Restriction map for
pFN21AE1944
(Circular) (Six-base) MAPSORT of: pFN21AE1944.seq Check: 4877 from: 1 to: 6205
pFN21AE1944 6205 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4015 278
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3548 1937
Size: 1611 4594
Acc65I G'GTAC_C
Cuts at: 3526 3526
Size: 6205
AclI AA'CG_TT
Cuts at: 1563 4332 4705 1563
Size: 2769 373 3063
Fragments arranged by size:
3063 2769 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2969 3125 3491 4268 5652 5920 1632
Size: 1337 156 366 777 1384 268 1917
Fragments arranged by size:
1917 1384 1337 777 366 268 156
AfeI AGC'GCT
Cuts at: 2244 3225 2244
Size: 981 5224
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6008
AflIII A'CryG_T
Cuts at: 1203 5104 6081 1203
Size: 3901 977 1327
Fragments arranged by size:
3901 1327 977
AgeI A'CCGG_T
Cuts at: 5036 5036
Size: 6205
AhdI GACnn_n'nnGTC
Cuts at: 4934 4934
Size: 6205
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6205
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3341 1184
Size: 2157 4048
AlwNI CAG_nnn'CTG
Cuts at: 2749 3271 3430 3948 5520 2749
Size: 522 159 518 1572 3434
Fragments arranged by size:
3434 1572 522 518 159
ApaI G_GGCC'C
Cuts at: 2400 2541 2684 2400
Size: 141 143 5921
Fragments arranged by size:
5921 143 141
ApaLI G'TGCA_C
Cuts at: 3316 4262 5418 3316
Size: 946 1156 4103
Fragments arranged by size:
4103 1156 946
ApoI r'AATT_y
Cuts at: 1493 3514 3740 3892 6001 1493
Size: 2021 226 152 2109 1697
Fragments arranged by size:
2109 2021 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4759 160
Size: 872 3727 1606
Fragments arranged by size:
3727 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
AvaI C'yCGr_G
Cuts at: 1870 1943 2033 2066 2293 2510 2684 3530 1870
Size: 73 90 33 227 217 174 846 4545
Fragments arranged by size:
4545 846 227 217 174 90 73 33
BamHI G'GATC_C
Cuts at: 2380 3535 3902 2380
Size: 1155 367 4683
Fragments arranged by size:
4683 1155 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2319 3206 3284 3526 4981
Size: 325 217 627 532 887 78 242 1455
Cuts at: 4981 618
Size: 1842
Fragments arranged by size:
1842 1455 887 627 532 325 242 217
78
BanII G_rGCy'C
Cuts at: 1391 2067 2286 2400 2541 2684 2741 3524 1391
Size: 676 219 114 141 143 57 783 4072
Fragments arranged by size:
4072 783 676 219 143 141 114 57
BbeI G_GCGC'C
Cuts at: 2323 2323
Size: 6205
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2613 928
Size: 971 714 4520
Fragments arranged by size:
4520 971 714
BbvCI CC'TCA_GC
Cuts at: 2691 2742 2691
Size: 51 6154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4396 4430 1903
Size: 34 2459 34 3678
Fragments arranged by size:
3678 2459 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4097 5307 4097
Size: 1210 4995
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6205
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2963 4816 136
Size: 107 122 71 631 605 1291 1853 1525
Fragments arranged by size:
1853 1525 1291 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3474 6200 3474
Size: 2726 3479
BlpI GC'TnA_GC
Cuts at: 2735 3622 2735
Size: 887 5318
Bme1580I G_kGCm'C
Cuts at: 1790 2400 2541 2684 2941 2968 3034 3209 3289
Size: 610 141 143 257 27 66 175 80
Cuts at: 3289 3320 4266 5422 1790
Size: 31 946 1156 2573
Fragments arranged by size:
2573 1156 946 610 257 175 143 141
80 66 31 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6205
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3936 4894 442
Size: 416 53 83 399 24 2519 958 1753
Fragments arranged by size:
2519 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6205
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2536 2536
Size: 6205
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2393 2511 3077 3260 4865 1239
Size: 1154 118 566 183 1605 2579
Fragments arranged by size:
2579 1605 1154 566 183 118
Bpu10I CC'TnA_GC
Cuts at: 2691 2742 2691
Size: 51 6154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4330 5195 5493 4330
Size: 865 298 5042
Fragments arranged by size:
5042 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3353 4868 882
Size: 617 1854 1515 2219
Fragments arranged by size:
2219 1854 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 2725 3996 493
Size: 711 1521 1271 2702
Fragments arranged by size:
2702 1521 1271 711
BsaBI GATnn'nnATC
Cuts at: 6199 6199
Size: 6205
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2320 3419 4012 4394
Size: 53 83 186 936 787 1099 593 382
Cuts at: 4394 5029 275
Size: 635 1451
Fragments arranged by size:
1451 1099 936 787 635 593 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2081 2102 2165 4638 5036 5310 5457 5868 2081
Size: 21 63 2473 398 274 147 411 2418
Fragments arranged by size:
2473 2418 411 398 274 147 63 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2509 2539 2557
Size: 30 165 30 1047 30 29 30 18
Cuts at: 2557 2587 1178
Size: 30 4796
Fragments arranged by size:
4796 1047 165 30 30 30 30 30
29 18
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2196 2199 2471 2635 2638 1186
Size: 1010 3 272 164 3 4753
Fragments arranged by size:
4753 1010 272 164 3 3
BseYI C'CCAG_C
Cuts at: 2225 2556 2674 2818 5408 5904 2225
Size: 331 118 144 2590 496 2526
Fragments arranged by size:
2590 2526 496 331 144 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2548 3212 3218 3311 1163
Size: 573 812 664 6 93 4057
Fragments arranged by size:
4057 812 664 573 93 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2405 3655 4416 5444 1540
Size: 275 186 404 1250 761 1028 2301
Fragments arranged by size:
2301 1250 1028 761 404 275 186
BsiHKAI G_wGCw'C
Cuts at: 3320 4266 4351 5422 3320
Size: 946 85 1071 4103
Fragments arranged by size:
4103 1071 946 85
BsmI GAATG_Cn'
Cuts at: 3716 3809 3716
Size: 93 6112
BsmBI CGTCTCn'nnnn_
Cuts at: 3410 3410
Size: 6205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2286 2400 2541 2684 2741 2941
Size: 399 277 219 114 141 143 57 200
Cuts at: 2941 2968 3034 3209 3289 3320 3524 4266 4351
Size: 27 66 175 80 31 204 742 85
Cuts at: 4351 5422 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 277 219 204 200
175 143 141 114 85 80 66 57
31 27
BspEI T'CCGG_A
Cuts at: 2081 2102 2165 5868 2081
Size: 21 63 3703 2418
Fragments arranged by size:
3703 2418 63 21
BspHI T'CATG_A
Cuts at: 2809 4094 5824 2809
Size: 1285 1730 3190
Fragments arranged by size:
3190 1730 1285
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3560 844
Size: 2716 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2326 2623 3253 4092 1394
Size: 429 503 297 630 839 3507
Fragments arranged by size:
3507 839 630 503 429 297
BsrDI GCAATG_nn'
Cuts at: 65 1248 3129 4700 4874 65
Size: 1183 1881 1571 174 1396
Fragments arranged by size:
1881 1571 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2270 2404 2976 4849 5036 1700
Size: 110 460 134 572 1873 187 2869
Fragments arranged by size:
2869 1873 572 460 187 134 110
BsrGI T'GTAC_A
Cuts at: 96 2908 96
Size: 2812 3393
BssHII G'CGCG_C
Cuts at: 1925 2266 1925
Size: 341 5864
BssSI C'ACGA_G
Cuts at: 4265 5277 4265
Size: 1012 5193
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6205
BstBI TT'CG_AA
Cuts at: 5009 5009
Size: 6205
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6205
BstYI r'GATC_y
Cuts at: 1972 2380 3474 3535 3902 4287 4304 5756 5842
Size: 408 1094 61 367 385 17 1452 86
Cuts at: 5842 5854 6200 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1094 408 385 367 346 86
61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2309 2415 513
Size: 552 1244 106 4303
Fragments arranged by size:
4303 1244 552 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5466
Fragments arranged by size:
5466 683 56
BtsI GCAGTG_nn'
Cuts at: 2808 3721 4515 4535 2808
Size: 913 794 20 4478
Fragments arranged by size:
4478 913 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6205
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6170
DraI TTT'AAA
Cuts at: 3509 3509
Size: 6205
DraIII CAC_nnn'GTG
Cuts at: 3372 3372
Size: 6205
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2789 5212 809
Size: 507 1473 2423 1802
Fragments arranged by size:
2423 1802 1473 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2237 2402 2406
Size: 54 1425 50 275 18 407 165 4
Cuts at: 2406 3053 3340 3652 4541 6170 8
Size: 647 287 312 889 1629 43
Fragments arranged by size:
1629 1425 889 647 407 312 287 275
165 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2402 3652 1537
Size: 275 590 1250 4090
Fragments arranged by size:
4090 1250 590 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4135 1353
Size: 2782 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2380 2468 4792 5166 5312 387
Size: 1064 138 791 88 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 791 374 146 138 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2393 2511 2969 3077 3125 3260 3491
Size: 393 761 118 458 108 48 135 231
Cuts at: 3491 4268 4865 5652 5920 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 787 777 761 597 458 393 268
231 135 118 108 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3275 3434 3275
Size: 159 6046
EcoO109I rG'GnC_Cy
Cuts at: 2093 2189 2397 2538 2579 2680 2681 2695 2815
Size: 96 208 141 41 101 1 14 120
Cuts at: 2815 2860 3256 3427 2093
Size: 45 396 171 4871
Fragments arranged by size:
4871 396 208 171 141 120 101 96
45 41 14 1
EcoRI G'AATT_C
Cuts at: 3514 3892 3514
Size: 378 5827
FseI GG_CCGG'CC
Cuts at: 2408 2408
Size: 6205
FspI TGC'GCA
Cuts at: 4711 4711
Size: 6205
HaeII r_GCGC'y
Cuts at: 1678 2112 2246 2323 2735 3016 3227 5352 1678
Size: 434 134 77 412 281 211 2125 2531
Fragments arranged by size:
2531 2125 434 412 281 211 134 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4846 4878 4920 4952 1373
Size: 32 135 32 3274 32 42 32 2626
Fragments arranged by size:
3274 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3549 3795 669
Size: 988 281 1611 246 3079
Fragments arranged by size:
3079 1611 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6205
HpaI GTT'AAC
Cuts at: 3795 3795
Size: 6205
KasI G'GCGC_C
Cuts at: 2319 2319
Size: 6205
KpnI G_GTAC'C
Cuts at: 3530 3530
Size: 6205
MfeI C'AATT_G
Cuts at: 3804 3804
Size: 6205
MluI A'CGCG_T
Cuts at: 6081 6081
Size: 6205
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2406 5319 5503 1305
Size: 1101 2913 184 2007
Fragments arranged by size:
2913 2007 1101 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2239 10
Size: 54 1425 343 407 3976
Fragments arranged by size:
3976 1425 407 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 3194 4163 4522 4681 518
Size: 648 978 1050 969 359 159 2042
Fragments arranged by size:
2042 1050 978 969 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2225 2311 2417 2749 2822 3036 3097 3338
Size: 619 86 106 332 73 214 61 241
Cuts at: 3338 3621 4298 5446 5691 1606
Size: 283 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 619 332 283 245 241
214 106 86 73 61
NaeI GCC'GGC
Cuts at: 2272 2406 2272
Size: 134 6071
NarI GG'CG_CC
Cuts at: 2320 2320
Size: 6205
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5653
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6205
NgoMIV G'CCGG_C
Cuts at: 2270 2404 2270
Size: 134 6071
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6205
NotI GC'GGCC_GC
Cuts at: 3652 3652
Size: 6205
NspI r_CATG'y
Cuts at: 1132 1229 2425 3307 3563 5108 1132
Size: 97 1196 882 256 1545 2229
Fragments arranged by size:
2229 1545 1196 882 256 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6205
PciI A'CATG_T
Cuts at: 5104 5104
Size: 6205
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3022 1108
Size: 1914 4291
PfoI T'CCnGG_A
Cuts at: 2575 2862 2575
Size: 287 5918
PmeI GTTT'AAAC
Cuts at: 3509 3509
Size: 6205
PmlI CAC'GTG
Cuts at: 2725 2725
Size: 6205
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4274 4306 5787 5819 4274
Size: 32 1481 32 4660
Fragments arranged by size:
4660 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2189 2579 2695 2860 3427 2189
Size: 390 116 165 567 4967
Fragments arranged by size:
4967 567 390 165 116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6205
PsiI TTA'TAA
Cuts at: 3775 3775
Size: 6205
PspOMI G'GGCC_C
Cuts at: 2396 2537 2680 2396
Size: 141 143 5921
Fragments arranged by size:
5921 143 141
PstI C_TGCA'G
Cuts at: 830 3557 830
Size: 2727 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6205
PvuII CAG'CTG
Cuts at: 2749 3097 3338 2749
Size: 348 241 5616
Fragments arranged by size:
5616 348 241
SacII CC_GC'GG
Cuts at: 2312 2418 2312
Size: 106 6099
SalI G'TCGA_C
Cuts at: 1936 3547 1936
Size: 1611 4594
SanDI GG'GwC_CC
Cuts at: 2579 2579
Size: 6205
SbfI CC_TGCA'GG
Cuts at: 3557 3557
Size: 6205
ScaI AGT'ACT
Cuts at: 4453 6165 4453
Size: 1712 4493
SfcI C'TryA_G
Cuts at: 826 1045 2969 3553 4688 5369 5560 826
Size: 219 1924 584 1135 681 191 1471
Fragments arranged by size:
1924 1471 1135 681 584 219 191
SfoI GGC'GCC
Cuts at: 2321 2321
Size: 6205
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6205
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6205
SmaI CCC'GGG
Cuts at: 1872 2035 2068 2295 3532 1872
Size: 163 33 227 1237 4545
Fragments arranged by size:
4545 1237 227 163 33
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4309 5210 5472 820
Size: 197 926 2366 901 262 1553
Fragments arranged by size:
2366 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3996 493
Size: 3503 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6205
SphI G_CATG'C
Cuts at: 1132 3307 3563 1132
Size: 2175 256 3774
Fragments arranged by size:
3774 2175 256
SspI AAT'ATT
Cuts at: 5 52 4129 5
Size: 47 4077 2081
Fragments arranged by size:
4077 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6205
StyI C'CwwG_G
Cuts at: 513 1065 3644 513
Size: 552 2579 3074
Fragments arranged by size:
3074 2579 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2217 2582 4232 4249 4402 4587 5032
Size: 1255 637 365 1650 17 153 185 445
Cuts at: 5032 325
Size: 1498
Fragments arranged by size:
1650 1498 1255 637 445 365 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2908 4451 6163
Size: 274 80 33 51 1443 931 1543 1712
Cuts at: 6163 96
Size: 138
Fragments arranged by size:
1712 1543 1443 931 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2509 2541 2557 2589 6165 6197 2509
Size: 32 16 32 3576 32 2517
Fragments arranged by size:
3576 2517 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6205
XbaI T'CTAG_A
Cuts at: 3541 3541
Size: 6205
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2471 2471
Size: 6205
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6205
XmaI C'CCGG_G
Cuts at: 1870 2033 2066 2293 3530 1870
Size: 163 33 227 1237 4545
Fragments arranged by size:
4545 1237 227 163 33
XmnI GAAnn'nnTTC
Cuts at: 4334 4334
Size: 6205
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4013 276
Size: 53 83 186 936 2479 2468
Fragments arranged by size:
2479 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstXI
BstZ17I Bsu36I EcoICRI EcoRV FalI FspAI NruI NsiI
PacI PspXI PsrI RsrII SacI SapI SexAI SfiI
SrfI SwaI