Restriction map for
pFN21AE1966
(Circular) (Six-base) MAPSORT of: pFN21AE1966.seq Check: 7295 from: 1 to: 6277
pFN21AE1966 6277 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3456 3456
Size: 6277
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3571 3620 1937
Size: 1634 49 4594
Fragments arranged by size:
4594 1634 49
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2811 3059 3198 3228 4340 5724 5992 1632
Size: 1179 248 139 30 1112 1384 268 1917
Fragments arranged by size:
1917 1384 1179 1112 268 248 139 30
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 3276 5176 6153 1203
Size: 2073 1900 977 1327
Fragments arranged by size:
2073 1900 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 3559 4020 5592 3559
Size: 461 1572 4244
Fragments arranged by size:
4244 1572 461
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2312 2493 2569 3388 3586 3812 3964 6073
Size: 819 181 76 819 198 226 152 2109
Cuts at: 6073 1493
Size: 1697
Fragments arranged by size:
2109 1697 819 819 226 198 181 152
76
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 3602 1870
Size: 73 1659 4545
Fragments arranged by size:
4545 1659 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3598 5053 618
Size: 325 217 627 1811 1455 1842
Fragments arranged by size:
1842 1811 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2414 2771 3596 1391
Size: 1023 357 825 4072
Fragments arranged by size:
4072 1023 825 357
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2461 2965 3065 928
Size: 971 562 504 100 4140
Fragments arranged by size:
4140 971 562 504 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2849 3307 4169 5379 2849
Size: 458 862 1210 3747
Fragments arranged by size:
3747 1210 862 458
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BfrBI ATG'CAT
Cuts at: 3455 3455
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2921 4888 136
Size: 107 122 71 631 605 1249 1967 1525
Fragments arranged by size:
1967 1525 1249 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 3422 4338 5494 1790
Size: 1632 916 1156 2573
Fragments arranged by size:
2573 1632 1156 916
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4008 4966 442
Size: 416 53 83 399 24 2591 958 1753
Fragments arranged by size:
2591 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4937 1239
Size: 3698 2579
Bpu10I CC'TnA_GC
Cuts at: 3396 3396
Size: 6277
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2239 4402 5267 5565 2057
Size: 182 2163 865 298 2769
Fragments arranged by size:
2769 2163 865 298 182
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4940 882
Size: 617 3441 2219
Fragments arranged by size:
3441 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2303 4710 5108 5382 5529 5940 2303
Size: 2407 398 274 147 411 2640
Fragments arranged by size:
2640 2407 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3375 1186
Size: 2189 4088
BseYI C'CCAG_C
Cuts at: 3198 5480 5976 3198
Size: 2282 496 3499
Fragments arranged by size:
3499 2282 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5704
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4338 4423 5494 4338
Size: 85 1071 5121
Fragments arranged by size:
5121 1071 85
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2414 2771 3422 3596 4338 4423 5494
Size: 399 624 357 651 174 742 85 1071
Cuts at: 5494 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 651 624 399 357 174
85
BspEI T'CCGG_A
Cuts at: 2303 5940 2303
Size: 3637 2640
BspHI T'CATG_A
Cuts at: 2137 4166 5896 2137
Size: 2029 1730 2518
Fragments arranged by size:
2518 2029 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3456 3632 844
Size: 2612 176 3489
Fragments arranged by size:
3489 2612 176
BsrBI CCG'CTC
Cuts at: 1394 1823 2253 4164 1394
Size: 429 430 1911 3507
Fragments arranged by size:
3507 1911 430 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2037 2721 3409 3455 4772 4946 65
Size: 1183 789 684 688 46 1317 174 1396
Fragments arranged by size:
1396 1317 1183 789 688 684 174 46
BsrFI r'CCGG_y
Cuts at: 1700 1810 4921 5108 1700
Size: 110 3111 187 2869
Fragments arranged by size:
3111 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3092 3167 3440 794
Size: 2298 75 273 3631
Fragments arranged by size:
3631 2298 273 75
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 2808 3607 3974 4359 4376 5828 5914 5926
Size: 836 799 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 836 799 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2445 2445
Size: 6277
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4786
Fragments arranged by size:
4786 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 2080 3793 4587 4607 2080
Size: 1713 794 20 3750
Fragments arranged by size:
3750 1713 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6242
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 2647 3724
Size: 54 1425 50 275 18 253 564 1077
Cuts at: 3724 4613 6242 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1077 889 564 275 253 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 3513 4207 1353
Size: 1155 1005 694 3423
Fragments arranged by size:
3423 1155 1005 694
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3108 4864 5238 5384 387
Size: 1064 138 1519 1756 374 146 1280
Fragments arranged by size:
1756 1519 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2811 3059 3198 3228 4340 4937 5724
Size: 393 1179 248 139 30 1112 597 787
Cuts at: 5724 5992 1239
Size: 268 1524
Fragments arranged by size:
1524 1179 1112 787 597 393 268 248
139 30
EcoRI G'AATT_C
Cuts at: 2493 3586 3964 2493
Size: 1093 378 4806
Fragments arranged by size:
4806 1093 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2051 2816 2051
Size: 765 5512
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 5424 1678
Size: 3746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2113 2145 2603 2635 4918
Size: 32 135 32 541 32 458 32 2283
Cuts at: 4918 4950 4992 5024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2283 541 458 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 3621 3867 669
Size: 988 281 1444 239 246 3079
Fragments arranged by size:
3079 1444 988 281 246 239
HindIII A'AGCT_T
Cuts at: 748 3210 748
Size: 2462 3815
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3426 3876 3426
Size: 450 5827
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3286 3289 3536 5391 5575 1305
Size: 1981 3 247 1855 184 2007
Fragments arranged by size:
2007 1981 1855 247 184 3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2649 10
Size: 54 1425 343 817 3638
Fragments arranged by size:
3638 1425 817 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3375 4235 4594 4753 518
Size: 648 2209 860 359 159 2042
Fragments arranged by size:
2209 2042 860 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2482 2845 3230 3693 4370 5518 5763 1606
Size: 876 363 385 463 677 1148 245 2120
Fragments arranged by size:
2120 1148 876 677 463 385 363 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4786
Fragments arranged by size:
4786 939 552
NdeI CA'TA_TG
Cuts at: 387 3499 387
Size: 3112 3165
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NsiI A_TGCA'T
Cuts at: 3457 3457
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 3280 3635 5180 1132
Size: 97 2051 355 1545 2229
Fragments arranged by size:
2229 2051 1545 355 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 3276 5176 3276
Size: 1900 4377
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6277
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2995 3027 4346 4378 5859 5891 2995
Size: 32 1319 32 1481 32 3381
Fragments arranged by size:
3381 1481 1319 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2523 2555 2523
Size: 32 6245
PstI C_TGCA'G
Cuts at: 830 2713 3229 3436 3629 830
Size: 1883 516 207 193 3478
Fragments arranged by size:
3478 1883 516 207 193
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2482 2845 3230 2482
Size: 363 385 5529
Fragments arranged by size:
5529 385 363
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2287 2526 2709 3140 3225 3432 3625
Size: 219 1242 239 183 431 85 207 193
Cuts at: 3625 4760 5441 5632 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1242 1135 681 431 239 219 207
193 191 183 85
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 3604 1872
Size: 1732 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2036 2254 4381 5282 5544 820
Size: 197 926 93 218 2127 901 262 1553
Fragments arranged by size:
2127 1553 926 901 262 218 197 93
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6277
StyI C'CwwG_G
Cuts at: 513 1065 2004 2501 2874 3716 513
Size: 552 939 497 373 842 3074
Fragments arranged by size:
3074 939 842 552 497 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4304 4321 4474 4659 5104 325
Size: 1255 2724 17 153 185 445 1498
Fragments arranged by size:
2724 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2677 4523 6235
Size: 274 80 33 51 1443 700 1846 1712
Cuts at: 6235 96
Size: 138
Fragments arranged by size:
1846 1712 1443 700 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3246 3278 6237 6269 3246
Size: 32 2959 32 3254
Fragments arranged by size:
3254 2959 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2923 2923
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 3602 1870
Size: 1732 4545
XmnI GAAnn'nnTTC
Cuts at: 4406 4406
Size: 6277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI