Restriction map for
pFN21AE2025
(Circular) (Six-base) MAPSORT of: pFN21AE2025.seq Check: 3283 from: 1 to: 6529
pFN21AE2025 6529 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2743 2743
Size: 6529
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2880 4339 278
Size: 53 83 186 936 1344 1459 2468
Fragments arranged by size:
2468 1459 1344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3872 1937
Size: 1935 4594
Acc65I G'GTAC_C
Cuts at: 2943 3249 3850 2943
Size: 306 601 5622
Fragments arranged by size:
5622 601 306
AclI AA'CG_TT
Cuts at: 1563 4656 5029 1563
Size: 3093 373 3063
Fragments arranged by size:
3093 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2861 4592 5976 6244 1632
Size: 1229 1731 1384 268 1917
Fragments arranged by size:
1917 1731 1384 1229 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6332
AflIII A'CryG_T
Cuts at: 1203 2856 3314 3489 5428 6405 1203
Size: 1653 458 175 1939 977 1327
Fragments arranged by size:
1939 1653 1327 977 458 175
AgeI A'CCGG_T
Cuts at: 5360 5360
Size: 6529
AhdI GACnn_n'nnGTC
Cuts at: 5258 5258
Size: 6529
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6529
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6529
AlwNI CAG_nnn'CTG
Cuts at: 2170 4272 5844 2170
Size: 2102 1572 2855
Fragments arranged by size:
2855 2102 1572
ApaI G_GGCC'C
Cuts at: 2079 2079
Size: 6529
ApaLI G'TGCA_C
Cuts at: 4586 5742 4586
Size: 1156 5373
ApoI r'AATT_y
Cuts at: 1493 3128 3838 4064 4216 6325 1493
Size: 1635 710 226 152 2109 1697
Fragments arranged by size:
2109 1697 1635 710 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5083 160
Size: 872 4051 1606
Fragments arranged by size:
4051 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2177 2259 2719 2747 3854 1870
Size: 73 119 115 82 460 28 1107 4545
Fragments arranged by size:
4545 1107 460 119 115 82 73 28
BamHI G'GATC_C
Cuts at: 2592 3042 3859 4226 2592
Size: 450 817 367 4895
Fragments arranged by size:
4895 817 450 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2105 2513 2943 2987 3249
Size: 325 217 627 318 408 430 44 262
Cuts at: 3249 3261 3698 3850 5305 618
Size: 12 437 152 1455 1842
Fragments arranged by size:
1842 1455 627 437 430 408 325 318
262 217 152 44 12
BanII G_rGCy'C
Cuts at: 1391 2079 2778 3237 3662 3848 1391
Size: 688 699 459 425 186 4072
Fragments arranged by size:
4072 699 688 459 425 186
BbeI G_GCGC'C
Cuts at: 2109 3702 2109
Size: 1593 4936
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2427 928
Size: 971 528 5030
Fragments arranged by size:
5030 971 528
BbvCI CC'TCA_GC
Cuts at: 2670 3518 2670
Size: 848 5681
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4720 4754 1903
Size: 34 2783 34 3678
Fragments arranged by size:
3678 2783 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4421 5631 4421
Size: 1210 5319
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6529
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2283 5140 136
Size: 107 122 71 631 605 611 2857 1525
Fragments arranged by size:
2857 1525 631 611 605 122 107 71
BglII A'GATC_T
Cuts at: 6524 6524
Size: 6529
BlpI GC'TnA_GC
Cuts at: 3946 3946
Size: 6529
Bme1580I G_kGCm'C
Cuts at: 1790 2079 3440 3733 4590 5746 1790
Size: 289 1361 293 857 1156 2573
Fragments arranged by size:
2573 1361 1156 857 293 289
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6529
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4260 5218 442
Size: 416 53 83 399 24 2843 958 1753
Fragments arranged by size:
2843 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2420 2640 2831 3571 5189 1239
Size: 1181 220 191 740 1618 2579
Fragments arranged by size:
2579 1618 1181 740 220 191
Bpu10I CC'TnA_GC
Cuts at: 2670 3518 2670
Size: 848 5681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3583 4654 5519 5817 3583
Size: 1071 865 298 4295
Fragments arranged by size:
4295 1071 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5192 882
Size: 617 3693 2219
Fragments arranged by size:
3693 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2503 4320 493
Size: 711 1299 1817 2702
Fragments arranged by size:
2702 1817 1299 711
BsaBI GATnn'nnATC
Cuts at: 3116 6523 3116
Size: 3407 3122
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2106 2877 3699 3785
Size: 53 83 186 936 573 771 822 86
Cuts at: 3785 4336 4718 5353 275
Size: 551 382 635 1451
Fragments arranged by size:
1451 936 822 771 635 573 551 382
186 86 83 53
BsaWI w'CCGG_w
Cuts at: 3039 4962 5360 5634 5781 6192 3039
Size: 1923 398 274 147 411 3376
Fragments arranged by size:
3376 1923 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2723 2753 3632 3662 1178
Size: 30 165 30 1320 30 879 30 4045
Fragments arranged by size:
4045 1320 879 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2444 2747 1186
Size: 919 339 303 4968
Fragments arranged by size:
4968 919 339 303
BseYI C'CCAG_C
Cuts at: 2475 2661 2846 5732 6228 2475
Size: 186 185 2886 496 2776
Fragments arranged by size:
2886 2776 496 186 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2293 2465 1163
Size: 573 557 172 5227
Fragments arranged by size:
5227 573 557 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3552 3979 4740 5768 1540
Size: 275 186 1551 427 761 1028 2301
Fragments arranged by size:
2301 1551 1028 761 427 275 186
BsiHKAI G_wGCw'C
Cuts at: 2252 2626 3674 4590 4675 5746 2252
Size: 374 1048 916 85 1071 3035
Fragments arranged by size:
3035 1071 1048 916 374 85
BsmI GAATG_Cn'
Cuts at: 3142 4040 4133 3142
Size: 898 93 5538
Fragments arranged by size:
5538 898 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2252 2626 2778 3237 3440 3662
Size: 399 289 173 374 152 459 203 222
Cuts at: 3662 3674 3733 3848 4590 4675 5746 1391
Size: 12 59 115 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 374 289 222
203 173 152 115 85 59 12
BspEI T'CCGG_A
Cuts at: 3039 6192 3039
Size: 3153 3376
BspHI T'CATG_A
Cuts at: 4418 6148 4418
Size: 1730 4799
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2390 2743 3884 844
Size: 1546 353 1141 3489
Fragments arranged by size:
3489 1546 1141 353
BsrBI CCG'CTC
Cuts at: 1394 1823 2404 2422 4416 1394
Size: 429 581 18 1994 3507
Fragments arranged by size:
3507 1994 581 429 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 5024 5198 65
Size: 1183 3776 174 1396
Fragments arranged by size:
3776 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2189 3380 5173 5360 1700
Size: 110 379 1191 1793 187 2869
Fragments arranged by size:
2869 1793 1191 379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6529
BssHII G'CGCG_C
Cuts at: 1925 2185 2524 1925
Size: 260 339 5930
Fragments arranged by size:
5930 339 260
BssSI C'ACGA_G
Cuts at: 3724 4589 5601 3724
Size: 865 1012 4652
Fragments arranged by size:
4652 1012 865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2170 794
Size: 1376 5153
BstBI TT'CG_AA
Cuts at: 5333 5333
Size: 6529
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6529
BstXI CCAn_nnnn'nTGG
Cuts at: 2462 2656 3710 3781 2462
Size: 194 1054 71 5210
Fragments arranged by size:
5210 1054 194 71
BstYI r'GATC_y
Cuts at: 1972 2142 2592 3042 3406 3859 4226 4611 4628
Size: 170 450 450 364 453 367 385 17
Cuts at: 4628 6080 6166 6178 6524 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 453 450 450 385 367 364
346 170 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2655 2778 2959 2991 3157 3534
Size: 552 939 651 123 181 32 166 377
Cuts at: 3534 3759 3780 513
Size: 225 21 3262
Fragments arranged by size:
3262 939 651 552 377 225 181 166
123 32 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2194 487
Size: 683 56 968 4822
Fragments arranged by size:
4822 968 683 56
BtsI GCAGTG_nn'
Cuts at: 3574 3715 4045 4839 4859 3574
Size: 141 330 794 20 5244
Fragments arranged by size:
5244 794 330 141 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6529
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6494
DraI TTT'AAA
Cuts at: 3833 3833
Size: 6529
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2431 5536 809
Size: 507 1115 3105 1802
Fragments arranged by size:
3105 1802 1115 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3051 3531 3976
Size: 54 1425 50 275 18 1221 480 445
Cuts at: 3976 4865 6494 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1221 889 480 445 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3976 1537
Size: 275 2164 4090
Fragments arranged by size:
4090 2164 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 2798 4459 1353
Size: 1325 120 1661 3423
Fragments arranged by size:
3423 1661 1325 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5116 5490 5636 387
Size: 1064 138 3527 374 146 1280
Fragments arranged by size:
3527 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2640 2831 2861 3571 4592 5189
Size: 393 788 220 191 30 710 1021 597
Cuts at: 5189 5976 6244 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1021 788 787 710 597 393 268
220 191 30
EcoO109I rG'GnC_Cy
Cuts at: 2075 2076 3690 2075
Size: 1 1614 4914
Fragments arranged by size:
4914 1614 1
EcoRI G'AATT_C
Cuts at: 3838 4216 3838
Size: 378 6151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2953 2311
Size: 642 5887
FspI TGC'GCA
Cuts at: 5035 5035
Size: 6529
HaeII r_GCGC'y
Cuts at: 1678 2109 2171 3702 5676 1678
Size: 431 62 1531 1974 2531
Fragments arranged by size:
2531 1974 1531 431 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 3290 3322 3622
Size: 32 135 32 1151 32 535 32 300
Cuts at: 3622 3654 5170 5202 5244 5276 1373
Size: 32 1516 32 42 32 2626
Fragments arranged by size:
2626 1516 1151 535 300 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3745 3873 4119 669
Size: 988 281 1807 128 246 3079
Fragments arranged by size:
3079 1807 988 281 246 128
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6529
HpaI GTT'AAC
Cuts at: 4119 4119
Size: 6529
KasI G'GCGC_C
Cuts at: 2105 3698 2105
Size: 1593 4936
KpnI G_GTAC'C
Cuts at: 2947 3253 3854 2947
Size: 306 601 5622
Fragments arranged by size:
5622 601 306
MfeI C'AATT_G
Cuts at: 4128 4128
Size: 6529
MluI A'CGCG_T
Cuts at: 6405 6405
Size: 6529
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5643 5827 1305
Size: 4338 184 2007
Fragments arranged by size:
4338 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3053 10
Size: 54 1425 343 1221 3486
Fragments arranged by size:
3486 1425 1221 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2229 3779 4487 4846 5005 518
Size: 648 1063 1550 708 359 159 2042
Fragments arranged by size:
2042 1550 1063 708 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2149 2850 2993 3525 3945 4622 5770
Size: 406 137 701 143 532 420 677 1148
Cuts at: 5770 6015 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 701 677 532 420 406 245
143 137
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 6529
NarI GG'CG_CC
Cuts at: 2106 3699 2106
Size: 1593 4936
NcoI C'CATG_G
Cuts at: 513 1065 2004 2655 2778 2959 3759 3780 513
Size: 552 939 651 123 181 800 21 3262
Fragments arranged by size:
3262 939 800 651 552 181 123 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6529
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 6529
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6529
NotI GC'GGCC_GC
Cuts at: 3976 3976
Size: 6529
NspI r_CATG'y
Cuts at: 1132 1229 2791 2809 2860 3139 3318 3493 3887
Size: 97 1562 18 51 279 179 175 394
Cuts at: 3887 5432 1132
Size: 1545 2229
Fragments arranged by size:
2229 1562 1545 394 279 179 175 97
51 18
PasI CC'CwG_GG
Cuts at: 1348 2308 3703 1348
Size: 960 1395 4174
Fragments arranged by size:
4174 1395 960
PciI A'CATG_T
Cuts at: 2856 3314 3489 5428 2856
Size: 458 175 1939 3957
Fragments arranged by size:
3957 1939 458 175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 2940 2965 3163 1108
Size: 1290 542 25 198 4474
Fragments arranged by size:
4474 1290 542 198 25
PfoI T'CCnGG_A
Cuts at: 3402 3650 3402
Size: 248 6281
PmeI GTTT'AAAC
Cuts at: 3833 3833
Size: 6529
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4598 4630 6111 6143 4598
Size: 32 1481 32 4984
Fragments arranged by size:
4984 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3690 3690
Size: 6529
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6529
PsiI TTA'TAA
Cuts at: 4099 4099
Size: 6529
PspOMI G'GGCC_C
Cuts at: 2075 2075
Size: 6529
PspXI vC'TCGA_Gb
Cuts at: 2177 2177
Size: 6529
PstI C_TGCA'G
Cuts at: 830 3881 830
Size: 3051 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6529
RsrII CG'GwC_CG
Cuts at: 2701 2701
Size: 6529
SacII CC_GC'GG
Cuts at: 2994 2994
Size: 6529
SalI G'TCGA_C
Cuts at: 1936 3871 1936
Size: 1935 4594
SapI GCTCTTCn'nnn_
Cuts at: 2798 2798
Size: 6529
SbfI CC_TGCA'GG
Cuts at: 3881 3881
Size: 6529
ScaI AGT'ACT
Cuts at: 2450 4777 6489 2450
Size: 2327 1712 2490
Fragments arranged by size:
2490 2327 1712
SexAI A'CCwGG_T
Cuts at: 3252 3252
Size: 6529
SfcI C'TryA_G
Cuts at: 826 1045 2218 3877 5012 5693 5884 826
Size: 219 1173 1659 1135 681 191 1471
Fragments arranged by size:
1659 1471 1173 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2107 3700 2107
Size: 1593 4936
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6529
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6529
SmaI CCC'GGG
Cuts at: 1872 2064 2721 3856 1872
Size: 192 657 1135 4545
Fragments arranged by size:
4545 1135 657 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2177 3562 4633 5534 5796 820
Size: 197 926 234 1385 1071 901 262 1553
Fragments arranged by size:
1553 1385 1071 926 901 262 234 197
SnaBI TAC'GTA
Cuts at: 493 4320 493
Size: 3827 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6529
SphI G_CATG'C
Cuts at: 1132 2809 3887 1132
Size: 1677 1078 3774
Fragments arranged by size:
3774 1677 1078
SspI AAT'ATT
Cuts at: 5 52 4453 5
Size: 47 4401 2081
Fragments arranged by size:
4401 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6529
StyI C'CwwG_G
Cuts at: 513 1065 2004 2655 2778 2928 2959 3288 3685
Size: 552 939 651 123 150 31 329 397
Cuts at: 3685 3759 3780 3968 513
Size: 74 21 188 3074
Fragments arranged by size:
3074 939 651 552 397 329 188 150
123 74 31 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2359 3659 4556 4573 4726 4911 5356
Size: 1255 779 1300 897 17 153 185 445
Cuts at: 5356 325
Size: 1498
Fragments arranged by size:
1498 1300 1255 897 779 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2448 3066 4775
Size: 274 80 33 51 1443 471 618 1709
Cuts at: 4775 6487 96
Size: 1712 138
Fragments arranged by size:
1712 1709 1443 618 471 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3476 3508 3632 3664 6489 6521 3476
Size: 32 124 32 2825 32 3484
Fragments arranged by size:
3484 2825 124 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6529
XbaI T'CTAG_A
Cuts at: 3865 3865
Size: 6529
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3400 3710 3400
Size: 310 6219
XhoI C'TCGA_G
Cuts at: 1943 2177 1943
Size: 234 6295
XmaI C'CCGG_G
Cuts at: 1870 2062 2719 3854 1870
Size: 192 657 1135 4545
Fragments arranged by size:
4545 1135 657 192
XmnI GAAnn'nnTTC
Cuts at: 4658 4658
Size: 6529
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2878 4337 276
Size: 53 83 186 936 1344 1459 2468
Fragments arranged by size:
2468 1459 1344 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI
FspAI NruI NsiI PacI PmlI PsrI PvuII SacI
SanDI SfiI SrfI SwaI