Restriction map for
pFN21AE1946
(Circular) (Six-base) MAPSORT of: pFN21AE1946.seq Check: 6237 from: 1 to: 6208
pFN21AE1946 6208 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3404 3404
Size: 6208
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3551 1937
Size: 1614 4594
Acc65I G'GTAC_C
Cuts at: 2040 3529 2040
Size: 1489 4719
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2161 2462 2813 4271 5655 5923 1632
Size: 529 301 351 1458 1384 268 1917
Fragments arranged by size:
1917 1458 1384 529 351 301 268
AflII C'TTAA_G
Cuts at: 820 1017 3191 820
Size: 197 2174 3837
Fragments arranged by size:
3837 2174 197
AflIII A'CryG_T
Cuts at: 1203 2670 5107 6084 1203
Size: 1467 2437 977 1327
Fragments arranged by size:
2437 1467 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 3379 4937 3379
Size: 1558 4650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6208
AlwNI CAG_nnn'CTG
Cuts at: 2420 3358 3951 5523 2420
Size: 938 593 1572 3105
Fragments arranged by size:
3105 1572 938 593
ApaI G_GGCC'C
Cuts at: 2466 3151 2466
Size: 685 5523
ApaLI G'TGCA_C
Cuts at: 4265 5421 4265
Size: 1156 5052
ApoI r'AATT_y
Cuts at: 1493 2063 3517 3743 3895 6004 1493
Size: 570 1454 226 152 2109 1697
Fragments arranged by size:
2109 1697 1454 570 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2459 3377 3533 1870
Size: 73 516 918 156 4545
Fragments arranged by size:
4545 918 516 156 73
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2084 2240 2401 3529
Size: 325 217 627 253 44 156 161 1128
Cuts at: 3529 4984 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1128 627 325 253 217 161
156 44
BanII G_rGCy'C
Cuts at: 1391 2460 2466 2478 3151 3333 3527 1391
Size: 1069 6 12 673 182 194 4072
Fragments arranged by size:
4072 1069 673 194 182 12 6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2167 928
Size: 971 268 4969
Fragments arranged by size:
4969 971 268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4100 5310 4100
Size: 1210 4998
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6208
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2814 3155 3412
Size: 107 122 71 631 605 1142 341 257
Cuts at: 3412 4819 136
Size: 1407 1525
Fragments arranged by size:
1525 1407 1142 631 605 341 257 122
107 71
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2089 2466 2689 3151 3408 4269 5425 1790
Size: 299 377 223 462 257 861 1156 2573
Fragments arranged by size:
2573 1156 861 462 377 299 257 223
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6208
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3939 4897 442
Size: 416 53 83 399 24 2522 958 1753
Fragments arranged by size:
2522 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2711 2957 2963 3347 4868 1239
Size: 1472 246 6 384 1521 2579
Fragments arranged by size:
2579 1521 1472 384 246 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3290 4333 5198 5496 3290
Size: 1043 865 298 4002
Fragments arranged by size:
4002 1043 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2098 4871 882
Size: 617 599 2773 2219
Fragments arranged by size:
2773 2219 617 599
BsaAI yAC'GTr
Cuts at: 493 1204 2671 3999 493
Size: 711 1467 1328 2702
Fragments arranged by size:
2702 1467 1328 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4015 4397 5032 275
Size: 53 83 186 936 2482 382 635 1451
Fragments arranged by size:
2482 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4641 5039 5313 5460 5871 4641
Size: 398 274 147 411 4978
Fragments arranged by size:
4978 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5983
Fragments arranged by size:
5983 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 1186
Size: 826 5382
BseYI C'CCAG_C
Cuts at: 2426 2707 2787 2810 2874 2925 3179 3243 5411
Size: 281 80 23 64 51 254 64 2168
Cuts at: 5411 5907 2426
Size: 496 2727
Fragments arranged by size:
2727 2168 496 281 254 80 64 64
51 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2056 1163
Size: 573 320 5315
Fragments arranged by size:
5315 573 320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2818 2995 3658 4419 5447 1540
Size: 275 186 817 177 663 761 1028 2301
Fragments arranged by size:
2301 1028 817 761 663 275 186 177
BsiHKAI G_wGCw'C
Cuts at: 4269 4354 5425 4269
Size: 85 1071 5052
Fragments arranged by size:
5052 1071 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2089 2460 2466 2478 2689 3151 3333
Size: 399 299 371 6 12 211 462 182
Cuts at: 3333 3408 3527 4269 4354 5425 1391
Size: 75 119 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 462 399 371 299 211
182 119 85 75 12 6
BspEI T'CCGG_A
Cuts at: 5871 5871
Size: 6208
BspHI T'CATG_A
Cuts at: 4097 5827 4097
Size: 1730 4478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2998 3404 3563 844
Size: 2154 406 159 3489
Fragments arranged by size:
3489 2154 406 159
BsrBI CCG'CTC
Cuts at: 1394 1823 3087 4095 1394
Size: 429 1264 1008 3507
Fragments arranged by size:
3507 1264 1008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3311 4703 4877 65
Size: 1183 2063 1392 174 1396
Fragments arranged by size:
2063 1396 1392 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2817 2824 4852 5039 1700
Size: 110 1007 7 2028 187 2869
Fragments arranged by size:
2869 2028 1007 187 110 7
BsrGI T'GTAC_A
Cuts at: 96 2532 96
Size: 2436 3772
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 2469 2513 3014 4268 5280 2469
Size: 44 501 1254 1012 3397
Fragments arranged by size:
3397 1254 1012 501 44
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6208
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6208
BstXI CCAn_nnnn'nTGG
Cuts at: 3457 3457
Size: 6208
BstYI r'GATC_y
Cuts at: 1972 2417 2724 2867 3538 3905 4290 4307 5759
Size: 445 307 143 671 367 385 17 1452
Cuts at: 5759 5845 5857 6203 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 671 445 385 367 346 307
143 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2649 2967 2977 3369 513
Size: 552 1584 318 10 392 3352
Fragments arranged by size:
3352 1584 552 392 318 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3244 487
Size: 683 56 2018 3451
Fragments arranged by size:
3451 2018 683 56
BtsI GCAGTG_nn'
Cuts at: 3215 3286 3724 4518 4538 3215
Size: 71 438 794 20 4885
Fragments arranged by size:
4885 794 438 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5215 809
Size: 507 3899 1802
Fragments arranged by size:
3899 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2130 2598 2751
Size: 54 1425 50 275 18 300 468 153
Cuts at: 2751 2815 3040 3091 3156 3655 4544 6173 8
Size: 64 225 51 65 499 889 1629 43
Fragments arranged by size:
1629 1425 889 499 468 300 275 225
153 65 64 54 51 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2815 3655 1537
Size: 275 1003 840 4090
Fragments arranged by size:
4090 1003 840 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2906 3440 4138 1353
Size: 1553 534 698 3423
Fragments arranged by size:
3423 1553 698 534
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2161 2462 2711 2813 2957 2963 3347
Size: 393 529 301 249 102 144 6 384
Cuts at: 3347 4271 4868 5655 5923 1239
Size: 924 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 529 393 384 301
268 249 144 102 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2693 2693
Size: 6208
EcoO109I rG'GnC_Cy
Cuts at: 2154 2463 2645 3022 3058 3148 2154
Size: 309 182 377 36 90 5214
Fragments arranged by size:
5214 377 309 182 90 36
EcoRI G'AATT_C
Cuts at: 2063 3517 3895 2063
Size: 1454 378 4376
Fragments arranged by size:
4376 1454 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2402 2402
Size: 6208
FspI TGC'GCA
Cuts at: 4714 4714
Size: 6208
HaeII r_GCGC'y
Cuts at: 1678 5355 1678
Size: 3677 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2831 2863 3365 3397 4849
Size: 32 135 32 1259 32 502 32 1452
Cuts at: 4849 4881 4923 4955 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 1259 502 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3226 3552 3798 669
Size: 988 281 1288 326 246 3079
Fragments arranged by size:
3079 1288 988 326 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KpnI G_GTAC'C
Cuts at: 2044 3533 2044
Size: 1489 4719
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 3188 5322 5506 1305
Size: 1260 623 2134 184 2007
Fragments arranged by size:
2134 2007 1260 623 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2132 2600 3093 3158 10
Size: 54 1425 343 300 468 493 65 3060
Fragments arranged by size:
3060 1425 493 468 343 300 65 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2196 3485 4166 4525 4684 518
Size: 648 1030 1289 681 359 159 2042
Fragments arranged by size:
2042 1289 1030 681 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2762 2791 2805 2814 2929 3133 3624 4301
Size: 1156 29 14 9 115 204 491 677
Cuts at: 4301 5449 5694 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1156 1148 677 491 245 204 115
29 14 9
NaeI GCC'GGC
Cuts at: 2819 2826 2819
Size: 7 6201
NcoI C'CATG_G
Cuts at: 513 1065 3369 513
Size: 552 2304 3352
Fragments arranged by size:
3352 2304 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NgoMIV G'CCGG_C
Cuts at: 2817 2824 2817
Size: 7 6201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 2524 3224 3566 5111 1132
Size: 97 1295 700 342 1545 2229
Fragments arranged by size:
2229 1545 1295 700 342 97
PasI CC'CwG_GG
Cuts at: 1348 2110 1348
Size: 762 5446
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6208
PfoI T'CCnGG_A
Cuts at: 2389 2389
Size: 6208
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3107 3139 4277 4309 5790 5822 3107
Size: 32 1138 32 1481 32 3493
Fragments arranged by size:
3493 1481 1138 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2645 3058 2645
Size: 413 5795
PshAI GACnn'nnGTC
Cuts at: 1613 2643 1613
Size: 1030 5178
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 2462 3147 2462
Size: 685 5523
PstI C_TGCA'G
Cuts at: 830 3206 3506 3560 830
Size: 2376 300 54 3478
Fragments arranged by size:
3478 2376 300 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2791 2805 2929 2791
Size: 14 124 6070
Fragments arranged by size:
6070 124 14
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SapI GCTCTTCn'nnn_
Cuts at: 2906 2906
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SfcI C'TryA_G
Cuts at: 826 1045 2355 3202 3502 3556 4691 5372 5563
Size: 219 1310 847 300 54 1135 681 191
Cuts at: 5563 826
Size: 1471
Fragments arranged by size:
1471 1310 1135 847 681 300 219 191
54
SfiI GGCCn_nnn'nGGCC
Cuts at: 3155 3155
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2461 3535 1872
Size: 589 1074 4545
Fragments arranged by size:
4545 1074 589
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3191 3269 4312 5213 5475 820
Size: 197 926 1248 78 1043 901 262 1553
Fragments arranged by size:
1553 1248 1043 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SrfI GCCC'GGGC
Cuts at: 2461 2461
Size: 6208
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2259 2859 2948 1517
Size: 742 600 89 4777
Fragments arranged by size:
4777 742 600 89
StyI C'CwwG_G
Cuts at: 513 1065 2145 2478 3369 3647 513
Size: 552 1080 333 891 278 3074
Fragments arranged by size:
3074 1080 891 552 333 278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2072 2655 3406 4235 4252 4405 4590
Size: 1255 492 583 751 829 17 153 185
Cuts at: 4590 5035 325
Size: 445 1498
Fragments arranged by size:
1498 1255 829 751 583 492 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2517 2532 4454
Size: 274 80 33 51 1443 540 15 1922
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1922 1712 1443 540 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6168 6200 6168
Size: 32 6176
Tth111I GACn'n_nGTC
Cuts at: 1316 2542 3482 1316
Size: 1226 940 4042
Fragments arranged by size:
4042 1226 940
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2125
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2459 3533 1870
Size: 589 1074 4545
Fragments arranged by size:
4545 1074 589
XmnI GAAnn'nnTTC
Cuts at: 2174 2900 4337 2174
Size: 726 1437 4045
Fragments arranged by size:
4045 1437 726
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NarI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI
SfoI SwaI