Restriction map for
pFN21AE2018
(Circular) (Six-base) MAPSORT of: pFN21AE2018.seq Check: 711 from: 1 to: 6487
pFN21AE2018 6487 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4297 278
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2447 3055 3758 3830 1937
Size: 510 608 703 72 4594
Fragments arranged by size:
4594 703 608 510 72
Acc65I G'GTAC_C
Cuts at: 3808 3808
Size: 6487
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2006 2366 2712 3152 3786 4550 5934 6202
Size: 374 360 346 440 634 764 1384 268
Cuts at: 6202 1632
Size: 1917
Fragments arranged by size:
1917 1384 764 634 440 374 360 346
268
AflII C'TTAA_G
Cuts at: 820 1017 2923 3668 820
Size: 197 1906 745 3639
Fragments arranged by size:
3639 1906 745 197
AflIII A'CryG_T
Cuts at: 1203 3291 3308 5386 6363 1203
Size: 2088 17 2078 977 1327
Fragments arranged by size:
2088 2078 1327 977 17
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 5216 5216
Size: 6487
AleI CACnn'nnGTG
Cuts at: 1166 3246 1166
Size: 2080 4407
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6487
AlwNI CAG_nnn'CTG
Cuts at: 4230 5802 4230
Size: 1572 4915
ApaLI G'TGCA_C
Cuts at: 4544 5700 4544
Size: 1156 5331
ApoI r'AATT_y
Cuts at: 1493 2633 2822 3430 3796 4022 4174 6283 1493
Size: 1140 189 608 366 226 152 2109 1697
Fragments arranged by size:
2109 1697 1140 608 366 226 189 152
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 3723 3812 1870
Size: 73 1780 89 4545
Fragments arranged by size:
4545 1780 89 73
BamHI G'GATC_C
Cuts at: 3817 4184 3817
Size: 367 6120
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3808 5263 618
Size: 325 217 627 2021 1455 1842
Fragments arranged by size:
2021 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2343 3806 1391
Size: 952 1463 4072
Fragments arranged by size:
4072 1463 952
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3051 928
Size: 971 1152 4364
Fragments arranged by size:
4364 1152 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4678 4712 1903
Size: 34 2741 34 3678
Fragments arranged by size:
3678 2741 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4379 5589 4379
Size: 1210 5277
BclI T'GATC_A
Cuts at: 1548 2905 1548
Size: 1357 5130
BfrBI ATG'CAT
Cuts at: 2112 2112
Size: 6487
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5098 136
Size: 107 122 71 631 605 3426 1525
Fragments arranged by size:
3426 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3607 6482 3607
Size: 2875 3612
BlpI GC'TnA_GC
Cuts at: 3904 3904
Size: 6487
Bme1580I G_kGCm'C
Cuts at: 1790 2127 2961 4548 5704 1790
Size: 337 834 1587 1156 2573
Fragments arranged by size:
2573 1587 1156 834 337
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6487
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4218 5176 442
Size: 416 53 83 399 24 2801 958 1753
Fragments arranged by size:
2801 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3041 5147 1239
Size: 1802 2106 2579
Fragments arranged by size:
2579 2106 1802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4612 5477 5775 4612
Size: 865 298 5324
Fragments arranged by size:
5324 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5150 882
Size: 617 3651 2219
Fragments arranged by size:
3651 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3311 4278 493
Size: 711 2107 967 2702
Fragments arranged by size:
2702 2107 967 711
BsaBI GATnn'nnATC
Cuts at: 6481 6481
Size: 6487
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4294 4676 5311 275
Size: 53 83 186 936 2761 382 635 1451
Fragments arranged by size:
2761 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4920 5318 5592 5739 6150 4920
Size: 398 274 147 411 5257
Fragments arranged by size:
5257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3354 3384 1178
Size: 30 165 30 1951 30 4281
Fragments arranged by size:
4281 1951 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6487
BseYI C'CCAG_C
Cuts at: 2099 5690 6186 2099
Size: 3591 496 2400
Fragments arranged by size:
3591 2400 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3402 1163
Size: 573 1666 4248
Fragments arranged by size:
4248 1666 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3937 4698 5726 1540
Size: 275 186 1936 761 1028 2301
Fragments arranged by size:
2301 1936 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4548 4633 5704 4548
Size: 85 1071 5331
Fragments arranged by size:
5331 1071 85
BsmI GAATG_Cn'
Cuts at: 3679 3998 4091 3679
Size: 319 93 6075
Fragments arranged by size:
6075 319 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2343 2961 3806 4548 4633 5704
Size: 399 337 216 618 845 742 85 1071
Cuts at: 5704 1391
Size: 2174
Fragments arranged by size:
2174 1071 845 742 618 399 337 216
85
BspEI T'CCGG_A
Cuts at: 6150 6150
Size: 6487
BspHI T'CATG_A
Cuts at: 4376 6106 4376
Size: 1730 4757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3842 844
Size: 2998 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4374 1394
Size: 429 2551 3507
Fragments arranged by size:
3507 2551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4982 5156 65
Size: 1183 3734 174 1396
Fragments arranged by size:
3734 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5131 5318 1700
Size: 110 3321 187 2869
Fragments arranged by size:
3321 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2887 96
Size: 2791 3696
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6487
BssSI C'ACGA_G
Cuts at: 4547 5559 4547
Size: 1012 5475
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6487
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6487
BstXI CCAn_nnnn'nTGG
Cuts at: 3010 3010
Size: 6487
BstYI r'GATC_y
Cuts at: 1972 3607 3639 3817 4184 4569 4586 6038 6124
Size: 1635 32 178 367 385 17 1452 86
Cuts at: 6124 6136 6482 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1635 1452 385 367 346 178 86
32 17 12
Bsu36I CC'TnA_GG
Cuts at: 3099 3099
Size: 6487
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4996
Fragments arranged by size:
4996 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5748
Fragments arranged by size:
5748 683 56
BtsI GCAGTG_nn'
Cuts at: 4003 4797 4817 4003
Size: 794 20 5673
Fragments arranged by size:
5673 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6452
DraI TTT'AAA
Cuts at: 2068 3791 2068
Size: 1723 4764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5494 809
Size: 507 4178 1802
Fragments arranged by size:
4178 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3934 4823 6452
Size: 54 1425 50 275 18 2104 889 1629
Cuts at: 6452 8
Size: 43
Fragments arranged by size:
2104 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3934 1537
Size: 275 2122 4090
Fragments arranged by size:
4090 2122 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2049 2067 2331 2475 2982 3630 4417 1353
Size: 696 18 264 144 507 648 787 3423
Fragments arranged by size:
3423 787 696 648 507 264 144 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5074 5448 5594 387
Size: 1064 138 3485 374 146 1280
Fragments arranged by size:
3485 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2006 2366 2712 3041 3152 3786 4550
Size: 393 374 360 346 329 111 634 764
Cuts at: 4550 5147 5934 6202 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 764 634 597 393 374 360
346 329 268 111
EcoO109I rG'GnC_Cy
Cuts at: 3673 3673
Size: 6487
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
EcoRV GAT'ATC
Cuts at: 2358 2358
Size: 6487
FspI TGC'GCA
Cuts at: 4993 4993
Size: 6487
HaeII r_GCGC'y
Cuts at: 1678 5634 1678
Size: 3956 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2031 2063 2770 2802 3624
Size: 32 135 32 459 32 707 32 822
Cuts at: 3624 3656 5128 5160 5202 5234 1373
Size: 32 1472 32 42 32 2626
Fragments arranged by size:
2626 1472 822 707 459 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3831 4077 669
Size: 988 281 1893 246 3079
Fragments arranged by size:
3079 1893 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2647 748
Size: 1899 4588
HpaI GTT'AAC
Cuts at: 4077 4077
Size: 6487
KpnI G_GTAC'C
Cuts at: 3812 3812
Size: 6487
MfeI C'AATT_G
Cuts at: 2430 2754 4086 2430
Size: 324 1332 4831
Fragments arranged by size:
4831 1332 324
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3168 5601 5785 1305
Size: 1863 2433 184 2007
Fragments arranged by size:
2433 2007 1863 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4665
Fragments arranged by size:
4665 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3246 4445 4804 4963 518
Size: 648 2080 1199 359 159 2042
Fragments arranged by size:
2080 2042 1199 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3172 3903 4580 5728 5973 1606
Size: 1566 731 677 1148 245 2120
Fragments arranged by size:
2120 1566 1148 731 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4996
Fragments arranged by size:
4996 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6487
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NsiI A_TGCA'T
Cuts at: 2114 2114
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 3295 3845 5390 1132
Size: 97 2066 550 1545 2229
Fragments arranged by size:
2229 2066 1545 550 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 3291 5386 3291
Size: 2095 4392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6487
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 6069 6101 4556
Size: 32 1481 32 4942
Fragments arranged by size:
4942 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3673 3673
Size: 6487
PshAI GACnn'nnGTC
Cuts at: 1613 3051 1613
Size: 1438 5049
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PspXI vC'TCGA_Gb
Cuts at: 3723 3723
Size: 6487
PstI C_TGCA'G
Cuts at: 830 3839 830
Size: 3009 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
PvuII CAG'CTG
Cuts at: 3172 3172
Size: 6487
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SapI GCTCTTCn'nnn_
Cuts at: 2049 2331 2049
Size: 282 6205
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 3068 4735 6447 3068
Size: 1667 1712 3108
Fragments arranged by size:
3108 1712 1667
SfcI C'TryA_G
Cuts at: 826 1045 2010 3835 4970 5651 5842 826
Size: 219 965 1825 1135 681 191 1471
Fragments arranged by size:
1825 1471 1135 965 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 3814 1872
Size: 1942 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2923 3668 3723 4591 5492 5754
Size: 197 926 980 745 55 868 901 262
Cuts at: 5754 820
Size: 1553
Fragments arranged by size:
1553 980 926 901 868 745 262 197
55
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3845 1132
Size: 2713 3774
SspI AAT'ATT
Cuts at: 5 52 3505 3775 4411 5
Size: 47 3453 270 636 2081
Fragments arranged by size:
3453 2081 636 270 47
StuI AGG'CCT
Cuts at: 1517 2540 1517
Size: 1023 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 3926 513
Size: 552 939 1922 3074
Fragments arranged by size:
3074 1922 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4514 4531 4684 4869 5314 325
Size: 1255 2934 17 153 185 445 1498
Fragments arranged by size:
2934 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2887 3066 4733
Size: 274 80 33 51 1443 910 179 1667
Cuts at: 4733 6445 96
Size: 1712 138
Fragments arranged by size:
1712 1667 1443 910 274 179 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6447 6479 6447
Size: 32 6455
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6487
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XhoI C'TCGA_G
Cuts at: 1943 3723 1943
Size: 1780 4707
XmaI C'CCGG_G
Cuts at: 1870 3812 1870
Size: 1942 4545
XmnI GAAnn'nnTTC
Cuts at: 2148 3515 3714 4616 2148
Size: 1367 199 902 4019
Fragments arranged by size:
4019 1367 902 199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4295 276
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PsrI RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI