Restriction map for
pFN21AE1984
(Circular) (Six-base) MAPSORT of: pFN21AE1984.seq Check: 6367 from: 1 to: 6382
pFN21AE1984 6382 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3725 1937
Size: 1788 4594
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2993 3391 4445 5829 6097 1632
Size: 1361 398 1054 1384 268 1917
Fragments arranged by size:
1917 1384 1361 1054 398 268
AflII C'TTAA_G
Cuts at: 820 1017 2251 820
Size: 197 1234 4951
Fragments arranged by size:
4951 1234 197
AflIII A'CryG_T
Cuts at: 1203 3578 5281 6258 1203
Size: 2375 1703 977 1327
Fragments arranged by size:
2375 1703 1327 977
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 2658 3637 1166
Size: 1492 979 3911
Fragments arranged by size:
3911 1492 979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6382
AlwNI CAG_nnn'CTG
Cuts at: 4125 5697 4125
Size: 1572 4810
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 2168 2537 2846 2923 3691 3917 4069 6178
Size: 675 369 309 77 768 226 152 2109
Cuts at: 6178 1493
Size: 1697
Fragments arranged by size:
2109 1697 768 675 369 309 226 152
77
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 3707 1870
Size: 73 1764 4545
Fragments arranged by size:
4545 1764 73
BamHI G'GATC_C
Cuts at: 2058 3712 4079 2058
Size: 1654 367 4361
Fragments arranged by size:
4361 1654 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3703 5158 618
Size: 325 217 627 1916 1455 1842
Fragments arranged by size:
1916 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2723 3701 1391
Size: 1332 978 4072
Fragments arranged by size:
4072 1332 978
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2289 928
Size: 971 390 5021
Fragments arranged by size:
5021 971 390
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 1903
Size: 34 2636 34 3678
Fragments arranged by size:
3678 2636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3526 4274 5484 3526
Size: 748 1210 4424
Fragments arranged by size:
4424 1210 748
BclI T'GATC_A
Cuts at: 1548 2185 2350 1548
Size: 637 165 5580
Fragments arranged by size:
5580 637 165
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4993 136
Size: 107 122 71 631 605 3321 1525
Fragments arranged by size:
3321 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 4443 5599 1790
Size: 2653 1156 2573
Fragments arranged by size:
2653 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6382
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4113 5071 442
Size: 416 53 83 399 24 2696 958 1753
Fragments arranged by size:
2696 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3615 3615
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2034 2145 2400 5042 1239
Size: 795 111 255 2642 2579
Fragments arranged by size:
2642 2579 795 255 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3060 3089 3585 4507 5372 5670 3060
Size: 29 496 922 865 298 3772
Fragments arranged by size:
3772 922 865 496 298 29
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3000 3329 5045 882
Size: 617 1501 329 1716 2219
Fragments arranged by size:
2219 1716 1501 617 329
BsaAI yAC'GTr
Cuts at: 493 1204 4173 493
Size: 711 2969 2702
Fragments arranged by size:
2969 2702 711
BsaBI GATnn'nnATC
Cuts at: 2232 6376 2232
Size: 4144 2238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4189 4571 5206 275
Size: 53 83 186 936 2656 382 635 1451
Fragments arranged by size:
2656 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4815 5213 5487 5634 6045 4815
Size: 398 274 147 411 5152
Fragments arranged by size:
5152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6157
Fragments arranged by size:
6157 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6382
BseYI C'CCAG_C
Cuts at: 5585 6081 5585
Size: 496 5886
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5809
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3832 4593 5621 1540
Size: 275 186 1831 761 1028 2301
Fragments arranged by size:
2301 1831 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4443 4528 5599 4443
Size: 85 1071 5226
Fragments arranged by size:
5226 1071 85
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2723 3701 4443 4528 5599 1391
Size: 399 933 978 742 85 1071 2174
Fragments arranged by size:
2174 1071 978 933 742 399 85
BspEI T'CCGG_A
Cuts at: 6045 6045
Size: 6382
BspHI T'CATG_A
Cuts at: 2353 4271 6001 2353
Size: 1918 1730 2734
Fragments arranged by size:
2734 1918 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3737 844
Size: 2893 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2471 3399 4877 5051 65
Size: 1183 1223 928 1478 174 1396
Fragments arranged by size:
1478 1396 1223 1183 928 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3355 5026 5213 1700
Size: 110 1545 1671 187 2869
Fragments arranged by size:
2869 1671 1545 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6382
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 4442 5454 4442
Size: 1012 5370
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 5186 5186
Size: 6382
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstYI r'GATC_y
Cuts at: 1972 2058 3712 4079 4464 4481 5933 6019 6031
Size: 86 1654 367 385 17 1452 86 12
Cuts at: 6031 6377 1972
Size: 346 1977
Fragments arranged by size:
1977 1654 1452 385 367 346 86 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3135 3187 513
Size: 552 939 1131 52 3708
Fragments arranged by size:
3708 1131 939 552 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2469 487
Size: 683 56 1243 4400
Fragments arranged by size:
4400 1243 683 56
BtsI GCAGTG_nn'
Cuts at: 3460 3898 4692 4712 3460
Size: 438 794 20 5130
Fragments arranged by size:
5130 794 438 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6347
DraI TTT'AAA
Cuts at: 2070 2140 3686 2070
Size: 70 1546 4766
Fragments arranged by size:
4766 1546 70
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2476 3214 5389 809
Size: 507 1160 738 2175 1802
Fragments arranged by size:
2175 1802 1160 738 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3829 4718 6347
Size: 54 1425 50 275 18 1999 889 1629
Cuts at: 6347 8
Size: 43
Fragments arranged by size:
1999 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3829 1537
Size: 275 2017 4090
Fragments arranged by size:
4090 2017 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2301 2487 4312 1353
Size: 948 186 1825 3423
Fragments arranged by size:
3423 1825 948 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4969 5343 5489 387
Size: 1064 138 3380 374 146 1280
Fragments arranged by size:
3380 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2034 2145 2400 2993 3391 4445 5042
Size: 393 402 111 255 593 398 1054 597
Cuts at: 5042 5829 6097 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1054 787 597 593 402 398 393
268 255 111
EcoRI G'AATT_C
Cuts at: 3691 4069 3691
Size: 378 6004
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 5529 1678
Size: 3851 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2926 2929 2958 2960
Size: 32 135 32 1325 29 3 29 2
Cuts at: 2960 2992 3110 3142 3581 3613 5023 5055 5097
Size: 32 118 32 439 32 1410 32 42
Cuts at: 5097 5129 1373
Size: 32 2626
Fragments arranged by size:
2626 1410 1325 439 135 118 42 32
32 32 32 32 32 32 29 29
3 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3726 3972 669
Size: 988 281 1788 246 3079
Fragments arranged by size:
3079 1788 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2241 748
Size: 1493 4889
HpaI GTT'AAC
Cuts at: 3972 3972
Size: 6382
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 2841 3981 2841
Size: 1140 5242
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2401 2643 3623 5496 5680 1305
Size: 1096 242 980 1873 184 2007
Fragments arranged by size:
2007 1873 1096 980 242 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4560
Fragments arranged by size:
4560 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2658 3485 3577 3637 4340 4699 4858
Size: 648 1492 827 92 60 703 359 159
Cuts at: 4858 518
Size: 2042
Fragments arranged by size:
2042 1492 827 703 648 359 159 92
60
MspA1I CmG'CkG
Cuts at: 1606 3798 4475 5623 5868 1606
Size: 2192 677 1148 245 2120
Fragments arranged by size:
2192 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3135 3187 513
Size: 552 939 1131 52 3708
Fragments arranged by size:
3708 1131 939 552 52
NdeI CA'TA_TG
Cuts at: 387 3426 387
Size: 3039 3343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3436 3582 3740 5285 1132
Size: 97 2207 146 158 1545 2229
Fragments arranged by size:
2229 2207 1545 158 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 3578 5281 3578
Size: 1703 4679
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6382
PfoI T'CCnGG_A
Cuts at: 2050 2554 2641 2050
Size: 504 87 5791
Fragments arranged by size:
5791 504 87
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2103 2135 4451 4483 5964 5996 2103
Size: 32 2316 32 1481 32 2489
Fragments arranged by size:
2489 2316 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PstI C_TGCA'G
Cuts at: 830 3734 830
Size: 2904 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 4630 6342 4630
Size: 1712 4670
SfcI C'TryA_G
Cuts at: 826 1045 2208 3318 3730 4865 5546 5737 826
Size: 219 1163 1110 412 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 1110 681 412 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 3709 1872
Size: 1837 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2251 3068 3075 3600 4486 5387
Size: 197 926 308 817 7 525 886 901
Cuts at: 5387 5649 820
Size: 262 1553
Fragments arranged by size:
1553 926 901 886 817 525 308 262
197 7
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 2804 152
Size: 2652 3730
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SspI AAT'ATT
Cuts at: 5 52 3106 3526 4306 5
Size: 47 3054 420 780 2081
Fragments arranged by size:
3054 2081 780 420 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6382
StyI C'CwwG_G
Cuts at: 513 1065 2004 2342 3135 3187 3821 513
Size: 552 939 338 793 52 634 3074
Fragments arranged by size:
3074 939 793 634 552 338 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2842 4409 4426 4579 4764 5209 325
Size: 1255 1262 1567 17 153 185 445 1498
Fragments arranged by size:
1567 1498 1262 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4628 6340 96
Size: 274 80 33 51 1443 2651 1712 138
Fragments arranged by size:
2651 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6342 6374 6342
Size: 32 6350
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2988 2988
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6382
XmaI C'CCGG_G
Cuts at: 1870 3707 1870
Size: 1837 4545
XmnI GAAnn'nnTTC
Cuts at: 2229 2923 4511 2229
Size: 694 1588 4100
Fragments arranged by size:
4100 1588 694
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI