Restriction map for
pFN21AE1922
(Circular) (Six-base) MAPSORT of: pFN21AE1922.seq Check: 6705 from: 1 to: 6007
pFN21AE1922 6007 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 2890 3328 2890
Size: 438 5569
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3191 4070 5454 5722 1632
Size: 1559 879 1384 268 1917
Fragments arranged by size:
1917 1559 1384 879 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 4906 5883 1203
Size: 3703 977 1327
Fragments arranged by size:
3703 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AlwNI CAG_nnn'CTG
Cuts at: 3088 3750 5322 3088
Size: 662 1572 3773
Fragments arranged by size:
3773 1572 662
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 2037 2592 2848 3235 3316 3542 3694 5803
Size: 544 555 256 387 81 226 152 2109
Cuts at: 5803 1493
Size: 1697
Fragments arranged by size:
2109 1697 555 544 387 256 226 152
81
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 3332 1870
Size: 73 1389 4545
Fragments arranged by size:
4545 1389 73
BamHI G'GATC_C
Cuts at: 2612 3198 3337 3704 2612
Size: 586 139 367 4915
Fragments arranged by size:
4915 586 367 139
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2890 3328 4783 618
Size: 325 217 627 579 524 438 1455 1842
Fragments arranged by size:
1842 1455 627 579 524 438 325 217
BanII G_rGCy'C
Cuts at: 1391 3148 3326 1391
Size: 1757 178 4072
Fragments arranged by size:
4072 1757 178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3182 928
Size: 971 1283 3753
Fragments arranged by size:
3753 1283 971
BbvCI CC'TCA_GC
Cuts at: 2529 2529
Size: 6007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2096 2130 2936 2970 4198 4232 1903
Size: 34 159 34 806 34 1228 34 3678
Fragments arranged by size:
3678 1228 806 159 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3899 5109 3899
Size: 1210 4797
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6007
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2270 6002 2270
Size: 3732 2275
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 4068 5224 1790
Size: 2278 1156 2573
Fragments arranged by size:
2573 2278 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2067 2496 3020
Size: 416 53 83 399 24 650 429 524
Cuts at: 3020 3738 4696 442
Size: 718 958 1753
Fragments arranged by size:
1753 958 718 650 524 429 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2289 3213 4667 1239
Size: 1050 924 1454 2579
Fragments arranged by size:
2579 1454 1050 924
Bpu10I CC'TnA_GC
Cuts at: 2529 2529
Size: 6007
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2443 4132 4997 5295 2443
Size: 1689 865 298 3155
Fragments arranged by size:
3155 1689 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2205 4670 882
Size: 617 706 2465 2219
Fragments arranged by size:
2465 2219 706 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 6001 6001
Size: 6007
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3814 4196 4831 275
Size: 53 83 186 936 2281 382 635 1451
Fragments arranged by size:
2281 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5782
Fragments arranged by size:
5782 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2049 1186
Size: 863 5144
BseYI C'CCAG_C
Cuts at: 5210 5706 5210
Size: 496 5511
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2056 4068 4153 5224 2056
Size: 2012 85 1071 2839
Fragments arranged by size:
2839 2012 1071 85
BsmI GAATG_Cn'
Cuts at: 3518 3611 3518
Size: 93 5914
BsmBI CGTCTCn'nnnn_
Cuts at: 2097 2097
Size: 6007
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2056 3148 3326 4068 4153 5224 1391
Size: 399 266 1092 178 742 85 1071 2174
Fragments arranged by size:
2174 1092 1071 742 399 266 178 85
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2878 3295 3362 844
Size: 2034 417 67 3489
Fragments arranged by size:
3489 2034 417 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2197 3894 1394
Size: 429 374 1697 3507
Fragments arranged by size:
3507 1697 429 374
BsrDI GCAATG_nn'
Cuts at: 65 1248 3265 4502 4676 65
Size: 1183 2017 1237 174 1396
Fragments arranged by size:
2017 1396 1237 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 3262 96
Size: 3166 2841
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 4067 5079 4067
Size: 1012 4995
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3088 794
Size: 2294 3713
BstBI TT'CG_AA
Cuts at: 4811 4811
Size: 6007
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstYI r'GATC_y
Cuts at: 1972 2270 2612 2806 3198 3337 3704 4089 4106
Size: 298 342 194 392 139 367 385 17
Cuts at: 4106 5558 5644 5656 6002 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 392 385 367 346 342 298
194 139 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5455
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5972
DraI TTT'AAA
Cuts at: 3311 3311
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2959 5014 809
Size: 507 1643 2055 1802
Fragments arranged by size:
2055 1802 1643 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2245 3454 4343
Size: 54 1425 50 275 18 415 1209 889
Cuts at: 4343 5972 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1209 889 415 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2182 2943 3937 1353
Size: 829 761 994 3423
Fragments arranged by size:
3423 994 829 761
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2289 3191 3213 4070 4667 5454 5722
Size: 393 657 902 22 857 597 787 268
Cuts at: 5722 1239
Size: 1524
Fragments arranged by size:
1524 902 857 787 657 597 393 268
22
EcoO109I rG'GnC_Cy
Cuts at: 3017 3058 3076 3017
Size: 41 18 5948
Fragments arranged by size:
5948 41 18
EcoRI G'AATT_C
Cuts at: 2037 3316 3694 2037
Size: 1279 378 4350
Fragments arranged by size:
4350 1279 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2466 2466
Size: 6007
FspI TGC'GCA
Cuts at: 2958 4513 2958
Size: 1555 4452
HaeII r_GCGC'y
Cuts at: 1678 5154 1678
Size: 3476 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2597 2629 4648 4680 4722
Size: 32 135 32 1025 32 2019 32 42
Cuts at: 4722 4754 1373
Size: 32 2626
Fragments arranged by size:
2626 2019 1025 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3351 3597 669
Size: 988 281 1413 246 3079
Fragments arranged by size:
3079 1413 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 2894 3332 2894
Size: 438 5569
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3095 3113 5121 5305 1305
Size: 1790 18 2008 184 2007
Fragments arranged by size:
2008 2007 1790 184 18
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4185
Fragments arranged by size:
4185 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2073 2912 3965 4324 4483 518
Size: 648 907 839 1053 359 159 2042
Fragments arranged by size:
2042 1053 907 839 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3423 4100 5248 5493 1606
Size: 1817 677 1148 245 2120
Fragments arranged by size:
2120 1817 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5455
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6007
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 3365 4910 1132
Size: 97 2136 1545 2229
Fragments arranged by size:
2229 2136 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6007
PciI A'CATG_T
Cuts at: 4906 4906
Size: 6007
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3017 3017
Size: 6007
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 2208 3577 2208
Size: 1369 4638
PstI C_TGCA'G
Cuts at: 830 2872 3057 3093 3359 830
Size: 2042 185 36 266 3478
Fragments arranged by size:
3478 2042 266 185 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SbfI CC_TGCA'GG
Cuts at: 3057 3359 3057
Size: 302 5705
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2868 3053 3089 3355 4490 5171 5362
Size: 219 1823 185 36 266 1135 681 191
Cuts at: 5362 826
Size: 1471
Fragments arranged by size:
1823 1471 1135 681 266 219 191 185
36
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2458 4111 5012 5274 820
Size: 197 926 515 1653 901 262 1553
Fragments arranged by size:
1653 1553 926 901 515 262 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 3365 1132
Size: 2233 3774
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 2419 1517
Size: 902 5105
StyI C'CwwG_G
Cuts at: 513 1065 3446 513
Size: 552 2381 3074
Fragments arranged by size:
3074 2381 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4034 4051 4204 4389 4834 325
Size: 1255 2454 17 153 185 445 1498
Fragments arranged by size:
2454 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3262 4253 5965
Size: 274 80 33 51 1443 1285 991 1712
Cuts at: 5965 96
Size: 138
Fragments arranged by size:
1712 1443 1285 991 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5967 5999 5967
Size: 32 5975
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3156 3156
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6007
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 2037 2254 4136 2037
Size: 217 1882 3908
Fragments arranged by size:
3908 1882 217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI