Restriction map for
pFN21AE2019
(Circular) (Six-base) MAPSORT of: pFN21AE2019.seq Check: 9364 from: 1 to: 6493
pFN21AE2019 6493 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4303 278
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2308 2861 3836 1937
Size: 371 553 975 4594
Fragments arranged by size:
4594 975 553 371
Acc65I G'GTAC_C
Cuts at: 3814 3814
Size: 6493
AclI AA'CG_TT
Cuts at: 1563 4620 4993 1563
Size: 3057 373 3063
Fragments arranged by size:
3063 3057 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3723 4556 5940 6208 1632
Size: 2091 833 1384 268 1917
Fragments arranged by size:
2091 1917 1384 833 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6296
AflIII A'CryG_T
Cuts at: 1203 5392 6369 1203
Size: 4189 977 1327
Fragments arranged by size:
4189 1327 977
AgeI A'CCGG_T
Cuts at: 5324 5324
Size: 6493
AhdI GACnn_n'nnGTC
Cuts at: 5222 5222
Size: 6493
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6493
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2275 2546 2590 3080 1184
Size: 1091 271 44 490 4597
Fragments arranged by size:
4597 1091 490 271 44
AlwNI CAG_nnn'CTG
Cuts at: 4236 5808 4236
Size: 1572 4921
ApaI G_GGCC'C
Cuts at: 2127 2127
Size: 6493
ApaLI G'TGCA_C
Cuts at: 4550 5706 4550
Size: 1156 5337
ApoI r'AATT_y
Cuts at: 1493 2411 2480 3802 4028 4180 6289 1493
Size: 918 69 1322 226 152 2109 1697
Fragments arranged by size:
2109 1697 1322 918 226 152 69
AseI AT'TA_AT
Cuts at: 160 1032 2892 5047 160
Size: 872 1860 2155 1606
Fragments arranged by size:
2155 1860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 3818 1870
Size: 73 177 1698 4545
Fragments arranged by size:
4545 1698 177 73
BamHI G'GATC_C
Cuts at: 3823 4190 3823
Size: 367 6126
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3814 5269 618
Size: 325 217 627 2027 1455 1842
Fragments arranged by size:
2027 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2127 3812 1391
Size: 736 1685 4072
Fragments arranged by size:
4072 1685 736
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3689 928
Size: 971 1790 3732
Fragments arranged by size:
3732 1790 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4684 4718 1903
Size: 34 2747 34 3678
Fragments arranged by size:
3678 2747 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4385 5595 4385
Size: 1210 5283
BclI T'GATC_A
Cuts at: 1548 3667 1548
Size: 2119 4374
BfrBI ATG'CAT
Cuts at: 3454 3502 3556 3454
Size: 48 54 6391
Fragments arranged by size:
6391 54 48
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5104 136
Size: 107 122 71 631 605 3432 1525
Fragments arranged by size:
3432 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6488 6488
Size: 6493
BlpI GC'TnA_GC
Cuts at: 3372 3910 3372
Size: 538 5955
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2737 4554 5710 1790
Size: 337 610 1817 1156 2573
Fragments arranged by size:
2573 1817 1156 610 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6493
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2138 4224 5182
Size: 416 53 83 399 24 721 2086 958
Cuts at: 5182 442
Size: 1753
Fragments arranged by size:
2086 1753 958 721 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6493
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3697 3106
Size: 591 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 3012 5153 1239
Size: 1496 277 2141 2579
Fragments arranged by size:
2579 2141 1496 277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2836 4618 5483 5781 2836
Size: 1782 865 298 3548
Fragments arranged by size:
3548 1782 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5156 882
Size: 617 3657 2219
Fragments arranged by size:
3657 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4284 493
Size: 711 3080 2702
Fragments arranged by size:
3080 2702 711
BsaBI GATnn'nnATC
Cuts at: 6487 6487
Size: 6493
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4300 4682 5317 275
Size: 53 83 186 936 2767 382 635 1451
Fragments arranged by size:
2767 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2556 4926 5324 5598 5745 6156 2556
Size: 2370 398 274 147 411 2893
Fragments arranged by size:
2893 2370 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2095 2125 2351 2381 2733
Size: 30 165 30 692 30 226 30 352
Cuts at: 2733 2763 1178
Size: 30 4908
Fragments arranged by size:
4908 692 352 226 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6493
BseYI C'CCAG_C
Cuts at: 3067 5696 6192 3067
Size: 2629 496 3368
Fragments arranged by size:
3368 2629 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2586 3095 1163
Size: 573 850 509 4561
Fragments arranged by size:
4561 850 573 509
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 3943 4704 5732 1540
Size: 275 186 730 1212 761 1028 2301
Fragments arranged by size:
2301 1212 1028 761 730 275 186
BsiHKAI G_wGCw'C
Cuts at: 4554 4639 5710 4554
Size: 85 1071 5337
Fragments arranged by size:
5337 1071 85
BsmI GAATG_Cn'
Cuts at: 4004 4097 4004
Size: 93 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2737 3812 4554 4639 5710 1391
Size: 399 337 610 1075 742 85 1071 2174
Fragments arranged by size:
2174 1075 1071 742 610 399 337 85
BspEI T'CCGG_A
Cuts at: 6156 6156
Size: 6493
BspHI T'CATG_A
Cuts at: 3670 4382 6112 3670
Size: 712 1730 4051
Fragments arranged by size:
4051 1730 712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2582 2612 3848 844
Size: 1738 30 1236 3489
Fragments arranged by size:
3489 1738 1236 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2727 4380 1394
Size: 429 904 1653 3507
Fragments arranged by size:
3507 1653 904 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4988 5162 65
Size: 1183 3740 174 1396
Fragments arranged by size:
3740 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 5137 5324 1700
Size: 110 224 3103 187 2869
Fragments arranged by size:
3103 2869 224 187 110
BsrGI T'GTAC_A
Cuts at: 96 3720 96
Size: 3624 2869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6493
BssSI C'ACGA_G
Cuts at: 4553 5565 4553
Size: 1012 5481
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2627 794
Size: 1833 4660
BstBI TT'CG_AA
Cuts at: 5297 5297
Size: 6493
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6493
BstXI CCAn_nnnn'nTGG
Cuts at: 3020 3020
Size: 6493
BstYI r'GATC_y
Cuts at: 1972 2238 3823 4190 4575 4592 6044 6130 6142
Size: 266 1585 367 385 17 1452 86 12
Cuts at: 6142 6488 1972
Size: 346 1977
Fragments arranged by size:
1977 1585 1452 385 367 346 266 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5941
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5754
Fragments arranged by size:
5754 683 56
BtsI GCAGTG_nn'
Cuts at: 2247 2724 2735 2997 3366 4009 4803 4823 2247
Size: 477 11 262 369 643 794 20 3917
Fragments arranged by size:
3917 794 643 477 369 262 20 11
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6493
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6458
DraI TTT'AAA
Cuts at: 3797 3797
Size: 6493
DraIII CAC_nnn'GTG
Cuts at: 3640 3640
Size: 6493
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5500 809
Size: 507 4184 1802
Fragments arranged by size:
4184 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2614 2728 3039
Size: 54 1425 50 275 18 784 114 311
Cuts at: 3039 3940 4829 6458 8
Size: 901 889 1629 43
Fragments arranged by size:
1629 1425 901 889 784 311 275 114
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2728 3940 1537
Size: 275 916 1212 4090
Fragments arranged by size:
4090 1212 916 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3075 3699 4423 1353
Size: 1722 624 724 3423
Fragments arranged by size:
3423 1722 724 624
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5080 5454 5600 387
Size: 1064 138 3491 374 146 1280
Fragments arranged by size:
3491 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2735 3012 3723 4556 5153 5940 6208
Size: 393 1103 277 711 833 597 787 268
Cuts at: 6208 1239
Size: 1524
Fragments arranged by size:
1524 1103 833 787 711 597 393 277
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2347 2902 2347
Size: 555 5938
EcoRI G'AATT_C
Cuts at: 3802 4180 3802
Size: 378 6115
FspI TGC'GCA
Cuts at: 4999 4999
Size: 6493
HaeII r_GCGC'y
Cuts at: 1678 3679 5640 1678
Size: 2001 1961 2531
Fragments arranged by size:
2531 2001 1961
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3652 3684 5134 5166 5208
Size: 32 135 32 2080 32 1450 32 42
Cuts at: 5208 5240 1373
Size: 32 2626
Fragments arranged by size:
2626 2080 1450 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2309 2862 3028 3837 4083 669
Size: 988 281 371 553 166 809 246 3079
Fragments arranged by size:
3079 988 809 553 371 281 246 166
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6493
HpaI GTT'AAC
Cuts at: 4083 4083
Size: 6493
KpnI G_GTAC'C
Cuts at: 3818 3818
Size: 6493
MfeI C'AATT_G
Cuts at: 4092 4092
Size: 6493
MluI A'CGCG_T
Cuts at: 6369 6369
Size: 6493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5607 5791 1305
Size: 4302 184 2007
Fragments arranged by size:
4302 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2616 3041 10
Size: 54 1425 343 784 425 3462
Fragments arranged by size:
3462 1425 784 425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2769 3018 4451 4810 4969 518
Size: 648 1603 249 1433 359 159 2042
Fragments arranged by size:
2042 1603 1433 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2530 3909 4586 5734 5979 1606
Size: 924 1379 677 1148 245 2120
Fragments arranged by size:
2120 1379 1148 924 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5941
NdeI CA'TA_TG
Cuts at: 387 2335 387
Size: 1948 4545
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6493
NotI GC'GGCC_GC
Cuts at: 3940 3940
Size: 6493
NsiI A_TGCA'T
Cuts at: 3456 3504 3558 3456
Size: 48 54 6391
Fragments arranged by size:
6391 54 48
NspI r_CATG'y
Cuts at: 1132 1229 2256 3502 3851 5396 1132
Size: 97 1027 1246 349 1545 2229
Fragments arranged by size:
2229 1545 1246 1027 349 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6493
PciI A'CATG_T
Cuts at: 5392 5392
Size: 6493
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3640 1108
Size: 2532 3961
PmeI GTTT'AAAC
Cuts at: 3797 3797
Size: 6493
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 6075 6107 4562
Size: 32 1481 32 4948
Fragments arranged by size:
4948 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6493
PsiI TTA'TAA
Cuts at: 4063 4063
Size: 6493
PspOMI G'GGCC_C
Cuts at: 2123 2123
Size: 6493
PstI C_TGCA'G
Cuts at: 830 3845 830
Size: 3015 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6493
PvuII CAG'CTG
Cuts at: 2530 2530
Size: 6493
SalI G'TCGA_C
Cuts at: 1936 2307 2860 3835 1936
Size: 371 553 975 4594
Fragments arranged by size:
4594 975 553 371
SapI GCTCTTCn'nnn_
Cuts at: 3699 3699
Size: 6493
SbfI CC_TGCA'GG
Cuts at: 3845 3845
Size: 6493
ScaI AGT'ACT
Cuts at: 4741 6453 4741
Size: 1712 4781
SexAI A'CCwGG_T
Cuts at: 2967 2967
Size: 6493
SfcI C'TryA_G
Cuts at: 826 1045 3841 4976 5657 5848 826
Size: 219 2796 1135 681 191 1471
Fragments arranged by size:
2796 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6493
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6493
SmaI CCC'GGG
Cuts at: 1872 2122 3820 1872
Size: 250 1698 4545
Fragments arranged by size:
4545 1698 250
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2815 4597 5498 5760 820
Size: 197 926 872 1782 901 262 1553
Fragments arranged by size:
1782 1553 926 901 872 262 197
SnaBI TAC'GTA
Cuts at: 493 4284 493
Size: 3791 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6493
SphI G_CATG'C
Cuts at: 1132 3502 3851 1132
Size: 2370 349 3774
Fragments arranged by size:
3774 2370 349
SspI AAT'ATT
Cuts at: 5 52 4417 5
Size: 47 4365 2081
Fragments arranged by size:
4365 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6493
StyI C'CwwG_G
Cuts at: 513 1065 2380 2509 2925 3932 513
Size: 552 1315 129 416 1007 3074
Fragments arranged by size:
3074 1315 1007 552 416 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2393 2560 3059 3470 4520 4537 4690
Size: 1255 813 167 499 411 1050 17 153
Cuts at: 4690 4875 5320 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1050 813 499 445 411 185
167 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 2802 3720
Size: 274 80 33 51 1443 396 429 918
Cuts at: 3720 4739 6451 96
Size: 1019 1712 138
Fragments arranged by size:
1712 1443 1019 918 429 396 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2095 2127 2311 2343 2351 2383 3466 3498 6453
Size: 32 184 32 8 32 1083 32 2955
Cuts at: 6453 6485 2095
Size: 32 2103
Fragments arranged by size:
2955 2103 1083 184 32 32 32 32
32 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6493
XbaI T'CTAG_A
Cuts at: 3829 3829
Size: 6493
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2361 3004 2361
Size: 643 5850
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6493
XmaI C'CCGG_G
Cuts at: 1870 2120 3818 1870
Size: 250 1698 4545
Fragments arranged by size:
4545 1698 250
XmnI GAAnn'nnTTC
Cuts at: 4622 4622
Size: 6493
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4301 276
Size: 53 83 186 936 2767 2468
Fragments arranged by size:
2767 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI