Restriction map for
pFN21AE2007
(Circular) (Six-base) MAPSORT of: pFN21AE2007.seq Check: 6050 from: 1 to: 6454
pFN21AE2007 6454 bp
With 182 enzymes: SgfI *
August 16, 2010 14:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3055 3055
Size: 6454
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4264 278
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2548 3195 3797 1937
Size: 611 647 602 4594
Fragments arranged by size:
4594 647 611 602
Acc65I G'GTAC_C
Cuts at: 3775 3775
Size: 6454
AclI AA'CG_TT
Cuts at: 1563 4581 4954 1563
Size: 3018 373 3063
Fragments arranged by size:
3063 3018 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3440 4517 5901 6169 1632
Size: 1808 1077 1384 268 1917
Fragments arranged by size:
1917 1808 1384 1077 268
AflII C'TTAA_G
Cuts at: 820 1017 2047 820
Size: 197 1030 5227
Fragments arranged by size:
5227 1030 197
AflIII A'CryG_T
Cuts at: 1203 2551 5353 6330 1203
Size: 1348 2802 977 1327
Fragments arranged by size:
2802 1348 1327 977
AgeI A'CCGG_T
Cuts at: 5285 5285
Size: 6454
AhdI GACnn_n'nnGTC
Cuts at: 5183 5183
Size: 6454
AleI CACnn'nnGTG
Cuts at: 1166 2222 3457 3686 1166
Size: 1056 1235 229 3934
Fragments arranged by size:
3934 1235 1056 229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6454
AlwNI CAG_nnn'CTG
Cuts at: 4197 5769 4197
Size: 1572 4882
ApaLI G'TGCA_C
Cuts at: 4511 5667 4511
Size: 1156 5298
ApoI r'AATT_y
Cuts at: 1493 2533 2919 3105 3763 3989 4141 6250 1493
Size: 1040 386 186 658 226 152 2109 1697
Fragments arranged by size:
2109 1697 1040 658 386 226 186 152
AseI AT'TA_AT
Cuts at: 160 1032 5008 160
Size: 872 3976 1606
Fragments arranged by size:
3976 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
AvaI C'yCGr_G
Cuts at: 1870 1943 3779 1870
Size: 73 1836 4545
Fragments arranged by size:
4545 1836 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2180 2213 2180
Size: 33 6421
BamHI G'GATC_C
Cuts at: 3784 4151 3784
Size: 367 6087
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2174 3775 5230 618
Size: 325 217 627 387 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 387 325 217
BanII G_rGCy'C
Cuts at: 1391 3773 1391
Size: 2382 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2276 2956 928
Size: 971 235 142 680 4426
Fragments arranged by size:
4426 971 680 235 142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4645 4679 1903
Size: 34 2708 34 3678
Fragments arranged by size:
3678 2708 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3195 4346 5556 3195
Size: 1151 1210 4093
Fragments arranged by size:
4093 1210 1151
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6454
BfrBI ATG'CAT
Cuts at: 2166 3405 2166
Size: 1239 5215
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2459 5065 136
Size: 107 122 71 631 605 787 2606 1525
Fragments arranged by size:
2606 1525 787 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6449 6449
Size: 6454
BlpI GC'TnA_GC
Cuts at: 2104 3871 2104
Size: 1767 4687
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2207 4515 5671 1790
Size: 387 30 2308 1156 2573
Fragments arranged by size:
2573 2308 1156 387 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3056 4185 5143
Size: 416 53 83 399 24 1639 1129 958
Cuts at: 5143 442
Size: 1753
Fragments arranged by size:
1753 1639 1129 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2458 3600 5114 1239
Size: 1219 1142 1514 2579
Fragments arranged by size:
2579 1514 1219 1142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2061 2889 4579 5444 5742 2061
Size: 828 1690 865 298 2773
Fragments arranged by size:
2773 1690 865 828 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2567 2742 3045 3378 5117 882
Size: 617 1068 175 303 333 1739 2219
Fragments arranged by size:
2219 1739 1068 617 333 303 175
BsaAI yAC'GTr
Cuts at: 493 1204 3238 4245 493
Size: 711 2034 1007 2702
Fragments arranged by size:
2702 2034 1007 711
BsaBI GATnn'nnATC
Cuts at: 6448 6448
Size: 6454
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4261 4643 5278 275
Size: 53 83 186 936 2728 382 635 1451
Fragments arranged by size:
2728 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4887 5285 5559 5706 6117 4887
Size: 398 274 147 411 5224
Fragments arranged by size:
5224 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 5518
Fragments arranged by size:
5518 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6454
BseYI C'CCAG_C
Cuts at: 3417 5657 6153 3417
Size: 2240 496 3718
Fragments arranged by size:
3718 2240 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2904 3123 3555 1163
Size: 573 1168 219 432 4062
Fragments arranged by size:
4062 1168 573 432 219
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3904 4665 5693 1540
Size: 275 186 1903 761 1028 2301
Fragments arranged by size:
2301 1903 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3043 4515 4600 5671 3043
Size: 1472 85 1071 3826
Fragments arranged by size:
3826 1472 1071 85
BsmI GAATG_Cn'
Cuts at: 2817 3006 3965 4058 2817
Size: 189 959 93 5213
Fragments arranged by size:
5213 959 189 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2207 3043 3773 4515 4600 5671
Size: 399 387 30 836 730 742 85 1071
Cuts at: 5671 1391
Size: 2174
Fragments arranged by size:
2174 1071 836 742 730 399 387 85
30
BspEI T'CCGG_A
Cuts at: 6117 6117
Size: 6454
BspHI T'CATG_A
Cuts at: 4343 6073 4343
Size: 1730 4724
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3055 3527 3809 844
Size: 2211 472 282 3489
Fragments arranged by size:
3489 2211 472 282
BsrBI CCG'CTC
Cuts at: 1394 1823 4341 1394
Size: 429 2518 3507
Fragments arranged by size:
3507 2518 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2557 2797 2845 4949 5123 65
Size: 1183 1309 240 48 2104 174 1396
Fragments arranged by size:
2104 1396 1309 1183 240 174 48
BsrFI r'CCGG_y
Cuts at: 1700 1810 5098 5285 1700
Size: 110 3288 187 2869
Fragments arranged by size:
3288 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6454
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6454
BssSI C'ACGA_G
Cuts at: 4514 5526 4514
Size: 1012 5442
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2228 2891 794
Size: 1434 663 4357
Fragments arranged by size:
4357 1434 663
BstBI TT'CG_AA
Cuts at: 3010 5258 3010
Size: 2248 4206
BstEII G'GTnAC_C
Cuts at: 1184 2618 1184
Size: 1434 5020
BstYI r'GATC_y
Cuts at: 1972 2083 3784 4151 4536 4553 6005 6091 6103
Size: 111 1701 367 385 17 1452 86 12
Cuts at: 6103 6449 1972
Size: 346 1977
Fragments arranged by size:
1977 1701 1452 385 367 346 111 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2153 3059 513
Size: 552 1088 906 3908
Fragments arranged by size:
3908 1088 906 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3277 487
Size: 683 56 2051 3664
Fragments arranged by size:
3664 2051 683 56
BtsI GCAGTG_nn'
Cuts at: 2217 2229 3970 4764 4784 2217
Size: 12 1741 794 20 3887
Fragments arranged by size:
3887 1741 794 20 12
ClaI AT'CG_AT
Cuts at: 1554 3558 1554
Size: 2004 4450
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2725 2760 529
Size: 35 2161 35 4223
Fragments arranged by size:
4223 2161 35 35
DraI TTT'AAA
Cuts at: 2307 3758 2307
Size: 1451 5003
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5461 809
Size: 507 4145 1802
Fragments arranged by size:
4145 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3690 3901 4790
Size: 54 1425 50 275 18 1860 211 889
Cuts at: 4790 6419 8
Size: 1629 43
Fragments arranged by size:
1860 1629 1425 889 275 211 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3901 1537
Size: 275 2089 4090
Fragments arranged by size:
4090 2089 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4384 1353
Size: 3031 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5041 5415 5561 387
Size: 1064 138 3452 374 146 1280
Fragments arranged by size:
3452 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2458 3440 3600 4517 5114 5901 6169
Size: 393 826 982 160 917 597 787 268
Cuts at: 6169 1239
Size: 1524
Fragments arranged by size:
1524 982 917 826 787 597 393 268
160
EcoO109I rG'GnC_Cy
Cuts at: 3584 3584
Size: 6454
EcoRI G'AATT_C
Cuts at: 3763 4141 3763
Size: 378 6076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2411 2411
Size: 6454
FspI TGC'GCA
Cuts at: 4960 4960
Size: 6454
HaeII r_GCGC'y
Cuts at: 1678 5601 1678
Size: 3923 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5095 5127 5169 5201 1373
Size: 32 135 32 3523 32 42 32 2626
Fragments arranged by size:
3523 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2264 3798 4044 669
Size: 988 281 326 1534 246 3079
Fragments arranged by size:
3079 1534 988 326 281 246
HindIII A'AGCT_T
Cuts at: 748 2009 2245 748
Size: 1261 236 4957
Fragments arranged by size:
4957 1261 236
HpaI GTT'AAC
Cuts at: 4044 4044
Size: 6454
KpnI G_GTAC'C
Cuts at: 3779 3779
Size: 6454
MfeI C'AATT_G
Cuts at: 4053 4053
Size: 6454
MluI A'CGCG_T
Cuts at: 6330 6330
Size: 6454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3104 3412 5568 5752 1305
Size: 1799 308 2156 184 2007
Fragments arranged by size:
2156 2007 1799 308 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3692 10
Size: 54 1425 343 1860 2772
Fragments arranged by size:
2772 1860 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 3457 3686 4412 4771 4930 518
Size: 648 1056 1235 229 726 359 159 2042
Fragments arranged by size:
2042 1235 1056 726 648 359 229 159
MspA1I CmG'CkG
Cuts at: 1606 2436 3870 4547 5695 5940 1606
Size: 830 1434 677 1148 245 2120
Fragments arranged by size:
2120 1434 1148 830 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2153 3059 513
Size: 552 1088 906 3908
Fragments arranged by size:
3908 1088 906 552
NdeI CA'TA_TG
Cuts at: 387 2423 3544 387
Size: 2036 1121 3297
Fragments arranged by size:
3297 2036 1121
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6454
NotI GC'GGCC_GC
Cuts at: 3901 3901
Size: 6454
NsiI A_TGCA'T
Cuts at: 2168 3407 2168
Size: 1239 5215
NspI r_CATG'y
Cuts at: 1132 1229 2322 2555 2781 3405 3812 5357 1132
Size: 97 1093 233 226 624 407 1545 2229
Fragments arranged by size:
2229 1545 1093 624 407 233 226 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6454
PciI A'CATG_T
Cuts at: 2551 5353 2551
Size: 2802 3652
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2213 1108
Size: 1105 5349
PmeI GTTT'AAAC
Cuts at: 3758 3758
Size: 6454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4523 4555 6036 6068 4523
Size: 32 1481 32 4909
Fragments arranged by size:
4909 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3620 1613
Size: 2007 4447
PsiI TTA'TAA
Cuts at: 4024 4024
Size: 6454
PstI C_TGCA'G
Cuts at: 830 2224 3806 830
Size: 1394 1582 3478
Fragments arranged by size:
3478 1582 1394
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6454
PvuII CAG'CTG
Cuts at: 2436 2436
Size: 6454
SalI G'TCGA_C
Cuts at: 1936 3796 1936
Size: 1860 4594
SbfI CC_TGCA'GG
Cuts at: 3806 3806
Size: 6454
ScaI AGT'ACT
Cuts at: 4702 6414 4702
Size: 1712 4742
SfcI C'TryA_G
Cuts at: 826 1045 2220 3802 4937 5618 5809 826
Size: 219 1175 1582 1135 681 191 1471
Fragments arranged by size:
1582 1471 1175 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6454
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6454
SmaI CCC'GGG
Cuts at: 1872 3781 1872
Size: 1909 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2047 2076 2904 4558 5459 5721
Size: 197 926 104 29 828 1654 901 262
Cuts at: 5721 820
Size: 1553
Fragments arranged by size:
1654 1553 926 901 828 262 197 104
29
SnaBI TAC'GTA
Cuts at: 493 4245 493
Size: 3752 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6454
SphI G_CATG'C
Cuts at: 1132 2781 3812 1132
Size: 1649 1031 3774
Fragments arranged by size:
3774 1649 1031
SspI AAT'ATT
Cuts at: 5 52 4378 5
Size: 47 4326 2081
Fragments arranged by size:
4326 2081 47
StuI AGG'CCT
Cuts at: 1517 3730 1517
Size: 2213 4241
StyI C'CwwG_G
Cuts at: 513 1065 2153 2473 2782 3059 3893 513
Size: 552 1088 320 309 277 834 3074
Fragments arranged by size:
3074 1088 834 552 320 309 277
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4481 4498 4651 4836 5281 325
Size: 1255 2901 17 153 185 445 1498
Fragments arranged by size:
2901 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2591 3744 4700
Size: 274 80 33 51 1443 614 1153 956
Cuts at: 4700 6412 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1153 956 614 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 6414 6446 2084
Size: 32 4298 32 2092
Fragments arranged by size:
4298 2092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6454
XbaI T'CTAG_A
Cuts at: 3790 3790
Size: 6454
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6454
XmaI C'CCGG_G
Cuts at: 1870 3779 1870
Size: 1909 4545
XmnI GAAnn'nnTTC
Cuts at: 2283 4583 2283
Size: 2300 4154
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4262 276
Size: 53 83 186 936 2728 2468
Fragments arranged by size:
2728 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI