Restriction map for
pFN21AE1969
(Circular) (Six-base) MAPSORT of: pFN21AE1969.seq Check: 7720 from: 1 to: 6307
pFN21AE1969 6307 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2290 2290
Size: 6307
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4117 278
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3120 3650 1937
Size: 1183 530 4594
Fragments arranged by size:
4594 1183 530
Acc65I G'GTAC_C
Cuts at: 3535 3628 3535
Size: 93 6214
AclI AA'CG_TT
Cuts at: 1563 4434 4807 1563
Size: 2871 373 3063
Fragments arranged by size:
3063 2871 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2302 4370 5754 6022 1632
Size: 670 2068 1384 268 1917
Fragments arranged by size:
2068 1917 1384 670 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6110
AflIII A'CryG_T
Cuts at: 1203 5206 6183 1203
Size: 4003 977 1327
Fragments arranged by size:
4003 1327 977
AgeI A'CCGG_T
Cuts at: 5138 5138
Size: 6307
AhdI GACnn_n'nnGTC
Cuts at: 5036 5036
Size: 6307
AleI CACnn'nnGTG
Cuts at: 1166 2570 2600 1166
Size: 1404 30 4873
Fragments arranged by size:
4873 1404 30
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6307
AlwNI CAG_nnn'CTG
Cuts at: 3293 4050 5622 3293
Size: 757 1572 3978
Fragments arranged by size:
3978 1572 757
ApaLI G'TGCA_C
Cuts at: 4364 5520 4364
Size: 1156 5151
ApoI r'AATT_y
Cuts at: 1493 2660 3616 3842 3994 6103 1493
Size: 1167 956 226 152 2109 1697
Fragments arranged by size:
2109 1697 1167 956 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4861 160
Size: 872 3829 1606
Fragments arranged by size:
3829 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 3446 3632 1870
Size: 73 1221 282 186 4545
Fragments arranged by size:
4545 1221 282 186 73
AvrII C'CTAG_G
Cuts at: 3565 3565
Size: 6307
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3519 3552 3519
Size: 33 6274
BamHI G'GATC_C
Cuts at: 2626 3637 4004 2626
Size: 1011 367 4929
Fragments arranged by size:
4929 1011 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2417 2594 3314 3535 3628
Size: 325 217 627 630 177 720 221 93
Cuts at: 3628 5083 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 720 630 627 325 221 217
177 93
BanII G_rGCy'C
Cuts at: 1391 2217 3626 1391
Size: 826 1409 4072
Fragments arranged by size:
4072 1409 826
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5336
BbvCI CC'TCA_GC
Cuts at: 3209 3381 3209
Size: 172 6135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4498 4532 1903
Size: 34 2561 34 3678
Fragments arranged by size:
3678 2561 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3610 4199 5409 3610
Size: 589 1210 4508
Fragments arranged by size:
4508 1210 589
BclI T'GATC_A
Cuts at: 1548 2292 1548
Size: 744 5563
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4918 136
Size: 107 122 71 631 605 3246 1525
Fragments arranged by size:
3246 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6302 6302
Size: 6307
BlpI GC'TnA_GC
Cuts at: 3724 3724
Size: 6307
Bme1580I G_kGCm'C
Cuts at: 1790 2422 3319 4368 5524 1790
Size: 632 897 1049 1156 2573
Fragments arranged by size:
2573 1156 1049 897 632
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6307
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2937 4038 4996
Size: 416 53 83 399 24 1520 1101 958
Cuts at: 4996 442
Size: 1753
Fragments arranged by size:
1753 1520 1101 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2858 2858
Size: 6307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2144 4967 1239
Size: 905 2823 2579
Fragments arranged by size:
2823 2579 905
Bpu10I CC'TnA_GC
Cuts at: 3209 3252 3333 3381 3209
Size: 43 81 48 6135
Fragments arranged by size:
6135 81 48 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 3488 4432 5297 5595 2101
Size: 1387 944 865 298 2813
Fragments arranged by size:
2813 1387 944 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3347 3521 4970 882
Size: 617 1848 174 1449 2219
Fragments arranged by size:
2219 1848 1449 617 174
BsaAI yAC'GTr
Cuts at: 493 1204 2390 3411 3493 4098 493
Size: 711 1186 1021 82 605 2702
Fragments arranged by size:
2702 1186 1021 711 605 82
BsaBI GATnn'nnATC
Cuts at: 6301 6301
Size: 6307
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2342 4114 4496 5131
Size: 53 83 186 936 809 1772 382 635
Cuts at: 5131 275
Size: 1451
Fragments arranged by size:
1772 1451 936 809 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3507 4740 5138 5412 5559 5970 3507
Size: 1233 398 274 147 411 3844
Fragments arranged by size:
3844 1233 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2345 2375 2903 2933 1178
Size: 30 165 30 942 30 528 30 4552
Fragments arranged by size:
4552 942 528 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2911 1186
Size: 1725 4582
BseYI C'CCAG_C
Cuts at: 2963 3268 5510 6006 2963
Size: 305 2242 496 3264
Fragments arranged by size:
3264 2242 496 305
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3305 3482 1163
Size: 573 1569 177 3988
Fragments arranged by size:
3988 1569 573 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3757 4518 5546 1540
Size: 275 186 1756 761 1028 2301
Fragments arranged by size:
2301 1756 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2217 3339 4368 4453 5524 2217
Size: 1122 1029 85 1071 3000
Fragments arranged by size:
3000 1122 1071 1029 85
BsmI GAATG_Cn'
Cuts at: 2111 3818 3911 2111
Size: 1707 93 4507
Fragments arranged by size:
4507 1707 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2745 2904 3197 2745
Size: 159 293 5855
Fragments arranged by size:
5855 293 159
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2422 3319 3339 3626 4368 4453
Size: 399 427 205 897 20 287 742 85
Cuts at: 4453 5524 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 897 742 427 399 287 205
85 20
BspEI T'CCGG_A
Cuts at: 5970 5970
Size: 6307
BspHI T'CATG_A
Cuts at: 4196 5926 4196
Size: 1730 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2290 3423 3662 844
Size: 1446 1133 239 3489
Fragments arranged by size:
3489 1446 1133 239
BsrBI CCG'CTC
Cuts at: 1394 1823 4194 1394
Size: 429 2371 3507
Fragments arranged by size:
3507 2371 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4802 4976 65
Size: 1183 3554 174 1396
Fragments arranged by size:
3554 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3399 3428 4951 5138 1700
Size: 110 1589 29 1523 187 2869
Fragments arranged by size:
2869 1589 1523 187 110 29
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6307
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6307
BssSI C'ACGA_G
Cuts at: 4367 5379 4367
Size: 1012 5295
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6307
BstBI TT'CG_AA
Cuts at: 2658 5111 2658
Size: 2453 3854
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6307
BstXI CCAn_nnnn'nTGG
Cuts at: 2338 2572 2971 2338
Size: 234 399 5674
Fragments arranged by size:
5674 399 234
BstYI r'GATC_y
Cuts at: 1972 2626 3637 4004 4389 4406 5858 5944 5956
Size: 654 1011 367 385 17 1452 86 12
Cuts at: 5956 6302 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1011 654 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2622 2622
Size: 6307
BtgI C'CryG_G
Cuts at: 513 1065 2049 2880 3309 3434 513
Size: 552 984 831 429 125 3386
Fragments arranged by size:
3386 984 831 552 429 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3010 487
Size: 683 56 1784 3784
Fragments arranged by size:
3784 1784 683 56
BtsI GCAGTG_nn'
Cuts at: 2641 3482 3823 4617 4637 2641
Size: 841 341 794 20 4311
Fragments arranged by size:
4311 841 794 341 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6307
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6272
DraI TTT'AAA
Cuts at: 2505 3611 2505
Size: 1106 5201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5314 809
Size: 507 3998 1802
Fragments arranged by size:
3998 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2973 3078 3306
Size: 54 1425 50 275 18 1143 105 228
Cuts at: 3306 3341 3754 4643 6272 8
Size: 35 413 889 1629 43
Fragments arranged by size:
1629 1425 1143 889 413 275 228 105
54 50 43 35 18
EagI C'GGCC_G
Cuts at: 1537 1812 3754 1537
Size: 275 1942 4090
Fragments arranged by size:
4090 1942 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2400 2817 4237 1353
Size: 1047 417 1420 3423
Fragments arranged by size:
3423 1420 1047 417
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2174 2845 4894 5268 5414 387
Size: 1064 138 585 671 2049 374 146 1280
Fragments arranged by size:
2049 1280 1064 671 585 374 146 138
EcoICRI GAG'CTC
Cuts at: 2215 2215
Size: 6307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2144 2302 4370 4967 5754 6022 1239
Size: 393 512 158 2068 597 787 268 1524
Fragments arranged by size:
2068 1524 787 597 512 393 268 158
EcoO109I rG'GnC_Cy
Cuts at: 2617 2617
Size: 6307
EcoRI G'AATT_C
Cuts at: 2660 3616 3994 2660
Size: 956 378 4973
Fragments arranged by size:
4973 956 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 3122 2671
Size: 451 5856
FseI GG_CCGG'CC
Cuts at: 3432 3432
Size: 6307
FspI TGC'GCA
Cuts at: 4813 4813
Size: 6307
HaeII r_GCGC'y
Cuts at: 1678 3294 5454 1678
Size: 1616 2160 2531
Fragments arranged by size:
2531 2160 1616
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2345 2377 2903 2935 4948
Size: 32 135 32 773 32 526 32 2013
Cuts at: 4948 4980 5022 5054 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2013 773 526 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3480 3651 3897 669
Size: 988 281 1542 171 246 3079
Fragments arranged by size:
3079 1542 988 281 246 171
HindIII A'AGCT_T
Cuts at: 748 3104 748
Size: 2356 3951
HpaI GTT'AAC
Cuts at: 3897 3897
Size: 6307
KpnI G_GTAC'C
Cuts at: 3539 3632 3539
Size: 93 6214
MfeI C'AATT_G
Cuts at: 3906 3906
Size: 6307
MluI A'CGCG_T
Cuts at: 6183 6183
Size: 6307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2240 5421 5605 1305
Size: 935 3181 184 2007
Fragments arranged by size:
3181 2007 935 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2975 3080 3343 10
Size: 54 1425 343 1143 105 263 2974
Fragments arranged by size:
2974 1425 1143 343 263 105 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2570 2600 2885 4265 4624 4783 518
Size: 648 1404 30 285 1380 359 159 2042
Fragments arranged by size:
2042 1404 1380 648 359 285 159 30
MspA1I CmG'CkG
Cuts at: 1606 3541 3723 4400 5548 5793 1606
Size: 1935 182 677 1148 245 2120
Fragments arranged by size:
2120 1935 1148 677 245 182
NaeI GCC'GGC
Cuts at: 3430 3430
Size: 6307
NcoI C'CATG_G
Cuts at: 513 1065 2049 2880 513
Size: 552 984 831 3940
Fragments arranged by size:
3940 984 831 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6307
NgoMIV G'CCGG_C
Cuts at: 3428 3428
Size: 6307
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6307
NotI GC'GGCC_GC
Cuts at: 3754 3754
Size: 6307
NspI r_CATG'y
Cuts at: 1132 1229 3665 5210 1132
Size: 97 2436 1545 2229
Fragments arranged by size:
2436 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6307
PciI A'CATG_T
Cuts at: 5206 5206
Size: 6307
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3578 1108
Size: 2470 3837
PfoI T'CCnGG_A
Cuts at: 2853 2853
Size: 6307
PmeI GTTT'AAAC
Cuts at: 3611 3611
Size: 6307
PmlI CAC'GTG
Cuts at: 3493 3493
Size: 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 5889 5921 4376
Size: 32 1481 32 4762
Fragments arranged by size:
4762 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6307
PsiI TTA'TAA
Cuts at: 3877 3877
Size: 6307
PspXI vC'TCGA_Gb
Cuts at: 3164 3164
Size: 6307
PstI C_TGCA'G
Cuts at: 830 3161 3546 3659 830
Size: 2331 385 113 3478
Fragments arranged by size:
3478 2331 385 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6307
SacI G_AGCT'C
Cuts at: 2217 2217
Size: 6307
SalI G'TCGA_C
Cuts at: 1936 3649 1936
Size: 1713 4594
SbfI CC_TGCA'GG
Cuts at: 3659 3659
Size: 6307
ScaI AGT'ACT
Cuts at: 4555 6267 4555
Size: 1712 4595
SfcI C'TryA_G
Cuts at: 826 1045 3157 3542 3655 4790 5471 5662 826
Size: 219 2112 385 113 1135 681 191 1471
Fragments arranged by size:
2112 1471 1135 681 385 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6307
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6307
SmaI CCC'GGG
Cuts at: 1872 3634 1872
Size: 1762 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2116 3164 3467 4411 5312 5574
Size: 197 926 173 1048 303 944 901 262
Cuts at: 5574 820
Size: 1553
Fragments arranged by size:
1553 1048 944 926 901 303 262 197
173
SnaBI TAC'GTA
Cuts at: 493 4098 493
Size: 3605 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6307
SphI G_CATG'C
Cuts at: 1132 3665 1132
Size: 2533 3774
SspI AAT'ATT
Cuts at: 5 52 4231 5
Size: 47 4179 2081
Fragments arranged by size:
4179 2081 47
StuI AGG'CCT
Cuts at: 1517 2136 2980 3011 3174 1517
Size: 619 844 31 163 4650
Fragments arranged by size:
4650 844 619 163 31
StyI C'CwwG_G
Cuts at: 513 1065 2016 2049 2880 3565 3746 513
Size: 552 951 33 831 685 181 3074
Fragments arranged by size:
3074 951 831 685 552 181 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2919 3302 4334 4351 4504 4689 5134
Size: 1255 1339 383 1032 17 153 185 445
Cuts at: 5134 325
Size: 1498
Fragments arranged by size:
1498 1339 1255 1032 445 383 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4553 6265 96
Size: 274 80 33 51 1443 2576 1712 138
Fragments arranged by size:
2576 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6267 6299 6267
Size: 32 6275
Tth111I GACn'n_nGTC
Cuts at: 1316 2903 1316
Size: 1587 4720
XbaI T'CTAG_A
Cuts at: 3643 3643
Size: 6307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2056 2680 3073 2056
Size: 624 393 5290
Fragments arranged by size:
5290 624 393
XhoI C'TCGA_G
Cuts at: 1943 3164 1943
Size: 1221 5086
XmaI C'CCGG_G
Cuts at: 1870 3632 1870
Size: 1762 4545
XmnI GAAnn'nnTTC
Cuts at: 4436 4436
Size: 6307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4115 276
Size: 53 83 186 936 2581 2468
Fragments arranged by size:
2581 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BfrBI BsiWI BstZ17I
DraIII EcoNI EcoRV FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI