Restriction map for
pFN21AE1955
(Circular) (Six-base) MAPSORT of: pFN21AE1955.seq Check: 7958 from: 1 to: 6244
pFN21AE1955 6244 bp
With 182 enzymes: SgfI *
March 30, 2011 18:12 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3587 1937
Size: 1650 4594
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2411 2969 3189 4307 5691 5959 1632
Size: 779 558 220 1118 1384 268 1917
Fragments arranged by size:
1917 1384 1118 779 558 268 220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 5143 6120 1203
Size: 3940 977 1327
Fragments arranged by size:
3940 1327 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 2458 4973 2458
Size: 2515 3729
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 2035 2067 2134 2359 3987 5559 2035
Size: 32 67 225 1628 1572 2720
Fragments arranged by size:
2720 1628 1572 225 67 32
ApaI G_GGCC'C
Cuts at: 2076 2076
Size: 6244
ApaLI G'TGCA_C
Cuts at: 3107 4301 5457 3107
Size: 1194 1156 3894
Fragments arranged by size:
3894 1194 1156
ApoI r'AATT_y
Cuts at: 1493 2768 2924 3553 3779 3931 6040 1493
Size: 1275 156 629 226 152 2109 1697
Fragments arranged by size:
2109 1697 1275 629 226 156 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2194 2381 2530 3027 3413 3569 1870
Size: 73 251 187 149 497 386 156 4545
Fragments arranged by size:
4545 497 386 251 187 156 149 73
BamHI G'GATC_C
Cuts at: 3574 3941 3574
Size: 367 5877
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2534 3141 3565 5020 618
Size: 325 217 627 747 607 424 1455 1842
Fragments arranged by size:
1842 1455 747 627 607 424 325 217
BanII G_rGCy'C
Cuts at: 1391 2076 2153 2271 2376 3563 1391
Size: 685 77 118 105 1187 4072
Fragments arranged by size:
4072 1187 685 118 105 77
BbeI G_GCGC'C
Cuts at: 2538 3145 2538
Size: 607 5637
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2345 2379 2487 2521 4435
Size: 34 50 34 324 34 108 34 1914
Cuts at: 4435 4469 1903
Size: 34 3678
Fragments arranged by size:
3678 1914 324 108 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6244
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3045 4855 136
Size: 107 122 71 631 605 1373 1810 1525
Fragments arranged by size:
1810 1525 1373 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2250 6239 2250
Size: 3989 2255
BlpI GC'TnA_GC
Cuts at: 2990 3661 2990
Size: 671 5573
Bme1580I G_kGCm'C
Cuts at: 1790 2076 3111 4305 5461 1790
Size: 286 1035 1194 1156 2573
Fragments arranged by size:
2573 1194 1156 1035 286
BmgBI CAC'GTC
Cuts at: 1318 3019 3184 1318
Size: 1701 165 4378
Fragments arranged by size:
4378 1701 165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3166 3975 4933
Size: 416 53 83 399 24 1749 809 958
Cuts at: 4933 442
Size: 1753
Fragments arranged by size:
1753 1749 958 809 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3118 3181 4904 1239
Size: 1879 63 1723 2579
Fragments arranged by size:
2579 1879 1723 63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2201 4369 5234 5532 2201
Size: 2168 865 298 2913
Fragments arranged by size:
2913 2168 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3199 4907 882
Size: 617 1700 1708 2219
Fragments arranged by size:
2219 1708 1700 617
BsaAI yAC'GTr
Cuts at: 493 1204 3112 4035 493
Size: 711 1908 923 2702
Fragments arranged by size:
2702 1908 923 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2459 2535 3142 4051
Size: 53 83 186 936 926 76 607 909
Cuts at: 4051 4433 5068 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 926 909 635 607 382 186
83 76 53
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 3003 3033 1178
Size: 30 165 30 837 30 733 30 4389
Fragments arranged by size:
4389 837 733 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2182 2351 1186
Size: 996 169 5079
Fragments arranged by size:
5079 996 169
BseYI C'CCAG_C
Cuts at: 2014 2134 2263 2511 5447 5943 2014
Size: 120 129 248 2936 496 2315
Fragments arranged by size:
2936 2315 496 248 129 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2080 2276 3407 1163
Size: 573 344 196 1131 4000
Fragments arranged by size:
4000 1131 573 344 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2158 3694 4455 5483 1540
Size: 275 186 111 46 1536 761 1028 2301
Fragments arranged by size:
2301 1536 1028 761 275 186 111 46
BsiHKAI G_wGCw'C
Cuts at: 3111 4305 4390 5461 3111
Size: 1194 85 1071 3894
Fragments arranged by size:
3894 1194 1071 85
BsmI GAATG_Cn'
Cuts at: 3341 3370 3755 3848 3341
Size: 29 385 93 5737
Fragments arranged by size:
5737 385 93 29
BsmBI CGTCTCn'nnnn_
Cuts at: 3045 3045
Size: 6244
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2153 2271 2376 3111 3563 4305
Size: 399 286 77 118 105 735 452 742
Cuts at: 4305 4390 5461 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 735 452 399 286 118
105 85 77
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 2649 4133 5863 2649
Size: 1484 1730 3030
Fragments arranged by size:
3030 1730 1484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3599 844
Size: 2755 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2548 4131 1394
Size: 429 725 1583 3507
Fragments arranged by size:
3507 1583 725 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3294 4739 4913 65
Size: 1183 2046 1445 174 1396
Fragments arranged by size:
2046 1445 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2208 2277 3138 4888 5075 1700
Size: 110 398 69 861 1750 187 2869
Fragments arranged by size:
2869 1750 861 398 187 110 69
BsrGI T'GTAC_A
Cuts at: 96 2421 96
Size: 2325 3919
BssHII G'CGCG_C
Cuts at: 1925 2610 1925
Size: 685 5559
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2067 2359 794
Size: 1273 292 4679
Fragments arranged by size:
4679 1273 292
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 2250 2416 2576 3574 3941 4326 4343 5795
Size: 278 166 160 998 367 385 17 1452
Cuts at: 5795 5881 5893 6239 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 998 385 367 346 278 166
160 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2742 2742
Size: 6244
BtgI C'CryG_G
Cuts at: 513 1065 2106 513
Size: 552 1041 4651
Fragments arranged by size:
4651 1041 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 2036 3226 3760 4554 4574 2036
Size: 1190 534 794 20 3706
Fragments arranged by size:
3706 1190 794 534 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 2806 3548 2806
Size: 742 5502
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3153 5251 809
Size: 507 1837 2098 1802
Fragments arranged by size:
2098 1837 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2155 2937
Size: 54 1425 50 275 18 279 46 782
Cuts at: 2937 3691 4580 6209 8
Size: 754 889 1629 43
Fragments arranged by size:
1629 1425 889 782 754 279 275 54
50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2155 3691 1537
Size: 275 297 46 1536 4090
Fragments arranged by size:
4090 1536 297 275 46
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 4174 1353
Size: 1058 1763 3423
Fragments arranged by size:
3423 1763 1058
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2035 2425 4831 5205 5351 387
Size: 1064 138 446 390 2406 374 146 1280
Fragments arranged by size:
2406 1280 1064 446 390 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2411 2969 3118 3181 3189 4307 4904
Size: 393 779 558 149 63 8 1118 597
Cuts at: 4904 5691 5959 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1118 787 779 597 558 393 268
149 63 8
EcoO109I rG'GnC_Cy
Cuts at: 2073 2073
Size: 6244
EcoRI G'AATT_C
Cuts at: 2768 3553 3931 2768
Size: 785 378 5081
Fragments arranged by size:
5081 785 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2269 2743 3091 2269
Size: 474 348 5422
Fragments arranged by size:
5422 474 348
FspI TGC'GCA
Cuts at: 3116 4750 3116
Size: 1634 4610
FspAI rTGC'GCAy
Cuts at: 3116 3116
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 2324 2538 2625 3145 5391 1678
Size: 646 214 87 520 2246 2531
Fragments arranged by size:
2531 2246 646 520 214 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2240 2272 2444 2476 3003
Size: 32 135 32 668 32 172 32 527
Cuts at: 3003 3035 3145 3177 3470 3502 4885 4917 4959
Size: 32 110 32 293 32 1383 32 42
Cuts at: 4959 4991 1373
Size: 32 2626
Fragments arranged by size:
2626 1383 668 527 293 172 135 110
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3588 3834 669
Size: 988 281 1650 246 3079
Fragments arranged by size:
3079 1650 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6244
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KasI G'GCGC_C
Cuts at: 2534 3141 2534
Size: 607 5637
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2326 2442 2862 5358 5542 1305
Size: 1021 116 420 2496 184 2007
Fragments arranged by size:
2496 2007 1021 420 184 116
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4422
Fragments arranged by size:
4422 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 3363 4202 4561 4720 518
Size: 648 1482 715 839 359 159 2042
Fragments arranged by size:
2042 1482 839 715 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2083 2102 2108 2131 2359 2586 2989
Size: 458 19 19 6 23 228 227 403
Cuts at: 2989 3660 4337 5485 5730 1606
Size: 671 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 671 458 403 245 228
227 23 19 19 6
NaeI GCC'GGC
Cuts at: 3140 3140
Size: 6244
NarI GG'CG_CC
Cuts at: 2535 3142 2535
Size: 607 5637
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5692
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NgoMIV G'CCGG_C
Cuts at: 3138 3138
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 2109 3691 2109
Size: 1582 4662
NspI r_CATG'y
Cuts at: 1132 1229 3602 5147 1132
Size: 97 2373 1545 2229
Fragments arranged by size:
2373 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6244
PciI A'CATG_T
Cuts at: 5143 5143
Size: 6244
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3184 3274 1108
Size: 2076 90 4078
Fragments arranged by size:
4078 2076 90
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PmlI CAC'GTG
Cuts at: 3112 3112
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2705 2737 4313 4345 5826 5858 2705
Size: 32 1576 32 1481 32 3091
Fragments arranged by size:
3091 1576 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2392 1613
Size: 779 5465
PsiI TTA'TAA
Cuts at: 3814 3814
Size: 6244
PspOMI G'GGCC_C
Cuts at: 2072 2072
Size: 6244
PstI C_TGCA'G
Cuts at: 830 2088 2767 3242 3596 830
Size: 1258 679 475 354 3478
Fragments arranged by size:
3478 1258 679 475 354
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
PvuII CAG'CTG
Cuts at: 2064 2131 2359 2989 2064
Size: 67 228 630 5319
Fragments arranged by size:
5319 630 228 67
SacII CC_GC'GG
Cuts at: 2109 2109
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 3242 3596 3242
Size: 354 5890
ScaI AGT'ACT
Cuts at: 4492 6204 4492
Size: 1712 4532
SexAI A'CCwGG_T
Cuts at: 2664 3122 2664
Size: 458 5786
SfcI C'TryA_G
Cuts at: 826 1045 2084 2763 3238 3592 4727 5408 5599
Size: 219 1039 679 475 354 1135 681 191
Cuts at: 5599 826
Size: 1471
Fragments arranged by size:
1471 1135 1039 681 679 475 354 219
191
SfoI GGC'GCC
Cuts at: 2536 3143 2536
Size: 607 5637
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 2383 2532 3571 1872
Size: 511 149 1039 4545
Fragments arranged by size:
4545 1039 511 149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 4348 5249 5511 820
Size: 197 926 273 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 273 262 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 3039 1517
Size: 1522 4722
StyI C'CwwG_G
Cuts at: 513 1065 3303 3393 3683 513
Size: 552 2238 90 290 3074
Fragments arranged by size:
3074 2238 552 290 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3316 4271 4288 4441 4626 5071 325
Size: 1255 1736 955 17 153 185 445 1498
Fragments arranged by size:
1736 1498 1255 955 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2421 4490 6202
Size: 274 80 33 51 1443 444 2069 1712
Cuts at: 6202 96
Size: 138
Fragments arranged by size:
2069 1712 1443 444 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6204 6236 6204
Size: 32 6212
Tth111I GACn'n_nGTC
Cuts at: 1316 2645 1316
Size: 1329 4915
XbaI T'CTAG_A
Cuts at: 3580 3580
Size: 6244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 2381 2530 3569 1870
Size: 511 149 1039 4545
Fragments arranged by size:
4545 1039 511 149
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI NruI NsiI PacI PfoI PpuMI PspXI
PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI