Restriction map for
pFN21AE1961
(Circular) (Six-base) MAPSORT of: pFN21AE1961.seq Check: 6485 from: 1 to: 6277
pFN21AE1961 6277 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2752 2752
Size: 6277
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2937 3620 1937
Size: 1000 683 4594
Fragments arranged by size:
4594 1000 683
Acc65I G'GTAC_C
Cuts at: 3333 3598 3333
Size: 265 6012
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3451 3534 4340 5724 5992 1632
Size: 1819 83 806 1384 268 1917
Fragments arranged by size:
1917 1819 1384 806 268 83
AflII C'TTAA_G
Cuts at: 820 1017 3452 820
Size: 197 2435 3645
Fragments arranged by size:
3645 2435 197
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 5108 5108
Size: 6277
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6277
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6277
AlwNI CAG_nnn'CTG
Cuts at: 3283 4020 5592 3283
Size: 737 1572 3968
Fragments arranged by size:
3968 1572 737
ApaI G_GGCC'C
Cuts at: 3350 3350
Size: 6277
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 2900 3492 3542 3586 3812 3964 6073 1493
Size: 1407 592 50 44 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 592 226 152 50 44
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 2637 2979 3602 1870
Size: 73 694 342 623 4545
Fragments arranged by size:
4545 694 623 342 73
AvrII C'CTAG_G
Cuts at: 3173 3173
Size: 6277
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2800 2833 3325 3358 2800
Size: 33 492 33 5719
Fragments arranged by size:
5719 492 33 33
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2246 3333 3598 5053 618
Size: 325 217 627 459 1087 265 1455 1842
Fragments arranged by size:
1842 1455 1087 627 459 325 265 217
BanII G_rGCy'C
Cuts at: 1391 2169 2460 2733 3217 3350 3596 1391
Size: 778 291 273 484 133 246 4072
Fragments arranged by size:
4072 778 484 291 273 246 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5306
BbvCI CC'TCA_GC
Cuts at: 3135 3135
Size: 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4468 4502 1903
Size: 34 2531 34 3678
Fragments arranged by size:
3678 2531 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2002 2828 4169 5379 2002
Size: 826 1341 1210 2900
Fragments arranged by size:
2900 1341 1210 826
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6277
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3188 4888 136
Size: 107 122 71 631 605 1516 1700 1525
Fragments arranged by size:
1700 1525 1516 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 2512 2566 3350 4338 5494 1790
Size: 722 54 784 988 1156 2573
Fragments arranged by size:
2573 1156 988 784 722 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2227 2949 4008
Size: 416 53 83 399 24 810 722 1059
Cuts at: 4008 4966 442
Size: 958 1753
Fragments arranged by size:
1753 1059 958 810 722 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2181 2450 2747 3113 3203 4937 1239
Size: 942 269 297 366 90 1734 2579
Fragments arranged by size:
2579 1734 942 366 297 269 90
Bpu10I CC'TnA_GC
Cuts at: 2732 3135 2732
Size: 403 5874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3001 4402 5267 5565 3001
Size: 1401 865 298 3713
Fragments arranged by size:
3713 1401 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2219 4940 882
Size: 617 720 2721 2219
Fragments arranged by size:
2721 2219 720 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4084 4466 5101 275
Size: 53 83 186 936 2551 382 635 1451
Fragments arranged by size:
2551 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2643 4710 5108 5382 5529 5940 2643
Size: 2067 398 274 147 411 2980
Fragments arranged by size:
2980 2067 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6052
Fragments arranged by size:
6052 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2474 2684 3152 1186
Size: 1288 210 468 4311
Fragments arranged by size:
4311 1288 468 210
BseYI C'CCAG_C
Cuts at: 5480 5976 5480
Size: 496 5781
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5704
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3727 4488 5516 1540
Size: 275 186 1726 761 1028 2301
Fragments arranged by size:
2301 1726 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2733 4338 4423 5494 2259
Size: 474 1605 85 1071 3042
Fragments arranged by size:
3042 1605 1071 474 85
BsmI GAATG_Cn'
Cuts at: 2474 3788 3881 2474
Size: 1314 93 4870
Fragments arranged by size:
4870 1314 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3131 3131
Size: 6277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2259 2460 2512 2566 2733 3217
Size: 399 379 90 201 52 54 167 484
Cuts at: 3217 3350 3596 4338 4423 5494 1391
Size: 133 246 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 379 246 201
167 133 90 85 54 52
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2752 3237 3632 844
Size: 1908 485 395 3489
Fragments arranged by size:
3489 1908 485 395
BsrBI CCG'CTC
Cuts at: 1394 1823 2772 4164 1394
Size: 429 949 1392 3507
Fragments arranged by size:
3507 1392 949 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2277 4772 4946 65
Size: 1183 1029 2495 174 1396
Fragments arranged by size:
2495 1396 1183 1029 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4921 5108 1700
Size: 110 3111 187 2869
Fragments arranged by size:
3111 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6277
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6277
BstBI TT'CG_AA
Cuts at: 2054 5081 2054
Size: 3027 3250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6277
BstXI CCAn_nnnn'nTGG
Cuts at: 2920 2920
Size: 6277
BstYI r'GATC_y
Cuts at: 1972 3607 3974 4359 4376 5828 5914 5926 6272
Size: 1635 367 385 17 1452 86 12 346
Cuts at: 6272 1972
Size: 1977
Fragments arranged by size:
1977 1635 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2232 2469 2543 3204 513
Size: 552 939 228 237 74 661 3586
Fragments arranged by size:
3586 939 661 552 237 228 74
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5538
Fragments arranged by size:
5538 683 56
BtsI GCAGTG_nn'
Cuts at: 3102 3793 4587 4607 3102
Size: 691 794 20 4772
Fragments arranged by size:
4772 794 691 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6277
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3069 3104 529
Size: 35 2505 35 3702
Fragments arranged by size:
3702 2505 35 35
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5284 809
Size: 507 3968 1802
Fragments arranged by size:
3968 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2235 2712 2922
Size: 54 1425 50 275 18 405 477 210
Cuts at: 2922 2971 3207 3724 4613 6242 8
Size: 49 236 517 889 1629 43
Fragments arranged by size:
1629 1425 889 517 477 405 275 236
210 54 50 49 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2702 3036 3096 3510 4207 1353
Size: 1349 334 60 414 697 3423
Fragments arranged by size:
3423 1349 697 414 334 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2731 2731
Size: 6277
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2181 2450 2747 3113 3203 3451 3534
Size: 393 549 269 297 366 90 248 83
Cuts at: 3534 4340 4937 5724 5992 1239
Size: 806 597 787 268 1524
Fragments arranged by size:
1524 806 787 597 549 393 366 297
269 268 248 90 83
EcoO109I rG'GnC_Cy
Cuts at: 2502 3347 2502
Size: 845 5432
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FspI TGC'GCA
Cuts at: 3233 4783 3233
Size: 1550 4727
HaeII r_GCGC'y
Cuts at: 1678 5424 1678
Size: 3746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2771 2803 3518
Size: 32 135 32 428 32 739 32 715
Cuts at: 3518 3550 4918 4950 4992 5024 1373
Size: 32 1368 32 42 32 2626
Fragments arranged by size:
2626 1368 739 715 428 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3621 3867 669
Size: 988 281 1683 246 3079
Fragments arranged by size:
3079 1683 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6277
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3337 3602 3337
Size: 265 6012
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5391 5575 1305
Size: 4086 184 2007
Fragments arranged by size:
4086 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 2973 3209 10
Size: 54 1425 343 882 259 236 3078
Fragments arranged by size:
3078 1425 882 343 259 236 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4235 4594 4753 518
Size: 648 3069 359 159 2042
Fragments arranged by size:
3069 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2740 2999 3375 3693 4370 5518 5763 1606
Size: 1134 259 376 318 677 1148 245 2120
Fragments arranged by size:
2120 1148 1134 677 376 318 259 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2469 3204 513
Size: 552 939 465 735 3586
Fragments arranged by size:
3586 939 735 552 465
NdeI CA'TA_TG
Cuts at: 387 2919 387
Size: 2532 3745
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 2542 3635 5180 1132
Size: 97 1313 1093 1545 2229
Fragments arranged by size:
2229 1545 1313 1093 97
PasI CC'CwG_GG
Cuts at: 1348 3155 1348
Size: 1807 4470
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2710 3262 1108
Size: 1602 552 4123
Fragments arranged by size:
4123 1602 552
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3321 3353 4346 4378 5859 5891 3321
Size: 32 993 32 1481 32 3707
Fragments arranged by size:
3707 1481 993 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2502 2502
Size: 6277
PshAI GACnn'nnGTC
Cuts at: 1613 2098 1613
Size: 485 5792
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PspOMI G'GGCC_C
Cuts at: 3346 3346
Size: 6277
PstI C_TGCA'G
Cuts at: 830 2745 2763 2998 3279 3629 830
Size: 1915 18 235 281 350 3478
Fragments arranged by size:
3478 1915 350 281 235 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2740 2999 2740
Size: 259 6018
SacI G_AGCT'C
Cuts at: 2733 2733
Size: 6277
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SfcI C'TryA_G
Cuts at: 826 1045 2741 2759 2797 2994 3275 3625 4760
Size: 219 1696 18 38 197 281 350 1135
Cuts at: 4760 5441 5632 826
Size: 681 191 1471
Fragments arranged by size:
1696 1471 1135 681 350 281 219 197
191 38 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 3188 3188
Size: 6277
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 2981 3604 1872
Size: 1109 623 4545
Fragments arranged by size:
4545 1109 623
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3016 3452 4381 5282 5544 820
Size: 197 926 1073 436 929 901 262 1553
Fragments arranged by size:
1553 1073 929 926 901 436 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 3107 3320 1517
Size: 1590 213 4474
Fragments arranged by size:
4474 1590 213
StyI C'CwwG_G
Cuts at: 513 1065 2004 2469 3173 3204 3315 3716 513
Size: 552 939 465 704 31 111 401 3074
Fragments arranged by size:
3074 939 704 552 465 401 111 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4304 4321 4474 4659 5104 325
Size: 1255 2724 17 153 185 445 1498
Fragments arranged by size:
2724 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4523 6235 96
Size: 274 80 33 51 1443 2546 1712 138
Fragments arranged by size:
2546 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2249 2281 2502 2534 6237 6269 2249
Size: 32 221 32 3703 32 2257
Fragments arranged by size:
3703 2257 221 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6277
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2722
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 2979 3602 1870
Size: 1109 623 4545
Fragments arranged by size:
4545 1109 623
XmnI GAAnn'nnTTC
Cuts at: 2182 4406 2182
Size: 2224 4053
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BplI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfoI
SrfI SwaI