Restriction map for
pFN21AE1936
(Circular) (Six-base) MAPSORT of: pFN21AE1936.seq Check: 5008 from: 1 to: 6130
pFN21AE1936 6130 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2983 2983
Size: 6130
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3940 278
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3473 1937
Size: 1536 4594
Acc65I G'GTAC_C
Cuts at: 2179 3451 2179
Size: 1272 4858
AclI AA'CG_TT
Cuts at: 1563 4257 4630 1563
Size: 2694 373 3063
Fragments arranged by size:
3063 2694 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 3380 3389 4193 5577 5845 1632
Size: 1049 699 9 804 1384 268 1917
Fragments arranged by size:
1917 1384 1049 804 699 268 9
AfeI AGC'GCT
Cuts at: 2222 2222
Size: 6130
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5933
AflIII A'CryG_T
Cuts at: 1203 2572 5029 6006 1203
Size: 1369 2457 977 1327
Fragments arranged by size:
2457 1369 1327 977
AgeI A'CCGG_T
Cuts at: 4961 4961
Size: 6130
AhdI GACnn_n'nnGTC
Cuts at: 2368 4859 2368
Size: 2491 3639
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6130
AlwNI CAG_nnn'CTG
Cuts at: 3873 5445 3873
Size: 1572 4558
ApaLI G'TGCA_C
Cuts at: 4187 5343 4187
Size: 1156 4974
ApoI r'AATT_y
Cuts at: 1493 3439 3665 3817 5926 1493
Size: 1946 226 152 2109 1697
Fragments arranged by size:
2109 1946 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4684 160
Size: 872 3652 1606
Fragments arranged by size:
3652 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
AvaI C'yCGr_G
Cuts at: 1870 1943 2171 3455 1870
Size: 73 228 1284 4545
Fragments arranged by size:
4545 1284 228 73
AvrII C'CTAG_G
Cuts at: 2851 2851
Size: 6130
BamHI G'GATC_C
Cuts at: 3460 3827 3460
Size: 367 5763
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2179 3451 4906 618
Size: 325 217 627 392 1272 1455 1842
Fragments arranged by size:
1842 1455 1272 627 392 325 217
BanII G_rGCy'C
Cuts at: 1391 2704 3414 3449 1391
Size: 1313 710 35 4072
Fragments arranged by size:
4072 1313 710 35
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2053 928
Size: 971 154 5005
Fragments arranged by size:
5005 971 154
BbvCI CC'TCA_GC
Cuts at: 2464 2464
Size: 6130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4321 4355 1903
Size: 34 2384 34 3678
Fragments arranged by size:
3678 2384 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4022 5232 4022
Size: 1210 4920
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6130
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4741 136
Size: 107 122 71 631 605 3069 1525
Fragments arranged by size:
3069 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2269 6125 2269
Size: 3856 2274
BlpI GC'TnA_GC
Cuts at: 3547 3547
Size: 6130
Bme1580I G_kGCm'C
Cuts at: 1790 2138 4191 5347 1790
Size: 348 2053 1156 2573
Fragments arranged by size:
2573 2053 1156 348
BmgBI CAC'GTC
Cuts at: 1318 2350 1318
Size: 1032 5098
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2896 3861 4819
Size: 416 53 83 399 24 1479 965 958
Cuts at: 4819 442
Size: 1753
Fragments arranged by size:
1753 1479 965 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2713 4790 1239
Size: 1474 2077 2579
Fragments arranged by size:
2579 2077 1474
Bpu10I CC'TnA_GC
Cuts at: 2464 2464
Size: 6130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2378 3302 3398 4255 5120 5418 2378
Size: 924 96 857 865 298 3090
Fragments arranged by size:
3090 924 865 857 298 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4793 882
Size: 617 3294 2219
Fragments arranged by size:
3294 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3921 493
Size: 711 915 1802 2702
Fragments arranged by size:
2702 1802 915 711
BsaBI GATnn'nnATC
Cuts at: 2268 6124 2268
Size: 3856 2274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 4319 4954 275
Size: 53 83 186 936 2404 382 635 1451
Fragments arranged by size:
2404 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2649 4563 4961 5235 5382 5793 2649
Size: 1914 398 274 147 411 2986
Fragments arranged by size:
2986 1914 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 1178
Size: 30 165 30 777 30 5098
Fragments arranged by size:
5098 777 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6130
BseYI C'CCAG_C
Cuts at: 3397 5333 5829 3397
Size: 1936 496 3698
Fragments arranged by size:
3698 1936 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5557
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3580 4341 5369 1540
Size: 275 186 1579 761 1028 2301
Fragments arranged by size:
2301 1579 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3414 4191 4276 5347 3414
Size: 777 85 1071 4197
Fragments arranged by size:
4197 1071 777 85
BsmI GAATG_Cn'
Cuts at: 3641 3734 3641
Size: 93 6037
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2704 3414 3449 4191 4276 5347
Size: 399 348 566 710 35 742 85 1071
Cuts at: 5347 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 710 566 399 348 85
35
BspEI T'CCGG_A
Cuts at: 2649 5793 2649
Size: 3144 2986
BspHI T'CATG_A
Cuts at: 3202 4019 5749 3202
Size: 817 1730 3583
Fragments arranged by size:
3583 1730 817
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 2983 3485 844
Size: 1575 564 502 3489
Fragments arranged by size:
3489 1575 564 502
BsrBI CCG'CTC
Cuts at: 1394 1823 4017 1394
Size: 429 2194 3507
Fragments arranged by size:
3507 2194 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4625 4799 65
Size: 1183 3377 174 1396
Fragments arranged by size:
3377 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2105 4774 4961 1700
Size: 110 295 2669 187 2869
Fragments arranged by size:
2869 2669 295 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6130
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6130
BssSI C'ACGA_G
Cuts at: 4190 5202 4190
Size: 1012 5118
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2102 794
Size: 1308 4822
BstBI TT'CG_AA
Cuts at: 4934 4934
Size: 6130
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6130
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3302 2011
Size: 1291 4839
BstYI r'GATC_y
Cuts at: 1972 2269 2340 3460 3827 4212 4229 5681 5767
Size: 297 71 1120 367 385 17 1452 86
Cuts at: 5767 5779 6125 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1120 385 367 346 297 86
71 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3068 513
Size: 552 939 1064 3575
Fragments arranged by size:
3575 1064 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5391
Fragments arranged by size:
5391 683 56
BtsI GCAGTG_nn'
Cuts at: 2475 2727 3646 4440 4460 2475
Size: 252 919 794 20 4145
Fragments arranged by size:
4145 919 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6095
DraI TTT'AAA
Cuts at: 3434 3434
Size: 6130
DraIII CAC_nnn'GTG
Cuts at: 3074 3074
Size: 6130
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3133 5137 809
Size: 507 1817 2004 1802
Fragments arranged by size:
2004 1817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095
Size: 54 1425 50 275 18 1747 889 1629
Cuts at: 6095 8
Size: 43
Fragments arranged by size:
1747 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3577 1537
Size: 275 1765 4090
Fragments arranged by size:
4090 1765 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2539 2692 3334 4060 1353
Size: 1186 153 642 726 3423
Fragments arranged by size:
3423 1186 726 642 153
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3041 4717 5091 5237 387
Size: 1064 138 1452 1676 374 146 1280
Fragments arranged by size:
1676 1452 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3412 3412
Size: 6130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2681 2713 3380 3389 4193 4790 5577
Size: 393 1049 32 667 9 804 597 787
Cuts at: 5577 5845 1239
Size: 268 1524
Fragments arranged by size:
1524 1049 804 787 667 597 393 268
32 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2765 2765
Size: 6130
EcoO109I rG'GnC_Cy
Cuts at: 3349 3349
Size: 6130
EcoRI G'AATT_C
Cuts at: 3439 3817 3439
Size: 378 5752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2576 2576
Size: 6130
FspI TGC'GCA
Cuts at: 4636 4636
Size: 6130
HaeII r_GCGC'y
Cuts at: 1678 2224 5277 1678
Size: 546 3053 2531
Fragments arranged by size:
3053 2531 546
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2370 2386 2402 4771
Size: 32 135 32 782 16 16 16 2369
Cuts at: 4771 4803 4845 4877 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2369 782 135 42 32 32 32
32 16 16 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3474 3720 669
Size: 988 281 1536 246 3079
Fragments arranged by size:
3079 1536 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2558 748
Size: 1810 4320
HpaI GTT'AAC
Cuts at: 3720 3720
Size: 6130
KpnI G_GTAC'C
Cuts at: 2183 3455 2183
Size: 1272 4858
MfeI C'AATT_G
Cuts at: 3267 3729 3267
Size: 462 5668
MluI A'CGCG_T
Cuts at: 6006 6006
Size: 6130
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2374 5244 5428 1305
Size: 1069 2870 184 2007
Fragments arranged by size:
2870 2007 1069 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4308
Fragments arranged by size:
4308 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2513 3300 4088 4447 4606 518
Size: 648 843 504 787 788 359 159 2042
Fragments arranged by size:
2042 843 788 787 648 504 359 159
MspA1I CmG'CkG
Cuts at: 1606 3546 4223 5371 5616 1606
Size: 1940 677 1148 245 2120
Fragments arranged by size:
2120 1940 1148 677 245
NaeI GCC'GGC
Cuts at: 2107 2107
Size: 6130
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4639
Fragments arranged by size:
4639 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6130
NgoMIV G'CCGG_C
Cuts at: 2105 2105
Size: 6130
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6130
NotI GC'GGCC_GC
Cuts at: 3577 3577
Size: 6130
NspI r_CATG'y
Cuts at: 1132 1229 2576 3488 5033 1132
Size: 97 1347 912 1545 2229
Fragments arranged by size:
2229 1545 1347 912 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6130
PciI A'CATG_T
Cuts at: 2572 5029 2572
Size: 2457 3673
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6130
PfoI T'CCnGG_A
Cuts at: 2244 3087 2244
Size: 843 5287
PmeI GTTT'AAAC
Cuts at: 3434 3434
Size: 6130
PmlI CAC'GTG
Cuts at: 2119 2119
Size: 6130
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4199 4231 5712 5744 4199
Size: 32 1481 32 4585
Fragments arranged by size:
4585 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6130
PsiI TTA'TAA
Cuts at: 3700 3700
Size: 6130
PstI C_TGCA'G
Cuts at: 830 2340 2430 2680 3482 830
Size: 1510 90 250 802 3478
Fragments arranged by size:
3478 1510 802 250 90
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6130
SacI G_AGCT'C
Cuts at: 3414 3414
Size: 6130
SalI G'TCGA_C
Cuts at: 1936 3472 1936
Size: 1536 4594
SapI GCTCTTCn'nnn_
Cuts at: 2539 2692 2539
Size: 153 5977
SbfI CC_TGCA'GG
Cuts at: 2430 3482 2430
Size: 1052 5078
ScaI AGT'ACT
Cuts at: 2864 4378 6090 2864
Size: 1514 1712 2904
Fragments arranged by size:
2904 1712 1514
SfcI C'TryA_G
Cuts at: 826 1045 2336 2426 2676 3478 4613 5294 5485
Size: 219 1291 90 250 802 1135 681 191
Cuts at: 5485 826
Size: 1471
Fragments arranged by size:
1471 1291 1135 802 681 250 219 191
90
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6130
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6130
SmaI CCC'GGG
Cuts at: 1872 2173 3457 1872
Size: 301 1284 4545
Fragments arranged by size:
4545 1284 301
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2357 3317 3413 4234 5135 5397
Size: 197 926 414 960 96 821 901 262
Cuts at: 5397 820
Size: 1553
Fragments arranged by size:
1553 960 926 901 821 414 262 197
96
SnaBI TAC'GTA
Cuts at: 493 3921 493
Size: 3428 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6130
SphI G_CATG'C
Cuts at: 1132 3488 1132
Size: 2356 3774
SspI AAT'ATT
Cuts at: 5 52 4054 5
Size: 47 4002 2081
Fragments arranged by size:
4002 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6130
StyI C'CwwG_G
Cuts at: 513 1065 2004 2184 2365 2851 3391 3569 513
Size: 552 939 180 181 486 540 178 3074
Fragments arranged by size:
3074 939 552 540 486 181 180 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3170 4157 4174 4327 4512 4957 325
Size: 1255 1590 987 17 153 185 445 1498
Fragments arranged by size:
1590 1498 1255 987 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2862 4376 6088
Size: 274 80 33 51 1443 885 1514 1712
Cuts at: 6088 96
Size: 138
Fragments arranged by size:
1712 1514 1443 885 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 2731 2763 6090 6122 2180
Size: 32 519 32 3327 32 2188
Fragments arranged by size:
3327 2188 519 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3003 1316
Size: 1687 4443
XbaI T'CTAG_A
Cuts at: 3466 3466
Size: 6130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3164 3164
Size: 6130
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6130
XmaI C'CCGG_G
Cuts at: 1870 2171 3455 1870
Size: 301 1284 4545
Fragments arranged by size:
4545 1284 301
XmnI GAAnn'nnTTC
Cuts at: 2559 4259 2559
Size: 1700 4430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3938 276
Size: 53 83 186 936 2404 2468
Fragments arranged by size:
2468 2404 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PstI PvuI SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI
NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI