Restriction map for
pFN21AE1938
(Circular) (Six-base) MAPSORT of: pFN21AE1938.seq Check: 8410 from: 1 to: 6145
pFN21AE1938 6145 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3488 1937
Size: 1551 4594
Acc65I G'GTAC_C
Cuts at: 3466 3466
Size: 6145
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2976 3019 4208 5592 5860 1632
Size: 1344 43 1189 1384 268 1917
Fragments arranged by size:
1917 1384 1344 1189 268 43
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 5044 6021 1203
Size: 3841 977 1327
Fragments arranged by size:
3841 1327 977
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 2754 1166
Size: 1588 4557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AlwNI CAG_nnn'CTG
Cuts at: 2705 2728 2999 3042 3374 3888 5460 2705
Size: 23 271 43 332 514 1572 3390
Fragments arranged by size:
3390 1572 514 332 271 43 23
ApaI G_GGCC'C
Cuts at: 2359 2616 2359
Size: 257 5888
ApaLI G'TGCA_C
Cuts at: 2969 3069 4202 5358 2969
Size: 100 1133 1156 3756
Fragments arranged by size:
3756 1156 1133 100
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 2379 3171 3470 1870
Size: 73 436 792 299 4545
Fragments arranged by size:
4545 792 436 299 73
BamHI G'GATC_C
Cuts at: 3414 3475 3842 3414
Size: 61 367 5717
Fragments arranged by size:
5717 367 61
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3466 4921 618
Size: 325 217 627 1679 1455 1842
Fragments arranged by size:
1842 1679 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2359 2442 2616 3464 1391
Size: 968 83 174 848 4072
Fragments arranged by size:
4072 968 848 174 83
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2481 928
Size: 971 582 4592
Fragments arranged by size:
4592 971 582
BbvCI CC'TCA_GC
Cuts at: 2663 2663
Size: 6145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4336 4370 1903
Size: 34 2399 34 3678
Fragments arranged by size:
3678 2399 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BfrBI ATG'CAT
Cuts at: 2534 2534
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2864 4756
Size: 107 122 71 631 605 337 855 1892
Cuts at: 4756 136
Size: 1525
Fragments arranged by size:
1892 1525 855 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 3333 6140 3333
Size: 2807 3338
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 2047 2329 2359 2588 2616 2793 2973 3073
Size: 257 282 30 229 28 177 180 100
Cuts at: 3073 3365 4206 5362 1790
Size: 292 841 1156 2573
Fragments arranged by size:
2573 1156 841 292 282 257 229 180
177 100 30 28
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2610 3876 4834
Size: 416 53 83 399 24 1193 1266 958
Cuts at: 4834 442
Size: 1753
Fragments arranged by size:
1753 1266 1193 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 3355 2212
Size: 1143 5002
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2114 2351 2727 4805 1239
Size: 875 237 376 2078 2579
Fragments arranged by size:
2579 2078 875 376 237
Bpu10I CC'TnA_GC
Cuts at: 2121 2663 2121
Size: 542 5603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4808 882
Size: 617 3309 2219
Fragments arranged by size:
3309 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2863 3329 3936 493
Size: 711 1659 466 607 2702
Fragments arranged by size:
2702 1659 711 607 466
BsaBI GATnn'nnATC
Cuts at: 6139 6139
Size: 6145
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2090 3952 4334
Size: 53 83 186 936 479 78 1862 382
Cuts at: 4334 4969 275
Size: 635 1451
Fragments arranged by size:
1862 1451 936 635 479 382 186 83
78 53
BsaWI w'CCGG_w
Cuts at: 3411 4578 4976 5250 5397 5808 3411
Size: 1167 398 274 147 411 3748
Fragments arranged by size:
3748 1167 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2785 2815 3237 3267 1178
Size: 30 165 30 1382 30 422 30 4056
Fragments arranged by size:
4056 1382 422 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2724 1186
Size: 1538 4607
BseYI C'CCAG_C
Cuts at: 2889 3064 3364 5348 5844 2889
Size: 175 300 1984 496 3190
Fragments arranged by size:
3190 1984 496 300 175
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 2664 2935 1163
Size: 573 741 187 271 4373
Fragments arranged by size:
4373 741 573 271 187
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3595 4356 5384 1540
Size: 275 186 1594 761 1028 2301
Fragments arranged by size:
2301 1594 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2388 2442 2721 2973 3073 4206 4291 5362 2388
Size: 54 279 252 100 1133 85 1071 3171
Fragments arranged by size:
3171 1133 1071 279 252 100 85 54
BsmI GAATG_Cn'
Cuts at: 2576 3656 3749 2576
Size: 1080 93 4972
Fragments arranged by size:
4972 1080 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2138 3047 2138
Size: 909 5236
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2047 2329 2359 2388 2442 2588 2616
Size: 399 257 282 30 29 54 146 28
Cuts at: 2616 2721 2793 2973 3073 3365 3464 4206 4291
Size: 105 72 180 100 292 99 742 85
Cuts at: 4291 5362 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 292 282 257 180
146 105 100 99 85 72 54 30
29 28
BspEI T'CCGG_A
Cuts at: 5808 5808
Size: 6145
BspHI T'CATG_A
Cuts at: 4034 5764 4034
Size: 1730 4415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 2571 3500 844
Size: 1296 431 929 3489
Fragments arranged by size:
3489 1296 929 431
BsrBI CCG'CTC
Cuts at: 1394 1823 2070 4032 1394
Size: 429 247 1962 3507
Fragments arranged by size:
3507 1962 429 247
BsrDI GCAATG_nn'
Cuts at: 65 1248 2868 4640 4814 65
Size: 1183 1620 1772 174 1396
Fragments arranged by size:
1772 1620 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 4789 4976 1700
Size: 110 264 2715 187 2869
Fragments arranged by size:
2869 2715 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 2059 1925
Size: 134 6011
BssSI C'ACGA_G
Cuts at: 2640 4205 5217 2640
Size: 1565 1012 3568
Fragments arranged by size:
3568 1565 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3133 794
Size: 2339 3806
BstBI TT'CG_AA
Cuts at: 4949 4949
Size: 6145
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstXI CCAn_nnnn'nTGG
Cuts at: 2986 2986
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2419 2826 3333 3414 3475 3842 4227 4244
Size: 447 407 507 81 61 367 385 17
Cuts at: 4244 5696 5782 5794 6140 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 507 447 407 385 367 346
86 81 61 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2765 3153 513
Size: 552 939 12 749 388 3505
Fragments arranged by size:
3505 939 749 552 388 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 2329 2827 3134 3375 3661 4455 4475 2329
Size: 498 307 241 286 794 20 3999
Fragments arranged by size:
3999 794 498 307 286 241 20
ClaI AT'CG_AT
Cuts at: 1554 3099 1554
Size: 1545 4600
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3110 3145 529
Size: 35 2546 35 3529
Fragments arranged by size:
3529 2546 35 35
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2865 2922 2938
Size: 54 1425 50 275 18 1035 57 16
Cuts at: 2938 3003 3265 3592 4481 6110 8
Size: 65 262 327 889 1629 43
Fragments arranged by size:
1629 1425 1035 889 327 275 262 65
57 54 50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 3592 1537
Size: 275 1780 4090
Fragments arranged by size:
4090 1780 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4075 1353
Size: 2722 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2819 4732 5106 5252 387
Size: 1064 138 1230 1913 374 146 1280
Fragments arranged by size:
1913 1280 1230 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2440 2440
Size: 6145
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2114 2351 2727 2976 3019 4208 4805
Size: 393 482 237 376 249 43 1189 597
Cuts at: 4805 5592 5860 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1189 787 597 482 393 376 268
249 237 43
EcoNI CCTnn'n_nnAGG
Cuts at: 2333 2333
Size: 6145
EcoO109I rG'GnC_Cy
Cuts at: 2355 2612 2680 2882 2355
Size: 257 68 202 5618
Fragments arranged by size:
5618 257 202 68
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 2129 2411 5292 1678
Size: 451 282 2881 2531
Fragments arranged by size:
2881 2531 451 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2535 2567 3237 3269 4786
Size: 32 135 32 963 32 670 32 1517
Cuts at: 4786 4818 4860 4892 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1517 963 670 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3489 3735 669
Size: 988 281 1551 246 3079
Fragments arranged by size:
3079 1551 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6145
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 3470 3470
Size: 6145
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5259 5443 1305
Size: 3954 184 2007
Fragments arranged by size:
3954 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2867 2924 3005 10
Size: 54 1425 343 1035 57 81 3150
Fragments arranged by size:
3150 1425 1035 343 81 57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2364 2754 2838 3213 4103 4462 4621
Size: 648 1198 390 84 375 890 359 159
Cuts at: 4621 518
Size: 2042
Fragments arranged by size:
2042 1198 890 648 390 375 359 159
84
MspA1I CmG'CkG
Cuts at: 1606 2705 3561 4238 5386 5631 1606
Size: 1099 856 677 1148 245 2120
Fragments arranged by size:
2120 1148 1099 856 677 245
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 6145
NcoI C'CATG_G
Cuts at: 513 1065 2004 2765 513
Size: 552 939 761 3893
Fragments arranged by size:
3893 939 761 552
NdeI CA'TA_TG
Cuts at: 387 2773 387
Size: 2386 3759
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NsiI A_TGCA'T
Cuts at: 2536 2536
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 2570 3430 3503 5048 1132
Size: 97 1341 860 73 1545 2229
Fragments arranged by size:
2229 1545 1341 860 97 73
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6145
PciI A'CATG_T
Cuts at: 5044 5044
Size: 6145
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2605 1108
Size: 1497 4648
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PmlI CAC'GTG
Cuts at: 2863 2863
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2882 2882
Size: 6145
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PspOMI G'GGCC_C
Cuts at: 2355 2612 2355
Size: 257 5888
PstI C_TGCA'G
Cuts at: 830 3497 830
Size: 2667 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2705 2705
Size: 6145
SacI G_AGCT'C
Cuts at: 2442 2442
Size: 6145
SalI G'TCGA_C
Cuts at: 1936 3487 1936
Size: 1551 4594
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SfcI C'TryA_G
Cuts at: 826 1045 2371 2464 3493 4628 5309 5500 826
Size: 219 1326 93 1029 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 1029 681 219 191 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 2381 3173 3472 1872
Size: 509 792 299 4545
Fragments arranged by size:
4545 792 509 299
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 2570 3503 1132
Size: 1438 933 3774
Fragments arranged by size:
3774 1438 933
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2415 2765 3584 513
Size: 552 939 411 350 819 3074
Fragments arranged by size:
3074 939 819 552 411 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 2669 3056 3102 4172 4189 4342
Size: 1255 855 234 387 46 1070 17 153
Cuts at: 4342 4527 4972 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1070 855 445 387 234 185
153 46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3345 4391 6103
Size: 274 80 33 51 1443 1368 1046 1712
Cuts at: 6103 96
Size: 138
Fragments arranged by size:
1712 1443 1368 1046 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2785 2817 6105 6137 2785
Size: 32 3288 32 2793
Fragments arranged by size:
3288 2793 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3211 3481 3211
Size: 270 5875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2212 2212
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 2379 3171 3470 1870
Size: 509 792 299 4545
Fragments arranged by size:
4545 792 509 299
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NarI NruI PacI PfoI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI