Restriction map for
pFN21AE1971
(Circular) (Six-base) MAPSORT of: pFN21AE1971.seq Check: 3537 from: 1 to: 6319
pFN21AE1971 6319 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3286 3662 1937
Size: 1349 376 4594
Fragments arranged by size:
4594 1349 376
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2173 2387 2435 2717 3534 4382 5766 6034
Size: 541 214 48 282 817 848 1384 268
Cuts at: 6034 1632
Size: 1917
Fragments arranged by size:
1917 1384 848 817 541 282 268 214
48
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 5218 6195 1203
Size: 4015 977 1327
Fragments arranged by size:
4015 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6319
AlwNI CAG_nnn'CTG
Cuts at: 3466 4062 5634 3466
Size: 596 1572 4151
Fragments arranged by size:
4151 1572 596
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 2472 2720 2765 3628 3854 4006 6115 1493
Size: 979 248 45 863 226 152 2109 1697
Fragments arranged by size:
2109 1697 979 863 248 226 152 45
AseI AT'TA_AT
Cuts at: 160 1032 2388 4873 160
Size: 872 1356 2485 1606
Fragments arranged by size:
2485 1606 1356 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 3483 3644 1870
Size: 73 934 606 161 4545
Fragments arranged by size:
4545 934 606 161 73
BamHI G'GATC_C
Cuts at: 3649 4016 3649
Size: 367 5952
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2134 3640 5095 618
Size: 325 217 627 347 1506 1455 1842
Fragments arranged by size:
1842 1506 1455 627 347 325 217
BanII G_rGCy'C
Cuts at: 1391 2470 3638 1391
Size: 1079 1168 4072
Fragments arranged by size:
4072 1168 1079
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3598 928
Size: 971 1699 3649
Fragments arranged by size:
3649 1699 971
BbvCI CC'TCA_GC
Cuts at: 3573 3573
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4510 4544 1903
Size: 34 2573 34 3678
Fragments arranged by size:
3678 2573 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2876 3131 4211 5421 2876
Size: 255 1080 1210 3774
Fragments arranged by size:
3774 1210 1080 255
BclI T'GATC_A
Cuts at: 1548 2892 1548
Size: 1344 4975
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2233 6314 2233
Size: 4081 2238
BlpI GC'TnA_GC
Cuts at: 2088 3736 2088
Size: 1648 4671
Bme1580I G_kGCm'C
Cuts at: 1790 2677 2937 3218 4380 5536 1790
Size: 887 260 281 1162 1156 2573
Fragments arranged by size:
2573 1162 1156 887 281 260
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4050 5008 442
Size: 416 53 83 399 24 2633 958 1753
Fragments arranged by size:
2633 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2581 2581
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 4979 1239
Size: 1070 2670 2579
Fragments arranged by size:
2670 2579 1070
Bpu10I CC'TnA_GC
Cuts at: 2255 3573 2255
Size: 1318 5001
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2231 4444 5309 5607 2231
Size: 2213 865 298 2943
Fragments arranged by size:
2943 2213 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4982 882
Size: 617 3483 2219
Fragments arranged by size:
3483 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2095 4110 493
Size: 711 891 2015 2702
Fragments arranged by size:
2702 2015 891 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4126 4508 5143 275
Size: 53 83 186 936 2593 382 635 1451
Fragments arranged by size:
2593 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 1178
Size: 30 165 30 1368 30 4696
Fragments arranged by size:
4696 1368 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 2795 3323 1186
Size: 1261 348 528 4182
Fragments arranged by size:
4182 1261 528 348
BseYI C'CCAG_C
Cuts at: 3382 5522 6018 3382
Size: 2140 496 3683
Fragments arranged by size:
3683 2140 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3025 1163
Size: 573 1289 4457
Fragments arranged by size:
4457 1289 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 4380 4465 5536 2094
Size: 2286 85 1071 2877
Fragments arranged by size:
2877 2286 1071 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2470 2677 2937 3218 3638 4380
Size: 399 304 376 207 260 281 420 742
Cuts at: 4380 4465 5536 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 420 399 376 304 281
260 207 85
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2097 2604 3674 844
Size: 1253 507 1070 3489
Fragments arranged by size:
3489 1253 1070 507
BsrBI CCG'CTC
Cuts at: 1394 1823 2153 4206 1394
Size: 429 330 2053 3507
Fragments arranged by size:
3507 2053 429 330
BsrDI GCAATG_nn'
Cuts at: 65 1248 4814 4988 65
Size: 1183 3566 174 1396
Fragments arranged by size:
3566 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4963 5150 1700
Size: 110 3153 187 2869
Fragments arranged by size:
3153 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6319
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2233 3649 4016 4401 4418 5870 5956 5968
Size: 261 1416 367 385 17 1452 86 12
Cuts at: 5968 6314 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1416 385 367 346 261 86
17 12
BstZ17I GTA'TAC
Cuts at: 3287 3287
Size: 6319
BtgI C'CryG_G
Cuts at: 513 1065 2004 3130 513
Size: 552 939 1126 3702
Fragments arranged by size:
3702 1126 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2144 3490 3504 487
Size: 683 56 918 1346 14 3302
Fragments arranged by size:
3302 1346 918 683 56 14
BtsI GCAGTG_nn'
Cuts at: 3835 4629 4649 3835
Size: 794 20 5505
Fragments arranged by size:
5505 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2983 3018 529
Size: 35 2419 35 3830
Fragments arranged by size:
3830 2419 35 35
DraI TTT'AAA
Cuts at: 2176 3623 2176
Size: 1447 4872
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3390 3766 4655
Size: 54 1425 50 275 18 1560 376 889
Cuts at: 4655 6284 8
Size: 1629 43
Fragments arranged by size:
1629 1560 1425 889 376 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2014 2384 2618 3000 4249 1353
Size: 661 370 234 382 1249 3423
Fragments arranged by size:
3423 1249 661 382 370 234
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2173 2309 2387 2435 2717 3534 4382
Size: 393 541 136 78 48 282 817 848
Cuts at: 4382 4979 5766 6034 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 848 817 787 597 541 393 282
268 136 78 48
EcoO109I rG'GnC_Cy
Cuts at: 3172 3172
Size: 6319
EcoRI G'AATT_C
Cuts at: 2720 3628 4006 2720
Size: 908 378 5033
Fragments arranged by size:
5033 908 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2203 2320 2203
Size: 117 6202
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 5466 1678
Size: 3788 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2819 2851 3176
Size: 32 135 32 653 32 562 32 325
Cuts at: 3176 3208 3323 3355 4960 4992 5034 5066 1373
Size: 32 115 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 653 562 325 135 115 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3663 3909 669
Size: 988 281 1725 246 3079
Fragments arranged by size:
3079 1725 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 2631 5433 5617 1305
Size: 1017 309 2802 184 2007
Fragments arranged by size:
2802 2007 1017 309 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3392 10
Size: 54 1425 343 1560 2937
Fragments arranged by size:
2937 1560 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2615 2976 4277 4636 4795 518
Size: 648 1449 361 1301 359 159 2042
Fragments arranged by size:
2042 1449 1301 648 361 359 159
MspA1I CmG'CkG
Cuts at: 1606 3735 4412 5560 5805 1606
Size: 2129 677 1148 245 2120
Fragments arranged by size:
2129 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3130 513
Size: 552 939 1126 3702
Fragments arranged by size:
3702 1126 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 3677 5222 1132
Size: 97 2448 1545 2229
Fragments arranged by size:
2448 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6319
PciI A'CATG_T
Cuts at: 5218 5218
Size: 6319
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3085 1108
Size: 1977 4342
PfoI T'CCnGG_A
Cuts at: 2325 3360 2325
Size: 1035 5284
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PmlI CAC'GTG
Cuts at: 2095 2095
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3889 3889
Size: 6319
PspXI vC'TCGA_Gb
Cuts at: 3483 3483
Size: 6319
PstI C_TGCA'G
Cuts at: 830 3430 3671 830
Size: 2600 241 3478
Fragments arranged by size:
3478 2600 241
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SapI GCTCTTCn'nnn_
Cuts at: 2014 2384 3000 2014
Size: 370 616 5333
Fragments arranged by size:
5333 616 370
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SexAI A'CCwGG_T
Cuts at: 3437 3437
Size: 6319
SfcI C'TryA_G
Cuts at: 826 1045 2482 3426 3667 4802 5483 5674 826
Size: 219 1437 944 241 1135 681 191 1471
Fragments arranged by size:
1471 1437 1135 944 681 241 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2246 3483 4423 5324 5586 820
Size: 197 926 303 1237 940 901 262 1553
Fragments arranged by size:
1553 1237 940 926 901 303 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6319
StyI C'CwwG_G
Cuts at: 513 1065 2004 3130 3758 513
Size: 552 939 1126 628 3074
Fragments arranged by size:
3074 1126 939 628 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4346 4363 4516 4701 5146 325
Size: 1255 2766 17 153 185 445 1498
Fragments arranged by size:
2766 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4565 6277 96
Size: 274 80 33 51 1443 2588 1712 138
Fragments arranged by size:
2588 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2771 2803 6279 6311 2771
Size: 32 3476 32 2779
Fragments arranged by size:
3476 2779 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2575
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 3483 1943
Size: 1540 4779
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 3009 4448 3009
Size: 1439 4880
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PstI PvuI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PpuMI PspOMI PsrI PvuII RsrII SacI
SacII SanDI SfiI SfoI SrfI SwaI