Restriction map for
pFN21AE1933
(Circular) (Six-base) MAPSORT of: pFN21AE1933.seq Check: 1539 from: 1 to: 6115
pFN21AE1933 6115 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3105 3105
Size: 6115
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2475 3458 1937
Size: 538 983 4594
Fragments arranged by size:
4594 983 538
Acc65I G'GTAC_C
Cuts at: 2060 3436 2060
Size: 1376 4739
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 2649 3252 3330 4178 5562 5830 1632
Size: 525 492 603 78 848 1384 268 1917
Fragments arranged by size:
1917 1384 848 603 525 492 268 78
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 2862 5014 5991 1203
Size: 1659 2152 977 1327
Fragments arranged by size:
2152 1659 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 2121 4844 2121
Size: 2723 3392
AleI CACnn'nnGTG
Cuts at: 1166 2754 1166
Size: 1588 4527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6115
AlwNI CAG_nnn'CTG
Cuts at: 2143 2513 2791 2933 3858 5430 2143
Size: 370 278 142 925 1572 2828
Fragments arranged by size:
2828 1572 925 370 278 142
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2491 2847 3440 1870
Size: 73 548 356 593 4545
Fragments arranged by size:
4545 593 548 356 73
BamHI G'GATC_C
Cuts at: 3445 3812 3445
Size: 367 5748
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2030 2060 2669 2838 3436
Size: 325 217 627 243 30 609 169 598
Cuts at: 3436 4891 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 609 598 325 243 217
169 30
BanII G_rGCy'C
Cuts at: 1391 2023 2874 3434 1391
Size: 632 851 560 4072
Fragments arranged by size:
4072 851 632 560
BbeI G_GCGC'C
Cuts at: 2842 2842
Size: 6115
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5144
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4306 4340 1903
Size: 34 2369 34 3678
Fragments arranged by size:
3678 2369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2687 4726 136
Size: 107 122 71 631 605 1015 2039 1525
Fragments arranged by size:
2039 1525 1015 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 3003 3532 3003
Size: 529 5586
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2683 4176 5332 1790
Size: 245 648 1493 1156 2573
Fragments arranged by size:
2573 1493 1156 648 245
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2024 3846 4804
Size: 416 53 83 399 24 607 1822 958
Cuts at: 4804 442
Size: 1753
Fragments arranged by size:
1822 1753 958 607 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2430 2584 2430
Size: 154 5961
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 2449 2659 2705 2712 2960 3062 3197
Size: 1005 205 210 46 7 248 102 135
Cuts at: 3197 3359 4775 1239
Size: 162 1416 2579
Fragments arranged by size:
2579 1416 1005 248 210 205 162 135
102 46 7
Bpu10I CC'TnA_GC
Cuts at: 3140 3140
Size: 6115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4240 5105 5403 4240
Size: 865 298 4952
Fragments arranged by size:
4952 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3136 4778 882
Size: 617 1637 1642 2219
Fragments arranged by size:
2219 1642 1637 617
BsaAI yAC'GTr
Cuts at: 493 1204 2191 3906 493
Size: 711 987 1715 2702
Fragments arranged by size:
2702 1715 987 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2185 2839 3922 4304
Size: 53 83 186 936 652 654 1083 382
Cuts at: 4304 4939 275
Size: 635 1451
Fragments arranged by size:
1451 1083 936 654 652 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4548 4946 5220 5367 5778 4548
Size: 398 274 147 411 4885
Fragments arranged by size:
4885 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2461 2479 2894 1186
Size: 1159 116 18 415 4407
Fragments arranged by size:
4407 1159 415 116 18
BseYI C'CCAG_C
Cuts at: 2044 2056 2118 2243 5318 5814 2044
Size: 12 62 125 3075 496 2345
Fragments arranged by size:
3075 2345 496 125 62 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2732 1163
Size: 573 996 4546
Fragments arranged by size:
4546 996 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3565 4326 5354 1540
Size: 275 186 1564 761 1028 2301
Fragments arranged by size:
2301 1564 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2874 4176 4261 5332 2874
Size: 1302 85 1071 3657
Fragments arranged by size:
3657 1302 1071 85
BsmI GAATG_Cn'
Cuts at: 3626 3719 3626
Size: 93 6022
BsmBI CGTCTCn'nnnn_
Cuts at: 3405 3405
Size: 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2035 2683 2874 3434 4176 4261
Size: 399 233 12 648 191 560 742 85
Cuts at: 4261 5332 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 648 560 399 233 191
85 12
BspEI T'CCGG_A
Cuts at: 5778 5778
Size: 6115
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3105 3470 844
Size: 2261 365 3489
Fragments arranged by size:
3489 2261 365
BsrBI CCG'CTC
Cuts at: 1394 1823 2563 4002 1394
Size: 429 740 1439 3507
Fragments arranged by size:
3507 1439 740 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3069 4610 4784 65
Size: 1183 1821 1541 174 1396
Fragments arranged by size:
1821 1541 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2069 2820 4759 4946 1700
Size: 110 259 751 1939 187 2869
Fragments arranged by size:
2869 1939 751 259 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 4175 5187 4175
Size: 1012 5103
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2933 3120 794
Size: 2139 187 3789
Fragments arranged by size:
3789 2139 187
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstXI CCAn_nnnn'nTGG
Cuts at: 2126 2155 2308 2683 2126
Size: 29 153 375 5558
Fragments arranged by size:
5558 375 153 29
BstYI r'GATC_y
Cuts at: 1972 2176 3445 3812 4197 4214 5666 5752 5764
Size: 204 1269 367 385 17 1452 86 12
Cuts at: 5764 6110 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1269 385 367 346 204 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2701 2701
Size: 6115
BtgI C'CryG_G
Cuts at: 513 1065 2252 3093 513
Size: 552 1187 841 3535
Fragments arranged by size:
3535 1187 841 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2119 487
Size: 683 56 893 4483
Fragments arranged by size:
4483 893 683 56
BtsI GCAGTG_nn'
Cuts at: 3631 4425 4445 3631
Size: 794 20 5301
Fragments arranged by size:
5301 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2128 3562 4451
Size: 54 1425 50 275 18 298 1434 889
Cuts at: 4451 6080 8
Size: 1629 43
Fragments arranged by size:
1629 1434 1425 889 298 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3562 1537
Size: 275 1750 4090
Fragments arranged by size:
4090 1750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2391 2649 4045 1353
Size: 1038 258 1396 3423
Fragments arranged by size:
3423 1396 1038 258
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4702 5076 5222 387
Size: 1064 138 3113 374 146 1280
Fragments arranged by size:
3113 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2872 2872
Size: 6115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2157 2244 2449 2649 2659 2705 2712
Size: 393 525 87 205 200 10 46 7
Cuts at: 2712 2960 3062 3197 3252 3330 3359 4178 4775
Size: 248 102 135 55 78 29 819 597
Cuts at: 4775 5562 5830 1239
Size: 787 268 1524
Fragments arranged by size:
1524 819 787 597 525 393 268 248
205 200 135 102 87 78 55 46
29 10 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2732 2732
Size: 6115
EcoO109I rG'GnC_Cy
Cuts at: 3382 3382
Size: 6115
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 2315 2842 5262 1678
Size: 637 527 2420 2531
Fragments arranged by size:
2531 2420 637 527
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2161 2193 2882
Size: 32 135 32 535 32 22 32 689
Cuts at: 2882 2914 4756 4788 4830 4862 1373
Size: 32 1842 32 42 32 2626
Fragments arranged by size:
2626 1842 689 535 135 42 32 32
32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6115
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KasI G'GCGC_C
Cuts at: 2838 2838
Size: 6115
KpnI G_GTAC'C
Cuts at: 2064 3440 2064
Size: 1376 4739
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5229 5413 1305
Size: 3924 184 2007
Fragments arranged by size:
3924 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4293
Fragments arranged by size:
4293 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2754 4073 4432 4591 518
Size: 648 1588 1319 359 159 2042
Fragments arranged by size:
2042 1588 1319 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2788 2998 3531 4208 5356 5601 1606
Size: 1182 210 533 677 1148 245 2120
Fragments arranged by size:
2120 1182 1148 677 533 245 210
NaeI GCC'GGC
Cuts at: 2071 2822 2071
Size: 751 5364
NarI GG'CG_CC
Cuts at: 2839 2839
Size: 6115
NcoI C'CATG_G
Cuts at: 513 1065 3093 513
Size: 552 2028 3535
Fragments arranged by size:
3535 2028 552
NdeI CA'TA_TG
Cuts at: 387 2902 387
Size: 2515 3600
NgoMIV G'CCGG_C
Cuts at: 2069 2820 2069
Size: 751 5364
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 2866 3059 3473 5018 1132
Size: 97 1637 193 414 1545 2229
Fragments arranged by size:
2229 1637 1545 414 193 97
PasI CC'CwG_GG
Cuts at: 1348 2023 1348
Size: 675 5440
PciI A'CATG_T
Cuts at: 2862 5014 2862
Size: 2152 3963
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2410 1108
Size: 1302 4813
PfoI T'CCnGG_A
Cuts at: 2179 2439 2179
Size: 260 5855
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PmlI CAC'GTG
Cuts at: 2191 2191
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3382 3382
Size: 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2733 3467 830
Size: 1903 734 3478
Fragments arranged by size:
3478 1903 734
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2788 2998 2788
Size: 210 5905
SacI G_AGCT'C
Cuts at: 2874 2874
Size: 6115
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SbfI CC_TGCA'GG
Cuts at: 3467 3467
Size: 6115
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2729 2854 3165 3239 3286 3463 4598
Size: 219 1684 125 311 74 47 177 1135
Cuts at: 4598 5279 5470 826
Size: 681 191 1471
Fragments arranged by size:
1684 1471 1135 681 311 219 191 177
125 74 47
SfoI GGC'GCC
Cuts at: 2840 2840
Size: 6115
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 3442 1872
Size: 1570 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4219 5120 5382 820
Size: 197 926 2276 901 262 1553
Fragments arranged by size:
2276 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 3473 1132
Size: 2341 3774
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 2074 3093 3554 513
Size: 552 1009 1019 461 3074
Fragments arranged by size:
3074 1019 1009 552 461
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2015 4142 4159 4312 4497 4942 325
Size: 1255 435 2127 17 153 185 445 1498
Fragments arranged by size:
2127 1498 1255 445 435 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2175 2207 6075 6107 2175
Size: 32 3868 32 2183
Fragments arranged by size:
3868 2183 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 2009 3451 2009
Size: 1442 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 3440 1870
Size: 1570 4545
XmnI GAAnn'nnTTC
Cuts at: 2635 4244 2635
Size: 1609 4506
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SrfI SwaI