Restriction map for
pFN21AE1932
(Circular) (Six-base) MAPSORT of: pFN21AE1932.seq Check: 7858 from: 1 to: 6115
pFN21AE1932 6115 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3458 1937
Size: 1521 4594
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2948 4178 5562 5830 1632
Size: 1316 1230 1384 268 1917
Fragments arranged by size:
1917 1384 1316 1230 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 4399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2055 1184
Size: 871 5244
AlwNI CAG_nnn'CTG
Cuts at: 2785 3858 5430 2785
Size: 1073 1572 3470
Fragments arranged by size:
3470 1572 1073
ApaI G_GGCC'C
Cuts at: 2325 2325
Size: 6115
ApaLI G'TGCA_C
Cuts at: 4172 5328 4172
Size: 1156 4959
ApoI r'AATT_y
Cuts at: 1493 2120 2447 2606 3424 3650 3802 5911 1493
Size: 627 327 159 818 226 152 2109 1697
Fragments arranged by size:
2109 1697 818 627 327 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 3440 1870
Size: 73 1497 4545
Fragments arranged by size:
4545 1497 73
BamHI G'GATC_C
Cuts at: 3445 3812 3445
Size: 367 5748
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3436 4891 618
Size: 325 217 627 1649 1455 1842
Fragments arranged by size:
1842 1649 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2053 2325 2506 3434 1391
Size: 662 272 181 928 4072
Fragments arranged by size:
4072 928 662 272 181
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3292 928
Size: 971 1393 3751
Fragments arranged by size:
3751 1393 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2255 2289 4306 4340 1903
Size: 34 318 34 2017 34 3678
Fragments arranged by size:
3678 2017 318 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2320 4726 136
Size: 107 122 71 631 605 648 2406 1525
Fragments arranged by size:
2406 1525 648 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2895 6110 2895
Size: 3215 2900
BlpI GC'TnA_GC
Cuts at: 3532 3532
Size: 6115
Bme1580I G_kGCm'C
Cuts at: 1790 2325 4176 5332 1790
Size: 535 1851 1156 2573
Fragments arranged by size:
2573 1851 1156 535
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3846 4804 442
Size: 416 53 83 399 24 2429 958 1753
Fragments arranged by size:
2429 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2067 2276 4775 1239
Size: 828 209 2499 2579
Fragments arranged by size:
2579 2499 828 209
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 4240 5105 5403 2153
Size: 2087 865 298 2865
Fragments arranged by size:
2865 2087 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2787 2935 4778 882
Size: 617 1288 148 1843 2219
Fragments arranged by size:
2219 1843 1288 617 148
BsaAI yAC'GTr
Cuts at: 493 1204 2771 3906 493
Size: 711 1567 1135 2702
Fragments arranged by size:
2702 1567 1135 711
BsaBI GATnn'nnATC
Cuts at: 2295 6109 2295
Size: 3814 2301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3922 4304 4939 275
Size: 53 83 186 936 2389 382 635 1451
Fragments arranged by size:
2389 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2406 4548 4946 5220 5367 5778 2406
Size: 2142 398 274 147 411 2743
Fragments arranged by size:
2743 2142 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6115
BseYI C'CCAG_C
Cuts at: 5318 5814 5318
Size: 496 5619
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5542
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3565 4326 5354 1540
Size: 275 186 1564 761 1028 2301
Fragments arranged by size:
2301 1564 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2053 2506 4176 4261 5332 2053
Size: 453 1670 85 1071 2836
Fragments arranged by size:
2836 1670 1071 453 85
BsmI GAATG_Cn'
Cuts at: 2775 3626 3719 2775
Size: 851 93 5171
Fragments arranged by size:
5171 851 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 2325 2506 3434 4176 4261 5332
Size: 399 263 272 181 928 742 85 1071
Cuts at: 5332 1391
Size: 2174
Fragments arranged by size:
2174 1071 928 742 399 272 263 181
85
BspEI T'CCGG_A
Cuts at: 2406 5778 2406
Size: 3372 2743
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3470 844
Size: 2626 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4002 1394
Size: 429 2179 3507
Fragments arranged by size:
3507 2179 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4610 4784 65
Size: 1183 3362 174 1396
Fragments arranged by size:
3362 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4759 4946 1700
Size: 110 2949 187 2869
Fragments arranged by size:
2949 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 2657 4175 5187 2657
Size: 1518 1012 3585
Fragments arranged by size:
3585 1518 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6115
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6115
BstXI CCAn_nnnn'nTGG
Cuts at: 2558 2558
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2567 2895 3338 3445 3812 4197 4214 5666
Size: 595 328 443 107 367 385 17 1452
Cuts at: 5666 5752 5764 6110 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 595 443 385 367 346 328
107 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2674 2674
Size: 6115
BtgI C'CryG_G
Cuts at: 513 1065 2497 2883 513
Size: 552 1432 386 3745
Fragments arranged by size:
3745 1432 552 386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 2037 2646 3631 4425 4445 2037
Size: 609 985 794 20 3707
Fragments arranged by size:
3707 985 794 609 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3046 3419 3046
Size: 373 5742
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2665 2683 2978
Size: 54 1425 50 275 18 835 18 295
Cuts at: 2978 3562 4451 6080 8
Size: 584 889 1629 43
Fragments arranged by size:
1629 1425 889 835 584 295 275 54
50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3562 1537
Size: 275 1750 4090
Fragments arranged by size:
4090 1750 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2058 4045 1353
Size: 705 1987 3423
Fragments arranged by size:
3423 1987 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4702 5076 5222 387
Size: 1064 138 3113 374 146 1280
Fragments arranged by size:
3113 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2051 2504 2051
Size: 453 5662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2067 2276 2948 4178 4775 5562 5830
Size: 393 435 209 672 1230 597 787 268
Cuts at: 5830 1239
Size: 1524
Fragments arranged by size:
1524 1230 787 672 597 435 393 268
209
EcoO109I rG'GnC_Cy
Cuts at: 2321 3177 2321
Size: 856 5259
EcoRI G'AATT_C
Cuts at: 2606 3424 3802 2606
Size: 818 378 4919
Fragments arranged by size:
4919 818 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3056 3056
Size: 6115
FspI TGC'GCA
Cuts at: 4621 4621
Size: 6115
HaeII r_GCGC'y
Cuts at: 1678 5262 1678
Size: 3584 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 4756 4788 4830
Size: 32 135 32 696 32 2456 32 42
Cuts at: 4830 4862 1373
Size: 32 2626
Fragments arranged by size:
2626 2456 696 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2395 2734 3459 3705 669
Size: 988 281 457 339 725 246 3079
Fragments arranged by size:
3079 988 725 457 339 281 246
HindIII A'AGCT_T
Cuts at: 748 2412 748
Size: 1664 4451
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3394 5229 5413 1305
Size: 2089 1835 184 2007
Fragments arranged by size:
2089 2007 1835 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2667 2685 2980 10
Size: 54 1425 343 835 18 295 3145
Fragments arranged by size:
3145 1425 835 343 295 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2275 2468 2483 2595 2691 2882 4073
Size: 648 1109 193 15 112 96 191 1191
Cuts at: 4073 4432 4591 518
Size: 359 159 2042
Fragments arranged by size:
2042 1191 1109 648 359 193 191 159
112 96 15
MspA1I CmG'CkG
Cuts at: 1606 3152 3531 4208 5356 5601 1606
Size: 1546 379 677 1148 245 2120
Fragments arranged by size:
2120 1546 1148 677 379 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5563
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6115
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 3473 5018 1132
Size: 97 2244 1545 2229
Fragments arranged by size:
2244 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6115
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6115
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2829 2861 4184 4216 5697 5729 2829
Size: 32 1323 32 1481 32 3215
Fragments arranged by size:
3215 1481 1323 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3177 3177
Size: 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspOMI G'GGCC_C
Cuts at: 2321 2321
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2034 3467 830
Size: 1204 1433 3478
Fragments arranged by size:
3478 1433 1204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 3152 3152
Size: 6115
SacI G_AGCT'C
Cuts at: 2053 2506 2053
Size: 453 5662
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SapI GCTCTTCn'nnn_
Cuts at: 2058 2058
Size: 6115
SbfI CC_TGCA'GG
Cuts at: 2034 3467 2034
Size: 1433 4682
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2030 2350 3463 4598 5279 5470 826
Size: 219 985 320 1113 1135 681 191 1471
Fragments arranged by size:
1471 1135 1113 985 681 320 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6115
SmaI CCC'GGG
Cuts at: 1872 3442 1872
Size: 1570 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2132 4219 5120 5382 820
Size: 197 926 189 2087 901 262 1553
Fragments arranged by size:
2087 1553 926 901 262 197 189
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 3473 1132
Size: 2341 3774
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6115
StyI C'CwwG_G
Cuts at: 513 1065 3271 3334 3554 513
Size: 552 2206 63 220 3074
Fragments arranged by size:
3074 2206 552 220 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4142 4159 4312 4497 4942 325
Size: 1255 2562 17 153 185 445 1498
Fragments arranged by size:
2562 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6075 6107 6075
Size: 32 6083
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2525 2525
Size: 6115
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 3440 1870
Size: 1570 4545
XmnI GAAnn'nnTTC
Cuts at: 2414 4244 2414
Size: 1830 4285
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI