Restriction map for
pFN21AE2022
(Circular) (Six-base) MAPSORT of: pFN21AE2022.seq Check: 3275 from: 1 to: 6502
pFN21AE2022 6502 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2223 2223
Size: 6502
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2383 3845 1937
Size: 446 1462 4594
Fragments arranged by size:
4594 1462 446
Acc65I G'GTAC_C
Cuts at: 2256 3823 2256
Size: 1567 4935
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4565 5949 6217 1632
Size: 2933 1384 268 1917
Fragments arranged by size:
2933 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 5401 6378 1203
Size: 4198 977 1327
Fragments arranged by size:
4198 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AlwNI CAG_nnn'CTG
Cuts at: 2912 3156 4245 5817 2912
Size: 244 1089 1572 3597
Fragments arranged by size:
3597 1572 1089 244
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 2126 3811 4037 4189 6298 1493
Size: 633 1685 226 152 2109 1697
Fragments arranged by size:
2109 1697 1685 633 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 3827 1870
Size: 73 1884 4545
Fragments arranged by size:
4545 1884 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2314 2347 2314
Size: 33 6469
BamHI G'GATC_C
Cuts at: 2767 3832 4199 2767
Size: 1065 367 5070
Fragments arranged by size:
5070 1065 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2256 3101 3823 5278 618
Size: 325 217 627 469 845 722 1455 1842
Fragments arranged by size:
1842 1455 845 722 627 469 325 217
BanII G_rGCy'C
Cuts at: 1391 2405 3260 3452 3785 3821 1391
Size: 1014 855 192 333 36 4072
Fragments arranged by size:
4072 1014 855 333 192 36
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3405 928
Size: 971 1506 4025
Fragments arranged by size:
4025 1506 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2602 2636 4693 4727 1903
Size: 34 50 34 581 34 2057 34 3678
Fragments arranged by size:
3678 2057 581 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2677 3250 4394 5604 2677
Size: 573 1144 1210 3575
Fragments arranged by size:
3575 1210 1144 573
BclI T'GATC_A
Cuts at: 1548 3514 1548
Size: 1966 4536
BfrBI ATG'CAT
Cuts at: 2462 2462
Size: 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3128 5113 136
Size: 107 122 71 631 605 1456 1985 1525
Fragments arranged by size:
1985 1525 1456 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 3919 3919
Size: 6502
Bme1580I G_kGCm'C
Cuts at: 1790 2657 3142 4563 5719 1790
Size: 867 485 1421 1156 2573
Fragments arranged by size:
2573 1421 1156 867 485
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2526 3040 3465
Size: 416 53 83 399 24 1109 514 425
Cuts at: 3465 3783 4233 5191 442
Size: 318 450 958 1753
Fragments arranged by size:
1753 1109 958 514 450 425 416 399
318 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3098
Size: 6502
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3363 5162 1239
Size: 2124 1799 2579
Fragments arranged by size:
2579 2124 1799
Bpu10I CC'TnA_GC
Cuts at: 2208 3308 2208
Size: 1100 5402
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3466 4627 5492 5790 3466
Size: 1161 865 298 4178
Fragments arranged by size:
4178 1161 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5165 882
Size: 617 3666 2219
Fragments arranged by size:
3666 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3708 4293 493
Size: 711 2504 585 2702
Fragments arranged by size:
2702 2504 711 585
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4309 4691 5326 275
Size: 53 83 186 936 2776 382 635 1451
Fragments arranged by size:
2776 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3180 4935 5333 5607 5754 6165 3180
Size: 1755 398 274 147 411 3517
Fragments arranged by size:
3517 1755 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6277
Fragments arranged by size:
6277 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2585 3087 3134 3399 3793 1186
Size: 1399 502 47 265 394 3895
Fragments arranged by size:
3895 1399 502 394 265 47
BseYI C'CCAG_C
Cuts at: 3786 5705 6201 3786
Size: 1919 496 4087
Fragments arranged by size:
4087 1919 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5929
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3952 4713 5741 1540
Size: 275 186 1951 761 1028 2301
Fragments arranged by size:
2301 1951 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 2513 3084 3260 3785 4563 4648 5719 2214
Size: 299 571 176 525 778 85 1071 2997
Fragments arranged by size:
2997 1071 778 571 525 299 176 85
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2405 2513 2657 3084 3142 3260
Size: 399 424 191 108 144 427 58 118
Cuts at: 3260 3452 3785 3821 4563 4648 5719 1391
Size: 192 333 36 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 424 399 333 192
191 144 118 108 85 58 36
BspEI T'CCGG_A
Cuts at: 6165 6165
Size: 6502
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2223 3857 844
Size: 1379 1634 3489
Fragments arranged by size:
3489 1634 1379
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5146 5333 1700
Size: 110 3336 187 2869
Fragments arranged by size:
3336 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3087 96
Size: 2991 3511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6502
BssSI C'ACGA_G
Cuts at: 3581 4562 5574 3581
Size: 981 1012 4509
Fragments arranged by size:
4509 1012 981
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6502
BstBI TT'CG_AA
Cuts at: 2270 5306 2270
Size: 3036 3466
BstEII G'GTnAC_C
Cuts at: 1184 2569 1184
Size: 1385 5117
BstYI r'GATC_y
Cuts at: 1972 2278 2767 3832 4199 4584 4601 6053 6139
Size: 306 489 1065 367 385 17 1452 86
Cuts at: 6139 6151 6497 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1065 489 385 367 346 306
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2913 3792 2913
Size: 879 5623
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5763
Fragments arranged by size:
5763 683 56
BtsI GCAGTG_nn'
Cuts at: 3501 4018 4812 4832 3501
Size: 517 794 20 5171
Fragments arranged by size:
5171 794 517 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 2940 3806 2940
Size: 866 5636
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5509 809
Size: 507 4193 1802
Fragments arranged by size:
4193 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3120 3949 4838
Size: 54 1425 50 275 18 1290 829 889
Cuts at: 4838 6467 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1290 889 829 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3949 1537
Size: 275 2137 4090
Fragments arranged by size:
4090 2137 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 3424 4432 1353
Size: 762 1309 1008 3423
Fragments arranged by size:
3423 1309 1008 762
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5089 5463 5609 387
Size: 1064 138 3500 374 146 1280
Fragments arranged by size:
3500 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3258 3783 3258
Size: 525 5977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3363 4565 5162 5949 6217 1239
Size: 393 1731 1202 597 787 268 1524
Fragments arranged by size:
1731 1524 1202 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3111 3111
Size: 6502
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2516 2813 2516
Size: 297 6205
FspI TGC'GCA
Cuts at: 5008 5008
Size: 6502
HaeII r_GCGC'y
Cuts at: 1678 5649 1678
Size: 3971 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3184 3216 3459 3491 3551
Size: 32 135 32 1612 32 243 32 60
Cuts at: 3551 3583 5143 5175 5217 5249 1373
Size: 32 1560 32 42 32 2626
Fragments arranged by size:
2626 1612 1560 243 135 60 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KpnI G_GTAC'C
Cuts at: 2260 3827 2260
Size: 1567 4935
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2661 3079 5616 5800 1305
Size: 1356 418 2537 184 2007
Fragments arranged by size:
2537 2007 1356 418 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4680
Fragments arranged by size:
4680 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2662 3683 4460 4819 4978 518
Size: 648 1496 1021 777 359 159 2042
Fragments arranged by size:
2042 1496 1021 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2541 2567 3918 4595 5743 5988 1606
Size: 935 26 1351 677 1148 245 2120
Fragments arranged by size:
2120 1351 1148 935 677 245 26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5950
NdeI CA'TA_TG
Cuts at: 387 2659 387
Size: 2272 4230
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NsiI A_TGCA'T
Cuts at: 2464 2464
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 3682 3860 5405 1132
Size: 97 2453 178 1545 2229
Fragments arranged by size:
2453 2229 1545 178 97
PacI TTA_AT'TAA
Cuts at: 3540 3540
Size: 6502
PasI CC'CwG_GG
Cuts at: 1348 2351 1348
Size: 1003 5499
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6502
PfoI T'CCnGG_A
Cuts at: 3379 3379
Size: 6502
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PmlI CAC'GTG
Cuts at: 3708 3708
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2700 2732 4571 4603 6084 6116 2700
Size: 32 1839 32 1481 32 3086
Fragments arranged by size:
3086 1839 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3111 3111
Size: 6502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PstI C_TGCA'G
Cuts at: 830 3854 830
Size: 3024 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2541 2541
Size: 6502
SacI G_AGCT'C
Cuts at: 3260 3785 3260
Size: 525 5977
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SanDI GG'GwC_CC
Cuts at: 3111 3111
Size: 6502
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 4750 6462 4750
Size: 1712 4790
SexAI A'CCwGG_T
Cuts at: 2573 2573
Size: 6502
SfcI C'TryA_G
Cuts at: 826 1045 3148 3850 4985 5666 5857 826
Size: 219 2103 702 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 702 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6502
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3445 4606 5507 5769 820
Size: 197 926 1502 1161 901 262 1553
Fragments arranged by size:
1553 1502 1161 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 2068 3438 4426 5
Size: 47 2016 1370 988 2081
Fragments arranged by size:
2081 2016 1370 988 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6502
StyI C'CwwG_G
Cuts at: 513 1065 3507 3941 513
Size: 552 2442 434 3074
Fragments arranged by size:
3074 2442 552 434
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4529 4546 4699 4884 5329 325
Size: 1255 2949 17 153 185 445 1498
Fragments arranged by size:
2949 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3087 4748 6460
Size: 274 80 33 51 1443 1110 1661 1712
Cuts at: 6460 96
Size: 138
Fragments arranged by size:
1712 1661 1443 1110 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6462 6494 6462
Size: 32 6470
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2571 3029 3112 2011
Size: 560 458 83 5401
Fragments arranged by size:
5401 560 458 83
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6502
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PspOMI PspXI PsrI
RsrII SacII SapI SfiI SfoI SrfI SwaI