Restriction map for
pFN21AE1993
(Circular) (Six-base) MAPSORT of: pFN21AE1993.seq Check: 826 from: 1 to: 6421
pFN21AE1993 6421 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2220 2258 3569 4231
Size: 53 83 186 936 684 38 1311 662
Cuts at: 4231 278
Size: 2468
Fragments arranged by size:
2468 1311 936 684 662 186 83 53
38
AccI GT'mk_AC
Cuts at: 1937 3764 1937
Size: 1827 4594
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4484 5868 6136 1632
Size: 2852 1384 268 1917
Fragments arranged by size:
2852 1917 1384 268
AfeI AGC'GCT
Cuts at: 2466 2466
Size: 6421
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 2671 5320 6297 1203
Size: 1468 2649 977 1327
Fragments arranged by size:
2649 1468 1327 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 2392 5150 2392
Size: 2758 3663
AleI CACnn'nnGTG
Cuts at: 1166 2765 1166
Size: 1599 4822
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 4164 5736 4164
Size: 1572 4849
ApaI G_GGCC'C
Cuts at: 2663 2663
Size: 6421
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 2488 3730 3956 4108 6217 1493
Size: 995 1242 226 152 2109 1697
Fragments arranged by size:
2109 1697 1242 995 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 2663 3290 3746 1870
Size: 73 720 627 456 4545
Fragments arranged by size:
4545 720 627 456 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3430 3463 3430
Size: 33 6388
BamHI G'GATC_C
Cuts at: 2637 3215 3751 4118 2637
Size: 578 536 367 4940
Fragments arranged by size:
4940 578 536 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2579 3065 3374 3682 3742
Size: 325 217 627 792 486 309 308 60
Cuts at: 3742 5197 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 792 627 486 325 309 308
217 60
BanII G_rGCy'C
Cuts at: 1391 2663 2838 2930 3740 1391
Size: 1272 175 92 810 4072
Fragments arranged by size:
4072 1272 810 175 92
BbeI G_GCGC'C
Cuts at: 2583 3686 2583
Size: 1103 5318
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2045 2177 3169 928
Size: 971 146 132 992 4180
Fragments arranged by size:
4180 992 971 146 132
BbvCI CC'TCA_GC
Cuts at: 2787 2787
Size: 6421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2600 2634 4612 4646 1903
Size: 34 663 34 1978 34 3678
Fragments arranged by size:
3678 1978 663 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 2058 2127 2242 2313 1548
Size: 510 69 115 71 5656
Fragments arranged by size:
5656 510 115 71 69
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3026 3557 5032
Size: 107 122 71 631 605 1354 531 1475
Cuts at: 5032 136
Size: 1525
Fragments arranged by size:
1525 1475 1354 631 605 531 122 107
71
BglII A'GATC_T
Cuts at: 6416 6416
Size: 6421
BlpI GC'TnA_GC
Cuts at: 2678 3471 3838 2678
Size: 793 367 5261
Fragments arranged by size:
5261 793 367
Bme1580I G_kGCm'C
Cuts at: 1790 2663 3001 3068 4482 5638 1790
Size: 873 338 67 1414 1156 2573
Fragments arranged by size:
2573 1414 1156 873 338 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2896 3129 4152
Size: 416 53 83 399 24 1479 233 1023
Cuts at: 4152 5110 442
Size: 958 1753
Fragments arranged by size:
1753 1479 1023 958 416 399 233 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2203 2203
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2896 2928 2966 3386 3531 5081 1239
Size: 1657 32 38 420 145 1550 2579
Fragments arranged by size:
2579 1657 1550 420 145 38 32
Bpu10I CC'TnA_GC
Cuts at: 2334 2787 2334
Size: 453 5968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2944 4546 5411 5709 2944
Size: 1602 865 298 3656
Fragments arranged by size:
3656 1602 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2970 5084 882
Size: 617 1471 2114 2219
Fragments arranged by size:
2219 2114 1471 617
BsaAI yAC'GTr
Cuts at: 493 1204 4212 493
Size: 711 3008 2702
Fragments arranged by size:
3008 2702 711
BsaBI GATnn'nnATC
Cuts at: 2198 6415 2198
Size: 4217 2204
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2217 2255 2580 3530
Size: 53 83 186 936 684 38 325 950
Cuts at: 3530 3566 3683 4228 4610 5245 275
Size: 36 117 545 382 635 1451
Fragments arranged by size:
1451 950 936 684 635 545 382 325
186 117 83 53 38 36
BsaWI w'CCGG_w
Cuts at: 3058 3597 4854 5252 5526 5673 6084 3058
Size: 539 1257 398 274 147 411 3395
Fragments arranged by size:
3395 1257 539 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3074 3104 3529 3559 1178
Size: 30 165 30 1671 30 425 30 4040
Fragments arranged by size:
4040 1671 425 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2178 2468 2639 2705 3095 3544 1186
Size: 992 290 171 66 390 449 4063
Fragments arranged by size:
4063 992 449 390 290 171 66
BseYI C'CCAG_C
Cuts at: 2297 3669 5624 6120 2297
Size: 1372 1955 496 2598
Fragments arranged by size:
2598 1955 1372 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3590 1163
Size: 573 1854 3994
Fragments arranged by size:
3994 1854 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2050 3871 4632 5660 1540
Size: 275 186 49 1821 761 1028 2301
Fragments arranged by size:
2301 1821 1028 761 275 186 49
BsiHKAI G_wGCw'C
Cuts at: 3637 4482 4567 5638 3637
Size: 845 85 1071 4420
Fragments arranged by size:
4420 1071 845 85
BsmI GAATG_Cn'
Cuts at: 3932 4025 3932
Size: 93 6328
BsmBI CGTCTCn'nnnn_
Cuts at: 3573 3573
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2663 2838 2930 3001 3068 3637 3740
Size: 399 873 175 92 71 67 569 103
Cuts at: 3740 4482 4567 5638 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 873 742 569 399 175 103
92 85 71 67
BspEI T'CCGG_A
Cuts at: 3058 3597 6084 3058
Size: 539 2487 3395
Fragments arranged by size:
3395 2487 539
BspHI T'CATG_A
Cuts at: 4310 6040 4310
Size: 1730 4691
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3612 3776 844
Size: 2768 164 3489
Fragments arranged by size:
3489 2768 164
BsrBI CCG'CTC
Cuts at: 1394 1823 3085 4308 1394
Size: 429 1262 1223 3507
Fragments arranged by size:
3507 1262 1223 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2616 3001 3204 4916 5090 65
Size: 1183 1368 385 203 1712 174 1396
Fragments arranged by size:
1712 1396 1368 1183 385 203 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5065 5252 1700
Size: 110 3255 187 2869
Fragments arranged by size:
3255 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2502 96
Size: 2406 4015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 2041 2498 3489 4481 5493 2041
Size: 457 991 992 1012 2969
Fragments arranged by size:
2969 1012 992 991 457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2397 3318 794
Size: 1603 921 3897
Fragments arranged by size:
3897 1603 921
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 2637 2644 3215 3751 4118 4503 4520 5972
Size: 665 7 571 536 367 385 17 1452
Cuts at: 5972 6058 6070 6416 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 665 571 536 385 367 346
86 17 12 7
Bsu36I CC'TnA_GG
Cuts at: 2688 2688
Size: 6421
BtgI C'CryG_G
Cuts at: 513 1065 3322 513
Size: 552 2257 3612
Fragments arranged by size:
3612 2257 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2713 2779 487
Size: 683 56 1487 66 4129
Fragments arranged by size:
4129 1487 683 66 56
BtsI GCAGTG_nn'
Cuts at: 2841 3001 3015 3937 4731 4751 2841
Size: 160 14 922 794 20 4511
Fragments arranged by size:
4511 922 794 160 20 14
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3725 3725
Size: 6421
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2984 5428 809
Size: 507 1668 2444 1802
Fragments arranged by size:
2444 1802 1668 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3558 3694 3868
Size: 54 1425 50 275 18 1728 136 174
Cuts at: 3868 4757 6386 8
Size: 889 1629 43
Fragments arranged by size:
1728 1629 1425 889 275 174 136 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4351 1353
Size: 2998 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5008 5382 5528 387
Size: 1064 138 3419 374 146 1280
Fragments arranged by size:
3419 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2896 2928 2966 3386 3531 4484 5081
Size: 393 1264 32 38 420 145 953 597
Cuts at: 5081 5868 6136 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1264 953 787 597 420 393 268
145 38 32
EcoNI CCTnn'n_nnAGG
Cuts at: 3195 3195
Size: 6421
EcoO109I rG'GnC_Cy
Cuts at: 2660 3241 3586 2660
Size: 581 345 5495
Fragments arranged by size:
5495 581 345
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3061 3061
Size: 6421
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 2468 2583 3686 5568 1678
Size: 790 115 1103 1882 2531
Fragments arranged by size:
2531 1882 1103 790 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2035 2067 2075 2107 2227
Size: 32 135 32 463 32 8 32 120
Cuts at: 2227 2259 2378 2410 2629 2661 2917 2949 3074
Size: 32 119 32 219 32 256 32 125
Cuts at: 3074 3106 3529 3561 5062 5094 5136 5168 1373
Size: 32 423 32 1501 32 42 32 2626
Fragments arranged by size:
2626 1501 463 423 256 219 135 125
120 119 42 32 32 32 32 32
32 32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3765 4011 669
Size: 988 281 1827 246 3079
Fragments arranged by size:
3079 1827 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 4011 4011
Size: 6421
KasI G'GCGC_C
Cuts at: 2579 3682 2579
Size: 1103 5318
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 4020 4020
Size: 6421
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2960 5535 5719 1305
Size: 1655 2575 184 2007
Fragments arranged by size:
2575 2007 1655 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4599
Fragments arranged by size:
4599 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2765 3209 4379 4738 4897 518
Size: 648 1599 444 1170 359 159 2042
Fragments arranged by size:
2042 1599 1170 648 444 359 159
MspA1I CmG'CkG
Cuts at: 1606 2752 3324 3837 4514 5662 5907 1606
Size: 1146 572 513 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 677 572 513 245
NarI GG'CG_CC
Cuts at: 2580 3683 2580
Size: 1103 5318
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5869
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6421
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 2420 2675 3386 3779 5324 1132
Size: 97 1191 255 711 393 1545 2229
Fragments arranged by size:
2229 1545 1191 711 393 255 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6421
PciI A'CATG_T
Cuts at: 2671 5320 2671
Size: 2649 3772
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3145 1108
Size: 2037 4384
PfoI T'CCnGG_A
Cuts at: 2189 2448 2568 2640 3363 2189
Size: 259 120 72 723 5247
Fragments arranged by size:
5247 723 259 120 72
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3241 3241
Size: 6421
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6421
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PspOMI G'GGCC_C
Cuts at: 2659 2659
Size: 6421
PstI C_TGCA'G
Cuts at: 830 3773 830
Size: 2943 3478
PvuI CG_AT'CG
Cuts at: 2001 2050 2001
Size: 49 6372
SacII CC_GC'GG
Cuts at: 3325 3325
Size: 6421
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SanDI GG'GwC_CC
Cuts at: 3241 3241
Size: 6421
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 2556 4669 6381 2556
Size: 2113 1712 2596
Fragments arranged by size:
2596 2113 1712
SexAI A'CCwGG_T
Cuts at: 3228 3228
Size: 6421
SfcI C'TryA_G
Cuts at: 826 1045 3030 3769 4904 5585 5776 826
Size: 219 1985 739 1135 681 191 1471
Fragments arranged by size:
1985 1471 1135 739 681 219 191
SfoI GGC'GCC
Cuts at: 2581 3684 2581
Size: 1103 5318
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3748 1872
Size: 1876 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2923 3290 4525 5426 5688 820
Size: 197 926 980 367 1235 901 262 1553
Fragments arranged by size:
1553 1235 980 926 901 367 262 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 2029 4345 5
Size: 47 1977 2316 2081
Fragments arranged by size:
2316 2081 1977 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6421
StyI C'CwwG_G
Cuts at: 513 1065 2275 2283 2763 2892 3860 513
Size: 552 1210 8 480 129 968 3074
Fragments arranged by size:
3074 1210 968 552 480 129 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3683 4448 4465 4618 4803 5248 325
Size: 1255 2103 765 17 153 185 445 1498
Fragments arranged by size:
2103 1498 1255 765 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2554 3294
Size: 274 80 33 51 1443 525 52 740
Cuts at: 3294 4667 6379 96
Size: 1373 1712 138
Fragments arranged by size:
1712 1443 1373 740 525 274 138 80
52 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 3040 3072 6381 6413 2808
Size: 32 200 32 3309 32 2816
Fragments arranged by size:
3309 2816 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3230 1316
Size: 1914 4507
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2290 2603 2290
Size: 313 6108
XhoI C'TCGA_G
Cuts at: 1943 3290 1943
Size: 1347 5074
XmaI C'CCGG_G
Cuts at: 1870 3746 1870
Size: 1876 4545
XmnI GAAnn'nnTTC
Cuts at: 3422 4550 3422
Size: 1128 5293
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2218 2256 3567 4229
Size: 53 83 186 936 684 38 1311 662
Cuts at: 4229 276
Size: 2468
Fragments arranged by size:
2468 1311 936 684 662 186 83 53
38
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BfrBI BsiWI BstXI BstZ17I
DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI
SapI SfiI SrfI SwaI