Restriction map for
pFN21AE1978
(Circular) (Six-base) MAPSORT of: pFN21AE1978.seq Check: 7286 from: 1 to: 6370
pFN21AE1978 6370 bp
With 182 enzymes: SgfI *
June 30, 2019 15:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2228 2735 3611 2228
Size: 507 876 4987
Fragments arranged by size:
4987 876 507
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4180 278
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3713 1937
Size: 1776 4594
Acc65I G'GTAC_C
Cuts at: 3691 3691
Size: 6370
AclI AA'CG_TT
Cuts at: 1563 4497 4870 1563
Size: 2934 373 3063
Fragments arranged by size:
3063 2934 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2367 2494 4433 5817 6085 1632
Size: 735 127 1939 1384 268 1917
Fragments arranged by size:
1939 1917 1384 735 268 127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6173
AflIII A'CryG_T
Cuts at: 1203 5269 6246 1203
Size: 4066 977 1327
Fragments arranged by size:
4066 1327 977
AgeI A'CCGG_T
Cuts at: 5201 5201
Size: 6370
AhdI GACnn_n'nnGTC
Cuts at: 5099 5099
Size: 6370
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6370
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6370
AlwNI CAG_nnn'CTG
Cuts at: 2266 3106 3346 4113 5685 2266
Size: 840 240 767 1572 2951
Fragments arranged by size:
2951 1572 840 767 240
ApaI G_GGCC'C
Cuts at: 2296 2593 2970 3094 3435 2296
Size: 297 377 124 341 5231
Fragments arranged by size:
5231 377 341 297 124
ApaLI G'TGCA_C
Cuts at: 4427 5583 4427
Size: 1156 5214
ApoI r'AATT_y
Cuts at: 1493 3557 3679 3905 4057 6166 1493
Size: 2064 122 226 152 2109 1697
Fragments arranged by size:
2109 2064 1697 226 152 122
AseI AT'TA_AT
Cuts at: 160 1032 4924 160
Size: 872 3892 1606
Fragments arranged by size:
3892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
AvaI C'yCGr_G
Cuts at: 1870 1943 2170 2358 2594 2969 3171 3695 1870
Size: 73 227 188 236 375 202 524 4545
Fragments arranged by size:
4545 524 375 236 227 202 188 73
BamHI G'GATC_C
Cuts at: 2955 3700 4067 2955
Size: 745 367 5258
Fragments arranged by size:
5258 745 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2216 2252 2663 3488 3599
Size: 325 217 627 429 36 411 825 111
Cuts at: 3599 3691 5146 618
Size: 92 1455 1842
Fragments arranged by size:
1842 1455 825 627 429 411 325 217
111 92 36
BanII G_rGCy'C
Cuts at: 1391 2177 2296 2303 2593 2751 2970 3094 3121
Size: 786 119 7 290 158 219 124 27
Cuts at: 3121 3170 3435 3689 1391
Size: 49 265 254 4072
Fragments arranged by size:
4072 786 290 265 254 219 158 124
119 49 27 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2492 928
Size: 971 593 4806
Fragments arranged by size:
4806 971 593
BbvCI CC'TCA_GC
Cuts at: 3347 3347
Size: 6370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3162 3196 4561 4595 1903
Size: 34 1225 34 1365 34 3678
Fragments arranged by size:
3678 1365 1225 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4262 5472 4262
Size: 1210 5160
BclI T'GATC_A
Cuts at: 1548 3496 1548
Size: 1948 4422
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2172 4981 136
Size: 107 122 71 631 605 500 2809 1525
Fragments arranged by size:
2809 1525 631 605 500 122 107 71
BglII A'GATC_T
Cuts at: 6365 6365
Size: 6370
BlpI GC'TnA_GC
Cuts at: 3202 3787 3202
Size: 585 5785
Bme1580I G_kGCm'C
Cuts at: 1790 2255 2296 2593 2970 3094 3149 3435 4431
Size: 465 41 297 377 124 55 286 996
Cuts at: 4431 5587 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 996 465 377 297 286 124
55 41
BmgBI CAC'GTC
Cuts at: 1318 2152 2407 1318
Size: 834 255 5281
Fragments arranged by size:
5281 834 255
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 4101 5059
Size: 416 53 83 399 24 760 1924 958
Cuts at: 5059 442
Size: 1753
Fragments arranged by size:
1924 1753 958 760 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 2995
Size: 6370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2243 3145 3275 3627 5030 1239
Size: 1004 902 130 352 1403 2579
Fragments arranged by size:
2579 1403 1004 902 352 130
Bpu10I CC'TnA_GC
Cuts at: 2415 3347 2415
Size: 932 5438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4495 5360 5658 4495
Size: 865 298 5207
Fragments arranged by size:
5207 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3168 5033 882
Size: 617 1669 1865 2219
Fragments arranged by size:
2219 1865 1669 617
BsaAI yAC'GTr
Cuts at: 493 1204 3046 4161 493
Size: 711 1842 1115 2702
Fragments arranged by size:
2702 1842 1115 711
BsaBI GATnn'nnATC
Cuts at: 6364 6364
Size: 6370
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4177 4559 5194 275
Size: 53 83 186 936 2644 382 635 1451
Fragments arranged by size:
2644 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3651 4803 5201 5475 5622 6033 3651
Size: 1152 398 274 147 411 3988
Fragments arranged by size:
3988 1152 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6145
Fragments arranged by size:
6145 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2858 3100 3609 1186
Size: 826 846 242 509 3947
Fragments arranged by size:
3947 846 826 509 242
BseYI C'CCAG_C
Cuts at: 2628 2689 3249 5573 6069 2628
Size: 61 560 2324 496 2929
Fragments arranged by size:
2929 2324 560 496 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2206 2555 3026 1163
Size: 573 470 349 471 4507
Fragments arranged by size:
4507 573 471 470 349
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3143 3820 4581 5609 1540
Size: 275 186 1142 677 761 1028 2301
Fragments arranged by size:
2301 1142 1028 761 677 275 186
BsiHKAI G_wGCw'C
Cuts at: 3359 3417 4431 4516 5587 3359
Size: 58 1014 85 1071 4142
Fragments arranged by size:
4142 1071 1014 85 58
BsmI GAATG_Cn'
Cuts at: 3881 3974 3881
Size: 93 6277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2255 2296 2303 2593 2751 2970
Size: 399 387 78 41 7 290 158 219
Cuts at: 2970 3094 3121 3149 3170 3359 3417 3435 3689
Size: 124 27 28 21 189 58 18 254
Cuts at: 3689 4431 4516 5587 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 387 290 254 219
189 158 124 85 78 58 41 28
27 21 18 7
BspEI T'CCGG_A
Cuts at: 6033 6033
Size: 6370
BspHI T'CATG_A
Cuts at: 4259 5989 4259
Size: 1730 4640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2228 2610 2735 2952 3436 3611 3725 844
Size: 1384 382 125 217 484 175 114 3489
Fragments arranged by size:
3489 1384 484 382 217 175 125 114
BsrBI CCG'CTC
Cuts at: 1394 1823 2169 4257 1394
Size: 429 346 2088 3507
Fragments arranged by size:
3507 2088 429 346
BsrDI GCAATG_nn'
Cuts at: 65 1248 2434 4865 5039 65
Size: 1183 1186 2431 174 1396
Fragments arranged by size:
2431 1396 1186 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2447 3138 5014 5201 1700
Size: 110 637 691 1876 187 2869
Fragments arranged by size:
2869 1876 691 637 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6370
BssSI C'ACGA_G
Cuts at: 4430 5442 4430
Size: 1012 5358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3049 794
Size: 2255 4115
BstBI TT'CG_AA
Cuts at: 5174 5174
Size: 6370
BstEII G'GTnAC_C
Cuts at: 1184 3208 3259 1184
Size: 2024 51 4295
Fragments arranged by size:
4295 2024 51
BstXI CCAn_nnnn'nTGG
Cuts at: 2303 2303
Size: 6370
BstYI r'GATC_y
Cuts at: 1972 2955 3700 4067 4452 4469 5921 6007 6019
Size: 983 745 367 385 17 1452 86 12
Cuts at: 6019 6365 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 983 745 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2642 3437 2642
Size: 795 5575
BtgI C'CryG_G
Cuts at: 513 1065 2004 2809 3068 3196 3306 513
Size: 552 939 805 259 128 110 3577
Fragments arranged by size:
3577 939 805 552 259 128 110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5631
Fragments arranged by size:
5631 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 3300 3886 4680 4700 2712
Size: 588 586 794 20 4382
Fragments arranged by size:
4382 794 588 586 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6370
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6335
DraI TTT'AAA
Cuts at: 3674 3674
Size: 6370
DraIII CAC_nnn'GTG
Cuts at: 3363 3363
Size: 6370
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2393 5377 809
Size: 507 1077 2984 1802
Fragments arranged by size:
2984 1802 1077 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2445 2695
Size: 54 1425 50 275 18 303 312 250
Cuts at: 2695 2920 3140 3337 3420 3547 3817 4706 6335
Size: 225 220 197 83 127 270 889 1629
Cuts at: 6335 8
Size: 43
Fragments arranged by size:
1629 1425 889 312 303 275 270 250
225 220 197 127 83 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3140 3817 1537
Size: 275 1328 677 4090
Fragments arranged by size:
4090 1328 677 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3158 3332 4300 1353
Size: 1805 174 968 3423
Fragments arranged by size:
3423 1805 968 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2153 4957 5331 5477 387
Size: 1064 138 564 2804 374 146 1280
Fragments arranged by size:
2804 1280 1064 564 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2243 2367 2494 3145 3275 3627 4433
Size: 393 611 124 127 651 130 352 806
Cuts at: 4433 5030 5817 6085 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 806 787 651 611 597 393 352
268 130 127 124
EcoNI CCTnn'n_nnAGG
Cuts at: 2246 2641 2246
Size: 395 5975
EcoO109I rG'GnC_Cy
Cuts at: 2589 2590 2599 3064 3090 3091 3241 3280 3431
Size: 1 9 465 26 1 150 39 151
Cuts at: 3431 3432 2589
Size: 1 5527
Fragments arranged by size:
5527 465 151 150 39 26 9 1
1 1
EcoRI G'AATT_C
Cuts at: 3679 4057 3679
Size: 378 5992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3346 3346
Size: 6370
FspI TGC'GCA
Cuts at: 2723 4876 2723
Size: 2153 4217
HaeII r_GCGC'y
Cuts at: 1678 2266 2279 2317 2902 5517 1678
Size: 588 13 38 585 2615 2531
Fragments arranged by size:
2615 2531 588 585 38 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3446 3478 5011 5043 5085
Size: 32 135 32 1874 32 1533 32 42
Cuts at: 5085 5117 1373
Size: 32 2626
Fragments arranged by size:
2626 1874 1533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2797 3714 3960 669
Size: 988 281 859 917 246 3079
Fragments arranged by size:
3079 988 917 859 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6370
HpaI GTT'AAC
Cuts at: 3960 3960
Size: 6370
KpnI G_GTAC'C
Cuts at: 3695 3695
Size: 6370
MfeI C'AATT_G
Cuts at: 3969 3969
Size: 6370
MluI A'CGCG_T
Cuts at: 6246 6246
Size: 6370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 5484 5668 1305
Size: 1374 2805 184 2007
Fragments arranged by size:
2805 2007 1374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3339 3422 3549 10
Size: 54 1425 343 1507 83 127 2831
Fragments arranged by size:
2831 1507 1425 343 127 83 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3184 4328 4687 4846 518
Size: 648 2018 1144 359 159 2042
Fragments arranged by size:
2042 2018 1144 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2569 3070 3206 3253 3786 4463 5611 5856
Size: 963 501 136 47 533 677 1148 245
Cuts at: 5856 1606
Size: 2120
Fragments arranged by size:
2120 1148 963 677 533 501 245 136
47
NaeI GCC'GGC
Cuts at: 2449 3140 2449
Size: 691 5679
NcoI C'CATG_G
Cuts at: 513 1065 2004 3196 513
Size: 552 939 1192 3687
Fragments arranged by size:
3687 1192 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6370
NgoMIV G'CCGG_C
Cuts at: 2447 3138 2447
Size: 691 5679
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6370
NotI GC'GGCC_GC
Cuts at: 3817 3817
Size: 6370
NspI r_CATG'y
Cuts at: 1132 1229 2438 3728 5273 1132
Size: 97 1209 1290 1545 2229
Fragments arranged by size:
2229 1545 1290 1209 97
PasI CC'CwG_GG
Cuts at: 1348 2296 1348
Size: 948 5422
PciI A'CATG_T
Cuts at: 5269 5269
Size: 6370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6370
PfoI T'CCnGG_A
Cuts at: 3643 3643
Size: 6370
PmeI GTTT'AAAC
Cuts at: 3674 3674
Size: 6370
PmlI CAC'GTG
Cuts at: 3046 3046
Size: 6370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4439 4471 5952 5984 4439
Size: 32 1481 32 4825
Fragments arranged by size:
4825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2599 3064 2599
Size: 465 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6370
PsiI TTA'TAA
Cuts at: 3940 3940
Size: 6370
PspOMI G'GGCC_C
Cuts at: 2292 2589 2966 3090 3431 2292
Size: 297 377 124 341 5231
Fragments arranged by size:
5231 377 341 297 124
PstI C_TGCA'G
Cuts at: 830 3137 3722 830
Size: 2307 585 3478
Fragments arranged by size:
3478 2307 585
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6370
PvuII CAG'CTG
Cuts at: 3253 3253
Size: 6370
SacII CC_GC'GG
Cuts at: 3071 3071
Size: 6370
SalI G'TCGA_C
Cuts at: 1936 3712 1936
Size: 1776 4594
SapI GCTCTTCn'nnn_
Cuts at: 3158 3332 3158
Size: 174 6196
SbfI CC_TGCA'GG
Cuts at: 3722 3722
Size: 6370
ScaI AGT'ACT
Cuts at: 2978 4618 6330 2978
Size: 1640 1712 3018
Fragments arranged by size:
3018 1712 1640
SfcI C'TryA_G
Cuts at: 826 1045 2243 2539 3133 3718 4853 5534 5725
Size: 219 1198 296 594 585 1135 681 191
Cuts at: 5725 826
Size: 1471
Fragments arranged by size:
1471 1198 1135 681 594 585 296 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6370
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6370
SmaI CCC'GGG
Cuts at: 1872 2360 3697 1872
Size: 488 1337 4545
Fragments arranged by size:
4545 1337 488
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4474 5375 5637 820
Size: 197 926 2531 901 262 1553
Fragments arranged by size:
2531 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4161 493
Size: 3668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6370
SphI G_CATG'C
Cuts at: 1132 3728 1132
Size: 2596 3774
SspI AAT'ATT
Cuts at: 5 52 4294 5
Size: 47 4242 2081
Fragments arranged by size:
4242 2081 47
StuI AGG'CCT
Cuts at: 1517 2837 1517
Size: 1320 5050
StyI C'CwwG_G
Cuts at: 513 1065 2004 2832 3196 3484 3809 513
Size: 552 939 828 364 288 325 3074
Fragments arranged by size:
3074 939 828 552 364 325 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2642 2681 3230 4397 4414 4567 4752
Size: 1255 1062 39 549 1167 17 153 185
Cuts at: 4752 5197 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1167 1062 549 445 185 153
39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2502 2976 4616
Size: 274 80 33 51 1443 525 474 1640
Cuts at: 4616 6328 96
Size: 1712 138
Fragments arranged by size:
1712 1640 1443 525 474 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 6330 6362 2184
Size: 32 4114 32 2192
Fragments arranged by size:
4114 2192 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3614 1316
Size: 2298 4072
XbaI T'CTAG_A
Cuts at: 3706 3706
Size: 6370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 6370
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6370
XmaI C'CCGG_G
Cuts at: 1870 2358 3695 1870
Size: 488 1337 4545
Fragments arranged by size:
4545 1337 488
XmnI GAAnn'nnTTC
Cuts at: 3326 4499 3326
Size: 1173 5197
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4178 276
Size: 53 83 186 936 2644 2468
Fragments arranged by size:
2644 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI