Restriction map for
pFN21AE1937
(Circular) (Six-base) MAPSORT of: pFN21AE1937.seq Check: 9774 from: 1 to: 6133
pFN21AE1937 6133 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2430 3229 3476 1937
Size: 493 799 247 4594
Fragments arranged by size:
4594 799 493 247
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2112 4196 5580 5848 1632
Size: 480 2084 1384 268 1917
Fragments arranged by size:
2084 1917 1384 480 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 2235 5032 6009 1203
Size: 1032 2797 977 1327
Fragments arranged by size:
2797 1327 1032 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 2118 1166
Size: 952 5181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AlwNI CAG_nnn'CTG
Cuts at: 2272 2483 3265 3876 5448 2272
Size: 211 782 611 1572 2957
Fragments arranged by size:
2957 1572 782 611 211
ApaLI G'TGCA_C
Cuts at: 4190 5346 4190
Size: 1156 4977
ApoI r'AATT_y
Cuts at: 1493 2288 2302 2365 3037 3202 3442 3668 3820
Size: 795 14 63 672 165 240 226 152
Cuts at: 3820 5929 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 795 672 240 226 165 152
63 14
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 3458 1870
Size: 73 1515 4545
Fragments arranged by size:
4545 1515 73
AvrII C'CTAG_G
Cuts at: 2984 2984
Size: 6133
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3119 3152 3119
Size: 33 6100
BamHI G'GATC_C
Cuts at: 2029 3463 3830 2029
Size: 1434 367 4332
Fragments arranged by size:
4332 1434 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3454 4909 618
Size: 325 217 627 1667 1455 1842
Fragments arranged by size:
1842 1667 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3418 3452 1391
Size: 2027 34 4072
Fragments arranged by size:
4072 2027 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2712 3077 928
Size: 971 813 365 3984
Fragments arranged by size:
3984 971 813 365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4324 4358 1903
Size: 34 2387 34 3678
Fragments arranged by size:
3678 2387 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4744 136
Size: 107 122 71 631 605 3072 1525
Fragments arranged by size:
3072 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2950 6128 2950
Size: 3178 2955
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 4194 5350 1790
Size: 2404 1156 2573
Fragments arranged by size:
2573 2404 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6133
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2964 3075 3864
Size: 416 53 83 399 24 1547 111 789
Cuts at: 3864 4822 442
Size: 958 1753
Fragments arranged by size:
1753 1547 958 789 416 399 111 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3110 1056
Size: 2054 4079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 3296 4793 1239
Size: 861 1196 1497 2579
Fragments arranged by size:
2579 1497 1196 861
Bpu10I CC'TnA_GC
Cuts at: 3352 3352
Size: 6133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2931 3151 4258 5123 5421 2931
Size: 220 1107 865 298 3643
Fragments arranged by size:
3643 1107 865 298 220
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4796 882
Size: 617 3297 2219
Fragments arranged by size:
3297 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3924 493
Size: 711 2720 2702
Fragments arranged by size:
2720 2702 711
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3940 4322 4957 275
Size: 53 83 186 936 2407 382 635 1451
Fragments arranged by size:
2407 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4566 4964 5238 5385 5796 4566
Size: 398 274 147 411 4903
Fragments arranged by size:
4903 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 1178
Size: 30 165 30 624 30 5254
Fragments arranged by size:
5254 624 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2945 1186
Size: 1759 4374
BseYI C'CCAG_C
Cuts at: 3278 5336 5832 3278
Size: 2058 496 3579
Fragments arranged by size:
3579 2058 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2264 2529 2603 1163
Size: 573 528 265 74 4693
Fragments arranged by size:
4693 573 528 265 74
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3418 4194 4279 5350 3418
Size: 776 85 1071 4201
Fragments arranged by size:
4201 1071 776 85
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3418 3452 4194 4279 5350 1391
Size: 399 1628 34 742 85 1071 2174
Fragments arranged by size:
2174 1628 1071 742 399 85 34
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2778 3488 844
Size: 1934 710 3489
Fragments arranged by size:
3489 1934 710
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3237 4628 4802 65
Size: 1183 1989 1391 174 1396
Fragments arranged by size:
1989 1396 1391 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 4193 5205 4193
Size: 1012 5121
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6133
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6133
BstXI CCAn_nnnn'nTGG
Cuts at: 2674 2674
Size: 6133
BstYI r'GATC_y
Cuts at: 1972 2029 2950 3463 3830 4215 4232 5684 5770
Size: 57 921 513 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 921 513 385 367 346 86
57 17 12
BstZ17I GTA'TAC
Cuts at: 2431 2431
Size: 6133
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 4600
Fragments arranged by size:
4600 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3308 487
Size: 683 56 2082 3312
Fragments arranged by size:
3312 2082 683 56
BtsI GCAGTG_nn'
Cuts at: 2166 3649 4443 4463 2166
Size: 1483 794 20 3836
Fragments arranged by size:
3836 1483 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 3437 3437
Size: 6133
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3580 4469 6098
Size: 54 1425 50 275 18 1750 889 1629
Cuts at: 6098 8
Size: 43
Fragments arranged by size:
1750 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4063 1353
Size: 2710 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2653 4720 5094 5240 387
Size: 1064 138 1064 2067 374 146 1280
Fragments arranged by size:
2067 1280 1064 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3416 3416
Size: 6133
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2112 3296 4196 4793 5580 5848
Size: 393 468 12 1184 900 597 787 268
Cuts at: 5848 1239
Size: 1524
Fragments arranged by size:
1524 1184 900 787 597 468 393 268
12
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 5280 1678
Size: 3602 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373
Size: 32 135 32 3202 32 42 32 2626
Fragments arranged by size:
3202 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2166 748
Size: 1418 4715
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2236 2552 5247 5431 1305
Size: 931 316 2695 184 2007
Fragments arranged by size:
2695 2007 931 316 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4311
Fragments arranged by size:
4311 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 2240 2672 3177 4091 4450 4609
Size: 648 952 122 432 505 914 359 159
Cuts at: 4609 518
Size: 2042
Fragments arranged by size:
2042 952 914 648 505 432 359 159
122
MspA1I CmG'CkG
Cuts at: 1606 3549 4226 5374 5619 1606
Size: 1943 677 1148 245 2120
Fragments arranged by size:
2120 1943 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4642
Fragments arranged by size:
4642 939 552
NdeI CA'TA_TG
Cuts at: 387 3178 387
Size: 2791 3342
NheI G'CTAG_C
Cuts at: 1052 3106 1052
Size: 2054 4079
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 2239 3491 5036 1132
Size: 97 1010 1252 1545 2229
Fragments arranged by size:
2229 1545 1252 1010 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 2235 5032 2235
Size: 2797 3336
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6133
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4202 4234 5715 5747 4202
Size: 32 1481 32 4588
Fragments arranged by size:
4588 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2964 1613
Size: 1351 4782
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 2724 3485 830
Size: 1894 761 3478
Fragments arranged by size:
3478 1894 761
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
SacI G_AGCT'C
Cuts at: 3418 3418
Size: 6133
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SexAI A'CCwGG_T
Cuts at: 2439 2439
Size: 6133
SfcI C'TryA_G
Cuts at: 826 1045 2720 3215 3369 3481 4616 5297 5488
Size: 219 1675 495 154 112 1135 681 191
Cuts at: 5488 826
Size: 1471
Fragments arranged by size:
1675 1471 1135 681 495 219 191 154
112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2946 3166 4237 5138 5400 820
Size: 197 926 1003 220 1071 901 262 1553
Fragments arranged by size:
1553 1071 1003 926 901 262 220 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2004 2845 2984 3111 3572 513
Size: 552 939 841 139 127 461 3074
Fragments arranged by size:
3074 939 841 552 461 139 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4160 4177 4330 4515 4960 325
Size: 1255 2580 17 153 185 445 1498
Fragments arranged by size:
2580 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4379 6091 96
Size: 274 80 33 51 1443 2402 1712 138
Fragments arranged by size:
2402 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2376 2408 6093 6125 2376
Size: 32 3685 32 2384
Fragments arranged by size:
3685 2384 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2380 1316
Size: 1064 5069
XbaI T'CTAG_A
Cuts at: 3469 3469
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI
BplI BsiWI BsmBI Bsu36I DraIII EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI