Restriction map for
pFN21AE1986
(Circular) (Six-base) MAPSORT of: pFN21AE1986.seq Check: 9557 from: 1 to: 6355
pFN21AE1986 6355 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2660 2660
Size: 6355
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3698 1937
Size: 1761 4594
Acc65I G'GTAC_C
Cuts at: 3092 3676 3092
Size: 584 5771
AclI AA'CG_TT
Cuts at: 1563 4482 4855 1563
Size: 2919 373 3063
Fragments arranged by size:
3063 2919 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4418 5802 6070 1632
Size: 2786 1384 268 1917
Fragments arranged by size:
2786 1917 1384 268
AfeI AGC'GCT
Cuts at: 3296 3296
Size: 6355
AflII C'TTAA_G
Cuts at: 820 1017 2986 820
Size: 197 1969 4189
Fragments arranged by size:
4189 1969 197
AflIII A'CryG_T
Cuts at: 1203 2865 5254 6231 1203
Size: 1662 2389 977 1327
Fragments arranged by size:
2389 1662 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 5084 5084
Size: 6355
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6355
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6355
AlwNI CAG_nnn'CTG
Cuts at: 2096 2587 4098 5670 2096
Size: 491 1511 1572 2781
Fragments arranged by size:
2781 1572 1511 491
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2573 2642 3664 3890 4042 6151 1493
Size: 1080 69 1022 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 1022 226 152 69
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3680 1870
Size: 73 1737 4545
Fragments arranged by size:
4545 1737 73
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2125 2228 3092 3676 5131
Size: 325 217 627 338 103 864 584 1455
Cuts at: 5131 618
Size: 1842
Fragments arranged by size:
1842 1455 864 627 584 338 325 217
103
BanII G_rGCy'C
Cuts at: 1391 2985 3545 3674 1391
Size: 1594 560 129 4072
Fragments arranged by size:
4072 1594 560 129
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2025 3028 928
Size: 971 126 1003 4255
Fragments arranged by size:
4255 1003 971 126
BbvCI CC'TCA_GC
Cuts at: 2086 2086
Size: 6355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2444 2591 4247 5457 2444
Size: 147 1656 1210 3342
Fragments arranged by size:
3342 1656 1210 147
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6355
BfrBI ATG'CAT
Cuts at: 2731 2731
Size: 6355
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4966 136
Size: 107 122 71 631 605 3294 1525
Fragments arranged by size:
3294 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 4416 5572 1790
Size: 2626 1156 2573
Fragments arranged by size:
2626 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2328 3555 4086
Size: 416 53 83 399 24 911 1227 531
Cuts at: 4086 5044 442
Size: 958 1753
Fragments arranged by size:
1753 1227 958 911 531 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2517 3376 5015 1239
Size: 1278 859 1639 2579
Fragments arranged by size:
2579 1639 1278 859
Bpu10I CC'TnA_GC
Cuts at: 2086 2086
Size: 6355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4480 5345 5643 4480
Size: 865 298 5192
Fragments arranged by size:
5192 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2101 3433 5018 882
Size: 617 602 1332 1585 2219
Fragments arranged by size:
2219 1585 1332 617 602
BsaAI yAC'GTr
Cuts at: 493 1204 2311 4146 493
Size: 711 1107 1835 2702
Fragments arranged by size:
2702 1835 1107 711
BsaBI GATnn'nnATC
Cuts at: 2951 6349 2951
Size: 3398 2957
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 4162 4544 5179
Size: 53 83 186 936 476 2153 382 635
Cuts at: 5179 275
Size: 1451
Fragments arranged by size:
2153 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2254 2284 3237 3267 1178
Size: 30 165 30 851 30 953 30 4266
Fragments arranged by size:
4266 953 851 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2008 1186
Size: 822 5533
BseYI C'CCAG_C
Cuts at: 5558 6054 5558
Size: 496 5859
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 1163
Size: 573 948 4834
Fragments arranged by size:
4834 948 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 3805 4566 5594 1540
Size: 275 186 39 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186 39
BsiHKAI G_wGCw'C
Cuts at: 3219 4416 4501 5572 3219
Size: 1197 85 1071 4002
Fragments arranged by size:
4002 1197 1071 85
BsmI GAATG_Cn'
Cuts at: 3866 3959 3866
Size: 93 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2985 3219 3545 3674 4416 4501 5572
Size: 399 1195 234 326 129 742 85 1071
Cuts at: 5572 1391
Size: 2174
Fragments arranged by size:
2174 1195 1071 742 399 326 234 129
85
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 4244 5974 4244
Size: 1730 4625
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2660 3710 844
Size: 1816 1050 3489
Fragments arranged by size:
3489 1816 1050
BsrBI CCG'CTC
Cuts at: 1394 1823 4242 1394
Size: 429 2419 3507
Fragments arranged by size:
3507 2419 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4850 5024 65
Size: 1183 3602 174 1396
Fragments arranged by size:
3602 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3025 4999 5186 1700
Size: 110 1215 1974 187 2869
Fragments arranged by size:
2869 1974 1215 187 110
BsrGI T'GTAC_A
Cuts at: 96 2868 96
Size: 2772 3583
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2402 2587 3405 794
Size: 1608 185 818 3744
Fragments arranged by size:
3744 1608 818 185
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 3058 3119 3058
Size: 61 6294
BstYI r'GATC_y
Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350
Size: 1713 367 385 17 1452 86 12 346
Cuts at: 6350 1972
Size: 1977
Fragments arranged by size:
1977 1713 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2182 2182
Size: 6355
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2112 3057 3096 513
Size: 552 939 57 51 945 39 3772
Fragments arranged by size:
3772 945 939 552 57 51 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3248 487
Size: 683 56 2022 3594
Fragments arranged by size:
3594 2022 683 56
BtsI GCAGTG_nn'
Cuts at: 2473 2998 3871 4665 4685 2473
Size: 525 873 794 20 4143
Fragments arranged by size:
4143 873 794 525 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 2812 3659 2812
Size: 847 5508
DraIII CAC_nnn'GTG
Cuts at: 2257 2257
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3802 4691 6320
Size: 54 1425 50 275 18 1972 889 1629
Cuts at: 6320 8
Size: 43
Fragments arranged by size:
1972 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 4285 1353
Size: 1452 1480 3423
Fragments arranged by size:
3423 1480 1452
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2585 4942 5316 5462 387
Size: 1064 138 996 2357 374 146 1280
Fragments arranged by size:
2357 1280 1064 996 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2517 3376 4418 5015 5802 6070 1239
Size: 393 885 859 1042 597 787 268 1524
Fragments arranged by size:
1524 1042 885 859 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 6355
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 3298 5502 1678
Size: 1620 2204 2531
Fragments arranged by size:
2531 2204 1620
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 2689 2721 3237
Size: 32 135 32 772 32 313 32 516
Cuts at: 3237 3239 3269 3271 4996 5028 5070 5102 1373
Size: 2 30 2 1725 32 42 32 2626
Fragments arranged by size:
2626 1725 772 516 313 135 42 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3623 748
Size: 2875 3480
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3096 3680 3096
Size: 584 5771
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2581 3192 3559 5469 5653 1305
Size: 1276 611 367 1910 184 2007
Fragments arranged by size:
2007 1910 1276 611 367 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4533
Fragments arranged by size:
4533 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 2370 3056 3117 4313 4672 4831
Size: 648 1003 201 686 61 1196 359 159
Cuts at: 4831 518
Size: 2042
Fragments arranged by size:
2042 1196 1003 686 648 359 201 159
61
MspA1I CmG'CkG
Cuts at: 1606 2063 2476 3771 4448 5596 5841 1606
Size: 457 413 1295 677 1148 245 2120
Fragments arranged by size:
2120 1295 1148 677 457 413 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2112 3057 3096 513
Size: 552 939 108 945 39 3772
Fragments arranged by size:
3772 945 939 552 108 39
NdeI CA'TA_TG
Cuts at: 387 3114 387
Size: 2727 3628
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NsiI A_TGCA'T
Cuts at: 2733 2733
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2146 2869 3713 5258 1132
Size: 97 917 723 844 1545 2229
Fragments arranged by size:
2229 1545 917 844 723 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 2865 5254 2865
Size: 2389 3966
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 5206
PfoI T'CCnGG_A
Cuts at: 2418 2593 2418
Size: 175 6180
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 4424 4456 5937 5969 3629
Size: 32 763 32 1481 32 4015
Fragments arranged by size:
4015 1481 763 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PstI C_TGCA'G
Cuts at: 830 2468 2583 2895 2916 3707 830
Size: 1638 115 312 21 791 3478
Fragments arranged by size:
3478 1638 791 312 115 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 3005 4603 6315 3005
Size: 1598 1712 3045
Fragments arranged by size:
3045 1712 1598
SfcI C'TryA_G
Cuts at: 826 1045 2464 2488 2579 2891 2912 3185 3703
Size: 219 1419 24 91 312 21 273 518
Cuts at: 3703 4838 5519 5710 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1419 1135 681 518 312 273 219
191 91 24 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2986 4459 5360 5622 820
Size: 197 926 1043 1473 901 262 1553
Fragments arranged by size:
1553 1473 1043 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 2146 3713 1132
Size: 1014 1567 3774
Fragments arranged by size:
3774 1567 1014
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6355
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 3057 3096 3487 3615 3794
Size: 552 939 108 945 39 391 128 179
Cuts at: 3794 513
Size: 3074
Fragments arranged by size:
3074 945 939 552 391 179 128 108
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 3107 3348 3415 3665 4382 4399
Size: 1255 446 1081 241 67 250 717 17
Cuts at: 4399 4552 4737 5182 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1081 717 446 445 250 241
185 153 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 2868 3003
Size: 274 80 33 51 1443 678 213 135
Cuts at: 3003 3608 4601 6313 96
Size: 605 993 1712 138
Fragments arranged by size:
1712 1443 993 678 605 274 213 138
135 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6315 6347 6315
Size: 32 6323
Tth111I GACn'n_nGTC
Cuts at: 1316 3251 1316
Size: 1935 4420
XbaI T'CTAG_A
Cuts at: 3691 3691
Size: 6355
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 3153 4484 3153
Size: 1331 5024
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI