Restriction map for
pFN21AE2012
(Circular) (Six-base) MAPSORT of: pFN21AE2012.seq Check: 4494 from: 1 to: 6376
pFN21AE2012 6376 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2042 2355 3719 1937
Size: 105 313 1364 4594
Fragments arranged by size:
4594 1364 313 105
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 4503 4876 1563
Size: 2940 373 3063
Fragments arranged by size:
3063 2940 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4439 5823 6091 1632
Size: 2807 1384 268 1917
Fragments arranged by size:
2807 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 3312 5275 6252 1203
Size: 2109 1963 977 1327
Fragments arranged by size:
2109 1963 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 2038 5105 2038
Size: 3067 3309
AleI CACnn'nnGTG
Cuts at: 1166 3325 1166
Size: 2159 4217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2819 2851 2819
Size: 32 6344
AlwNI CAG_nnn'CTG
Cuts at: 4119 5691 4119
Size: 1572 4804
ApaI G_GGCC'C
Cuts at: 2533 2592 2883 2533
Size: 59 291 6026
Fragments arranged by size:
6026 291 59
ApaLI G'TGCA_C
Cuts at: 3231 3476 4433 5589 3231
Size: 245 957 1156 4018
Fragments arranged by size:
4018 1156 957 245
ApoI r'AATT_y
Cuts at: 1493 3685 3911 4063 6172 1493
Size: 2192 226 152 2109 1697
Fragments arranged by size:
2192 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 2331 2891 3038 3342 3569 3701 1870
Size: 73 388 560 147 304 227 132 4545
Fragments arranged by size:
4545 560 388 304 227 147 132 73
BamHI G'GATC_C
Cuts at: 3077 3706 4073 3077
Size: 629 367 5380
Fragments arranged by size:
5380 629 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3033 3210 3614 3697 5152
Size: 325 217 627 1246 177 404 83 1455
Cuts at: 5152 618
Size: 1842
Fragments arranged by size:
1842 1455 1246 627 404 325 217 177
83
BanII G_rGCy'C
Cuts at: 1391 2533 2540 2592 2684 2883 3695 1391
Size: 1142 7 52 92 199 812 4072
Fragments arranged by size:
4072 1142 812 199 92 52 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2858 3032 928
Size: 971 959 174 4272
Fragments arranged by size:
4272 971 959 174
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3427 3461 4567 4601 1903
Size: 34 1490 34 1106 34 3678
Fragments arranged by size:
3678 1490 1106 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2027 4987 136
Size: 107 122 71 631 605 355 2960 1525
Fragments arranged by size:
2960 1525 631 605 355 122 107 71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 2385 3793 2385
Size: 1408 4968
Bme1580I G_kGCm'C
Cuts at: 1790 2533 2592 2691 2883 3235 3480 3617 4437
Size: 743 59 99 192 352 245 137 820
Cuts at: 4437 5593 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 820 743 352 245 192 137
99 59
BmgBI CAC'GTC
Cuts at: 1318 2353 2412 2641 3030 3207 1318
Size: 1035 59 229 389 177 4487
Fragments arranged by size:
4487 1035 389 229 177 59
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 4107 5065
Size: 416 53 83 399 24 1226 1464 958
Cuts at: 5065 442
Size: 1753
Fragments arranged by size:
1753 1464 1226 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2524 2524
Size: 6376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2257 2654 2696 3374 5036 1239
Size: 1018 397 42 678 1662 2579
Fragments arranged by size:
2579 1662 1018 678 397 42
Bpu10I CC'TnA_GC
Cuts at: 2534 2610 2534
Size: 76 6300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2494 3581 3647 4501 5366 5664 2494
Size: 1087 66 854 865 298 3206
Fragments arranged by size:
3206 1087 865 854 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2081 5039 882
Size: 617 582 2958 2219
Fragments arranged by size:
2958 2219 617 582
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 6370 6370
Size: 6376
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2019 4183 4565 5200
Size: 53 83 186 936 486 2164 382 635
Cuts at: 5200 275
Size: 1451
Fragments arranged by size:
2164 1451 936 635 486 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3201 4809 5207 5481 5628 6039 3201
Size: 1608 398 274 147 411 3538
Fragments arranged by size:
3538 1608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3125 3155 1178
Size: 30 165 30 1722 30 4399
Fragments arranged by size:
4399 1722 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2357 2360 2773 3083 1186
Size: 913 258 3 413 310 4479
Fragments arranged by size:
4479 913 413 310 258 3
BseYI C'CCAG_C
Cuts at: 2216 2585 2690 2914 2944 5579 6075 2216
Size: 369 105 224 30 2635 496 2517
Fragments arranged by size:
2635 2517 496 369 224 105 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3037 1163
Size: 573 1301 4502
Fragments arranged by size:
4502 1301 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2443 3535 3826 4587 5615 1540
Size: 275 186 442 1092 291 761 1028 2301
Fragments arranged by size:
2301 1092 1028 761 442 291 275 186
BsiHKAI G_wGCw'C
Cuts at: 2330 2352 2411 2684 3045 3235 3453 3480 3576
Size: 22 59 273 361 190 218 27 96
Cuts at: 3576 4437 4522 5593 2330
Size: 861 85 1071 3113
Fragments arranged by size:
3113 1071 861 361 273 218 190 96
85 59 27 22
BsmI GAATG_Cn'
Cuts at: 3419 3887 3980 3419
Size: 468 93 5815
Fragments arranged by size:
5815 468 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2352 2411 2533 2540 2592 2684
Size: 399 540 22 59 122 7 52 92
Cuts at: 2684 2691 2883 3045 3235 3453 3480 3576 3617
Size: 7 192 162 190 218 27 96 41
Cuts at: 3617 3695 4437 4522 5593 1391
Size: 78 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 218 192 190
162 122 96 92 85 78 59 52
41 27 22 7 7
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2995 3731 844
Size: 2151 736 3489
Fragments arranged by size:
3489 2151 736
BsrBI CCG'CTC
Cuts at: 1394 1823 3097 3490 4263 1394
Size: 429 1274 393 773 3507
Fragments arranged by size:
3507 1274 773 429 393
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3530 5020 5207 1700
Size: 110 1720 1490 187 2869
Fragments arranged by size:
2869 1720 1490 187 110
BsrGI T'GTAC_A
Cuts at: 96 2467 96
Size: 2371 4005
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 3269 3485 4436 5448 3269
Size: 216 951 1012 4197
Fragments arranged by size:
4197 1012 951 216
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3060 794
Size: 2266 4110
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstXI CCAn_nnnn'nTGG
Cuts at: 2800 2800
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 3077 3706 4073 4458 4475 5927 6013 6025
Size: 1105 629 367 385 17 1452 86 12
Cuts at: 6025 6371 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1105 629 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2304 2304
Size: 6376
BtgI C'CryG_G
Cuts at: 513 1065 2004 3018 3549 513
Size: 552 939 1014 531 3340
Fragments arranged by size:
3340 1014 939 552 531
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2980 487
Size: 683 56 1754 3883
Fragments arranged by size:
3883 1754 683 56
BtsI GCAGTG_nn'
Cuts at: 3058 3892 4686 4706 3058
Size: 834 794 20 4728
Fragments arranged by size:
4728 834 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6341
DraI TTT'AAA
Cuts at: 3680 3680
Size: 6376
DraIII CAC_nnn'GTG
Cuts at: 3239 3384 3239
Size: 145 6231
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3532 3552 3823
Size: 54 1425 50 275 18 1702 20 271
Cuts at: 3823 4712 6341 8
Size: 889 1629 43
Fragments arranged by size:
1702 1629 1425 889 275 271 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 3823 1537
Size: 275 1720 291 4090
Fragments arranged by size:
4090 1720 291 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3143 3674 4306 1353
Size: 1790 531 632 3423
Fragments arranged by size:
3423 1790 632 531
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 4963 5337 5483 387
Size: 1064 138 456 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 456 374 146 138
EcoICRI GAG'CTC
Cuts at: 2682 2682
Size: 6376
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2257 2654 2696 3374 4439 5036 5823
Size: 393 625 397 42 678 1065 597 787
Cuts at: 5823 6091 1239
Size: 268 1524
Fragments arranged by size:
1524 1065 787 678 625 597 397 393
268 42
EcoO109I rG'GnC_Cy
Cuts at: 2173 2529 2530 2589 2629 2879 2880 2979 2173
Size: 356 1 59 40 250 1 99 5570
Fragments arranged by size:
5570 356 250 99 59 40 1 1
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3322 3634 3322
Size: 312 6064
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 5523 1678
Size: 3845 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 3062 3094 5017
Size: 32 135 32 452 32 1006 32 1923
Cuts at: 5017 5049 5091 5123 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1923 1006 452 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3720 3966 669
Size: 988 281 1782 246 3079
Fragments arranged by size:
3079 1782 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6376
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3975 3975
Size: 6376
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2061 2322 2805 5490 5674 1305
Size: 756 261 483 2685 184 2007
Fragments arranged by size:
2685 2007 756 483 261 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3554 10
Size: 54 1425 343 1722 2832
Fragments arranged by size:
2832 1722 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2711 3325 3548 4334 4693 4852
Size: 648 1236 309 614 223 786 359 159
Cuts at: 4852 518
Size: 2042
Fragments arranged by size:
2042 1236 786 648 614 359 309 223
159
MspA1I CmG'CkG
Cuts at: 1606 2220 2384 2902 3537 3792 4469 5617 5862
Size: 614 164 518 635 255 677 1148 245
Cuts at: 5862 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 635 614 518 255 245
164
NcoI C'CATG_G
Cuts at: 513 1065 2004 3018 3549 513
Size: 552 939 1014 531 3340
Fragments arranged by size:
3340 1014 939 552 531
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6376
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 2914 3316 3734 5279 1132
Size: 97 1685 402 418 1545 2229
Fragments arranged by size:
2229 1685 1545 418 402 97
PasI CC'CwG_GG
Cuts at: 1348 2416 2845 2873 1348
Size: 1068 429 28 4851
Fragments arranged by size:
4851 1068 429 28
PciI A'CATG_T
Cuts at: 3312 5275 3312
Size: 1963 4413
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6376
PfoI T'CCnGG_A
Cuts at: 3080 3174 3351 3080
Size: 94 177 6105
Fragments arranged by size:
6105 177 94
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3350 3382 4445 4477 5958 5990 3350
Size: 32 1063 32 1481 32 3736
Fragments arranged by size:
3736 1481 1063 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 3946 3946
Size: 6376
PspOMI G'GGCC_C
Cuts at: 2529 2588 2879 2529
Size: 59 291 6026
Fragments arranged by size:
6026 291 59
PstI C_TGCA'G
Cuts at: 830 2497 2928 3728 830
Size: 1667 431 800 3478
Fragments arranged by size:
3478 1667 800 431
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
PvuII CAG'CTG
Cuts at: 2384 2902 2384
Size: 518 5858
SacI G_AGCT'C
Cuts at: 2684 2684
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 3718 1936
Size: 1782 4594
SapI GCTCTTCn'nnn_
Cuts at: 3674 3674
Size: 6376
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2356 2493 2924 3724 4859 5540 5731
Size: 219 1311 137 431 800 1135 681 191
Cuts at: 5731 826
Size: 1471
Fragments arranged by size:
1471 1311 1135 800 681 431 219 191
137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 2893 3344 3703 1872
Size: 1021 451 359 4545
Fragments arranged by size:
4545 1021 451 359
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2509 3596 3662 4480 5381 5643
Size: 197 926 566 1087 66 818 901 262
Cuts at: 5643 820
Size: 1553
Fragments arranged by size:
1553 1087 926 901 818 566 262 197
66
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6376
SphI G_CATG'C
Cuts at: 1132 2914 3734 1132
Size: 1782 820 3774
Fragments arranged by size:
3774 1782 820
SrfI GCCC'GGGC
Cuts at: 3344 3344
Size: 6376
SspI AAT'ATT
Cuts at: 5 52 4300 5
Size: 47 4248 2081
Fragments arranged by size:
4248 2081 47
StuI AGG'CCT
Cuts at: 1517 3287 1517
Size: 1770 4606
StyI C'CwwG_G
Cuts at: 513 1065 2004 2571 3018 3549 3815 513
Size: 552 939 567 447 531 266 3074
Fragments arranged by size:
3074 939 567 552 531 447 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2327 2410 2771 3071 3337 4403 4420
Size: 1255 747 83 361 300 266 1066 17
Cuts at: 4420 4573 4758 5203 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1066 747 445 361 300 266
185 153 83 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2467 4622 6334
Size: 274 80 33 51 1443 490 2155 1712
Cuts at: 6334 96
Size: 138
Fragments arranged by size:
2155 1712 1443 490 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2133 2165 6336 6368 2133
Size: 32 4171 32 2141
Fragments arranged by size:
4171 2141 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2797 2797
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 2891 3342 3701 1870
Size: 1021 451 359 4545
Fragments arranged by size:
4545 1021 451 359
XmnI GAAnn'nnTTC
Cuts at: 3089 3656 4505 3089
Size: 567 849 4960
Fragments arranged by size:
4960 849 567
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SwaI