Restriction map for
pFN21AE1958
(Circular) (Six-base) MAPSORT of: pFN21AE1958.seq Check: 3199 from: 1 to: 6247
pFN21AE1958 6247 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3220 3220
Size: 6247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4057 278
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2416 3590 1937
Size: 479 1174 4594
Fragments arranged by size:
4594 1174 479
Acc65I G'GTAC_C
Cuts at: 3568 3568
Size: 6247
AclI AA'CG_TT
Cuts at: 1563 4374 4747 1563
Size: 2811 373 3063
Fragments arranged by size:
3063 2811 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2612 2772 4310 5694 5962 1632
Size: 980 160 1538 1384 268 1917
Fragments arranged by size:
1917 1538 1384 980 268 160
AfeI AGC'GCT
Cuts at: 2815 2815
Size: 6247
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6050
AflIII A'CryG_T
Cuts at: 1203 2214 3134 5146 6123 1203
Size: 1011 920 2012 977 1327
Fragments arranged by size:
2012 1327 1011 977 920
AgeI A'CCGG_T
Cuts at: 5078 5078
Size: 6247
AhdI GACnn_n'nnGTC
Cuts at: 4976 4976
Size: 6247
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6247
AlwNI CAG_nnn'CTG
Cuts at: 2029 2137 2162 2538 2875 3990 5562 2029
Size: 108 25 376 337 1115 1572 2714
Fragments arranged by size:
2714 1572 1115 376 337 108 25
ApaI G_GGCC'C
Cuts at: 2036 2587 2826 2036
Size: 551 239 5457
Fragments arranged by size:
5457 551 239
ApaLI G'TGCA_C
Cuts at: 4304 5460 4304
Size: 1156 5091
ApoI r'AATT_y
Cuts at: 1493 3556 3782 3934 6043 1493
Size: 2063 226 152 2109 1697
Fragments arranged by size:
2109 2063 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4801 160
Size: 872 3769 1606
Fragments arranged by size:
3769 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
AvaI C'yCGr_G
Cuts at: 1870 1943 2773 2951 3054 3572 1870
Size: 73 830 178 103 518 4545
Fragments arranged by size:
4545 830 518 178 103 73
AvrII C'CTAG_G
Cuts at: 3469 3469
Size: 6247
BamHI G'GATC_C
Cuts at: 3380 3577 3944 3380
Size: 197 367 5683
Fragments arranged by size:
5683 367 197
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2088 2372 3433 3474 3568
Size: 325 217 627 301 284 1061 41 94
Cuts at: 3568 5023 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1061 627 325 301 284 217
94 41
BanII G_rGCy'C
Cuts at: 1391 2036 2126 2587 2826 3243 3566 1391
Size: 645 90 461 239 417 323 4072
Fragments arranged by size:
4072 645 461 417 323 239 90
BbeI G_GCGC'C
Cuts at: 2092 2376 3478 2092
Size: 284 1102 4861
Fragments arranged by size:
4861 1102 284
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5276
BbvCI CC'TCA_GC
Cuts at: 3077 3077
Size: 6247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2686 2720 2919 2953 4438 4472 1903
Size: 34 749 34 199 34 1485 34 3678
Fragments arranged by size:
3678 1485 749 199 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2689 4139 5349 2689
Size: 1450 1210 3587
Fragments arranged by size:
3587 1450 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6247
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2498 2582 4858
Size: 107 122 71 631 605 826 84 2276
Cuts at: 4858 136
Size: 1525
Fragments arranged by size:
2276 1525 826 631 605 122 107 84
71
BglII A'GATC_T
Cuts at: 6242 6242
Size: 6247
BlpI GC'TnA_GC
Cuts at: 2810 2921 3664 2810
Size: 111 743 5393
Fragments arranged by size:
5393 743 111
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2587 2826 4308 5464 1790
Size: 246 551 239 1482 1156 2573
Fragments arranged by size:
2573 1482 1156 551 246 239
BmgBI CAC'GTC
Cuts at: 1318 2674 1318
Size: 1356 4891
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2017 2257 3978
Size: 416 53 83 399 24 600 240 1721
Cuts at: 3978 4936 442
Size: 958 1753
Fragments arranged by size:
1753 1721 958 600 416 399 240 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6247
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3463 3463
Size: 6247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2055 2210 2445 2515 2738 4907 1239
Size: 816 155 235 70 223 2169 2579
Fragments arranged by size:
2579 2169 816 235 223 155 70
Bpu10I CC'TnA_GC
Cuts at: 2144 3077 2144
Size: 933 5314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2772 3494 4372 5237 5535 2772
Size: 722 878 865 298 3484
Fragments arranged by size:
3484 878 865 722 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2491 4910 882
Size: 617 992 2419 2219
Fragments arranged by size:
2419 2219 992 617
BsaAI yAC'GTr
Cuts at: 493 1204 4038 493
Size: 711 2834 2702
Fragments arranged by size:
2834 2702 711
BsaBI GATnn'nnATC
Cuts at: 6241 6241
Size: 6247
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2089 2373 3475 4054
Size: 53 83 186 936 556 284 1102 579
Cuts at: 4054 4436 5071 275
Size: 382 635 1451
Fragments arranged by size:
1451 1102 936 635 579 556 382 284
186 83 53
BsaWI w'CCGG_w
Cuts at: 4680 5078 5352 5499 5910 4680
Size: 398 274 147 411 5017
Fragments arranged by size:
5017 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2510 2540 2874 2904 1178
Size: 30 165 30 1107 30 334 30 4521
Fragments arranged by size:
4521 1107 334 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2515 2518 2617 2939 3094 1186
Size: 1329 3 99 322 155 4339
Fragments arranged by size:
4339 1329 322 155 99 3
BseYI C'CCAG_C
Cuts at: 2029 2362 2442 3189 3196 3210 3458 5450 5946
Size: 333 80 747 7 14 248 1992 496
Cuts at: 5946 2029
Size: 2330
Fragments arranged by size:
2330 1992 747 496 333 248 80 14
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2912 3029 1163
Size: 573 1176 117 4381
Fragments arranged by size:
4381 1176 573 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2622 2706 3697 4458 5486 1540
Size: 275 186 621 84 991 761 1028 2301
Fragments arranged by size:
2301 1028 991 761 621 275 186 84
BsiHKAI G_wGCw'C
Cuts at: 2103 2126 2699 3086 4308 4393 5464 2103
Size: 23 573 387 1222 85 1071 2886
Fragments arranged by size:
2886 1222 1071 573 387 85 23
BsmI GAATG_Cn'
Cuts at: 3758 3851 3758
Size: 93 6154
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2103 2126 2587 2699 2826 3086
Size: 399 246 67 23 461 112 127 260
Cuts at: 3086 3243 3566 4308 4393 5464 1391
Size: 157 323 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 461 399 323 260 246
157 127 112 85 67 23
BspEI T'CCGG_A
Cuts at: 5910 5910
Size: 6247
BspHI T'CATG_A
Cuts at: 4136 5866 4136
Size: 1730 4517
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2428 3220 3602 844
Size: 1584 792 382 3489
Fragments arranged by size:
3489 1584 792 382
BsrBI CCG'CTC
Cuts at: 1394 1823 2761 4134 1394
Size: 429 938 1373 3507
Fragments arranged by size:
3507 1373 938 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2795 4742 4916 65
Size: 1183 1547 1947 174 1396
Fragments arranged by size:
1947 1547 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2564 3114 4891 5078 1700
Size: 110 754 550 1777 187 2869
Fragments arranged by size:
2869 1777 754 550 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6247
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6247
BssSI C'ACGA_G
Cuts at: 4307 5319 4307
Size: 1012 5235
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6247
BstBI TT'CG_AA
Cuts at: 5051 5051
Size: 6247
BstEII G'GTnAC_C
Cuts at: 1184 3110 1184
Size: 1926 4321
BstXI CCAn_nnnn'nTGG
Cuts at: 3305 3305
Size: 6247
BstYI r'GATC_y
Cuts at: 1972 2541 3380 3577 3944 4329 4346 5798 5884
Size: 569 839 197 367 385 17 1452 86
Cuts at: 5884 5896 6242 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 839 569 385 367 346 197
86 17 12
BstZ17I GTA'TAC
Cuts at: 2417 2417
Size: 6247
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5508
Fragments arranged by size:
5508 683 56
BtsI GCAGTG_nn'
Cuts at: 3125 3200 3418 3763 4557 4577 3125
Size: 75 218 345 794 20 4795
Fragments arranged by size:
4795 794 345 218 75 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6247
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6212
DraI TTT'AAA
Cuts at: 3551 3551
Size: 6247
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5254 809
Size: 507 3938 1802
Fragments arranged by size:
3938 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2272 2574
Size: 54 1425 50 275 18 177 265 302
Cuts at: 2574 3295 3694 4583 6212 8
Size: 721 399 889 1629 43
Fragments arranged by size:
1629 1425 889 721 399 302 275 265
177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3694 1537
Size: 275 1882 4090
Fragments arranged by size:
4090 1882 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3402 4177 1353
Size: 2049 775 3423
Fragments arranged by size:
3423 2049 775
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2620 2864 3455 4834 5208 5354
Size: 1064 138 1031 244 591 1379 374 146
Cuts at: 5354 387
Size: 1280
Fragments arranged by size:
1379 1280 1064 1031 591 374 244 146
138
EcoICRI GAG'CTC
Cuts at: 2124 2124
Size: 6247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2055 2210 2445 2515 2612 2738 2772
Size: 393 423 155 235 70 97 126 34
Cuts at: 2772 4310 4907 5694 5962 1239
Size: 1538 597 787 268 1524
Fragments arranged by size:
1538 1524 787 597 423 393 268 235
155 126 97 70 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2424 2424
Size: 6247
EcoO109I rG'GnC_Cy
Cuts at: 2033 2134 2712 2823 3444 2033
Size: 101 578 111 621 4836
Fragments arranged by size:
4836 621 578 111 101
EcoRI G'AATT_C
Cuts at: 3556 3934 3556
Size: 378 5869
FspI TGC'GCA
Cuts at: 4753 4753
Size: 6247
HaeII r_GCGC'y
Cuts at: 1678 2092 2376 2817 2867 2918 3478 5394 1678
Size: 414 284 441 50 51 560 1916 2531
Fragments arranged by size:
2531 1916 560 441 414 284 51 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 2329 2361 2471
Size: 32 135 32 641 32 84 32 110
Cuts at: 2471 2503 2663 2695 2934 2966 4888 4920 4962
Size: 32 160 32 239 32 1922 32 42
Cuts at: 4962 4994 1373
Size: 32 2626
Fragments arranged by size:
2626 1922 641 239 160 135 110 84
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3591 3837 669
Size: 988 281 1653 246 3079
Fragments arranged by size:
3079 1653 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3046 748
Size: 2298 3949
HpaI GTT'AAC
Cuts at: 3837 3837
Size: 6247
KasI G'GCGC_C
Cuts at: 2088 2372 3474 2088
Size: 284 1102 4861
Fragments arranged by size:
4861 1102 284
KpnI G_GTAC'C
Cuts at: 3572 3572
Size: 6247
MfeI C'AATT_G
Cuts at: 3846 3846
Size: 6247
MluI A'CGCG_T
Cuts at: 6123 6123
Size: 6247
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 5361 5545 1305
Size: 939 3117 184 2007
Fragments arranged by size:
3117 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2274 3297 10
Size: 54 1425 343 442 1023 2960
Fragments arranged by size:
2960 1425 1023 442 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4205 4564 4723 518
Size: 648 3039 359 159 2042
Fragments arranged by size:
3039 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3076 3663 4340 5488 5733 1606
Size: 1470 587 677 1148 245 2120
Fragments arranged by size:
2120 1470 1148 677 587 245
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 6247
NarI GG'CG_CC
Cuts at: 2089 2373 3475 2089
Size: 284 1102 4861
Fragments arranged by size:
4861 1102 284
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4756
Fragments arranged by size:
4756 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6247
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 6247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6247
NotI GC'GGCC_GC
Cuts at: 3694 3694
Size: 6247
NspI r_CATG'y
Cuts at: 1132 1229 3138 3356 3605 5150 1132
Size: 97 1909 218 249 1545 2229
Fragments arranged by size:
2229 1909 1545 249 218 97
PasI CC'CwG_GG
Cuts at: 1348 2131 2827 3305 1348
Size: 783 696 478 4290
Fragments arranged by size:
4290 783 696 478
PciI A'CATG_T
Cuts at: 3134 5146 3134
Size: 2012 4235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2029 2137 2538 3268 3424 1108
Size: 921 108 401 730 156 3931
Fragments arranged by size:
3931 921 730 401 156 108
PmeI GTTT'AAAC
Cuts at: 3551 3551
Size: 6247
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4316 4348 5829 5861 4316
Size: 32 1481 32 4702
Fragments arranged by size:
4702 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2712 2712
Size: 6247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6247
PsiI TTA'TAA
Cuts at: 3817 3817
Size: 6247
PspOMI G'GGCC_C
Cuts at: 2032 2583 2822 2032
Size: 551 239 5457
Fragments arranged by size:
5457 551 239
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3272 3304 3272
Size: 32 6215
PstI C_TGCA'G
Cuts at: 830 2167 2871 3599 830
Size: 1337 704 728 3478
Fragments arranged by size:
3478 1337 728 704
PvuI CG_AT'CG
Cuts at: 2001 2706 2001
Size: 705 5542
PvuII CAG'CTG
Cuts at: 3076 3076
Size: 6247
SacI G_AGCT'C
Cuts at: 2126 2126
Size: 6247
SalI G'TCGA_C
Cuts at: 1936 3589 1936
Size: 1653 4594
SanDI GG'GwC_CC
Cuts at: 2712 2712
Size: 6247
SbfI CC_TGCA'GG
Cuts at: 3599 3599
Size: 6247
ScaI AGT'ACT
Cuts at: 4495 6207 4495
Size: 1712 4535
SfcI C'TryA_G
Cuts at: 826 1045 2163 2237 2867 3497 3595 4730 5411
Size: 219 1118 74 630 630 98 1135 681
Cuts at: 5411 5602 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1118 681 630 630 219 191
98 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 2582 2582
Size: 6247
SfoI GGC'GCC
Cuts at: 2090 2374 3476 2090
Size: 284 1102 4861
Fragments arranged by size:
4861 1102 284
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6247
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6247
SmaI CCC'GGG
Cuts at: 1872 3574 1872
Size: 1702 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2787 3509 4351 5252 5514 820
Size: 197 926 844 722 842 901 262 1553
Fragments arranged by size:
1553 926 901 844 842 722 262 197
SnaBI TAC'GTA
Cuts at: 493 4038 493
Size: 3545 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6247
SphI G_CATG'C
Cuts at: 1132 3605 1132
Size: 2473 3774
SspI AAT'ATT
Cuts at: 5 52 4171 5
Size: 47 4119 2081
Fragments arranged by size:
4119 2081 47
StuI AGG'CCT
Cuts at: 1517 2430 1517
Size: 913 5334
StyI C'CwwG_G
Cuts at: 513 1065 2004 2317 2355 2571 3469 3686 513
Size: 552 939 313 38 216 898 217 3074
Fragments arranged by size:
3074 939 898 552 313 217 216 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 2239 2608 3098 4274 4291 4444
Size: 1255 504 155 369 490 1176 17 153
Cuts at: 4444 4629 5074 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1176 504 490 445 369 185
155 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4493 6205 96
Size: 274 80 33 51 1443 2516 1712 138
Fragments arranged by size:
2516 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 6207 6239 2874
Size: 32 3301 32 2882
Fragments arranged by size:
3301 2882 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6247
XbaI T'CTAG_A
Cuts at: 3583 3583
Size: 6247
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2181 2267 2181
Size: 86 6161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6247
XmaI C'CCGG_G
Cuts at: 1870 3572 1870
Size: 1702 4545
XmnI GAAnn'nnTTC
Cuts at: 3485 4376 3485
Size: 891 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4055 276
Size: 53 83 186 936 2521 2468
Fragments arranged by size:
2521 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BfrBI BsiWI BsmBI Bsu36I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI RsrII SacII SapI SexAI SrfI SwaI