Restriction map for
pFN21AE1921
(Circular) (Six-base) MAPSORT of: pFN21AE1921.seq Check: 3857 from: 1 to: 6076
pFN21AE1921 6076 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2225 3234 2225
Size: 1009 5067
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3886 278
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3419 1937
Size: 1482 4594
Acc65I G'GTAC_C
Cuts at: 2797 3397 2797
Size: 600 5476
AclI AA'CG_TT
Cuts at: 1563 4203 4576 1563
Size: 2640 373 3063
Fragments arranged by size:
3063 2640 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2948 3123 4139 5523 5791 1632
Size: 1316 175 1016 1384 268 1917
Fragments arranged by size:
1917 1384 1316 1016 268 175
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5879
AflIII A'CryG_T
Cuts at: 1203 4975 5952 1203
Size: 3772 977 1327
Fragments arranged by size:
3772 1327 977
AgeI A'CCGG_T
Cuts at: 4907 4907
Size: 6076
AhdI GACnn_n'nnGTC
Cuts at: 4805 4805
Size: 6076
AleI CACnn'nnGTG
Cuts at: 1166 2816 2884 1166
Size: 1650 68 4358
Fragments arranged by size:
4358 1650 68
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6076
AlwNI CAG_nnn'CTG
Cuts at: 2155 2971 3819 5391 2155
Size: 816 848 1572 2840
Fragments arranged by size:
2840 1572 848 816
ApaI G_GGCC'C
Cuts at: 2071 2903 2071
Size: 832 5244
ApaLI G'TGCA_C
Cuts at: 4133 5289 4133
Size: 1156 4920
ApoI r'AATT_y
Cuts at: 1493 3385 3611 3763 5872 1493
Size: 1892 226 152 2109 1697
Fragments arranged by size:
2109 1892 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4630 160
Size: 872 3598 1606
Fragments arranged by size:
3598 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
AvaI C'yCGr_G
Cuts at: 1870 1943 2235 3401 1870
Size: 73 292 1166 4545
Fragments arranged by size:
4545 1166 292 73
BamHI G'GATC_C
Cuts at: 2835 3406 3773 2835
Size: 571 367 5138
Fragments arranged by size:
5138 571 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2797 3222 3261 3284
Size: 325 217 627 267 743 425 39 23
Cuts at: 3284 3332 3397 4852 618
Size: 48 65 1455 1842
Fragments arranged by size:
1842 1455 743 627 425 325 267 217
65 48 39 23
BanII G_rGCy'C
Cuts at: 1391 2071 2639 2903 3126 3395 1391
Size: 680 568 264 223 269 4072
Fragments arranged by size:
4072 680 568 269 264 223
BbeI G_GCGC'C
Cuts at: 2058 3288 2058
Size: 1230 4846
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2438 2572 928
Size: 971 539 134 4432
Fragments arranged by size:
4432 971 539 134
BbvCI CC'TCA_GC
Cuts at: 3200 3200
Size: 6076
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4267 4301 1903
Size: 34 50 34 2246 34 3678
Fragments arranged by size:
3678 2246 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3968 5178 3968
Size: 1210 4866
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6076
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4687 136
Size: 107 122 71 631 605 3015 1525
Fragments arranged by size:
3015 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6071 6071
Size: 6076
BlpI GC'TnA_GC
Cuts at: 3493 3493
Size: 6076
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2903 3337 4137 5293 1790
Size: 281 832 434 800 1156 2573
Fragments arranged by size:
2573 1156 832 800 434 281
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6076
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3807 4765 442
Size: 416 53 83 399 24 2390 958 1753
Fragments arranged by size:
2390 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3023 1056
Size: 1967 4109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2177 2192 2363 3331 4736 1239
Size: 938 15 171 968 1405 2579
Fragments arranged by size:
2579 1405 968 938 171 15
Bpu10I CC'TnA_GC
Cuts at: 2495 3200 2495
Size: 705 5371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2998 4201 5066 5364 2998
Size: 1203 865 298 3710
Fragments arranged by size:
3710 1203 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4739 882
Size: 617 3240 2219
Fragments arranged by size:
3240 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3291 3867 493
Size: 711 2087 576 2702
Fragments arranged by size:
2702 2087 711 576
BsaBI GATnn'nnATC
Cuts at: 6070 6070
Size: 6076
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3234 3285 3883
Size: 53 83 186 936 522 1179 51 598
Cuts at: 3883 4265 4900 275
Size: 382 635 1451
Fragments arranged by size:
1451 1179 936 635 598 522 382 186
83 53 51
BsaWI w'CCGG_w
Cuts at: 3085 4509 4907 5181 5328 5739 3085
Size: 1424 398 274 147 411 3422
Fragments arranged by size:
3422 1424 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2612 2642 1178
Size: 30 165 30 1209 30 4612
Fragments arranged by size:
4612 1209 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2174 2275 2866 3334 1186
Size: 937 51 101 591 468 3928
Fragments arranged by size:
3928 937 591 468 101 51
BseYI C'CCAG_C
Cuts at: 2166 2790 2801 2859 5279 5775 2166
Size: 624 11 58 2420 496 2467
Fragments arranged by size:
2467 2420 624 496 58 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2159 2302 2503 3209 3313 1163
Size: 573 408 15 143 201 706 104 3926
Fragments arranged by size:
3926 706 573 408 201 143 104 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3526 4287 5315 1540
Size: 275 186 1525 761 1028 2301
Fragments arranged by size:
2301 1525 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2630 3126 4137 4222 5293 2630
Size: 496 1011 85 1071 3413
Fragments arranged by size:
3413 1071 1011 496 85
BsmI GAATG_Cn'
Cuts at: 3587 3680 3587
Size: 93 5983
BsmBI CGTCTCn'nnnn_
Cuts at: 2746 2746
Size: 6076
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2630 2639 2903 3126 3337 3395
Size: 399 281 559 9 264 223 211 58
Cuts at: 3395 4137 4222 5293 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 559 399 281 264 223
211 85 58 9
BspEI T'CCGG_A
Cuts at: 3085 5739 3085
Size: 2654 3422
BspHI T'CATG_A
Cuts at: 3965 5695 3965
Size: 1730 4346
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2225 3234 3292 3431 844
Size: 1381 1009 58 139 3489
Fragments arranged by size:
3489 1381 1009 139 58
BsrBI CCG'CTC
Cuts at: 1394 1823 2758 3342 3963 1394
Size: 429 935 584 621 3507
Fragments arranged by size:
3507 935 621 584 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2308 2361 4571 4745 65
Size: 1183 1060 53 2210 174 1396
Fragments arranged by size:
2210 1396 1183 1060 174 53
BsrFI r'CCGG_y
Cuts at: 1700 1810 2077 2435 3258 4720 4907 1700
Size: 110 267 358 823 1462 187 2869
Fragments arranged by size:
2869 1462 823 358 267 187 110
BsrGI T'GTAC_A
Cuts at: 96 2724 96
Size: 2628 3448
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6076
BssSI C'ACGA_G
Cuts at: 4136 5148 4136
Size: 1012 5064
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6076
BstBI TT'CG_AA
Cuts at: 4880 4880
Size: 6076
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6076
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 6076
BstYI r'GATC_y
Cuts at: 1972 2835 3406 3773 4158 4175 5627 5713 5725
Size: 863 571 367 385 17 1452 86 12
Cuts at: 5725 6071 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 863 571 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2564 2564
Size: 6076
BtgI C'CryG_G
Cuts at: 513 1065 2658 2814 513
Size: 552 1593 156 3775
Fragments arranged by size:
3775 1593 552 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2812 487
Size: 683 56 1586 3751
Fragments arranged by size:
3751 1586 683 56
BtsI GCAGTG_nn'
Cuts at: 2156 2515 3592 4386 4406 2156
Size: 359 1077 794 20 3826
Fragments arranged by size:
3826 1077 794 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6076
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6041
DraI TTT'AAA
Cuts at: 3380 3380
Size: 6076
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5083 809
Size: 507 3767 1802
Fragments arranged by size:
3767 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2086 2094 2222
Size: 54 1425 50 275 18 256 8 128
Cuts at: 2222 3523 4412 6041 8
Size: 1301 889 1629 43
Fragments arranged by size:
1629 1425 1301 889 275 256 128 54
50 43 18 8
EagI C'GGCC_G
Cuts at: 1537 1812 3523 1537
Size: 275 1711 4090
Fragments arranged by size:
4090 1711 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2552 2606 2987 4006 1353
Size: 932 267 54 381 1019 3423
Fragments arranged by size:
3423 1019 932 381 267 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3209 3220 4663 5037 5183 387
Size: 1064 138 1620 11 1443 374 146 1280
Fragments arranged by size:
1620 1443 1280 1064 374 146 138 11
EcoICRI GAG'CTC
Cuts at: 3124 3124
Size: 6076
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2177 2192 2363 2948 3123 3331 4139
Size: 393 545 15 171 585 175 208 808
Cuts at: 4139 4736 5523 5791 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 808 787 597 585 545 393 268
208 175 171 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2159 2159
Size: 6076
EcoO109I rG'GnC_Cy
Cuts at: 2068 2713 2899 2900 2068
Size: 645 186 1 5244
Fragments arranged by size:
5244 645 186 1
EcoRI G'AATT_C
Cuts at: 3385 3763 3385
Size: 378 5698
EcoRV GAT'ATC
Cuts at: 2986 2986
Size: 6076
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 6076
FspI TGC'GCA
Cuts at: 4582 4582
Size: 6076
HaeII r_GCGC'y
Cuts at: 1678 2058 3220 3288 5223 1678
Size: 380 1162 68 1935 2531
Fragments arranged by size:
2531 1935 1162 380 68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2612 2644 4717 4749 4791
Size: 32 135 32 1040 32 2073 32 42
Cuts at: 4791 4823 1373
Size: 32 2626
Fragments arranged by size:
2626 2073 1040 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3420 3666 669
Size: 988 281 1482 246 3079
Fragments arranged by size:
3079 1482 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6076
HpaI GTT'AAC
Cuts at: 3666 3666
Size: 6076
KasI G'GCGC_C
Cuts at: 2054 3284 2054
Size: 1230 4846
KpnI G_GTAC'C
Cuts at: 2801 3401 2801
Size: 600 5476
MfeI C'AATT_G
Cuts at: 3675 3675
Size: 6076
MluI A'CGCG_T
Cuts at: 5952 5952
Size: 6076
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2598 5190 5374 1305
Size: 1293 2592 184 2007
Fragments arranged by size:
2592 2007 1293 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2224 10
Size: 54 1425 343 392 3862
Fragments arranged by size:
3862 1425 392 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2537 2816 2884 4034 4393 4552 518
Size: 648 1371 279 68 1150 359 159 2042
Fragments arranged by size:
2042 1371 1150 648 359 279 159 68
MspA1I CmG'CkG
Cuts at: 1606 2023 2034 2170 2316 2576 2610 3492 4169
Size: 417 11 136 146 260 34 882 677
Cuts at: 4169 5317 5562 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 882 677 417 260 245 146
136 34 11
NaeI GCC'GGC
Cuts at: 2079 2079
Size: 6076
NarI GG'CG_CC
Cuts at: 2055 3285 2055
Size: 1230 4846
NcoI C'CATG_G
Cuts at: 513 1065 2658 2814 513
Size: 552 1593 156 3775
Fragments arranged by size:
3775 1593 552 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6076
NgoMIV G'CCGG_C
Cuts at: 2077 2077
Size: 6076
NheI G'CTAG_C
Cuts at: 1052 3019 1052
Size: 1967 4109
NotI GC'GGCC_GC
Cuts at: 3523 3523
Size: 6076
NspI r_CATG'y
Cuts at: 1132 1229 2542 3434 4979 1132
Size: 97 1313 892 1545 2229
Fragments arranged by size:
2229 1545 1313 892 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6076
PciI A'CATG_T
Cuts at: 4975 4975
Size: 6076
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2820 1108
Size: 1712 4364
PmeI GTTT'AAAC
Cuts at: 3380 3380
Size: 6076
PmlI CAC'GTG
Cuts at: 3291 3291
Size: 6076
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4145 4177 5658 5690 4145
Size: 32 1481 32 4531
Fragments arranged by size:
4531 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3003 3320 1613
Size: 1390 317 4369
Fragments arranged by size:
4369 1390 317
PsiI TTA'TAA
Cuts at: 3646 3646
Size: 6076
PspOMI G'GGCC_C
Cuts at: 2067 2899 2067
Size: 832 5244
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 6044
PstI C_TGCA'G
Cuts at: 830 2106 2195 3231 3428 830
Size: 1276 89 1036 197 3478
Fragments arranged by size:
3478 1276 1036 197 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6076
PvuII CAG'CTG
Cuts at: 2170 2316 2170
Size: 146 5930
SacI G_AGCT'C
Cuts at: 3126 3126
Size: 6076
SalI G'TCGA_C
Cuts at: 1936 3418 1936
Size: 1482 4594
SapI GCTCTTCn'nnn_
Cuts at: 2285 2285
Size: 6076
SbfI CC_TGCA'GG
Cuts at: 3231 3428 3231
Size: 197 5879
ScaI AGT'ACT
Cuts at: 2765 4324 6036 2765
Size: 1559 1712 2805
Fragments arranged by size:
2805 1712 1559
SfcI C'TryA_G
Cuts at: 826 1045 2102 2191 3227 3424 4559 5240 5431
Size: 219 1057 89 1036 197 1135 681 191
Cuts at: 5431 826
Size: 1471
Fragments arranged by size:
1471 1135 1057 1036 681 219 197 191
89
SfoI GGC'GCC
Cuts at: 2056 3286 2056
Size: 1230 4846
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6076
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6076
SmaI CCC'GGG
Cuts at: 1872 3403 1872
Size: 1531 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3013 4180 5081 5343 820
Size: 197 926 1070 1167 901 262 1553
Fragments arranged by size:
1553 1167 1070 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3867 493
Size: 3374 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6076
SphI G_CATG'C
Cuts at: 1132 3434 1132
Size: 2302 3774
SspI AAT'ATT
Cuts at: 5 52 2675 4000 5
Size: 47 2623 1325 2081
Fragments arranged by size:
2623 2081 1325 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6076
StyI C'CwwG_G
Cuts at: 513 1065 2071 2658 2814 3515 513
Size: 552 1006 587 156 701 3074
Fragments arranged by size:
3074 1006 701 587 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2844 4103 4120 4273 4458 4903 325
Size: 1255 1264 1259 17 153 185 445 1498
Fragments arranged by size:
1498 1264 1259 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2724 2763 3058
Size: 274 80 33 51 1443 747 39 295
Cuts at: 3058 4322 6034 96
Size: 1264 1712 138
Fragments arranged by size:
1712 1443 1264 747 295 274 138 80
51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2840 2872 6036 6068 2840
Size: 32 3164 32 2848
Fragments arranged by size:
3164 2848 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6076
XbaI T'CTAG_A
Cuts at: 3412 3412
Size: 6076
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6076
XmaI C'CCGG_G
Cuts at: 1870 3401 1870
Size: 1531 4545
XmnI GAAnn'nnTTC
Cuts at: 2765 4205 2765
Size: 1440 4636
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3884 276
Size: 53 83 186 936 2350 2468
Fragments arranged by size:
2468 2350 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII FseI FspAI NruI NsiI PacI PfoI
PpuMI PspXI RsrII SacII SanDI SexAI SfiI SrfI
SwaI XcmI