Restriction map for
pFN21AE1943
(Circular) (Six-base) MAPSORT of: pFN21AE1943.seq Check: 5644 from: 1 to: 6199
pFN21AE1943 6199 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4009 278
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2072 3542 1937
Size: 135 1470 4594
Fragments arranged by size:
4594 1470 135
Acc65I G'GTAC_C
Cuts at: 3520 3520
Size: 6199
AclI AA'CG_TT
Cuts at: 1563 4326 4699 1563
Size: 2763 373 3063
Fragments arranged by size:
3063 2763 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4262 5646 5914 1632
Size: 2630 1384 268 1917
Fragments arranged by size:
2630 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6002
AflIII A'CryG_T
Cuts at: 1203 2145 5098 6075 1203
Size: 942 2953 977 1327
Fragments arranged by size:
2953 1327 977 942
AgeI A'CCGG_T
Cuts at: 5030 5030
Size: 6199
AhdI GACnn_n'nnGTC
Cuts at: 4928 4928
Size: 6199
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6199
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2959 1184
Size: 1775 4424
AlwNI CAG_nnn'CTG
Cuts at: 3042 3942 5514 3042
Size: 900 1572 3727
Fragments arranged by size:
3727 1572 900
ApaLI G'TGCA_C
Cuts at: 4256 5412 4256
Size: 1156 5043
ApoI r'AATT_y
Cuts at: 1493 2897 3508 3734 3886 5995 1493
Size: 1404 611 226 152 2109 1697
Fragments arranged by size:
2109 1697 1404 611 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2468 4753 160
Size: 872 1436 2285 1606
Fragments arranged by size:
2285 1606 1436 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
AvaI C'yCGr_G
Cuts at: 1870 1943 3524 1870
Size: 73 1581 4545
Fragments arranged by size:
4545 1581 73
BamHI G'GATC_C
Cuts at: 3529 3896 3529
Size: 367 5832
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2927 3520 4975 618
Size: 325 217 627 1140 593 1455 1842
Fragments arranged by size:
1842 1455 1140 627 593 325 217
BanII G_rGCy'C
Cuts at: 1391 3518 1391
Size: 2127 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3322 928
Size: 971 1423 3805
Fragments arranged by size:
3805 1423 971
BbvCI CC'TCA_GC
Cuts at: 2922 3069 2922
Size: 147 6052
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3008 3042 4390 4424 1903
Size: 34 1071 34 1348 34 3678
Fragments arranged by size:
3678 1348 1071 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4091 5301 4091
Size: 1210 4989
BclI T'GATC_A
Cuts at: 1548 2377 1548
Size: 829 5370
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4810 136
Size: 107 122 71 631 605 3138 1525
Fragments arranged by size:
3138 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3434 6194 3434
Size: 2760 3439
BlpI GC'TnA_GC
Cuts at: 3616 3616
Size: 6199
Bme1580I G_kGCm'C
Cuts at: 1790 2972 4260 5416 1790
Size: 1182 1288 1156 2573
Fragments arranged by size:
2573 1288 1182 1156
BmgBI CAC'GTC
Cuts at: 1318 2146 1318
Size: 828 5371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3930 4888 442
Size: 416 53 83 399 24 2513 958 1753
Fragments arranged by size:
2513 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4859 1239
Size: 3620 2579
Bpu10I CC'TnA_GC
Cuts at: 2922 3069 2922
Size: 147 6052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4324 5189 5487 4324
Size: 865 298 5036
Fragments arranged by size:
5036 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3014 4862 882
Size: 617 1515 1848 2219
Fragments arranged by size:
2219 1848 1515 617
BsaAI yAC'GTr
Cuts at: 493 1204 3990 493
Size: 711 2786 2702
Fragments arranged by size:
2786 2702 711
BsaBI GATnn'nnATC
Cuts at: 6193 6193
Size: 6199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4006 4388 5023 275
Size: 53 83 186 936 2473 382 635 1451
Fragments arranged by size:
2473 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4632 5030 5304 5451 5862 4632
Size: 398 274 147 411 4969
Fragments arranged by size:
4969 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5974
Fragments arranged by size:
5974 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6199
BseYI C'CCAG_C
Cuts at: 2034 5402 5898 2034
Size: 3368 496 2335
Fragments arranged by size:
3368 2335 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3429 1163
Size: 573 1693 3933
Fragments arranged by size:
3933 1693 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 2167 3649 4410 5438 1540
Size: 275 186 29 137 1482 761 1028 2301
Fragments arranged by size:
2301 1482 1028 761 275 186 137 29
BsiHKAI G_wGCw'C
Cuts at: 4260 4345 5416 4260
Size: 85 1071 5043
Fragments arranged by size:
5043 1071 85
BsmI GAATG_Cn'
Cuts at: 3710 3803 3710
Size: 93 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2972 3518 4260 4345 5416 1391
Size: 399 1182 546 742 85 1071 2174
Fragments arranged by size:
2174 1182 1071 742 546 399 85
BspEI T'CCGG_A
Cuts at: 5862 5862
Size: 6199
BspHI T'CATG_A
Cuts at: 2991 4088 5818 2991
Size: 1097 1730 3372
Fragments arranged by size:
3372 1730 1097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3554 844
Size: 2710 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2023 4086 1394
Size: 429 200 2063 3507
Fragments arranged by size:
3507 2063 429 200
BsrDI GCAATG_nn'
Cuts at: 65 1248 2295 2577 2931 2941 3024 4694 4868
Size: 1183 1047 282 354 10 83 1670 174
Cuts at: 4868 65
Size: 1396
Fragments arranged by size:
1670 1396 1183 1047 354 282 174 83
10
BsrFI r'CCGG_y
Cuts at: 1700 1810 4843 5030 1700
Size: 110 3033 187 2869
Fragments arranged by size:
3033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2215 96
Size: 2119 4080
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6199
BssSI C'ACGA_G
Cuts at: 4259 5271 4259
Size: 1012 5187
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2399 794
Size: 1605 4594
BstBI TT'CG_AA
Cuts at: 5003 5003
Size: 6199
BstEII G'GTnAC_C
Cuts at: 1184 2720 1184
Size: 1536 4663
BstYI r'GATC_y
Cuts at: 1972 2094 3039 3434 3529 3896 4281 4298 5750
Size: 122 945 395 95 367 385 17 1452
Cuts at: 5750 5836 5848 6194 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 945 395 385 367 346 122
95 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5647
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5460
Fragments arranged by size:
5460 683 56
BtsI GCAGTG_nn'
Cuts at: 3412 3460 3715 4509 4529 3412
Size: 48 255 794 20 5082
Fragments arranged by size:
5082 794 255 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6199
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6164
DraI TTT'AAA
Cuts at: 2652 3503 2652
Size: 851 5348
DraIII CAC_nnn'GTG
Cuts at: 3287 3287
Size: 6199
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5206 809
Size: 507 3890 1802
Fragments arranged by size:
3890 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3646 4535 6164
Size: 54 1425 50 275 18 1816 889 1629
Cuts at: 6164 8
Size: 43
Fragments arranged by size:
1816 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3646 1537
Size: 275 1834 4090
Fragments arranged by size:
4090 1834 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4129 1353
Size: 2776 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4786 5160 5306 387
Size: 1064 138 3197 374 146 1280
Fragments arranged by size:
3197 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4262 4859 5646 5914 1239
Size: 393 2630 597 787 268 1524
Fragments arranged by size:
2630 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2131 2131
Size: 6199
EcoRI G'AATT_C
Cuts at: 2897 3508 3886 2897
Size: 611 378 5210
Fragments arranged by size:
5210 611 378
EcoRV GAT'ATC
Cuts at: 2908 2908
Size: 6199
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2972 2972
Size: 6199
FspI TGC'GCA
Cuts at: 4705 4705
Size: 6199
HaeII r_GCGC'y
Cuts at: 1678 2065 5346 1678
Size: 387 3281 2531
Fragments arranged by size:
3281 2531 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2012 2044 3052 3084 4840
Size: 32 135 32 440 32 1008 32 1756
Cuts at: 4840 4872 4914 4946 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1756 1008 440 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2073 3543 3789 669
Size: 988 281 135 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246 135
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6199
HpaI GTT'AAC
Cuts at: 3789 3789
Size: 6199
KpnI G_GTAC'C
Cuts at: 3524 3524
Size: 6199
MfeI C'AATT_G
Cuts at: 3798 3798
Size: 6199
MluI A'CGCG_T
Cuts at: 6075 6075
Size: 6199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2392 5313 5497 1305
Size: 1087 2921 184 2007
Fragments arranged by size:
2921 2007 1087 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4377
Fragments arranged by size:
4377 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 4157 4516 4675 518
Size: 648 1173 1818 359 159 2042
Fragments arranged by size:
2042 1818 1173 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3615 4292 5440 5685 1606
Size: 2009 677 1148 245 2120
Fragments arranged by size:
2120 2009 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5647
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6199
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6199
NotI GC'GGCC_GC
Cuts at: 3646 3646
Size: 6199
NspI r_CATG'y
Cuts at: 1132 1229 3557 5102 1132
Size: 97 2328 1545 2229
Fragments arranged by size:
2328 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6199
PciI A'CATG_T
Cuts at: 5098 5098
Size: 6199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3030 3105 1108
Size: 1922 75 4202
Fragments arranged by size:
4202 1922 75
PmeI GTTT'AAAC
Cuts at: 3503 3503
Size: 6199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4268 4300 5781 5813 4268
Size: 32 1481 32 4654
Fragments arranged by size:
4654 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2131 2131
Size: 6199
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6199
PsiI TTA'TAA
Cuts at: 3769 3769
Size: 6199
PstI C_TGCA'G
Cuts at: 830 3047 3551 830
Size: 2217 504 3478
Fragments arranged by size:
3478 2217 504
PvuI CG_AT'CG
Cuts at: 2001 2167 2001
Size: 166 6033
SalI G'TCGA_C
Cuts at: 1936 2071 3541 1936
Size: 135 1470 4594
Fragments arranged by size:
4594 1470 135
SanDI GG'GwC_CC
Cuts at: 2131 2131
Size: 6199
SbfI CC_TGCA'GG
Cuts at: 3551 3551
Size: 6199
ScaI AGT'ACT
Cuts at: 4447 6159 4447
Size: 1712 4487
SfcI C'TryA_G
Cuts at: 826 1045 2188 3043 3547 4682 5363 5554 826
Size: 219 1143 855 504 1135 681 191 1471
Fragments arranged by size:
1471 1143 1135 855 681 504 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6199
SmaI CCC'GGG
Cuts at: 1872 3526 1872
Size: 1654 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4303 5204 5466 820
Size: 197 926 2360 901 262 1553
Fragments arranged by size:
2360 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3990 493
Size: 3497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6199
SphI G_CATG'C
Cuts at: 1132 3557 1132
Size: 2425 3774
SspI AAT'ATT
Cuts at: 5 52 2466 2756 4123 5
Size: 47 2414 290 1367 2081
Fragments arranged by size:
2414 2081 1367 290 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6199
StyI C'CwwG_G
Cuts at: 513 1065 2956 3638 513
Size: 552 1891 682 3074
Fragments arranged by size:
3074 1891 682 552
SwaI ATTT'AAAT
Cuts at: 2652 2652
Size: 6199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2685 4226 4243 4396 4581 5026 325
Size: 1255 1105 1541 17 153 185 445 1498
Fragments arranged by size:
1541 1498 1255 1105 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2215 2938 4445
Size: 274 80 33 51 1443 238 723 1507
Cuts at: 4445 6157 96
Size: 1712 138
Fragments arranged by size:
1712 1507 1443 723 274 238 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3439 3471 6159 6191 3439
Size: 32 2688 32 3447
Fragments arranged by size:
3447 2688 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6199
XbaI T'CTAG_A
Cuts at: 3535 3535
Size: 6199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3106 3106
Size: 6199
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6199
XmaI C'CCGG_G
Cuts at: 1870 3524 1870
Size: 1654 4545
XmnI GAAnn'nnTTC
Cuts at: 4328 4328
Size: 6199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4007 276
Size: 53 83 186 936 2473 2468
Fragments arranged by size:
2473 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SapI SexAI SfiI SfoI SrfI