Restriction map for
pFN21AE1995
(Circular) (Six-base) MAPSORT of: pFN21AE1995.seq Check: 4260 from: 1 to: 6430
pFN21AE1995 6430 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3252 3705 3252
Size: 453 5977
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4240 278
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3773 1937
Size: 1836 4594
Acc65I G'GTAC_C
Cuts at: 3751 3751
Size: 6430
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 2855 4493 5877 6145 1632
Size: 980 243 1638 1384 268 1917
Fragments arranged by size:
1917 1638 1384 980 268 243
AfeI AGC'GCT
Cuts at: 2222 3332 2222
Size: 1110 5320
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 2828 3349 1166
Size: 1662 521 4247
Fragments arranged by size:
4247 1662 521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 2887 3628 4173 5745 2887
Size: 741 545 1572 3572
Fragments arranged by size:
3572 1572 741 545
ApaI G_GGCC'C
Cuts at: 2292 2400 2686 2693 3152 2292
Size: 108 286 7 459 5570
Fragments arranged by size:
5570 459 286 108 7
ApaLI G'TGCA_C
Cuts at: 2791 2917 4487 5643 2791
Size: 126 1570 1156 3578
Fragments arranged by size:
3578 1570 1156 126
ApoI r'AATT_y
Cuts at: 1493 2890 3739 3965 4117 6226 1493
Size: 1397 849 226 152 2109 1697
Fragments arranged by size:
2109 1697 1397 849 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2118 2151 2393 2492 2568 2582 2686
Size: 73 175 33 242 99 76 14 104
Cuts at: 2686 3347 3525 3569 3654 3755 1870
Size: 661 178 44 85 101 4545
Fragments arranged by size:
4545 661 242 178 175 104 101 99
85 76 73 44 33 14
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2254 3356 3589 3751 5206
Size: 325 217 627 467 1102 233 162 1455
Cuts at: 5206 618
Size: 1842
Fragments arranged by size:
1842 1455 1102 627 467 325 233 217
162
BanII G_rGCy'C
Cuts at: 1391 2292 2400 2501 2686 2693 2721 2910 3152
Size: 901 108 101 185 7 28 189 242
Cuts at: 3152 3534 3655 3749 1391
Size: 382 121 94 4072
Fragments arranged by size:
4072 901 382 242 189 185 121 108
101 94 28 7
BbeI G_GCGC'C
Cuts at: 2258 3360 2258
Size: 1102 5328
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3071 928
Size: 971 1172 4287
Fragments arranged by size:
4287 1172 971
BbvCI CC'TCA_GC
Cuts at: 2050 2965 2050
Size: 915 5515
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4621 4655 1903
Size: 34 2684 34 3678
Fragments arranged by size:
3678 2684 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BfrBI ATG'CAT
Cuts at: 2956 2956
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2060 2111 2395
Size: 107 122 71 631 605 388 51 284
Cuts at: 2395 2627 2697 5041 136
Size: 232 70 2344 1525
Fragments arranged by size:
2344 1525 631 605 388 284 232 122
107 71 70 51
BglII A'GATC_T
Cuts at: 3576 6425 3576
Size: 2849 3581
BlpI GC'TnA_GC
Cuts at: 2904 3129 3533 3847 2904
Size: 225 404 314 5487
Fragments arranged by size:
5487 404 314 225
Bme1580I G_kGCm'C
Cuts at: 1790 2292 2400 2650 2686 2693 2795 2921 3152
Size: 502 108 250 36 7 102 126 231
Cuts at: 3152 4491 5647 1790
Size: 1339 1156 2573
Fragments arranged by size:
2573 1339 1156 502 250 231 126 108
102 36 7
BmgBI CAC'GTC
Cuts at: 1318 2985 1318
Size: 1667 4763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3463 3487 4161
Size: 416 53 83 399 24 2046 24 674
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
2046 1753 958 674 416 399 83 53
24 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2101 2578 2735 2752 2954 3097 3364 3650
Size: 862 477 157 17 202 143 267 286
Cuts at: 3650 5090 1239
Size: 1440 2579
Fragments arranged by size:
2579 1440 862 477 286 267 202 157
143 17
Bpu10I CC'TnA_GC
Cuts at: 2050 2965 2050
Size: 915 5515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 4555 5420 5718 2822
Size: 1733 865 298 3534
Fragments arranged by size:
3534 1733 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2510 3551 5093 882
Size: 617 1011 1041 1542 2219
Fragments arranged by size:
2219 1542 1041 1011 617
BsaAI yAC'GTr
Cuts at: 493 1204 3208 3388 4221 493
Size: 711 2004 180 833 2702
Fragments arranged by size:
2702 2004 833 711 180
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2255 2309 3357 4237
Size: 53 83 186 936 722 54 1048 880
Cuts at: 4237 4619 5254 275
Size: 382 635 1451
Fragments arranged by size:
1451 1048 936 880 722 635 382 186
83 54 53
BsaWI w'CCGG_w
Cuts at: 2847 4863 5261 5535 5682 6093 2847
Size: 2016 398 274 147 411 3184
Fragments arranged by size:
3184 2016 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 3668 3698 1178
Size: 30 165 30 1212 30 1023 30 3910
Fragments arranged by size:
3910 1212 1023 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2020 2384 1186
Size: 834 364 5232
Fragments arranged by size:
5232 834 364
BseYI C'CCAG_C
Cuts at: 2293 5633 6129 2293
Size: 3340 496 2594
Fragments arranged by size:
3340 2594 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3302 1163
Size: 573 1566 4291
Fragments arranged by size:
4291 1566 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2390 2457 2707 3306 3880 4641
Size: 275 186 389 67 250 599 574 761
Cuts at: 4641 5669 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 599 574 389 275 250
186 67
BsiHKAI G_wGCw'C
Cuts at: 2721 2795 2910 2921 2942 3213 4491 4576 5647
Size: 74 115 11 21 271 1278 85 1071
Cuts at: 5647 2721
Size: 3504
Fragments arranged by size:
3504 1278 1071 271 115 85 74 21
11
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
BsmBI CGTCTCn'nnnn_
Cuts at: 2868 3182 3403 2868
Size: 314 221 5895
Fragments arranged by size:
5895 314 221
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2292 2400 2501 2650 2686 2693 2721
Size: 399 502 108 101 149 36 7 28
Cuts at: 2721 2795 2910 2921 2942 3152 3213 3534 3655
Size: 74 115 11 21 210 61 321 121
Cuts at: 3655 3749 4491 4576 5647 1391
Size: 94 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 502 399 321 210 149
121 115 108 101 94 85 74 61
36 28 21 11 7
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 4319 6049 4319
Size: 1730 4700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3252 3705 3785 844
Size: 2408 453 80 3489
Fragments arranged by size:
3489 2408 453 80
BsrBI CCG'CTC
Cuts at: 1394 1823 2451 3265 4317 1394
Size: 429 628 814 1052 3507
Fragments arranged by size:
3507 1052 814 628 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4925 5099 65
Size: 1183 3677 174 1396
Fragments arranged by size:
3677 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5074 5261 1700
Size: 110 3264 187 2869
Fragments arranged by size:
3264 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 2179 1925
Size: 254 6176
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 5234 5234
Size: 6430
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6430
BstXI CCAn_nnnn'nTGG
Cuts at: 3388 3388
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 3576 3760 4127 4512 4529 5981 6067 6079
Size: 1604 184 367 385 17 1452 86 12
Cuts at: 6079 6425 1972
Size: 346 1977
Fragments arranged by size:
1977 1604 1452 385 367 346 184 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2072 2284 2384 2634 3100 513
Size: 552 1007 212 100 250 466 3843
Fragments arranged by size:
3843 1007 552 466 250 212 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5691
Fragments arranged by size:
5691 683 56
BtsI GCAGTG_nn'
Cuts at: 3946 4740 4760 3946
Size: 794 20 5616
Fragments arranged by size:
5616 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 3589 3589
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2387 2454 2538
Size: 54 1425 50 275 18 557 67 84
Cuts at: 2538 2619 3609 3877 4766 6395 8
Size: 81 990 268 889 1629 43
Fragments arranged by size:
1629 1425 990 889 557 275 268 84
81 67 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2387 2454 3877 1537
Size: 275 575 67 1423 4090
Fragments arranged by size:
4090 1423 575 275 67
EarI CTCTTCn'nnn_
Cuts at: 1353 2044 2219 2726 3437 4360 1353
Size: 691 175 507 711 923 3423
Fragments arranged by size:
3423 923 711 691 507 175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2420 2432 5017 5391 5537 387
Size: 1064 138 831 12 2585 374 146 1280
Fragments arranged by size:
2585 1280 1064 831 374 146 138 12
EcoICRI GAG'CTC
Cuts at: 2719 2908 2719
Size: 189 6241
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2101 2578 2612 2735 2752 2855 2954
Size: 393 469 477 34 123 17 103 99
Cuts at: 2954 3097 3364 3650 4493 5090 5877 6145 1239
Size: 143 267 286 843 597 787 268 1524
Fragments arranged by size:
1524 843 787 597 477 469 393 286
268 267 143 123 103 99 34 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2351 2351
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2289 2324 2397 2683 2690 3148 3149 2289
Size: 35 73 286 7 458 1 5570
Fragments arranged by size:
5570 458 286 73 35 7 1
EcoRI G'AATT_C
Cuts at: 2890 3739 4117 2890
Size: 849 378 5203
Fragments arranged by size:
5203 849 378
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2224 2258 2591 2675 3334 3360 5577 1678
Size: 546 34 333 84 659 26 2217 2531
Fragments arranged by size:
2531 2217 659 546 333 84 34 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2615 2647 3449
Size: 32 135 32 605 32 406 32 802
Cuts at: 3449 3481 3668 3700 5071 5103 5145 5177 1373
Size: 32 187 32 1371 32 42 32 2626
Fragments arranged by size:
2626 1371 802 605 406 187 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3774 4020 669
Size: 988 281 1836 246 3079
Fragments arranged by size:
3079 1836 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KasI G'GCGC_C
Cuts at: 2254 3356 2254
Size: 1102 5328
KpnI G_GTAC'C
Cuts at: 3755 3755
Size: 6430
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5544 5728 1305
Size: 4239 184 2007
Fragments arranged by size:
4239 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2540 3611 10
Size: 54 1425 343 708 1071 2829
Fragments arranged by size:
2829 1425 1071 708 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2828 3349 4388 4747 4906 518
Size: 648 1662 521 1039 359 159 2042
Fragments arranged by size:
2042 1662 1039 648 521 359 159
MspA1I CmG'CkG
Cuts at: 1606 2074 2176 2386 2471 2483 2643 2887 3133
Size: 468 102 210 85 12 160 244 246
Cuts at: 3133 3142 3846 4523 5671 5916 1606
Size: 9 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 468 246 245 244
210 160 102 85 12 9
NarI GG'CG_CC
Cuts at: 2255 3357 2255
Size: 1102 5328
NcoI C'CATG_G
Cuts at: 513 1065 2634 513
Size: 552 1569 4309
Fragments arranged by size:
4309 1569 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 2387 3877 2387
Size: 1490 4940
NsiI A_TGCA'T
Cuts at: 2958 2958
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3316 3788 5333 1132
Size: 97 2087 472 1545 2229
Fragments arranged by size:
2229 2087 1545 472 97
PasI CC'CwG_GG
Cuts at: 1348 3551 1348
Size: 2203 4227
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6430
PfoI T'CCnGG_A
Cuts at: 2775 3686 2775
Size: 911 5519
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2288 2396 2682 2689 3148 2288
Size: 108 286 7 459 5570
Fragments arranged by size:
5570 459 286 108 7
PspXI vC'TCGA_Gb
Cuts at: 3347 3347
Size: 6430
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2129 2161 2129
Size: 32 6398
PstI C_TGCA'G
Cuts at: 830 2025 2202 3147 3624 3782 830
Size: 1195 177 945 477 158 3478
Fragments arranged by size:
3478 1195 945 477 177 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2887 3133 3142 2887
Size: 246 9 6175
Fragments arranged by size:
6175 246 9
SacI G_AGCT'C
Cuts at: 2721 2910 2721
Size: 189 6241
SacII CC_GC'GG
Cuts at: 2075 2387 2075
Size: 312 6118
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SapI GCTCTTCn'nnn_
Cuts at: 2726 2726
Size: 6430
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 2138 4678 6390 2138
Size: 2540 1712 2178
Fragments arranged by size:
2540 2178 1712
SexAI A'CCwGG_T
Cuts at: 3409 3585 3409
Size: 176 6254
SfcI C'TryA_G
Cuts at: 826 1045 2021 2198 2611 3143 3620 3778 4913
Size: 219 976 177 413 532 477 158 1135
Cuts at: 4913 5594 5785 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 976 681 532 477 413 219
191 177 158
SfiI GGCCn_nnn'nGGCC
Cuts at: 2395 2627 2697 2395
Size: 232 70 6128
Fragments arranged by size:
6128 232 70
SfoI GGC'GCC
Cuts at: 2256 3358 2256
Size: 1102 5328
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6430
SmaI CCC'GGG
Cuts at: 1872 2120 2153 2395 2494 2570 2688 3527 3656
Size: 248 33 242 99 76 118 839 129
Cuts at: 3656 3757 1872
Size: 101 4545
Fragments arranged by size:
4545 839 248 242 129 118 101 99
76 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 3347 4534 5435 5697 820
Size: 197 926 858 546 1187 901 262 1553
Fragments arranged by size:
1553 1187 926 901 858 546 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SrfI GCCC'GGGC
Cuts at: 2395 2395
Size: 6430
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 2630 1517
Size: 1113 5317
StyI C'CwwG_G
Cuts at: 513 1065 2298 2352 2634 2996 3022 3869 513
Size: 552 1233 54 282 362 26 847 3074
Fragments arranged by size:
3074 1233 847 552 362 282 54 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2746 3292 4457 4474 4627 4812 5257
Size: 1255 1166 546 1165 17 153 185 445
Cuts at: 5257 325
Size: 1498
Fragments arranged by size:
1498 1255 1166 1165 546 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2136 3717 4676
Size: 274 80 33 51 1443 159 1581 959
Cuts at: 4676 6388 96
Size: 1712 138
Fragments arranged by size:
1712 1581 1443 959 274 159 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6390 6422 6390
Size: 32 6398
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XhoI C'TCGA_G
Cuts at: 1943 3347 1943
Size: 1404 5026
XmaI C'CCGG_G
Cuts at: 1870 2118 2151 2393 2492 2568 2686 3525 3654
Size: 248 33 242 99 76 118 839 129
Cuts at: 3654 3755 1870
Size: 101 4545
Fragments arranged by size:
4545 839 248 242 129 118 101 99
76 33
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4238 276
Size: 53 83 186 936 2704 2468
Fragments arranged by size:
2704 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PpuMI RsrII SanDI SwaI XcmI