Restriction map for
pFN21AE1959
(Circular) (Six-base) MAPSORT of: pFN21AE1959.seq Check: 2650 from: 1 to: 6253
pFN21AE1959 6253 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2229 4063 278
Size: 53 83 186 936 693 1834 2468
Fragments arranged by size:
2468 1834 936 693 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3471 3596 1937
Size: 1534 125 4594
Fragments arranged by size:
4594 1534 125
Acc65I G'GTAC_C
Cuts at: 3574 3574
Size: 6253
AclI AA'CG_TT
Cuts at: 1563 3552 4380 4753 1563
Size: 1989 828 373 3063
Fragments arranged by size:
3063 1989 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2362 4316 5700 5968 1632
Size: 730 1954 1384 268 1917
Fragments arranged by size:
1954 1917 1384 730 268
AfeI AGC'GCT
Cuts at: 2216 2216
Size: 6253
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6056
AflIII A'CryG_T
Cuts at: 1203 3520 5152 6129 1203
Size: 2317 1632 977 1327
Fragments arranged by size:
2317 1632 1327 977
AgeI A'CCGG_T
Cuts at: 2395 5084 2395
Size: 2689 3564
AhdI GACnn_n'nnGTC
Cuts at: 4982 4982
Size: 6253
AleI CACnn'nnGTG
Cuts at: 1166 2255 1166
Size: 1089 5164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6253
AlwNI CAG_nnn'CTG
Cuts at: 2296 2390 2539 2552 3439 3996 5568 2296
Size: 94 149 13 887 557 1572 2981
Fragments arranged by size:
2981 1572 887 557 149 94 13
ApaLI G'TGCA_C
Cuts at: 4310 5466 4310
Size: 1156 5097
ApoI r'AATT_y
Cuts at: 1493 3562 3788 3940 6049 1493
Size: 2069 226 152 2109 1697
Fragments arranged by size:
2109 2069 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4807 160
Size: 872 3775 1606
Fragments arranged by size:
3775 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
AvaI C'yCGr_G
Cuts at: 1870 1943 2103 2156 3578 1870
Size: 73 160 53 1422 4545
Fragments arranged by size:
4545 1422 160 73 53
BamHI G'GATC_C
Cuts at: 3583 3950 3583
Size: 367 5886
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2415 2606 2858 3574 5029
Size: 325 217 627 628 191 252 716 1455
Cuts at: 5029 618
Size: 1842
Fragments arranged by size:
1842 1455 716 628 627 325 252 217
191
BanII G_rGCy'C
Cuts at: 1391 3572 1391
Size: 2181 4072
BbeI G_GCGC'C
Cuts at: 2419 2419
Size: 6253
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3217 928
Size: 971 1318 3964
Fragments arranged by size:
3964 1318 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3299 3333 4444 4478 1903
Size: 34 1362 34 1111 34 3678
Fragments arranged by size:
3678 1362 1111 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4145 5355 4145
Size: 1210 5043
BclI T'GATC_A
Cuts at: 1548 2520 1548
Size: 972 5281
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4864 136
Size: 107 122 71 631 605 3192 1525
Fragments arranged by size:
3192 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3405 6248 3405
Size: 2843 3410
BlpI GC'TnA_GC
Cuts at: 2267 2654 3670 2267
Size: 387 1016 4850
Fragments arranged by size:
4850 1016 387
Bme1580I G_kGCm'C
Cuts at: 1790 2559 2863 4314 5470 1790
Size: 769 304 1451 1156 2573
Fragments arranged by size:
2573 1451 1156 769 304
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6253
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2011 3984 4942
Size: 416 53 83 399 24 594 1973 958
Cuts at: 4942 442
Size: 1753
Fragments arranged by size:
1973 1753 958 594 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 3292 2710
Size: 582 5671
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2507 3007 3143 3461 4913 1239
Size: 1268 500 136 318 1452 2579
Fragments arranged by size:
2579 1452 1268 500 318 136
Bpu10I CC'TnA_GC
Cuts at: 2511 3291 2511
Size: 780 5473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2072 3355 4378 5243 5541 2072
Size: 1283 1023 865 298 2784
Fragments arranged by size:
2784 1283 1023 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3119 4916 882
Size: 617 1620 1797 2219
Fragments arranged by size:
2219 1797 1620 617
BsaAI yAC'GTr
Cuts at: 493 1204 4044 493
Size: 711 2840 2702
Fragments arranged by size:
2840 2702 711
BsaBI GATnn'nnATC
Cuts at: 6247 6247
Size: 6253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2226 2416 4060 4442
Size: 53 83 186 936 693 190 1644 382
Cuts at: 4442 5077 275
Size: 635 1451
Fragments arranged by size:
1644 1451 936 693 635 382 190 186
83 53
BsaWI w'CCGG_w
Cuts at: 2395 4686 5084 5358 5505 5916 2395
Size: 2291 398 274 147 411 2732
Fragments arranged by size:
2732 2291 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 1178
Size: 30 165 30 1026 30 4972
Fragments arranged by size:
4972 1026 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 2686 3130 1186
Size: 1318 182 444 4309
Fragments arranged by size:
4309 1318 444 182
BseYI C'CCAG_C
Cuts at: 2461 3093 5456 5952 2461
Size: 632 2363 496 2762
Fragments arranged by size:
2762 2363 632 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5680
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3703 4464 5492 1540
Size: 275 186 1702 761 1028 2301
Fragments arranged by size:
2301 1702 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2088 2817 4314 4399 5470 2088
Size: 729 1497 85 1071 2871
Fragments arranged by size:
2871 1497 1071 729 85
BsmI GAATG_Cn'
Cuts at: 2434 3764 3857 2434
Size: 1330 93 4830
Fragments arranged by size:
4830 1330 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2088 2559 2817 2863 3572 4314 4399
Size: 399 298 471 258 46 709 742 85
Cuts at: 4399 5470 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 709 471 399 298 258
85 46
BspEI T'CCGG_A
Cuts at: 5916 5916
Size: 6253
BspHI T'CATG_A
Cuts at: 2674 4142 5872 2674
Size: 1468 1730 3055
Fragments arranged by size:
3055 1730 1468
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 2745 3232 3608 844
Size: 1673 228 487 376 3489
Fragments arranged by size:
3489 1673 487 376 228
BsrBI CCG'CTC
Cuts at: 1394 1823 3067 3295 4140 1394
Size: 429 1244 228 845 3507
Fragments arranged by size:
3507 1244 845 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 4748 4922 65
Size: 1183 3500 174 1396
Fragments arranged by size:
3500 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2325 2395 2412 3372 4897 5084 1700
Size: 110 515 70 17 960 1525 187 2869
Fragments arranged by size:
2869 1525 960 515 187 110 70 17
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6253
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6253
BssSI C'ACGA_G
Cuts at: 2660 4313 5325 2660
Size: 1653 1012 3588
Fragments arranged by size:
3588 1653 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6253
BstBI TT'CG_AA
Cuts at: 5057 5057
Size: 6253
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6253
BstXI CCAn_nnnn'nTGG
Cuts at: 2139 2145 3264 2139
Size: 6 1119 5128
Fragments arranged by size:
5128 1119 6
BstYI r'GATC_y
Cuts at: 1972 2235 3030 3405 3583 3950 4335 4352 5804
Size: 263 795 375 178 367 385 17 1452
Cuts at: 5804 5890 5902 6248 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 795 385 375 367 346 263
178 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2138 513
Size: 552 939 134 4628
Fragments arranged by size:
4628 939 552 134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2434 2658 487
Size: 683 56 1208 224 4082
Fragments arranged by size:
4082 1208 683 224 56
BtsI GCAGTG_nn'
Cuts at: 3315 3769 4563 4583 3315
Size: 454 794 20 4985
Fragments arranged by size:
4985 794 454 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6253
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6218
DraI TTT'AAA
Cuts at: 3557 3557
Size: 6253
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5260 809
Size: 507 3944 1802
Fragments arranged by size:
3944 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2362 2592
Size: 54 1425 50 275 18 204 328 230
Cuts at: 2592 3393 3420 3700 4589 6218 8
Size: 801 27 280 889 1629 43
Fragments arranged by size:
1629 1425 889 801 328 280 275 230
204 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3700 1537
Size: 275 1888 4090
Fragments arranged by size:
4090 1888 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3419 4183 1353
Size: 2066 764 3423
Fragments arranged by size:
3423 2066 764
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4840 5214 5360 387
Size: 1064 138 3251 374 146 1280
Fragments arranged by size:
3251 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2507 3007 3143 3461 4316 4913
Size: 393 730 145 500 136 318 855 597
Cuts at: 4913 5700 5968 1239
Size: 787 268 1524
Fragments arranged by size:
1524 855 787 730 597 500 393 318
268 145 136
EcoO109I rG'GnC_Cy
Cuts at: 2107 2293 2515 2549 2885 3271 2107
Size: 186 222 34 336 386 5089
Fragments arranged by size:
5089 386 336 222 186 34
EcoRI G'AATT_C
Cuts at: 3562 3940 3562
Size: 378 5875
FseI GG_CCGG'CC
Cuts at: 2329 2329
Size: 6253
FspI TGC'GCA
Cuts at: 4759 4759
Size: 6253
HaeII r_GCGC'y
Cuts at: 1678 2218 2419 3373 5400 1678
Size: 540 201 954 2027 2531
Fragments arranged by size:
2531 2027 954 540 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4894 4926 4968 5000 1373
Size: 32 135 32 3322 32 42 32 2626
Fragments arranged by size:
3322 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2929 3247 3472 3597 3843 669
Size: 988 281 991 318 225 125 246 3079
Fragments arranged by size:
3079 991 988 318 281 246 225 125
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6253
HpaI GTT'AAC
Cuts at: 3843 3843
Size: 6253
KasI G'GCGC_C
Cuts at: 2415 2415
Size: 6253
KpnI G_GTAC'C
Cuts at: 3578 3578
Size: 6253
MfeI C'AATT_G
Cuts at: 3852 3852
Size: 6253
MluI A'CGCG_T
Cuts at: 6129 6129
Size: 6253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3157 5367 5551 1305
Size: 1852 2210 184 2007
Fragments arranged by size:
2210 2007 1852 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2364 2594 3395 3422 10
Size: 54 1425 343 532 230 801 27 2841
Fragments arranged by size:
2841 1425 801 532 343 230 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2255 4211 4570 4729 518
Size: 648 1089 1956 359 159 2042
Fragments arranged by size:
2042 1956 1089 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2898 3097 3513 3669 4346 5494 5739 1606
Size: 1292 199 416 156 677 1148 245 2120
Fragments arranged by size:
2120 1292 1148 677 416 245 199 156
NaeI GCC'GGC
Cuts at: 2327 2414 2327
Size: 87 6166
NarI GG'CG_CC
Cuts at: 2416 2416
Size: 6253
NcoI C'CATG_G
Cuts at: 513 1065 2004 2138 513
Size: 552 939 134 4628
Fragments arranged by size:
4628 939 552 134
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6253
NgoMIV G'CCGG_C
Cuts at: 2325 2412 2325
Size: 87 6166
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6253
NotI GC'GGCC_GC
Cuts at: 3700 3700
Size: 6253
NspI r_CATG'y
Cuts at: 1132 1229 2165 2498 3524 3611 5156 1132
Size: 97 936 333 1026 87 1545 2229
Fragments arranged by size:
2229 1545 1026 936 333 97 87
PasI CC'CwG_GG
Cuts at: 1348 2173 1348
Size: 825 5428
PciI A'CATG_T
Cuts at: 3520 5152 3520
Size: 1632 4621
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2985 1108
Size: 1877 4376
PfoI T'CCnGG_A
Cuts at: 2699 2808 2844 2984 3120 2699
Size: 109 36 140 136 5832
Fragments arranged by size:
5832 140 136 109 36
PmeI GTTT'AAAC
Cuts at: 3557 3557
Size: 6253
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4322 4354 5835 5867 4322
Size: 32 1481 32 4708
Fragments arranged by size:
4708 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 3271 2107
Size: 1164 5089
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6253
PsiI TTA'TAA
Cuts at: 3823 3823
Size: 6253
PstI C_TGCA'G
Cuts at: 830 2235 2757 2799 3605 830
Size: 1405 522 42 806 3478
Fragments arranged by size:
3478 1405 806 522 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6253
PvuII CAG'CTG
Cuts at: 3097 3097
Size: 6253
SalI G'TCGA_C
Cuts at: 1936 3470 3595 1936
Size: 1534 125 4594
Fragments arranged by size:
4594 1534 125
SanDI GG'GwC_CC
Cuts at: 2107 2107
Size: 6253
SbfI CC_TGCA'GG
Cuts at: 3605 3605
Size: 6253
ScaI AGT'ACT
Cuts at: 4501 6213 4501
Size: 1712 4541
SfcI C'TryA_G
Cuts at: 826 1045 2231 2753 2795 3431 3601 4736 5417
Size: 219 1186 522 42 636 170 1135 681
Cuts at: 5417 5608 826
Size: 191 1471
Fragments arranged by size:
1471 1186 1135 681 636 522 219 191
170 42
SfoI GGC'GCC
Cuts at: 2417 2417
Size: 6253
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6253
SgrAI Cr'CCGG_yG
Cuts at: 1700 2412 3372 1700
Size: 712 960 4581
Fragments arranged by size:
4581 960 712
SmaI CCC'GGG
Cuts at: 1872 3580 1872
Size: 1708 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2087 3334 4357 5258 5520 820
Size: 197 926 144 1247 1023 901 262 1553
Fragments arranged by size:
1553 1247 1023 926 901 262 197 144
SnaBI TAC'GTA
Cuts at: 493 4044 493
Size: 3551 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6253
SphI G_CATG'C
Cuts at: 1132 3611 1132
Size: 2479 3774
SspI AAT'ATT
Cuts at: 5 52 4177 5
Size: 47 4125 2081
Fragments arranged by size:
4125 2081 47
StuI AGG'CCT
Cuts at: 1517 2101 2118 2768 3113 1517
Size: 584 17 650 345 4657
Fragments arranged by size:
4657 650 584 345 17
StyI C'CwwG_G
Cuts at: 513 1065 2004 2138 3692 513
Size: 552 939 134 1554 3074
Fragments arranged by size:
3074 1554 939 552 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2071 4280 4297 4450 4635 5080 325
Size: 1255 491 2209 17 153 185 445 1498
Fragments arranged by size:
2209 1498 1255 491 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4499 6211 96
Size: 274 80 33 51 1443 2522 1712 138
Fragments arranged by size:
2522 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6213 6245 6213
Size: 32 6221
Tth111I GACn'n_nGTC
Cuts at: 1316 2966 1316
Size: 1650 4603
XbaI T'CTAG_A
Cuts at: 3589 3589
Size: 6253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2068 2136 2257 2068
Size: 68 121 6064
Fragments arranged by size:
6064 121 68
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6253
XmaI C'CCGG_G
Cuts at: 1870 3578 1870
Size: 1708 4545
XmnI GAAnn'nnTTC
Cuts at: 3197 4382 3197
Size: 1185 5068
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2227 4061 276
Size: 53 83 186 936 693 1834 2468
Fragments arranged by size:
2468 1834 936 693 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FspAI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI