Restriction map for
pFN21AE1954
(Circular) (Six-base) MAPSORT of: pFN21AE1954.seq Check: 9382 from: 1 to: 6235
pFN21AE1954 6235 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3578 1937
Size: 1641 4594
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2337 2409 3267 4298 5682 5950 1632
Size: 705 72 858 1031 1384 268 1917
Fragments arranged by size:
1917 1384 1031 858 705 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2613 3122 1184
Size: 1429 509 4297
Fragments arranged by size:
4297 1429 509
AlwNI CAG_nnn'CTG
Cuts at: 3978 5550 3978
Size: 1572 4663
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 2146 2744 2936 3544 3770 3922 6031 1493
Size: 653 598 192 608 226 152 2109 1697
Fragments arranged by size:
2109 1697 653 608 598 226 192 152
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 3560 1870
Size: 73 1617 4545
Fragments arranged by size:
4545 1617 73
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2188 2210 3556 5011 618
Size: 325 217 627 401 22 1346 1455 1842
Fragments arranged by size:
1842 1455 1346 627 401 325 217 22
BanII G_rGCy'C
Cuts at: 1391 2056 2564 2820 2976 3554 1391
Size: 665 508 256 156 578 4072
Fragments arranged by size:
4072 665 578 508 256 156
BbeI G_GCGC'C
Cuts at: 2214 2214
Size: 6235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 2404 1548
Size: 856 5379
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2225 6230 2225
Size: 4005 2230
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2106 4296 5452 1790
Size: 316 2190 1156 2573
Fragments arranged by size:
2573 2190 1156 316
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2270 3966 4924
Size: 416 53 83 399 24 853 1696 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1753 1696 958 853 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2040 2847 4895 1239
Size: 801 807 2048 2579
Fragments arranged by size:
2579 2048 807 801
Bpu10I CC'TnA_GC
Cuts at: 2068 2068
Size: 6235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4360 5225 5523 4360
Size: 865 298 5072
Fragments arranged by size:
5072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4898 882
Size: 617 3399 2219
Fragments arranged by size:
3399 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2211 4042 4424 5059
Size: 53 83 186 936 678 1831 382 635
Cuts at: 5059 275
Size: 1451
Fragments arranged by size:
1831 1451 936 678 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2154 4668 5066 5340 5487 5898 2154
Size: 2514 398 274 147 411 2491
Fragments arranged by size:
2514 2491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2007 2037 1178
Size: 30 165 30 604 30 5376
Fragments arranged by size:
5376 604 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 1186
Size: 829 5406
BseYI C'CCAG_C
Cuts at: 3271 3523 5438 5934 3271
Size: 252 1915 496 3572
Fragments arranged by size:
3572 1915 496 252
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2883 1163
Size: 573 1147 4515
Fragments arranged by size:
4515 1147 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2100 2313 3685 4446 5474 1540
Size: 275 186 99 213 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 213 186 99
BsiHKAI G_wGCw'C
Cuts at: 2017 2564 2820 2976 4296 4381 5452 2017
Size: 547 256 156 1320 85 1071 2800
Fragments arranged by size:
2800 1320 1071 547 256 156 85
BsmI GAATG_Cn'
Cuts at: 3746 3839 3746
Size: 93 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2017 2056 2106 2564 2820 2976 3554
Size: 399 227 39 50 458 256 156 578
Cuts at: 3554 4296 4381 5452 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 458 399 256 227
156 85 50 39
BspEI T'CCGG_A
Cuts at: 2154 5898 2154
Size: 3744 2491
BspHI T'CATG_A
Cuts at: 2614 2956 4124 5854 2614
Size: 342 1168 1730 2995
Fragments arranged by size:
2995 1730 1168 342
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3590 844
Size: 2746 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2309 4122 1394
Size: 429 486 1813 3507
Fragments arranged by size:
3507 1813 486 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2592 2784 3147 3208 4730 4904 65
Size: 1183 1344 192 363 61 1522 174 1396
Fragments arranged by size:
1522 1396 1344 1183 363 192 174 61
BsrFI r'CCGG_y
Cuts at: 1700 1810 4879 5066 1700
Size: 110 3069 187 2869
Fragments arranged by size:
3069 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 2030 1925
Size: 105 6130
BssSI C'ACGA_G
Cuts at: 2045 2417 4295 5307 2045
Size: 372 1878 1012 2973
Fragments arranged by size:
2973 1878 1012 372
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3416 794
Size: 2622 3613
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstXI CCAn_nnnn'nTGG
Cuts at: 2333 3271 2333
Size: 938 5297
BstYI r'GATC_y
Cuts at: 1972 2225 3565 3932 4317 4334 5786 5872 5884
Size: 253 1340 367 385 17 1452 86 12
Cuts at: 5884 6230 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1340 385 367 346 253 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3513 3513
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 3147 513
Size: 552 2082 3601
Fragments arranged by size:
3601 2082 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 2832 2922 3751 4545 4565 2832
Size: 90 829 794 20 4502
Fragments arranged by size:
4502 829 794 90 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6200
DraI TTT'AAA
Cuts at: 2853 3539 2853
Size: 686 5549
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 2310 3682
Size: 54 1425 50 275 18 267 213 1372
Cuts at: 3682 4571 6200 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1372 889 275 267 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2097 2310 3682 1537
Size: 275 285 213 1372 4090
Fragments arranged by size:
4090 1372 285 275 213
EarI CTCTTCn'nnn_
Cuts at: 1353 4165 1353
Size: 2812 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4822 5196 5342 387
Size: 1064 138 3233 374 146 1280
Fragments arranged by size:
3233 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2562 2818 2974 2562
Size: 256 156 5823
Fragments arranged by size:
5823 256 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2040 2337 2409 2847 3267 4298 4895
Size: 393 408 297 72 438 420 1031 597
Cuts at: 4895 5682 5950 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1031 787 597 438 420 408 393
297 268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 3054 3390 3054
Size: 336 5899
EcoRI G'AATT_C
Cuts at: 3544 3922 3544
Size: 378 5857
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 2214 5382 1678
Size: 536 3168 2531
Fragments arranged by size:
3168 2531 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 3057 3089 4876
Size: 32 135 32 1079 32 374 32 1787
Cuts at: 4876 4908 4950 4982 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1787 1079 374 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3579 3825 669
Size: 988 281 1641 246 3079
Fragments arranged by size:
3079 1641 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2286 748
Size: 1538 4697
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KasI G'GCGC_C
Cuts at: 2210 2210
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 2487 3834 2487
Size: 1347 4888
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3172 5349 5533 1305
Size: 1867 2177 184 2007
Fragments arranged by size:
2177 2007 1867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2585 3435 4193 4552 4711 518
Size: 648 1419 850 758 359 159 2042
Fragments arranged by size:
2042 1419 850 758 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2315 2825 3651 4328 5476 5721 1606
Size: 709 510 826 677 1148 245 2120
Fragments arranged by size:
2120 1148 826 709 677 510 245
NarI GG'CG_CC
Cuts at: 2211 2211
Size: 6235
NcoI C'CATG_G
Cuts at: 513 1065 3147 513
Size: 552 2082 3601
Fragments arranged by size:
3601 2082 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 2310 3682 2310
Size: 1372 4863
NspI r_CATG'y
Cuts at: 1132 1229 2269 3523 3593 5138 1132
Size: 97 1040 1254 70 1545 2229
Fragments arranged by size:
2229 1545 1254 1040 97 70
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6235
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3166 1108
Size: 2058 4177
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PstI C_TGCA'G
Cuts at: 830 3005 3587 830
Size: 2175 582 3478
Fragments arranged by size:
3478 2175 582
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2825 2825
Size: 6235
SacI G_AGCT'C
Cuts at: 2564 2820 2976 2564
Size: 256 156 5823
Fragments arranged by size:
5823 256 156
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 2622 4483 6195 2622
Size: 1861 1712 2662
Fragments arranged by size:
2662 1861 1712
SfcI C'TryA_G
Cuts at: 826 1045 2636 3001 3583 4718 5399 5590 826
Size: 219 1591 365 582 1135 681 191 1471
Fragments arranged by size:
1591 1471 1135 681 582 365 219 191
SfoI GGC'GCC
Cuts at: 2212 2212
Size: 6235
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4339 5240 5502 820
Size: 197 926 2396 901 262 1553
Fragments arranged by size:
2396 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 2269 3593 1132
Size: 1137 1324 3774
Fragments arranged by size:
3774 1324 1137
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6235
StyI C'CwwG_G
Cuts at: 513 1065 3147 3674 513
Size: 552 2082 527 3074
Fragments arranged by size:
3074 2082 552 527
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2078 3042 4262 4279 4432 4617 5062
Size: 1255 498 964 1220 17 153 185 445
Cuts at: 5062 325
Size: 1498
Fragments arranged by size:
1498 1255 1220 964 498 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2620 4481 6193
Size: 274 80 33 51 1443 643 1861 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
1861 1712 1443 643 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2007 2039 6195 6227 2007
Size: 32 4156 32 2015
Fragments arranged by size:
4156 2015 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 3224 4364 3224
Size: 1140 5095
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI