Restriction map for
pFN21AE1949
(Circular) (Six-base) MAPSORT of: pFN21AE1949.seq Check: 4241 from: 1 to: 6217
pFN21AE1949 6217 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2590 2683 2590
Size: 93 6124
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4280 5664 5932 1632
Size: 2648 1384 268 1917
Fragments arranged by size:
2648 1917 1384 268
AfeI AGC'GCT
Cuts at: 2782 2782
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 3514 5048 3514
Size: 1534 4683
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2590 1184
Size: 1406 4811
AlwNI CAG_nnn'CTG
Cuts at: 2254 2836 3057 3960 5532 2254
Size: 582 221 903 1572 2939
Fragments arranged by size:
2939 1572 903 582 221
ApaLI G'TGCA_C
Cuts at: 2565 4274 5430 2565
Size: 1709 1156 3352
Fragments arranged by size:
3352 1709 1156
ApoI r'AATT_y
Cuts at: 1493 2487 3526 3752 3904 6013 1493
Size: 994 1039 226 152 2109 1697
Fragments arranged by size:
2109 1697 1039 994 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3542 1870
Size: 73 1599 4545
Fragments arranged by size:
4545 1599 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3149 3182 3149
Size: 33 6184
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3538 4993 618
Size: 325 217 627 1751 1455 1842
Fragments arranged by size:
1842 1751 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2268 2649 3140 3268 3444 3536 1391
Size: 877 381 491 128 176 92 4072
Fragments arranged by size:
4072 877 491 381 176 128 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2197 928
Size: 971 298 4948
Fragments arranged by size:
4948 971 298
BbvCI CC'TCA_GC
Cuts at: 2424 2424
Size: 6217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2513 2894
Size: 107 122 71 631 605 337 504 381
Cuts at: 2894 4828 136
Size: 1934 1525
Fragments arranged by size:
1934 1525 631 605 504 381 337 122
107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2095 3634 2095
Size: 1539 4678
Bme1580I G_kGCm'C
Cuts at: 1790 2135 2569 2830 4278 5434 1790
Size: 345 434 261 1448 1156 2573
Fragments arranged by size:
2573 1448 1156 434 345 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2378 2381 3172 3352 3496 4877 1239
Size: 1139 3 791 180 144 1381 2579
Fragments arranged by size:
2579 1381 1139 791 180 144 3
Bpu10I CC'TnA_GC
Cuts at: 2424 2424
Size: 6217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2150 2453 2480 4342 5207 5505 2150
Size: 303 27 1862 865 298 2862
Fragments arranged by size:
2862 1862 865 303 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2373 4024 4406 5041
Size: 53 83 186 936 840 1651 382 635
Cuts at: 5041 275
Size: 1451
Fragments arranged by size:
1651 1451 936 840 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3514 4650 5048 5322 5469 5880 3514
Size: 1136 398 274 147 411 3851
Fragments arranged by size:
3851 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2864 2894 3147 3177 1178
Size: 30 165 30 1461 30 253 30 4218
Fragments arranged by size:
4218 1461 253 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 2035 2362 2430 1186
Size: 846 3 327 68 4973
Fragments arranged by size:
4973 846 327 68 3
BseYI C'CCAG_C
Cuts at: 2247 2272 2665 3109 3139 3145 5420 5916 2247
Size: 25 393 444 30 6 2275 496 2548
Fragments arranged by size:
2548 2275 496 444 393 30 25 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 3667 4428 5456 1540
Size: 275 186 1432 234 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 2268 2295 2569 2649 2723 3203 4278 4363 5434
Size: 27 274 80 74 480 1075 85 1071
Cuts at: 5434 2268
Size: 3051
Fragments arranged by size:
3051 1075 1071 480 274 85 80 74
27
BsmI GAATG_Cn'
Cuts at: 2290 3728 3821 2290
Size: 1438 93 4686
Fragments arranged by size:
4686 1438 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2268 2295 2569 2649 2723 2830
Size: 399 345 133 27 274 80 74 107
Cuts at: 2830 3140 3203 3268 3444 3536 4278 4363 5434
Size: 310 63 65 176 92 742 85 1071
Cuts at: 5434 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 345 310 274 176
133 107 92 85 80 74 65 63
27
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2073 2590 2683 3572 844
Size: 1229 517 93 889 3489
Fragments arranged by size:
3489 1229 889 517 93
BsrBI CCG'CTC
Cuts at: 1394 1823 3393 4104 1394
Size: 429 1570 711 3507
Fragments arranged by size:
3507 1570 711 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3514 4861 5048 1700
Size: 110 1704 1347 187 2869
Fragments arranged by size:
2869 1704 1347 187 110
BsrGI T'GTAC_A
Cuts at: 96 2880 96
Size: 2784 3433
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 3068 4277 5289 3068
Size: 1209 1012 3996
Fragments arranged by size:
3996 1209 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 794
Size: 1640 4577
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2084 2242 2909 2084
Size: 158 667 5392
Fragments arranged by size:
5392 667 158
BstYI r'GATC_y
Cuts at: 1972 2454 3547 3914 4299 4316 5768 5854 5866
Size: 482 1093 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1093 482 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2502 2821 2902 3487 513
Size: 552 939 498 319 81 585 3243
Fragments arranged by size:
3243 939 585 552 498 319 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2788 2951 487
Size: 683 56 1562 163 3753
Fragments arranged by size:
3753 1562 683 163 56
BtsI GCAGTG_nn'
Cuts at: 2985 3733 4527 4547 2985
Size: 748 794 20 4655
Fragments arranged by size:
4655 794 748 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3664 4553
Size: 54 1425 50 275 18 675 1159 889
Cuts at: 4553 6182 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1159 889 675 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2654 3392 4147 1353
Size: 1301 738 755 3423
Fragments arranged by size:
3423 1301 755 738
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2743 4804 5178 5324 387
Size: 1064 138 1154 2061 374 146 1280
Fragments arranged by size:
2061 1280 1154 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2266 2647 2266
Size: 381 5836
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2378 2381 3172 3352 3496 4280 4877
Size: 393 746 3 791 180 144 784 597
Cuts at: 4877 5664 5932 1239
Size: 787 268 1524
Fragments arranged by size:
1524 791 787 784 746 597 393 268
180 144 3
EcoO109I rG'GnC_Cy
Cuts at: 2887 2887
Size: 6217
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2784 3195 5364 1678
Size: 1106 411 2169 2531
Fragments arranged by size:
2531 2169 1106 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373
Size: 32 135 32 3286 32 42 32 2626
Fragments arranged by size:
3286 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3259 3561 3807 669
Size: 988 281 1321 302 246 3079
Fragments arranged by size:
3079 1321 988 302 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 2405 3816 2405
Size: 1411 4806
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2127 2850 5331 5515 1305
Size: 822 723 2481 184 2007
Fragments arranged by size:
2481 2007 822 723 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2572 4175 4534 4693 518
Size: 648 1406 1603 359 159 2042
Fragments arranged by size:
2042 1603 1406 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2160 2251 2281 2434 2611 2665 2836 3109
Size: 554 91 30 153 177 54 171 273
Cuts at: 3109 3149 3633 4310 5458 5703 1606
Size: 40 484 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 554 484 273 245 177
171 153 91 54 40 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 2502 2821 2902 3487 513
Size: 552 939 498 319 81 585 3243
Fragments arranged by size:
3243 939 585 552 498 319 81
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3244 1348
Size: 1896 4321
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6217
PfoI T'CCnGG_A
Cuts at: 2355 2355
Size: 6217
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3086 3118 3086
Size: 32 6185
PstI C_TGCA'G
Cuts at: 830 2034 2328 3154 3336 3569 830
Size: 1204 294 826 182 233 3478
Fragments arranged by size:
3478 1204 826 294 233 182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2160 2281 2434 2611 2665 3149 2160
Size: 121 153 177 54 484 5228
Fragments arranged by size:
5228 484 177 153 121 54
SacI G_AGCT'C
Cuts at: 2268 2649 2268
Size: 381 5836
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SapI GCTCTTCn'nnn_
Cuts at: 2654 2654
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2030 2324 3150 3332 3565 4700 5381
Size: 219 985 294 826 182 233 1135 681
Cuts at: 5381 5572 826
Size: 191 1471
Fragments arranged by size:
1471 1135 985 826 681 294 233 219
191 182
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2165 2459 2468 4321 5222 5484
Size: 197 926 222 294 9 1853 901 262
Cuts at: 5484 820
Size: 1553
Fragments arranged by size:
1853 1553 926 901 294 262 222 197
9
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2330 3384 1517
Size: 813 1054 4350
Fragments arranged by size:
4350 1054 813
StyI C'CwwG_G
Cuts at: 513 1065 2004 2376 2502 2821 2902 3487 3656
Size: 552 939 372 126 319 81 585 169
Cuts at: 3656 513
Size: 3074
Fragments arranged by size:
3074 939 585 552 372 319 169 126
81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 4261 4414 4599 5044 325
Size: 1255 2664 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2880 4463 6175
Size: 274 80 33 51 1443 903 1583 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1712 1583 1443 903 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2692 2724 3147 3179 3260 3292 6177 6209 2692
Size: 32 423 32 81 32 2885 32 2700
Fragments arranged by size:
2885 2700 423 81 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2157 2509 2157
Size: 352 5865
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PsrI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI