Restriction map for
pFN21AE1989
(Circular) (Six-base) MAPSORT of: pFN21AE1989.seq Check: 6160 from: 1 to: 6400
pFN21AE1989 6400 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 3564 3743 1937
Size: 871 756 179 4594
Fragments arranged by size:
4594 871 756 179
Acc65I G'GTAC_C
Cuts at: 3721 3721
Size: 6400
AclI AA'CG_TT
Cuts at: 1563 4527 4900 1563
Size: 2964 373 3063
Fragments arranged by size:
3063 2964 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2998 4463 5847 6115 1632
Size: 1366 1465 1384 268 1917
Fragments arranged by size:
1917 1465 1384 1366 268
AfeI AGC'GCT
Cuts at: 2819 2930 3479 2819
Size: 111 549 5740
Fragments arranged by size:
5740 549 111
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6203
AflIII A'CryG_T
Cuts at: 1203 2726 5299 6276 1203
Size: 1523 2573 977 1327
Fragments arranged by size:
2573 1523 1327 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 5129 5129
Size: 6400
AleI CACnn'nnGTG
Cuts at: 1166 2299 2870 2957 1166
Size: 1133 571 87 4609
Fragments arranged by size:
4609 1133 571 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2039 2317 2544 2975 3653 1184
Size: 855 278 227 431 678 3931
Fragments arranged by size:
3931 855 678 431 278 227
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2307 2339 2307
Size: 32 6368
AlwNI CAG_nnn'CTG
Cuts at: 4143 5715 4143
Size: 1572 4828
ApaLI G'TGCA_C
Cuts at: 2143 2222 2302 4457 5613 2143
Size: 79 80 2155 1156 2930
Fragments arranged by size:
2930 2155 1156 80 79
ApoI r'AATT_y
Cuts at: 1493 3709 3935 4087 6196 1493
Size: 2216 226 152 2109 1697
Fragments arranged by size:
2216 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4954 160
Size: 872 3922 1606
Fragments arranged by size:
3922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 2402 2542 2551 3725 1870
Size: 73 459 140 9 1174 4545
Fragments arranged by size:
4545 1174 459 140 73 9
BamHI G'GATC_C
Cuts at: 2745 3730 4097 2745
Size: 985 367 5048
Fragments arranged by size:
5048 985 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2438 2528 2537 2623 2966
Size: 325 217 627 651 90 9 86 343
Cuts at: 2966 3350 3721 5176 618
Size: 384 371 1455 1842
Fragments arranged by size:
1842 1455 651 627 384 371 343 325
217 90 86 9
BanII G_rGCy'C
Cuts at: 1391 2485 3719 1391
Size: 1094 1234 4072
Fragments arranged by size:
4072 1234 1094
BbeI G_GCGC'C
Cuts at: 2442 2442
Size: 6400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3388 928
Size: 971 1489 3940
Fragments arranged by size:
3940 1489 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2134 2168 4591 4625 1903
Size: 34 50 34 113 34 2423 34 3678
Fragments arranged by size:
3678 2423 113 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4292 5502 4292
Size: 1210 5190
BclI T'GATC_A
Cuts at: 1548 3570 1548
Size: 2022 4378
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2069 2556 3101
Size: 107 122 71 631 605 397 487 545
Cuts at: 3101 3134 3143 5011 136
Size: 33 9 1868 1525
Fragments arranged by size:
1868 1525 631 605 545 487 397 122
107 71 33 9
BglII A'GATC_T
Cuts at: 2448 6395 2448
Size: 3947 2453
BlpI GC'TnA_GC
Cuts at: 3197 3817 3197
Size: 620 5780
Bme1580I G_kGCm'C
Cuts at: 1790 2147 2226 2306 2540 2969 3420 3524 4461
Size: 357 79 80 234 429 451 104 937
Cuts at: 4461 5617 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 937 451 429 357 234 104
80 79
BmgBI CAC'GTC
Cuts at: 1318 3288 3606 1318
Size: 1970 318 4112
Fragments arranged by size:
4112 1970 318
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2068 3481 4131
Size: 416 53 83 399 24 651 1413 650
Cuts at: 4131 5089 442
Size: 958 1753
Fragments arranged by size:
1753 1413 958 651 650 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3257 1056
Size: 2201 4199
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2950 3124 2950
Size: 174 6226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 2888 3389 5060 1239
Size: 1370 279 501 1671 2579
Fragments arranged by size:
2579 1671 1370 501 279
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2603 4525 5390 5688 2603
Size: 1922 865 298 3315
Fragments arranged by size:
3315 1922 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3683 5063 882
Size: 617 2184 1380 2219
Fragments arranged by size:
2219 2184 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 2799 4191 493
Size: 711 1595 1392 2702
Fragments arranged by size:
2702 1595 1392 711
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2439 3393 4207 4589
Size: 53 83 186 936 906 954 814 382
Cuts at: 4589 5224 275
Size: 635 1451
Fragments arranged by size:
1451 954 936 906 814 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4833 5231 5505 5652 6063 4833
Size: 398 274 147 411 5170
Fragments arranged by size:
5170 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3467 3497 1178
Size: 30 165 30 2064 30 4081
Fragments arranged by size:
4081 2064 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2735 1186
Size: 1549 4851
BseYI C'CCAG_C
Cuts at: 5603 6099 5603
Size: 496 5904
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2270 2968 2981 3623 1163
Size: 573 534 698 13 642 3940
Fragments arranged by size:
3940 698 642 573 534 13
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3147 3850 4611 5639 1540
Size: 275 186 1146 703 761 1028 2301
Fragments arranged by size:
2301 1146 1028 761 703 275 186
BsiHKAI G_wGCw'C
Cuts at: 2147 2226 2258 2285 2306 2424 2485 2595 2904
Size: 79 32 27 21 118 61 110 309
Cuts at: 2904 3408 3471 4461 4546 5617 2147
Size: 504 63 990 85 1071 2930
Fragments arranged by size:
2930 1071 990 504 309 118 110 85
79 63 61 32 27 21
BsmI GAATG_Cn'
Cuts at: 3911 4004 3911
Size: 93 6307
BsmBI CGTCTCn'nnnn_
Cuts at: 2717 2717
Size: 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2147 2226 2258 2285 2306 2424 2485
Size: 399 357 79 32 27 21 118 61
Cuts at: 2485 2540 2595 2904 2969 3408 3420 3471 3524
Size: 55 55 309 65 439 12 51 53
Cuts at: 3524 3719 4461 4546 5617 1391
Size: 195 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 439 399 357 309 195
118 85 79 65 61 55 55 53
51 32 27 21 12
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 2376 2997 3336 3582 4289 6019 2376
Size: 621 339 246 707 1730 2757
Fragments arranged by size:
2757 1730 707 621 339 246
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2692 3201 3755 844
Size: 1848 509 554 3489
Fragments arranged by size:
3489 1848 554 509
BsrBI CCG'CTC
Cuts at: 1394 1823 2337 2836 3201 3262 3373 4287 1394
Size: 429 514 499 365 61 111 914 3507
Fragments arranged by size:
3507 914 514 499 429 365 111 61
BsrDI GCAATG_nn'
Cuts at: 65 1248 4895 5069 65
Size: 1183 3647 174 1396
Fragments arranged by size:
3647 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 3071 3142 3302 5044 5231 1700
Size: 110 258 1003 71 160 1742 187 2869
Fragments arranged by size:
2869 1742 1003 258 187 160 110 71
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 2350 2352 2354 2735 1925
Size: 425 2 2 381 5590
Fragments arranged by size:
5590 425 381 2 2
BssSI C'ACGA_G
Cuts at: 3089 3481 3643 4460 5472 3089
Size: 392 162 817 1012 4017
Fragments arranged by size:
4017 1012 817 392 162
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6400
BstBI TT'CG_AA
Cuts at: 5204 5204
Size: 6400
BstEII G'GTnAC_C
Cuts at: 1184 2945 1184
Size: 1761 4639
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2803 2185
Size: 618 5782
BstYI r'GATC_y
Cuts at: 1972 2448 2745 3618 3730 4097 4482 4499 5951
Size: 476 297 873 112 367 385 17 1452
Cuts at: 5951 6037 6049 6395 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 873 476 385 367 346 297
112 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2080 2110 2434 2868 3171 3696 513
Size: 552 1015 30 324 434 303 525 3217
Fragments arranged by size:
3217 1015 552 525 434 324 303 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2137 2872 3418 487
Size: 683 56 911 735 546 3469
Fragments arranged by size:
3469 911 735 683 546 56
BtsI GCAGTG_nn'
Cuts at: 2258 2602 3496 3916 4710 4730 2258
Size: 344 894 420 794 20 3928
Fragments arranged by size:
3928 894 794 420 344 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6365
DraI TTT'AAA
Cuts at: 3704 3704
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3560 5407 809
Size: 507 2244 1847 1802
Fragments arranged by size:
2244 1847 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2458 3021 3126
Size: 54 1425 50 275 18 628 563 105
Cuts at: 3126 3144 3168 3300 3510 3847 4736 6365 8
Size: 18 24 132 210 337 889 1629 43
Fragments arranged by size:
1629 1425 889 628 563 337 275 210
132 105 54 50 43 24 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3144 3847 1537
Size: 275 1332 703 4090
Fragments arranged by size:
4090 1332 703 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2999 3269 3323 4330 1353
Size: 1646 270 54 1007 3423
Fragments arranged by size:
3423 1646 1007 270 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4987 5361 5507 387
Size: 1064 138 3398 374 146 1280
Fragments arranged by size:
3398 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2483 2483
Size: 6400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 2888 2998 3389 4463 5060 5847
Size: 393 977 279 110 391 1074 597 787
Cuts at: 5847 6115 1239
Size: 268 1524
Fragments arranged by size:
1524 1074 977 787 597 393 391 279
268 110
EcoRI G'AATT_C
Cuts at: 3709 4087 3709
Size: 378 6022
FseI GG_CCGG'CC
Cuts at: 2072 2072
Size: 6400
FspI TGC'GCA
Cuts at: 3386 4906 3386
Size: 1520 4880
FspAI rTGC'GCAy
Cuts at: 3386 3386
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 2022 2410 2442 2568 2821 2932 3260 3481
Size: 344 388 32 126 253 111 328 221
Cuts at: 3481 5547 1678
Size: 2066 2531
Fragments arranged by size:
2531 2066 388 344 328 253 221 126
111 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5041 5073 5115 5147 1373
Size: 32 135 32 3469 32 42 32 2626
Fragments arranged by size:
3469 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3744 3990 669
Size: 988 281 1806 246 3079
Fragments arranged by size:
3079 1806 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6400
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KasI G'GCGC_C
Cuts at: 2438 2438
Size: 6400
KpnI G_GTAC'C
Cuts at: 3725 3725
Size: 6400
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 2726 6276 2726
Size: 3550 2850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5514 5698 1305
Size: 4209 184 2007
Fragments arranged by size:
4209 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3023 3170 3512 10
Size: 54 1425 343 1191 147 342 2898
Fragments arranged by size:
2898 1425 1191 343 342 147 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2299 2870 2957 3335 4358 4717 4876
Size: 648 1133 571 87 378 1023 359 159
Cuts at: 4876 518
Size: 2042
Fragments arranged by size:
2042 1133 1023 648 571 378 359 159
87
MspA1I CmG'CkG
Cuts at: 1606 2044 2140 2239 3238 3816 4493 5641 5886
Size: 438 96 99 999 578 677 1148 245
Cuts at: 5886 1606
Size: 2120
Fragments arranged by size:
2120 1148 999 677 578 438 245 99
96
NaeI GCC'GGC
Cuts at: 2070 3073 3144 3304 2070
Size: 1003 71 160 5166
Fragments arranged by size:
5166 1003 160 71
NarI GG'CG_CC
Cuts at: 2439 2439
Size: 6400
NcoI C'CATG_G
Cuts at: 513 1065 2868 3171 513
Size: 552 1803 303 3742
Fragments arranged by size:
3742 1803 552 303
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6400
NgoMIV G'CCGG_C
Cuts at: 2068 3071 3142 3302 2068
Size: 1003 71 160 5166
Fragments arranged by size:
5166 1003 160 71
NheI G'CTAG_C
Cuts at: 1052 3253 1052
Size: 2201 4199
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 2815 3547 3758 5303 1132
Size: 97 1586 732 211 1545 2229
Fragments arranged by size:
2229 1586 1545 732 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6400
PciI A'CATG_T
Cuts at: 5299 5299
Size: 6400
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3036 1108
Size: 1928 4472
PfoI T'CCnGG_A
Cuts at: 2103 2904 2103
Size: 801 5599
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 3970 3970
Size: 6400
PstI C_TGCA'G
Cuts at: 830 2523 3752 830
Size: 1693 1229 3478
Fragments arranged by size:
3478 1693 1229
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
SacI G_AGCT'C
Cuts at: 2485 2485
Size: 6400
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SapI GCTCTTCn'nnn_
Cuts at: 3269 3323 3269
Size: 54 6346
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 3116 4648 6360 3116
Size: 1532 1712 3156
Fragments arranged by size:
3156 1712 1532
SexAI A'CCwGG_T
Cuts at: 2753 3056 2753
Size: 303 6097
SfcI C'TryA_G
Cuts at: 826 1045 2519 3748 4883 5564 5755 826
Size: 219 1474 1229 1135 681 191 1471
Fragments arranged by size:
1474 1471 1229 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3101 3134 3143 3101
Size: 33 9 6358
Fragments arranged by size:
6358 33 9
SfoI GGC'GCC
Cuts at: 2440 2440
Size: 6400
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 2544 2553 3727 1872
Size: 672 9 1174 4545
Fragments arranged by size:
4545 1174 672 9
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2618 4504 5405 5667 820
Size: 197 926 675 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 675 262 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SrfI GCCC'GGGC
Cuts at: 2553 2553
Size: 6400
SspI AAT'ATT
Cuts at: 5 52 4324 5
Size: 47 4272 2081
Fragments arranged by size:
4272 2081 47
StuI AGG'CCT
Cuts at: 1517 2214 3219 3458 1517
Size: 697 1005 239 4459
Fragments arranged by size:
4459 1005 697 239
StyI C'CwwG_G
Cuts at: 513 1065 2868 3171 3839 513
Size: 552 1803 303 668 3074
Fragments arranged by size:
3074 1803 668 552 303
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3374 3475 4427 4444 4597 4782 5227
Size: 1255 1794 101 952 17 153 185 445
Cuts at: 5227 325
Size: 1498
Fragments arranged by size:
1794 1498 1255 952 445 185 153 101
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3114 4646 6358
Size: 274 80 33 51 1443 1137 1532 1712
Cuts at: 6358 96
Size: 138
Fragments arranged by size:
1712 1532 1443 1137 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3467 3499 6360 6392 3467
Size: 32 2861 32 3475
Fragments arranged by size:
3475 2861 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6400
XbaI T'CTAG_A
Cuts at: 3736 3736
Size: 6400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 3163 3169 2875
Size: 288 6 6106
Fragments arranged by size:
6106 288 6
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 2542 2551 3725 1870
Size: 672 9 1174 4545
Fragments arranged by size:
4545 1174 672 9
XmnI GAAnn'nnTTC
Cuts at: 2384 4529 2384
Size: 2145 4255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SwaI