Restriction map for
pFN21AE1925
(Circular) (Six-base) MAPSORT of: pFN21AE1925.seq Check: 3165 from: 1 to: 6091
pFN21AE1925 6091 bp
With 182 enzymes: SgfI *
March 28, 2012 11:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 2356
Size: 6091
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2691 3434 1937
Size: 754 743 4594
Fragments arranged by size:
4594 754 743
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2507 3187 3367 4154 5538 5806 1632
Size: 875 680 180 787 1384 268 1917
Fragments arranged by size:
1917 1384 875 787 680 268 180
AfeI AGC'GCT
Cuts at: 3140 3140
Size: 6091
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 2770 4820 2770
Size: 2050 4041
AleI CACnn'nnGTG
Cuts at: 1166 2201 2519 3149 1166
Size: 1035 318 630 4108
Fragments arranged by size:
4108 1035 630 318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2448 1184
Size: 1264 4827
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaI G_GGCC'C
Cuts at: 2057 2762 3027 2057
Size: 705 265 5121
Fragments arranged by size:
5121 705 265
ApaLI G'TGCA_C
Cuts at: 4148 5304 4148
Size: 1156 4935
ApoI r'AATT_y
Cuts at: 1493 3400 3626 3778 5887 1493
Size: 1907 226 152 2109 1697
Fragments arranged by size:
2109 1907 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 3019 3416 1870
Size: 73 164 912 397 4545
Fragments arranged by size:
4545 912 397 164 73
BamHI G'GATC_C
Cuts at: 3421 3788 3421
Size: 367 5724
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2591 2892 3084 3244 3412
Size: 325 217 627 804 301 192 160 168
Cuts at: 3412 4867 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 804 627 325 301 217 192
168 160
BanII G_rGCy'C
Cuts at: 1391 2057 2343 2762 3027 3410 1391
Size: 666 286 419 265 383 4072
Fragments arranged by size:
4072 666 419 383 286 265
BbeI G_GCGC'C
Cuts at: 2595 2896 3088 2595
Size: 301 192 5598
Fragments arranged by size:
5598 301 192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2185 3313 928
Size: 971 286 1128 3706
Fragments arranged by size:
3706 1128 971 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3983 5193 3983
Size: 1210 4881
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6091
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2111 3219 4702
Size: 107 122 71 631 605 439 1108 1483
Cuts at: 4702 136
Size: 1525
Fragments arranged by size:
1525 1483 1108 631 605 439 122 107
71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 2735 3508 2735
Size: 773 5318
Bme1580I G_kGCm'C
Cuts at: 1790 2057 2762 2829 3001 3027 3249 4152 5308
Size: 267 705 67 172 26 222 903 1156
Cuts at: 5308 1790
Size: 2573
Fragments arranged by size:
2573 1156 903 705 267 222 172 67
26
BmgBI CAC'GTC
Cuts at: 1318 2851 1318
Size: 1533 4558
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3822 4780 442
Size: 416 53 83 399 24 2405 958 1753
Fragments arranged by size:
2405 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2995 4751 1239
Size: 1756 1756 2579
Fragments arranged by size:
2579 1756 1756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4216 5081 5379 4216
Size: 865 298 4928
Fragments arranged by size:
4928 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2572 2618 4754 882
Size: 617 1073 46 2136 2219
Fragments arranged by size:
2219 2136 1073 617 46
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2592 2893 3085
Size: 53 83 186 936 476 583 301 192
Cuts at: 3085 3898 4280 4915 275
Size: 813 382 635 1451
Fragments arranged by size:
1451 936 813 635 583 476 382 301
192 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5866
Fragments arranged by size:
5866 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3385 1186
Size: 2199 3892
BseYI C'CCAG_C
Cuts at: 2794 5294 5790 2794
Size: 2500 496 3095
Fragments arranged by size:
3095 2500 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5518
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2292 2421 2544 2880 3541 4302
Size: 275 186 291 129 123 336 661 761
Cuts at: 4302 5330 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 661 336 291 275 186
129 123
BsiHKAI G_wGCw'C
Cuts at: 2343 3183 4152 4237 5308 2343
Size: 840 969 85 1071 3126
Fragments arranged by size:
3126 1071 969 840 85
BsmI GAATG_Cn'
Cuts at: 3602 3695 3602
Size: 93 5998
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2343 2762 2829 3001 3027 3183
Size: 399 267 286 419 67 172 26 156
Cuts at: 3183 3249 3410 4152 4237 5308 1391
Size: 66 161 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 419 399 286 267 172
161 156 85 67 66 26
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 3980 5710 3980
Size: 1730 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 3379 3446 844
Size: 1512 1023 67 3489
Fragments arranged by size:
3489 1512 1023 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2067 2930 3256 3978 1394
Size: 429 244 863 326 722 3507
Fragments arranged by size:
3507 863 722 429 326 244
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3081 4735 4922 1700
Size: 110 1271 1654 187 2869
Fragments arranged by size:
2869 1654 1271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 2251 2253 2696 2746 2832 3043 3092 3128
Size: 326 2 443 50 86 211 49 36
Cuts at: 3128 1925
Size: 4888
Fragments arranged by size:
4888 443 326 211 86 50 49 36
2
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3105 794
Size: 2311 3780
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstXI CCAn_nnnn'nTGG
Cuts at: 2443 2521 2443
Size: 78 6013
BstYI r'GATC_y
Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086
Size: 1449 367 385 17 1452 86 12 346
Cuts at: 6086 1972
Size: 1977
Fragments arranged by size:
1977 1452 1449 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2199 2754 513
Size: 552 939 168 27 555 3850
Fragments arranged by size:
3850 939 555 552 168 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 2827 3607 4401 4421 2612
Size: 215 780 794 20 4282
Fragments arranged by size:
4282 794 780 215 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 2289 2304
Size: 54 1425 50 275 18 304 155 15
Cuts at: 2304 2331 2523 2541 2712 2877 3031 3538 4427
Size: 27 192 18 171 165 154 507 889
Cuts at: 4427 6056 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 507 304 275 192 171
165 155 154 54 50 43 27 18
18 15
EagI C'GGCC_G
Cuts at: 1537 1812 2289 2541 2877 3538 1537
Size: 275 477 252 336 661 4090
Fragments arranged by size:
4090 661 477 336 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 3263 3365 4021 1353
Size: 920 990 102 656 3423
Fragments arranged by size:
3423 990 920 656 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4678 5052 5198 387
Size: 1064 138 3089 374 146 1280
Fragments arranged by size:
3089 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2341 2341
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2507 2995 3187 3367 4154 4751 5538
Size: 393 875 488 192 180 787 597 787
Cuts at: 5538 5806 1239
Size: 268 1524
Fragments arranged by size:
1524 875 787 787 597 488 393 268
192 180
EcoO109I rG'GnC_Cy
Cuts at: 2054 2759 3015 3023 2054
Size: 705 256 8 5122
Fragments arranged by size:
5122 705 256 8
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 6091
FspI TGC'GCA
Cuts at: 2723 4597 2723
Size: 1874 4217
HaeII r_GCGC'y
Cuts at: 1678 2021 2033 2249 2393 2595 2896 3088 3142
Size: 343 12 216 144 202 301 192 54
Cuts at: 3142 5238 1678
Size: 2096 2531
Fragments arranged by size:
2531 2096 343 301 216 202 192 144
54 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 2756 2788 4732
Size: 32 135 32 838 32 314 32 1944
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1944 838 314 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3435 3681 669
Size: 988 281 1497 246 3079
Fragments arranged by size:
3079 1497 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KasI G'GCGC_C
Cuts at: 2591 2892 3084 2591
Size: 301 192 5598
Fragments arranged by size:
5598 301 192
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3388 5205 5389 1305
Size: 2083 1817 184 2007
Fragments arranged by size:
2083 2007 1817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 2333 2714 10
Size: 54 1425 343 474 27 381 3387
Fragments arranged by size:
3387 1425 474 381 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2300 2430 2519 3149 3170 4049
Size: 648 1035 99 130 89 630 21 879
Cuts at: 4049 4408 4567 518
Size: 359 159 2042
Fragments arranged by size:
2042 1035 879 648 630 359 159 130
99 89 21
MspA1I CmG'CkG
Cuts at: 1606 2048 2088 2563 2876 3507 4184 5332 5577
Size: 442 40 475 313 631 677 1148 245
Cuts at: 5577 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 631 475 442 313 245
40
NaeI GCC'GGC
Cuts at: 3083 3083
Size: 6091
NarI GG'CG_CC
Cuts at: 2592 2893 3085 2592
Size: 301 192 5598
Fragments arranged by size:
5598 301 192
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4600
Fragments arranged by size:
4600 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NgoMIV G'CCGG_C
Cuts at: 3081 3081
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3449 4994 1132
Size: 97 2220 1545 2229
Fragments arranged by size:
2229 2220 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6091
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3015 3015
Size: 6091
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PspOMI G'GGCC_C
Cuts at: 2053 2758 3023 2053
Size: 705 265 5121
Fragments arranged by size:
5121 705 265
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2421 2001
Size: 420 5671
SacI G_AGCT'C
Cuts at: 2343 2343
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 3263 3263
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 2141 4339 6051 2141
Size: 2198 1712 2181
Fragments arranged by size:
2198 2181 1712
SexAI A'CCwGG_T
Cuts at: 2627 2627
Size: 6091
SfcI C'TryA_G
Cuts at: 826 1045 3208 3439 4574 5255 5446 826
Size: 219 2163 231 1135 681 191 1471
Fragments arranged by size:
2163 1471 1135 681 231 219 191
SfoI GGC'GCC
Cuts at: 2593 2894 3086 2593
Size: 301 192 5598
Fragments arranged by size:
5598 301 192
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 3081 1700
Size: 1381 4710
SmaI CCC'GGG
Cuts at: 1872 3021 3418 1872
Size: 1149 397 4545
Fragments arranged by size:
4545 1149 397
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4195 5096 5358 820
Size: 197 926 2252 901 262 1553
Fragments arranged by size:
2252 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 4015 5
Size: 47 3963 2081
Fragments arranged by size:
3963 2081 47
StuI AGG'CCT
Cuts at: 1517 3236 1517
Size: 1719 4372
StyI C'CwwG_G
Cuts at: 513 1065 2004 3530 513
Size: 552 939 1526 3074
Fragments arranged by size:
3074 1526 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4118 4135 4288 4473 4918 325
Size: 1255 2538 17 153 185 445 1498
Fragments arranged by size:
2538 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2139 2478 4337
Size: 274 80 33 51 1443 162 339 1859
Cuts at: 4337 6049 96
Size: 1712 138
Fragments arranged by size:
1859 1712 1443 339 274 162 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2341 2341
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3019 3416 1870
Size: 1149 397 4545
Fragments arranged by size:
4545 1149 397
XmnI GAAnn'nnTTC
Cuts at: 2267 3140 4220 2267
Size: 873 1080 4138
Fragments arranged by size:
4138 1080 873
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
PvuII RsrII SacII SanDI SfiI SrfI SwaI