Restriction map for
pFN21AE2008
(Circular) (Six-base) MAPSORT of: pFN21AE2008.seq Check: 847 from: 1 to: 6454
pFN21AE2008 6454 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2669 2737 2669
Size: 68 6386
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4264 278
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2090 3642 3797 1937
Size: 153 1552 155 4594
Fragments arranged by size:
4594 1552 155 153
Acc65I G'GTAC_C
Cuts at: 3775 3775
Size: 6454
AclI AA'CG_TT
Cuts at: 1563 4581 4954 1563
Size: 3018 373 3063
Fragments arranged by size:
3063 3018 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2053 2197 3456 3768 4517 5901 6169 1632
Size: 421 144 1259 312 749 1384 268 1917
Fragments arranged by size:
1917 1384 1259 749 421 312 268 144
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6257
AflIII A'CryG_T
Cuts at: 1203 2364 3682 5353 6330 1203
Size: 1161 1318 1671 977 1327
Fragments arranged by size:
1671 1327 1318 1161 977
AgeI A'CCGG_T
Cuts at: 5285 5285
Size: 6454
AhdI GACnn_n'nnGTC
Cuts at: 5183 5183
Size: 6454
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6454
AlwNI CAG_nnn'CTG
Cuts at: 2028 4197 5769 2028
Size: 2169 1572 2713
Fragments arranged by size:
2713 2169 1572
ApaLI G'TGCA_C
Cuts at: 4511 5667 4511
Size: 1156 5298
ApoI r'AATT_y
Cuts at: 1493 2098 2843 3763 3989 4141 6250 1493
Size: 605 745 920 226 152 2109 1697
Fragments arranged by size:
2109 1697 920 745 605 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3399 5008 160
Size: 872 2367 1609 1606
Fragments arranged by size:
2367 1609 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
AvaI C'yCGr_G
Cuts at: 1870 1943 3779 1870
Size: 73 1836 4545
Fragments arranged by size:
4545 1836 73
BamHI G'GATC_C
Cuts at: 3784 4151 3784
Size: 367 6087
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2374 3775 5230 618
Size: 325 217 627 587 1401 1455 1842
Fragments arranged by size:
1842 1455 1401 627 587 325 217
BanII G_rGCy'C
Cuts at: 1391 3773 1391
Size: 2382 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2059 2490 3140 928
Size: 971 160 431 650 4242
Fragments arranged by size:
4242 971 650 431 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4645 4679 1903
Size: 34 2708 34 3678
Fragments arranged by size:
3678 2708 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4346 5556 4346
Size: 1210 5244
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5065 136
Size: 107 122 71 631 605 3393 1525
Fragments arranged by size:
3393 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6449 6449
Size: 6454
BlpI GC'TnA_GC
Cuts at: 3871 3871
Size: 6454
Bme1580I G_kGCm'C
Cuts at: 1790 4515 5671 1790
Size: 2725 1156 2573
Fragments arranged by size:
2725 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2192 2579 3276
Size: 416 53 83 399 24 775 387 697
Cuts at: 3276 4185 5143 442
Size: 909 958 1753
Fragments arranged by size:
1753 958 909 775 697 416 399 387
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3081 1056
Size: 2025 4429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 3497 5114 1239
Size: 1400 858 1617 2579
Fragments arranged by size:
2579 1617 1400 858
Bpu10I CC'TnA_GC
Cuts at: 2815 2815
Size: 6454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4579 5444 5742 4579
Size: 865 298 5291
Fragments arranged by size:
5291 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3614 5117 882
Size: 617 2115 1503 2219
Fragments arranged by size:
2219 2115 1503 617
BsaAI yAC'GTr
Cuts at: 493 1204 4245 493
Size: 711 3041 2702
Fragments arranged by size:
3041 2702 711
BsaBI GATnn'nnATC
Cuts at: 6448 6448
Size: 6454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4261 4643 5278 275
Size: 53 83 186 936 2728 382 635 1451
Fragments arranged by size:
2728 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4887 5285 5559 5706 6117 4887
Size: 398 274 147 411 5224
Fragments arranged by size:
5224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2541 2571 1178
Size: 30 165 30 1138 30 5061
Fragments arranged by size:
5061 1138 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3602 1186
Size: 2416 4038
BseYI C'CCAG_C
Cuts at: 5657 6153 5657
Size: 496 5958
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 1163
Size: 573 522 5359
Fragments arranged by size:
5359 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4665 5693 1540
Size: 275 186 1903 761 1028 2301
Fragments arranged by size:
2301 1903 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4515 4600 5671 4515
Size: 85 1071 5298
Fragments arranged by size:
5298 1071 85
BsmI GAATG_Cn'
Cuts at: 3091 3965 4058 3091
Size: 874 93 5487
Fragments arranged by size:
5487 874 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3773 4515 4600 5671 1391
Size: 399 1983 742 85 1071 2174
Fragments arranged by size:
2174 1983 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6117 6117
Size: 6454
BspHI T'CATG_A
Cuts at: 4343 6073 4343
Size: 1730 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2669 2737 3396 3809 844
Size: 1825 68 659 413 3489
Fragments arranged by size:
3489 1825 659 413 68
BsrBI CCG'CTC
Cuts at: 1394 1823 2552 4341 1394
Size: 429 729 1789 3507
Fragments arranged by size:
3507 1789 729 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2926 4949 5123 65
Size: 1183 1678 2023 174 1396
Fragments arranged by size:
2023 1678 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5098 5285 1700
Size: 110 3288 187 2869
Fragments arranged by size:
3288 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3651 96
Size: 3555 2899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6454
BssSI C'ACGA_G
Cuts at: 4514 5526 4514
Size: 1012 5442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6454
BstBI TT'CG_AA
Cuts at: 5258 5258
Size: 6454
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6454
BstYI r'GATC_y
Cuts at: 1972 2216 3784 4151 4536 4553 6005 6091 6103
Size: 244 1568 367 385 17 1452 86 12
Cuts at: 6103 6449 1972
Size: 346 1977
Fragments arranged by size:
1977 1568 1452 385 367 346 244 86
17 12
BstZ17I GTA'TAC
Cuts at: 2091 2091
Size: 6454
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4963
Fragments arranged by size:
4963 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5715
Fragments arranged by size:
5715 683 56
BtsI GCAGTG_nn'
Cuts at: 3364 3970 4764 4784 3364
Size: 606 794 20 5034
Fragments arranged by size:
5034 794 606 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6454
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6419
DraI TTT'AAA
Cuts at: 2569 3758 2569
Size: 1189 5265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5461 809
Size: 507 4145 1802
Fragments arranged by size:
4145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2689 3901 4790
Size: 54 1425 50 275 18 859 1212 889
Cuts at: 4790 6419 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1212 889 859 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3901 1537
Size: 275 2089 4090
Fragments arranged by size:
4090 2089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 3432 4384 1353
Size: 692 1387 952 3423
Fragments arranged by size:
3423 1387 952 692
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2047 5041 5415 5561 387
Size: 1064 138 458 2994 374 146 1280
Fragments arranged by size:
2994 1280 1064 458 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2197 2639 3456 3497 3768 4517
Size: 393 421 144 442 817 41 271 749
Cuts at: 4517 5114 5901 6169 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 817 787 749 597 442 421 393
271 268 144 41
EcoO109I rG'GnC_Cy
Cuts at: 3694 3694
Size: 6454
EcoRI G'AATT_C
Cuts at: 3763 4141 3763
Size: 378 6076
FspI TGC'GCA
Cuts at: 4960 4960
Size: 6454
HaeII r_GCGC'y
Cuts at: 1678 5601 1678
Size: 3923 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2262 2294 2541 2573 5095
Size: 32 135 32 690 32 247 32 2522
Cuts at: 5095 5127 5169 5201 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2522 690 247 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3798 4044 669
Size: 988 281 1860 246 3079
Fragments arranged by size:
3079 1860 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6454
HpaI GTT'AAC
Cuts at: 4044 4044
Size: 6454
KpnI G_GTAC'C
Cuts at: 3779 3779
Size: 6454
MfeI C'AATT_G
Cuts at: 4053 4053
Size: 6454
MluI A'CGCG_T
Cuts at: 6330 6330
Size: 6454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5568 5752 1305
Size: 4263 184 2007
Fragments arranged by size:
4263 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2691 10
Size: 54 1425 343 859 3773
Fragments arranged by size:
3773 1425 859 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 4412 4771 4930 518
Size: 648 1482 1764 359 159 2042
Fragments arranged by size:
2042 1764 1482 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3870 4547 5695 5940 1606
Size: 2264 677 1148 245 2120
Fragments arranged by size:
2264 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4963
Fragments arranged by size:
4963 939 552
NdeI CA'TA_TG
Cuts at: 387 2107 387
Size: 1720 4734
NheI G'CTAG_C
Cuts at: 1052 3077 1052
Size: 2025 4429
NotI GC'GGCC_GC
Cuts at: 3901 3901
Size: 6454
NspI r_CATG'y
Cuts at: 1132 1229 2347 3686 3812 5357 1132
Size: 97 1118 1339 126 1545 2229
Fragments arranged by size:
2229 1545 1339 1118 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6454
PciI A'CATG_T
Cuts at: 3682 5353 3682
Size: 1671 4783
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2081 3342 1108
Size: 973 1261 4220
Fragments arranged by size:
4220 1261 973
PmeI GTTT'AAAC
Cuts at: 3758 3758
Size: 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 6036 6068 4523
Size: 32 1481 32 4909
Fragments arranged by size:
4909 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3694 3694
Size: 6454
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6454
PsiI TTA'TAA
Cuts at: 4024 4024
Size: 6454
PstI C_TGCA'G
Cuts at: 830 3195 3806 830
Size: 2365 611 3478
Fragments arranged by size:
3478 2365 611
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6454
SalI G'TCGA_C
Cuts at: 1936 3796 1936
Size: 1860 4594
SapI GCTCTTCn'nnn_
Cuts at: 2045 2045
Size: 6454
SbfI CC_TGCA'GG
Cuts at: 3806 3806
Size: 6454
ScaI AGT'ACT
Cuts at: 4702 6414 4702
Size: 1712 4742
SfcI C'TryA_G
Cuts at: 826 1045 2739 3191 3802 4937 5618 5809 826
Size: 219 1694 452 611 1135 681 191 1471
Fragments arranged by size:
1694 1471 1135 681 611 452 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6454
SmaI CCC'GGG
Cuts at: 1872 3781 1872
Size: 1909 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4558 5459 5721 820
Size: 197 926 2615 901 262 1553
Fragments arranged by size:
2615 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4245 493
Size: 3752 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6454
SphI G_CATG'C
Cuts at: 1132 2347 3812 1132
Size: 1215 1465 3774
Fragments arranged by size:
3774 1465 1215
SspI AAT'ATT
Cuts at: 5 52 4378 5
Size: 47 4326 2081
Fragments arranged by size:
4326 2081 47
StuI AGG'CCT
Cuts at: 1517 2079 1517
Size: 562 5892
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 2080 2634 3893 513
Size: 552 939 9 67 554 1259 3074
Fragments arranged by size:
3074 1259 939 554 552 67 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2456 4481 4498 4651 4836 5281 325
Size: 1255 876 2025 17 153 185 445 1498
Fragments arranged by size:
2025 1498 1255 876 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 3651 4700
Size: 274 80 33 51 1443 645 1029 1049
Cuts at: 4700 6412 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1049 1029 645 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6414 6446 6414
Size: 32 6422
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6454
XbaI T'CTAG_A
Cuts at: 3790 3790
Size: 6454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2082 3339 2082
Size: 1257 5197
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6454
XmaI C'CCGG_G
Cuts at: 1870 3779 1870
Size: 1909 4545
XmnI GAAnn'nnTTC
Cuts at: 3321 4583 3321
Size: 1262 5192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4262 276
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI
SfoI SrfI SwaI