Restriction map for
pFN21AE1994
(Circular) (Six-base) MAPSORT of: pFN21AE1994.seq Check: 4822 from: 1 to: 6427
pFN21AE1994 6427 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4237 278
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2160 3770 1937
Size: 223 1610 4594
Fragments arranged by size:
4594 1610 223
Acc65I G'GTAC_C
Cuts at: 3748 3748
Size: 6427
AclI AA'CG_TT
Cuts at: 1563 4554 4927 1563
Size: 2991 373 3063
Fragments arranged by size:
3063 2991 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3281 4490 5874 6142 1632
Size: 1649 1209 1384 268 1917
Fragments arranged by size:
1917 1649 1384 1209 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6230
AflIII A'CryG_T
Cuts at: 1203 2649 5326 6303 1203
Size: 1446 2677 977 1327
Fragments arranged by size:
2677 1446 1327 977
AgeI A'CCGG_T
Cuts at: 5258 5258
Size: 6427
AhdI GACnn_n'nnGTC
Cuts at: 5156 5156
Size: 6427
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2758 1184
Size: 1574 4853
AlwNI CAG_nnn'CTG
Cuts at: 2827 3406 3647 4170 5742 2827
Size: 579 241 523 1572 3512
Fragments arranged by size:
3512 1572 579 523 241
ApaI G_GGCC'C
Cuts at: 3087 3087
Size: 6427
ApaLI G'TGCA_C
Cuts at: 4484 5640 4484
Size: 1156 5271
ApoI r'AATT_y
Cuts at: 1493 2090 3736 3962 4114 6223 1493
Size: 597 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 597 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4981 160
Size: 872 3949 1606
Fragments arranged by size:
3949 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
AvaI C'yCGr_G
Cuts at: 1870 1943 3752 1870
Size: 73 1809 4545
Fragments arranged by size:
4545 1809 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3458 3491 3458
Size: 33 6394
BamHI G'GATC_C
Cuts at: 2036 3757 4124 2036
Size: 1721 367 4339
Fragments arranged by size:
4339 1721 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2600 3748 5203 618
Size: 325 217 627 357 456 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 456 357 325 217
BanII G_rGCy'C
Cuts at: 1391 2277 2511 2950 3087 3641 3746 1391
Size: 886 234 439 137 554 105 4072
Fragments arranged by size:
4072 886 554 439 234 137 105
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 2669 928
Size: 971 582 188 4686
Fragments arranged by size:
4686 971 582 188
BbvCI CC'TCA_GC
Cuts at: 2870 2870
Size: 6427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4618 4652 1903
Size: 34 2681 34 3678
Fragments arranged by size:
3678 2681 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4319 5529 4319
Size: 1210 5217
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6427
BfrBI ATG'CAT
Cuts at: 3222 3222
Size: 6427
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3143 5038 136
Size: 107 122 71 631 605 1471 1895 1525
Fragments arranged by size:
1895 1525 1471 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6422 6422
Size: 6427
BlpI GC'TnA_GC
Cuts at: 3844 3844
Size: 6427
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2644 3087 4488 5644 1790
Size: 357 497 443 1401 1156 2573
Fragments arranged by size:
2573 1401 1156 497 443 357
BmgBI CAC'GTC
Cuts at: 1318 2124 2848 1318
Size: 806 724 4897
Fragments arranged by size:
4897 806 724
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2206 3261 4158
Size: 416 53 83 399 24 789 1055 897
Cuts at: 4158 5116 442
Size: 958 1753
Fragments arranged by size:
1753 1055 958 897 789 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6427
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2083 3373 2083
Size: 1290 5137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2495 2993 3623 3683 5087 1239
Size: 824 432 498 630 60 1404 2579
Fragments arranged by size:
2579 1404 824 630 498 432 60
Bpu10I CC'TnA_GC
Cuts at: 2750 2870 2750
Size: 120 6307
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2351 3165 4552 5417 5715 2351
Size: 814 1387 865 298 3063
Fragments arranged by size:
3063 1387 865 814 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3644 5090 882
Size: 617 2145 1446 2219
Fragments arranged by size:
2219 2145 1446 617
BsaAI yAC'GTr
Cuts at: 493 1204 2086 4218 493
Size: 711 882 2132 2702
Fragments arranged by size:
2702 2132 882 711
BsaBI GATnn'nnATC
Cuts at: 6421 6421
Size: 6427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4234 4616 5251 275
Size: 53 83 186 936 2701 382 635 1451
Fragments arranged by size:
2701 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2676 2699 3190 4860 5258 5532 5679 6090 2676
Size: 23 491 1670 398 274 147 411 3013
Fragments arranged by size:
3013 1670 491 411 398 274 147 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 1178
Size: 30 165 30 1278 30 4894
Fragments arranged by size:
4894 1278 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3488 3493 1186
Size: 2302 5 4120
Fragments arranged by size:
4120 2302 5
BseYI C'CCAG_C
Cuts at: 2879 2892 3640 5630 6126 2879
Size: 13 748 1990 496 3180
Fragments arranged by size:
3180 1990 748 496 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2466 2763 3734 1163
Size: 573 730 297 971 3856
Fragments arranged by size:
3856 971 730 573 297
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3877 4638 5666 1540
Size: 275 186 1876 761 1028 2301
Fragments arranged by size:
2301 1876 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 2511 2847 2950 4488 4573 5644 2277
Size: 234 336 103 1538 85 1071 3060
Fragments arranged by size:
3060 1538 1071 336 234 103 85
BsiWI C'GTAC_G
Cuts at: 2181 2181
Size: 6427
BsmI GAATG_Cn'
Cuts at: 3938 4031 3938
Size: 93 6334
BsmBI CGTCTCn'nnnn_
Cuts at: 2249 2249
Size: 6427
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2277 2511 2644 2847 2950 3087
Size: 399 357 130 234 133 203 103 137
Cuts at: 3087 3641 3746 4488 4573 5644 1391
Size: 554 105 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 554 399 357 234 203
137 133 130 105 103 85
BspEI T'CCGG_A
Cuts at: 2699 3190 6090 2699
Size: 491 2900 3036
Fragments arranged by size:
3036 2900 491
BspHI T'CATG_A
Cuts at: 2607 4316 6046 2607
Size: 1709 1730 2988
Fragments arranged by size:
2988 1730 1709
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3782 844
Size: 2938 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4314 1394
Size: 429 2491 3507
Fragments arranged by size:
3507 2491 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3392 4922 5096 65
Size: 1183 2144 1530 174 1396
Fragments arranged by size:
2144 1530 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 2460 5071 5258 1700
Size: 110 503 147 2611 187 2869
Fragments arranged by size:
2869 2611 503 187 147 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6427
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6427
BssSI C'ACGA_G
Cuts at: 4487 5499 4487
Size: 1012 5415
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6427
BstBI TT'CG_AA
Cuts at: 5231 5231
Size: 6427
BstEII G'GTnAC_C
Cuts at: 1184 2290 3106 3352 1184
Size: 1106 816 246 4259
Fragments arranged by size:
4259 1106 816 246
BstXI CCAn_nnnn'nTGG
Cuts at: 2887 3591 3679 2887
Size: 704 88 5635
Fragments arranged by size:
5635 704 88
BstYI r'GATC_y
Cuts at: 1972 2036 2071 3036 3757 4124 4509 4526 5978
Size: 64 35 965 721 367 385 17 1452
Cuts at: 5978 6064 6076 6422 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 965 721 385 367 346 86
64 35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2058 3075 3102 3278 2058
Size: 1017 27 176 5207
Fragments arranged by size:
5207 1017 176 27
BtgI C'CryG_G
Cuts at: 513 1065 2718 513
Size: 552 1653 4222
Fragments arranged by size:
4222 1653 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2466 487
Size: 683 56 1240 4448
Fragments arranged by size:
4448 1240 683 56
BtsI GCAGTG_nn'
Cuts at: 2666 3722 3943 4737 4757 2666
Size: 1056 221 794 20 4336
Fragments arranged by size:
4336 1056 794 221 20
ClaI AT'CG_AT
Cuts at: 1554 2405 1554
Size: 851 5576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6392
DraI TTT'AAA
Cuts at: 3731 3731
Size: 6427
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5434 809
Size: 507 4118 1802
Fragments arranged by size:
4118 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2455 3874 4763
Size: 54 1425 50 275 18 625 1419 889
Cuts at: 4763 6392 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1419 889 625 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3874 1537
Size: 275 2062 4090
Fragments arranged by size:
4090 2062 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4357 1353
Size: 3004 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5014 5388 5534 387
Size: 1064 138 3425 374 146 1280
Fragments arranged by size:
3425 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2275 2509 2948 2275
Size: 234 439 5754
Fragments arranged by size:
5754 439 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2495 2993 3281 3623 3683 4490
Size: 393 431 432 498 288 342 60 807
Cuts at: 4490 5087 5874 6142 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 498 432 431 393
342 288 268 60
EcoO109I rG'GnC_Cy
Cuts at: 2062 3084 3595 2062
Size: 1022 511 4894
Fragments arranged by size:
4894 1022 511
EcoRI G'AATT_C
Cuts at: 3736 4114 3736
Size: 378 6049
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3311 3311
Size: 6427
FspI TGC'GCA
Cuts at: 4933 4933
Size: 6427
HaeII r_GCGC'y
Cuts at: 1678 5574 1678
Size: 3896 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2407 2439 3413 3445 5068
Size: 32 135 32 835 32 974 32 1623
Cuts at: 5068 5100 5142 5174 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1623 974 835 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2518 3771 4017 669
Size: 988 281 580 1253 246 3079
Fragments arranged by size:
3079 1253 988 580 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6427
HpaI GTT'AAC
Cuts at: 4017 4017
Size: 6427
KpnI G_GTAC'C
Cuts at: 3752 3752
Size: 6427
MfeI C'AATT_G
Cuts at: 4026 4026
Size: 6427
MluI A'CGCG_T
Cuts at: 6303 6303
Size: 6427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3042 5541 5725 1305
Size: 1737 2499 184 2007
Fragments arranged by size:
2499 2007 1737 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2457 10
Size: 54 1425 343 625 3980
Fragments arranged by size:
3980 1425 625 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2327 2606 4385 4744 4903 518
Size: 648 1161 279 1779 359 159 2042
Fragments arranged by size:
2042 1779 1161 648 359 279 159
MspA1I CmG'CkG
Cuts at: 1606 2133 2221 2297 2827 2874 2956 3198 3314
Size: 527 88 76 530 47 82 242 116
Cuts at: 3314 3843 4520 5668 5913 1606
Size: 529 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 530 529 527 245 242
116 88 82 76 47
NcoI C'CATG_G
Cuts at: 513 1065 2718 513
Size: 552 1653 4222
Fragments arranged by size:
4222 1653 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6427
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6427
NotI GC'GGCC_GC
Cuts at: 3874 3874
Size: 6427
NsiI A_TGCA'T
Cuts at: 3224 3224
Size: 6427
NspI r_CATG'y
Cuts at: 1132 1229 2653 3328 3785 5330 1132
Size: 97 1424 675 457 1545 2229
Fragments arranged by size:
2229 1545 1424 675 457 97
PasI CC'CwG_GG
Cuts at: 1348 2931 3406 3591 1348
Size: 1583 475 185 4184
Fragments arranged by size:
4184 1583 475 185
PciI A'CATG_T
Cuts at: 2649 5326 2649
Size: 2677 3750
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3314 3406 1108
Size: 2206 92 4129
Fragments arranged by size:
4129 2206 92
PfoI T'CCnGG_A
Cuts at: 2838 2838
Size: 6427
PmeI GTTT'AAAC
Cuts at: 3731 3731
Size: 6427
PmlI CAC'GTG
Cuts at: 2086 2086
Size: 6427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4496 4528 6009 6041 4496
Size: 32 1481 32 4882
Fragments arranged by size:
4882 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2062 2062
Size: 6427
PshAI GACnn'nnGTC
Cuts at: 1613 3579 1613
Size: 1966 4461
PsiI TTA'TAA
Cuts at: 3997 3997
Size: 6427
PspOMI G'GGCC_C
Cuts at: 3083 3083
Size: 6427
PstI C_TGCA'G
Cuts at: 830 2355 2944 3348 3779 830
Size: 1525 589 404 431 3478
Fragments arranged by size:
3478 1525 589 431 404
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6427
PvuII CAG'CTG
Cuts at: 3198 3314 3198
Size: 116 6311
SacI G_AGCT'C
Cuts at: 2277 2511 2950 2277
Size: 234 439 5754
Fragments arranged by size:
5754 439 234
SalI G'TCGA_C
Cuts at: 1936 3769 1936
Size: 1833 4594
SbfI CC_TGCA'GG
Cuts at: 2944 3779 2944
Size: 835 5592
ScaI AGT'ACT
Cuts at: 2739 2906 4675 6387 2739
Size: 167 1769 1712 2779
Fragments arranged by size:
2779 1769 1712 167
SfcI C'TryA_G
Cuts at: 826 1045 2161 2351 2755 2940 3344 3775 4910
Size: 219 1116 190 404 185 404 431 1135
Cuts at: 4910 5591 5782 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1116 681 431 404 404 219
191 190 185
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6427
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6427
SmaI CCC'GGG
Cuts at: 1872 3754 1872
Size: 1882 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2366 3180 4531 5432 5694 820
Size: 197 926 423 814 1351 901 262 1553
Fragments arranged by size:
1553 1351 926 901 814 423 262 197
SnaBI TAC'GTA
Cuts at: 493 4218 493
Size: 3725 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6427
SphI G_CATG'C
Cuts at: 1132 3785 1132
Size: 2653 3774
SspI AAT'ATT
Cuts at: 5 52 4351 5
Size: 47 4299 2081
Fragments arranged by size:
4299 2081 47
StuI AGG'CCT
Cuts at: 1517 3283 3699 1517
Size: 1766 416 4245
Fragments arranged by size:
4245 1766 416
StyI C'CwwG_G
Cuts at: 513 1065 2539 2596 2631 2718 3360 3577 3621
Size: 552 1474 57 35 87 642 217 44
Cuts at: 3621 3866 513
Size: 245 3074
Fragments arranged by size:
3074 1474 642 552 245 217 87 57
44 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2188 4454 4471 4624 4809 5254 325
Size: 1255 608 2266 17 153 185 445 1498
Fragments arranged by size:
2266 1498 1255 608 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2737 2904 4673
Size: 274 80 33 51 1443 760 167 1769
Cuts at: 4673 6385 96
Size: 1712 138
Fragments arranged by size:
1769 1712 1443 760 274 167 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3515 3547 6387 6419 3515
Size: 32 2840 32 3523
Fragments arranged by size:
3523 2840 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2156 1316
Size: 840 5587
XbaI T'CTAG_A
Cuts at: 3763 3763
Size: 6427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2537 2679 2029
Size: 508 142 5777
Fragments arranged by size:
5777 508 142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6427
XmaI C'CCGG_G
Cuts at: 1870 3752 1870
Size: 1882 4545
XmnI GAAnn'nnTTC
Cuts at: 4556 4556
Size: 6427
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4235 276
Size: 53 83 186 936 2701 2468
Fragments arranged by size:
2701 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BstZ17I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI