Restriction map for
pFN21AE2024
(Circular) (Six-base) MAPSORT of: pFN21AE2024.seq Check: 6054 from: 1 to: 6517
pFN21AE2024 6517 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3703 3703
Size: 6517
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4327 278
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3860 1937
Size: 1923 4594
Acc65I G'GTAC_C
Cuts at: 3838 3838
Size: 6517
AclI AA'CG_TT
Cuts at: 1563 2228 4644 5017 1563
Size: 665 2416 373 3063
Fragments arranged by size:
3063 2416 665 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2278 4580 5964 6232 1632
Size: 646 2302 1384 268 1917
Fragments arranged by size:
2302 1917 1384 646 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6320
AflIII A'CryG_T
Cuts at: 1203 3008 5416 6393 1203
Size: 1805 2408 977 1327
Fragments arranged by size:
2408 1805 1327 977
AgeI A'CCGG_T
Cuts at: 5348 5348
Size: 6517
AhdI GACnn_n'nnGTC
Cuts at: 2543 5246 2543
Size: 2703 3814
AleI CACnn'nnGTG
Cuts at: 1166 3654 1166
Size: 2488 4029
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6517
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2430 2462 2430
Size: 32 6485
AlwNI CAG_nnn'CTG
Cuts at: 3588 3667 4260 5832 3588
Size: 79 593 1572 4273
Fragments arranged by size:
4273 1572 593 79
ApaLI G'TGCA_C
Cuts at: 4574 5730 4574
Size: 1156 5361
ApoI r'AATT_y
Cuts at: 1493 2495 3296 3826 4052 4204 6313 1493
Size: 1002 801 530 226 152 2109 1697
Fragments arranged by size:
2109 1697 1002 801 530 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5071 160
Size: 872 4039 1606
Fragments arranged by size:
4039 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
AvaI C'yCGr_G
Cuts at: 1870 1943 2429 3781 3842 1870
Size: 73 486 1352 61 4545
Fragments arranged by size:
4545 1352 486 73 61
BamHI G'GATC_C
Cuts at: 3180 3847 4214 3180
Size: 667 367 5483
Fragments arranged by size:
5483 667 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2270 2360 3838 5293 618
Size: 325 217 627 483 90 1478 1455 1842
Fragments arranged by size:
1842 1478 1455 627 483 325 217 90
BanII G_rGCy'C
Cuts at: 1391 2200 2592 3740 3836 1391
Size: 809 392 1148 96 4072
Fragments arranged by size:
4072 1148 809 392 96
BbeI G_GCGC'C
Cuts at: 2274 2274
Size: 6517
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2147 928
Size: 971 248 5298
Fragments arranged by size:
5298 971 248
BbvCI CC'TCA_GC
Cuts at: 2895 3351 2895
Size: 456 6061
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4708 4742 1903
Size: 34 2771 34 3678
Fragments arranged by size:
3678 2771 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4409 5619 3314
Size: 1095 1210 4212
Fragments arranged by size:
4212 1210 1095
BclI T'GATC_A
Cuts at: 1548 3028 3438 1548
Size: 1480 410 4627
Fragments arranged by size:
4627 1480 410
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5128 136
Size: 107 122 71 631 605 3456 1525
Fragments arranged by size:
3456 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3336 6512 3336
Size: 3176 3341
BlpI GC'TnA_GC
Cuts at: 3934 3934
Size: 6517
Bme1580I G_kGCm'C
Cuts at: 1790 4578 5734 1790
Size: 2788 1156 2573
Fragments arranged by size:
2788 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3011 1318
Size: 1693 4824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2623 3266 3643
Size: 416 53 83 399 24 1206 643 377
Cuts at: 3643 4248 5206 442
Size: 605 958 1753
Fragments arranged by size:
1753 1206 958 643 605 416 399 377
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6517
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2125 2268 2751 2125
Size: 143 483 5891
Fragments arranged by size:
5891 483 143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2721 5177 1239
Size: 1482 2456 2579
Fragments arranged by size:
2579 2456 1482
Bpu10I CC'TnA_GC
Cuts at: 2895 3351 2895
Size: 456 6061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2175 4642 5507 5805 2175
Size: 2467 865 298 2887
Fragments arranged by size:
2887 2467 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2530 5180 882
Size: 617 1031 2650 2219
Fragments arranged by size:
2650 2219 1031 617
BsaAI yAC'GTr
Cuts at: 493 1204 3325 4308 493
Size: 711 2121 983 2702
Fragments arranged by size:
2702 2121 983 711
BsaBI GATnn'nnATC
Cuts at: 6511 6511
Size: 6517
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2271 3198 4324 4706
Size: 53 83 186 936 738 927 1126 382
Cuts at: 4706 5341 275
Size: 635 1451
Fragments arranged by size:
1451 1126 936 927 738 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4950 5348 5622 5769 6180 4950
Size: 398 274 147 411 5287
Fragments arranged by size:
5287 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2430 2460 2872 2902 2927
Size: 30 165 30 1027 30 412 30 25
Cuts at: 2927 2957 1178
Size: 30 4738
Fragments arranged by size:
4738 1027 412 165 30 30 30 30
30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2151 2311 2314 2582 2680 3347 3368 3697
Size: 965 160 3 268 98 667 21 329
Cuts at: 3697 1186
Size: 4006
Fragments arranged by size:
4006 965 667 329 268 160 98 21
3
BseYI C'CCAG_C
Cuts at: 2350 2640 2957 3675 3731 5720 6216 2350
Size: 290 317 718 56 1989 496 2651
Fragments arranged by size:
2651 1989 718 496 317 290 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5944
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3474 3967 4728 5756 1540
Size: 275 186 1473 493 761 1028 2301
Fragments arranged by size:
2301 1473 1028 761 493 275 186
BsiHKAI G_wGCw'C
Cuts at: 2038 2200 2592 4578 4663 5734 2038
Size: 162 392 1986 85 1071 2821
Fragments arranged by size:
2821 1986 1071 392 162 85
BsmI GAATG_Cn'
Cuts at: 4028 4121 4028
Size: 93 6424
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2200 2592 3740 3836 4578 4663
Size: 399 248 162 392 1148 96 742 85
Cuts at: 4663 5734 1391
Size: 1071 2174
Fragments arranged by size:
2174 1148 1071 742 399 392 248 162
96 85
BspEI T'CCGG_A
Cuts at: 6180 6180
Size: 6517
BspHI T'CATG_A
Cuts at: 4406 6136 4406
Size: 1730 4787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 3703 3872 844
Size: 2743 116 169 3489
Fragments arranged by size:
3489 2743 169 116
BsrBI CCG'CTC
Cuts at: 1394 1823 3421 4404 1394
Size: 429 1598 983 3507
Fragments arranged by size:
3507 1598 983 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2305 2422 2922 5012 5186 65
Size: 1183 1057 117 500 2090 174 1396
Fragments arranged by size:
2090 1396 1183 1057 500 174 117
BsrFI r'CCGG_y
Cuts at: 1700 1810 5161 5348 1700
Size: 110 3351 187 2869
Fragments arranged by size:
3351 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6517
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6517
BssSI C'ACGA_G
Cuts at: 2242 4577 5589 2242
Size: 2335 1012 3170
Fragments arranged by size:
3170 2335 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6517
BstBI TT'CG_AA
Cuts at: 5321 5321
Size: 6517
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6517
BstXI CCAn_nnnn'nTGG
Cuts at: 2864 2864
Size: 6517
BstYI r'GATC_y
Cuts at: 1972 3180 3336 3847 4214 4599 4616 6068 6154
Size: 1208 156 511 367 385 17 1452 86
Cuts at: 6154 6166 6512 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1208 511 385 367 346 156
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2972 2972
Size: 6517
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5965
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3398 487
Size: 683 56 2172 3606
Fragments arranged by size:
3606 2172 683 56
BtsI GCAGTG_nn'
Cuts at: 2596 3589 4033 4827 4847 2596
Size: 993 444 794 20 4266
Fragments arranged by size:
4266 993 794 444 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6517
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6482
DraI TTT'AAA
Cuts at: 3821 3821
Size: 6517
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5524 809
Size: 507 4208 1802
Fragments arranged by size:
4208 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2610 2919 3559
Size: 54 1425 50 275 18 780 309 640
Cuts at: 3559 3964 4853 6482 8
Size: 405 889 1629 43
Fragments arranged by size:
1629 1425 889 780 640 405 309 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3964 1537
Size: 275 2152 4090
Fragments arranged by size:
4090 2152 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2317 2720 3054 4447 1353
Size: 964 403 334 1393 3423
Fragments arranged by size:
3423 1393 964 403 334
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3431 3512 5104 5478 5624 387
Size: 1064 138 1842 81 1592 374 146 1280
Fragments arranged by size:
1842 1592 1280 1064 374 146 138 81
EcoICRI GAG'CTC
Cuts at: 2198 2590 2198
Size: 392 6125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2278 2721 4580 5177 5964 6232 1239
Size: 393 646 443 1859 597 787 268 1524
Fragments arranged by size:
1859 1524 787 646 597 443 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2968 2968
Size: 6517
EcoRI G'AATT_C
Cuts at: 3296 3826 4204 3296
Size: 530 378 5609
Fragments arranged by size:
5609 530 378
EcoRV GAT'ATC
Cuts at: 2010 2010
Size: 6517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2043 2584 2043
Size: 541 5976
FspI TGC'GCA
Cuts at: 2602 5023 2602
Size: 2421 4096
HaeII r_GCGC'y
Cuts at: 1678 2274 3379 5664 1678
Size: 596 1105 2285 2531
Fragments arranged by size:
2531 2285 1105 596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2529 2561 3796 3828 5158
Size: 32 135 32 957 32 1235 32 1330
Cuts at: 5158 5190 5232 5264 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1330 1235 957 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2232 2686 3262 3774 3861 4107
Size: 988 281 294 454 576 512 87 246
Cuts at: 4107 669
Size: 3079
Fragments arranged by size:
3079 988 576 512 454 294 281 246
87
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6517
HpaI GTT'AAC
Cuts at: 2232 4107 2232
Size: 1875 4642
KasI G'GCGC_C
Cuts at: 2270 2270
Size: 6517
KpnI G_GTAC'C
Cuts at: 3842 3842
Size: 6517
MfeI C'AATT_G
Cuts at: 2487 4116 2487
Size: 1629 4888
MluI A'CGCG_T
Cuts at: 6393 6393
Size: 6517
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2189 5631 5815 1305
Size: 884 3442 184 2007
Fragments arranged by size:
3442 2007 884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2612 2921 10
Size: 54 1425 343 780 309 3606
Fragments arranged by size:
3606 1425 780 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3654 4475 4834 4993 518
Size: 648 2488 821 359 159 2042
Fragments arranged by size:
2488 2042 821 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2344 2653 2827 3726 3933 4610 5758 6003
Size: 738 309 174 899 207 677 1148 245
Cuts at: 6003 1606
Size: 2120
Fragments arranged by size:
2120 1148 899 738 677 309 245 207
174
NarI GG'CG_CC
Cuts at: 2271 2271
Size: 6517
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5965
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6517
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6517
NotI GC'GGCC_GC
Cuts at: 3964 3964
Size: 6517
NspI r_CATG'y
Cuts at: 1132 1229 3875 5420 1132
Size: 97 2646 1545 2229
Fragments arranged by size:
2646 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6517
PciI A'CATG_T
Cuts at: 5416 5416
Size: 6517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2288 2357 1108
Size: 1180 69 5268
Fragments arranged by size:
5268 1180 69
PmeI GTTT'AAAC
Cuts at: 3821 3821
Size: 6517
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2430 2462 4586 4618 6099 6131 2430
Size: 32 2124 32 1481 32 2816
Fragments arranged by size:
2816 2124 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2968 2968
Size: 6517
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6517
PsiI TTA'TAA
Cuts at: 4087 4087
Size: 6517
PstI C_TGCA'G
Cuts at: 830 2406 2583 3399 3584 3672 3700 3869 830
Size: 1576 177 816 185 88 28 169 3478
Fragments arranged by size:
3478 1576 816 185 177 169 88 28
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6517
PvuII CAG'CTG
Cuts at: 2344 2653 2344
Size: 309 6208
SacI G_AGCT'C
Cuts at: 2200 2592 2200
Size: 392 6125
SalI G'TCGA_C
Cuts at: 1936 3859 1936
Size: 1923 4594
SbfI CC_TGCA'GG
Cuts at: 3672 3869 3672
Size: 197 6320
ScaI AGT'ACT
Cuts at: 4765 6477 4765
Size: 1712 4805
SfcI C'TryA_G
Cuts at: 826 1045 2256 2402 2579 2935 3395 3580 3668
Size: 219 1211 146 177 356 460 185 88
Cuts at: 3668 3696 3865 5000 5681 5872 826
Size: 28 169 1135 681 191 1471
Fragments arranged by size:
1471 1211 1135 681 460 356 219 191
185 177 169 146 88 28
SfoI GGC'GCC
Cuts at: 2272 2272
Size: 6517
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6517
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6517
SmaI CCC'GGG
Cuts at: 1872 3783 3844 1872
Size: 1911 61 4545
Fragments arranged by size:
4545 1911 61
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2190 4621 5522 5784 820
Size: 197 926 247 2431 901 262 1553
Fragments arranged by size:
2431 1553 926 901 262 247 197
SnaBI TAC'GTA
Cuts at: 493 4308 493
Size: 3815 2702
SpeI A'CTAG_T
Cuts at: 152 2467 152
Size: 2315 4202
SphI G_CATG'C
Cuts at: 1132 3875 1132
Size: 2743 3774
SspI AAT'ATT
Cuts at: 5 52 2084 4441 5
Size: 47 2032 2357 2081
Fragments arranged by size:
2357 2081 2032 47
StuI AGG'CCT
Cuts at: 1517 3310 1517
Size: 1793 4724
StyI C'CwwG_G
Cuts at: 513 1065 3956 513
Size: 552 2891 3074
Fragments arranged by size:
3074 2891 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2299 4544 4561 4714 4899 5344 325
Size: 1255 719 2245 17 153 185 445 1498
Fragments arranged by size:
2245 1498 1255 719 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4763 6475 96
Size: 274 80 33 51 1443 2786 1712 138
Fragments arranged by size:
2786 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6477 6509 6477
Size: 32 6485
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6517
XbaI T'CTAG_A
Cuts at: 3853 3853
Size: 6517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3336 3653 3336
Size: 317 6200
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6517
XmaI C'CCGG_G
Cuts at: 1870 3781 3842 1870
Size: 1911 61 4545
Fragments arranged by size:
4545 1911 61
XmnI GAAnn'nnTTC
Cuts at: 2209 2387 2445 2496 3300 3790 4646 2209
Size: 178 58 51 804 490 856 4080
Fragments arranged by size:
4080 856 804 490 178 58 51
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4325 276
Size: 53 83 186 936 2791 2468
Fragments arranged by size:
2791 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SapI SexAI SfiI SrfI SwaI