuts at: 5890 826
Size: 1471
Fragments arranged by size:
1471 1401 1135 1074 681 292 219 191
71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6535
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6535
SmaI CCC'GGG
Cuts at: 1872 3862 1872
Size: 1990 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2519 3057 3422 4639 5540 5802
Size: 197 926 576 538 365 1217 901 262
Cuts at: 5802 820
Size: 1553
Fragments arranged by size:
1553 1217 926 901 576 538 365 262
197
SnaBI TAC'GTA
Cuts at: 493 4326 493
Size: 3833 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6535
SphI G_CATG'C
Cuts at: 1132 3893 1132
Size: 2761 3774
SspI AAT'ATT
Cuts at: 5 52 4459 5
Size: 47 4407 2081
Fragments arranged by size:
4407 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6535
StyI C'CwwG_G
Cuts at: 513 1065 2792 3672 3974 513
Size: 552 1727 880 302 3074
Fragments arranged by size:
3074 1727 880 552 302
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2604 4562 4579 4732 4917 5362 325
Size: 1255 1024 1958 17 153 185 445 1498
Fragments arranged by size:
1958 1498 1255 1024 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2543 2807 4781
Size: 274 80 33 51 1443 566 264 1974
Cuts at: 4781 6493 96
Size: 1712 138
Fragments arranged by size:
1974 1712 1443 566 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3063 3095 3711 3743 6495 6527 3063
Size: 32 616 32 2752 32 3071
Fragments arranged by size:
3071 2752 616 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6535
XbaI T'CTAG_A
Cuts at: 3871 3871
Size: 6535
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2211 2758 2936 2966 3670 3729 2211
Size: 547 178 30 704 59 5017
Fragments arranged by size:
5017 704 547 178 59 30
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6535
XmaI C'CCGG_G
Cuts at: 1870 3860 1870
Size: 1990 4545
XmnI GAAnn'nnTTC
Cuts at: 3456 4664 3456
Size: 1208 5327
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4343 276
Size: 53 83 186 936 2809 2468
Fragments arranged by size:
2809 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI