Restriction map for
pFN21AE2017
(Circular) (Six-base) MAPSORT of: pFN21AE2017.seq Check: 8049 from: 1 to: 6487
pFN21AE2017 6487 bp
With 182 enzymes: SgfI *
August 16, 2010 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2086 2262 2086
Size: 176 6311
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4297 278
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 3830 1937
Size: 1677 216 4594
Fragments arranged by size:
4594 1677 216
Acc65I G'GTAC_C
Cuts at: 3808 3808
Size: 6487
AclI AA'CG_TT
Cuts at: 1563 4614 4987 1563
Size: 3051 373 3063
Fragments arranged by size:
3063 3051 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2081 2229 3041 3372 3741 4550 5934 6202
Size: 449 148 812 331 369 809 1384 268
Cuts at: 6202 1632
Size: 1917
Fragments arranged by size:
1917 1384 812 809 449 369 331 268
148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6290
AflIII A'CryG_T
Cuts at: 1203 5386 6363 1203
Size: 4183 977 1327
Fragments arranged by size:
4183 1327 977
AgeI A'CCGG_T
Cuts at: 5318 5318
Size: 6487
AhdI GACnn_n'nnGTC
Cuts at: 5216 5216
Size: 6487
AleI CACnn'nnGTG
Cuts at: 1166 3663 1166
Size: 2497 3990
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3145 1184
Size: 1961 4526
AlwNI CAG_nnn'CTG
Cuts at: 4230 5802 4230
Size: 1572 4915
ApaLI G'TGCA_C
Cuts at: 2919 4544 5700 2919
Size: 1625 1156 3706
Fragments arranged by size:
3706 1625 1156
ApoI r'AATT_y
Cuts at: 1493 2126 3686 3796 4022 4174 6283 1493
Size: 633 1560 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 1560 633 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 5041 160
Size: 872 4009 1606
Fragments arranged by size:
4009 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
AvaI C'yCGr_G
Cuts at: 1870 1943 3812 1870
Size: 73 1869 4545
Fragments arranged by size:
4545 1869 73
BamHI G'GATC_C
Cuts at: 2293 3817 4184 2293
Size: 1524 367 4596
Fragments arranged by size:
4596 1524 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2306 3166 3808 5263 618
Size: 325 217 627 519 860 642 1455 1842
Fragments arranged by size:
1842 1455 860 642 627 519 325 217
BanII G_rGCy'C
Cuts at: 1391 3019 3806 1391
Size: 1628 787 4072
Fragments arranged by size:
4072 1628 787
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3029 3378 3501 928
Size: 971 1130 349 123 3914
Fragments arranged by size:
3914 1130 971 349 123
BbvCI CC'TCA_GC
Cuts at: 2361 2361
Size: 6487
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3135 3169 4678 4712 1903
Size: 34 1198 34 1509 34 3678
Fragments arranged by size:
3678 1509 1198 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2976 4379 5589 2976
Size: 1403 1210 3874
Fragments arranged by size:
3874 1403 1210
BclI T'GATC_A
Cuts at: 1548 2505 1548
Size: 957 5530
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5098 136
Size: 107 122 71 631 605 3426 1525
Fragments arranged by size:
3426 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6482 6482
Size: 6487
BlpI GC'TnA_GC
Cuts at: 3904 3904
Size: 6487
Bme1580I G_kGCm'C
Cuts at: 1790 2923 3171 4548 5704 1790
Size: 1133 248 1377 1156 2573
Fragments arranged by size:
2573 1377 1156 1133 248
BmgBI CAC'GTC
Cuts at: 1318 2924 1318
Size: 1606 4881
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2641 4218 5176
Size: 416 53 83 399 24 1224 1577 958
Cuts at: 5176 442
Size: 1753
Fragments arranged by size:
1753 1577 1224 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6487
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 6487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2367 2708 2717 2791 3255 3341 3393 5147
Size: 1128 341 9 74 464 86 52 1754
Cuts at: 5147 1239
Size: 2579
Fragments arranged by size:
2579 1754 1128 464 341 86 74 52
9
Bpu10I CC'TnA_GC
Cuts at: 2361 2727 2361
Size: 366 6121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4612 5477 5775 4612
Size: 865 298 5324
Fragments arranged by size:
5324 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2240 3569 5150 882
Size: 617 741 1329 1581 2219
Fragments arranged by size:
2219 1581 1329 741 617
BsaAI yAC'GTr
Cuts at: 493 1204 2020 4278 493
Size: 711 816 2258 2702
Fragments arranged by size:
2702 2258 816 711
BsaBI GATnn'nnATC
Cuts at: 2792 6481 2792
Size: 3689 2798
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4294 4676 5311 275
Size: 53 83 186 936 2761 382 635 1451
Fragments arranged by size:
2761 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2596 4920 5318 5592 5739 6150 2596
Size: 2324 398 274 147 411 2933
Fragments arranged by size:
2933 2324 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2846 2876 1178
Size: 30 165 30 912 30 501 30 4789
Fragments arranged by size:
4789 912 501 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2087 2117 2336 2364 2388 2714 3428 3437
Size: 901 30 219 28 24 326 714 9
Cuts at: 3437 3563 3662 3713 1186
Size: 126 99 51 3960
Fragments arranged by size:
3960 901 714 326 219 126 99 51
30 28 24 9
BseYI C'CCAG_C
Cuts at: 2440 2446 2885 5690 6186 2440
Size: 6 439 2805 496 2741
Fragments arranged by size:
2805 2741 496 439 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 3002 3354 1163
Size: 573 633 633 352 4296
Fragments arranged by size:
4296 633 633 573 352
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 3937 4698 5726 1540
Size: 275 186 39 1897 761 1028 2301
Fragments arranged by size:
2301 1897 1028 761 275 186 39
BsiHKAI G_wGCw'C
Cuts at: 2034 2115 2923 4548 4633 5704 2034
Size: 81 808 1625 85 1071 2817
Fragments arranged by size:
2817 1625 1071 808 85 81
BsmI GAATG_Cn'
Cuts at: 3998 4091 3998
Size: 93 6394
BsmBI CGTCTCn'nnnn_
Cuts at: 2930 3419 2930
Size: 489 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2115 2923 3019 3171 3806 4548
Size: 399 244 81 808 96 152 635 742
Cuts at: 4548 4633 5704 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 635 399 244 152
96 85 81
BspEI T'CCGG_A
Cuts at: 2596 6150 2596
Size: 3554 2933
BspHI T'CATG_A
Cuts at: 2221 3264 4376 6106 2221
Size: 1043 1112 1730 2602
Fragments arranged by size:
2602 1730 1112 1043
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2086 2262 2865 3842 844
Size: 1242 176 603 977 3489
Fragments arranged by size:
3489 1242 977 603 176
BsrBI CCG'CTC
Cuts at: 1394 1823 4374 1394
Size: 429 2551 3507
Fragments arranged by size:
3507 2551 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3282 4982 5156 65
Size: 1183 2034 1700 174 1396
Fragments arranged by size:
2034 1700 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3703 5131 5318 1700
Size: 110 1893 1428 187 2869
Fragments arranged by size:
2869 1893 1428 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6487
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6487
BssSI C'ACGA_G
Cuts at: 3172 4547 5559 3172
Size: 1375 1012 4100
Fragments arranged by size:
4100 1375 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6487
BstBI TT'CG_AA
Cuts at: 5291 5291
Size: 6487
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6487
BstXI CCAn_nnnn'nTGG
Cuts at: 2654 2764 2815 3665 2654
Size: 110 51 850 5476
Fragments arranged by size:
5476 850 110 51
BstYI r'GATC_y
Cuts at: 1972 2293 2985 3817 4184 4569 4586 6038 6124
Size: 321 692 832 367 385 17 1452 86
Cuts at: 6124 6136 6482 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 832 692 385 367 346 321
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3477 3645 3477
Size: 168 6319
BtgI C'CryG_G
Cuts at: 513 1065 2004 2757 513
Size: 552 939 753 4243
Fragments arranged by size:
4243 939 753 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2914 487
Size: 683 56 1688 4060
Fragments arranged by size:
4060 1688 683 56
BtsI GCAGTG_nn'
Cuts at: 3718 4003 4797 4817 3718
Size: 285 794 20 5388
Fragments arranged by size:
5388 794 285 20
ClaI AT'CG_AT
Cuts at: 1554 3150 1554
Size: 1596 4891
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3055 3090 529
Size: 35 2491 35 3926
Fragments arranged by size:
3926 2491 35 35
DraI TTT'AAA
Cuts at: 3791 3791
Size: 6487
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2419 5494 809
Size: 507 1103 3075 1802
Fragments arranged by size:
3075 1802 1103 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2958 3076 3934
Size: 54 1425 50 275 18 1128 118 858
Cuts at: 3934 4823 6452 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1128 889 858 275 118 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3934 1537
Size: 275 2122 4090
Fragments arranged by size:
4090 2122 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3204 3567 3717 4417 1353
Size: 1851 363 150 700 3423
Fragments arranged by size:
3423 1851 700 363 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2222 5074 5448 5594 387
Size: 1064 138 633 2852 374 146 1280
Fragments arranged by size:
2852 1280 1064 633 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2229 2367 2708 2717 2791 3041
Size: 393 449 148 138 341 9 74 250
Cuts at: 3041 3255 3341 3372 3393 3741 4550 5147 5934
Size: 214 86 31 21 348 809 597 787
Cuts at: 5934 6202 1239
Size: 268 1524
Fragments arranged by size:
1524 809 787 597 449 393 348 341
268 250 214 148 138 86 74 31
21 9
EcoO109I rG'GnC_Cy
Cuts at: 2227 2365 3004 2227
Size: 138 639 5710
Fragments arranged by size:
5710 639 138
EcoRI G'AATT_C
Cuts at: 3796 4174 3796
Size: 378 6109
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3358 3358
Size: 6487
FspI TGC'GCA
Cuts at: 4993 4993
Size: 6487
HaeII r_GCGC'y
Cuts at: 1678 2060 5634 1678
Size: 382 3574 2531
Fragments arranged by size:
3574 2531 382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3143 3175 3203 3235 3550
Size: 32 135 32 1571 32 28 32 315
Cuts at: 3550 3582 5128 5160 5202 5234 1373
Size: 32 1546 32 42 32 2626
Fragments arranged by size:
2626 1571 1546 315 135 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 3831 4077 669
Size: 988 281 1893 246 3079
Fragments arranged by size:
3079 1893 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3191 748
Size: 2443 4044
HpaI GTT'AAC
Cuts at: 4077 4077
Size: 6487
KpnI G_GTAC'C
Cuts at: 3812 3812
Size: 6487
MfeI C'AATT_G
Cuts at: 4086 4086
Size: 6487
MluI A'CGCG_T
Cuts at: 6363 6363
Size: 6487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5601 5785 1305
Size: 4296 184 2007
Fragments arranged by size:
4296 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 10
Size: 54 1425 343 1128 3537
Fragments arranged by size:
3537 1425 1128 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2762 3663 4445 4804 4963 518
Size: 648 1596 901 782 359 159 2042
Fragments arranged by size:
2042 1596 901 782 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2440 2536 3086 3581 3903 4580 5728 5973
Size: 834 96 550 495 322 677 1148 245
Cuts at: 5973 1606
Size: 2120
Fragments arranged by size:
2120 1148 834 677 550 495 322 245
96
NcoI C'CATG_G
Cuts at: 513 1065 2004 2757 513
Size: 552 939 753 4243
Fragments arranged by size:
4243 939 753 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6487
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6487
NotI GC'GGCC_GC
Cuts at: 3934 3934
Size: 6487
NspI r_CATG'y
Cuts at: 1132 1229 2939 3845 5390 1132
Size: 97 1710 906 1545 2229
Fragments arranged by size:
2229 1710 1545 906 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6487
PciI A'CATG_T
Cuts at: 5386 5386
Size: 6487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 3047 1108
Size: 1290 649 4548
Fragments arranged by size:
4548 1290 649
PfoI T'CCnGG_A
Cuts at: 2526 2526
Size: 6487
PmeI GTTT'AAAC
Cuts at: 3791 3791
Size: 6487
PmlI CAC'GTG
Cuts at: 2020 2020
Size: 6487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4556 4588 6069 6101 4556
Size: 32 1481 32 4942
Fragments arranged by size:
4942 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2365 2227
Size: 138 6349
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6487
PsiI TTA'TAA
Cuts at: 4057 4057
Size: 6487
PstI C_TGCA'G
Cuts at: 830 2091 3839 830
Size: 1261 1748 3478
Fragments arranged by size:
3478 1748 1261
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6487
PvuII CAG'CTG
Cuts at: 2440 2536 3086 2440
Size: 96 550 5841
Fragments arranged by size:
5841 550 96
SalI G'TCGA_C
Cuts at: 1936 3829 1936
Size: 1893 4594
SapI GCTCTTCn'nnn_
Cuts at: 3204 3204
Size: 6487
SbfI CC_TGCA'GG
Cuts at: 3839 3839
Size: 6487
ScaI AGT'ACT
Cuts at: 2277 3406 4735 6447 2277
Size: 1129 1329 1712 2317
Fragments arranged by size:
2317 1712 1329 1129
SfcI C'TryA_G
Cuts at: 826 1045 2087 3835 4970 5651 5842 826
Size: 219 1042 1748 1135 681 191 1471
Fragments arranged by size:
1748 1471 1135 1042 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6487
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6487
SmaI CCC'GGG
Cuts at: 1872 3814 1872
Size: 1942 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4591 5492 5754 820
Size: 197 926 2648 901 262 1553
Fragments arranged by size:
2648 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4278 493
Size: 3785 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6487
SphI G_CATG'C
Cuts at: 1132 3845 1132
Size: 2713 3774
SspI AAT'ATT
Cuts at: 5 52 4411 5
Size: 47 4359 2081
Fragments arranged by size:
4359 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6487
StyI C'CwwG_G
Cuts at: 513 1065 2004 2184 2721 2757 2989 3543 3621
Size: 552 939 180 537 36 232 554 78
Cuts at: 3621 3926 513
Size: 305 3074
Fragments arranged by size:
3074 939 554 552 537 305 232 180
78 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2429 3634 4514 4531 4684 4869 5314
Size: 1255 849 1205 880 17 153 185 445
Cuts at: 5314 325
Size: 1498
Fragments arranged by size:
1498 1255 1205 880 849 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2275 3404 4733
Size: 274 80 33 51 1443 298 1129 1329
Cuts at: 4733 6445 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1329 1129 298 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2444 2476 3580 3612 6447 6479 2243
Size: 32 169 32 1104 32 2835 32 2251
Fragments arranged by size:
2835 2251 1104 169 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6487
XbaI T'CTAG_A
Cuts at: 3823 3823
Size: 6487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2524 2524
Size: 6487
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6487
XmaI C'CCGG_G
Cuts at: 1870 3812 1870
Size: 1942 4545
XmnI GAAnn'nnTTC
Cuts at: 3761 4616 3761
Size: 855 5632
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4295 276
Size: 53 83 186 936 2761 2468
Fragments arranged by size:
2761 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI