Restriction map for
pFN21AE1920
(Circular) (Six-base) MAPSORT of: pFN21AE1920.seq Check: 9139 from: 1 to: 6070
pFN21AE1920 6070 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3880 278
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3413 1937
Size: 1476 4594
Acc65I G'GTAC_C
Cuts at: 3391 3391
Size: 6070
AclI AA'CG_TT
Cuts at: 1563 4197 4570 1563
Size: 2634 373 3063
Fragments arranged by size:
3063 2634 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 2765 2961 4133 5517 5785 1632
Size: 407 726 196 1172 1384 268 1917
Fragments arranged by size:
1917 1384 1172 726 407 268 196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5873
AflIII A'CryG_T
Cuts at: 1203 4969 5946 1203
Size: 3766 977 1327
Fragments arranged by size:
3766 1327 977
AgeI A'CCGG_T
Cuts at: 4901 4901
Size: 6070
AhdI GACnn_n'nnGTC
Cuts at: 4799 4799
Size: 6070
AleI CACnn'nnGTG
Cuts at: 1166 2384 2846 1166
Size: 1218 462 4390
Fragments arranged by size:
4390 1218 462
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6070
AlwNI CAG_nnn'CTG
Cuts at: 2107 2431 3813 5385 2107
Size: 324 1382 1572 2792
Fragments arranged by size:
2792 1572 1382 324
ApaI G_GGCC'C
Cuts at: 3104 3104
Size: 6070
ApaLI G'TGCA_C
Cuts at: 4127 5283 4127
Size: 1156 4914
ApoI r'AATT_y
Cuts at: 1493 3379 3605 3757 5866 1493
Size: 1886 226 152 2109 1697
Fragments arranged by size:
2109 1886 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4624 160
Size: 872 3592 1606
Fragments arranged by size:
3592 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 2669 2808 3395 1870
Size: 73 121 605 139 587 4545
Fragments arranged by size:
4545 605 587 139 121 73
BamHI G'GATC_C
Cuts at: 2128 2136 3400 3767 2128
Size: 8 1264 367 4431
Fragments arranged by size:
4431 1264 367 8
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3391 4846 618
Size: 325 217 627 1604 1455 1842
Fragments arranged by size:
1842 1604 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3104 3389 1391
Size: 1713 285 4072
Fragments arranged by size:
4072 1713 285
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 928
Size: 971 399 4700
Fragments arranged by size:
4700 971 399
BbvCI CC'TCA_GC
Cuts at: 2829 3215 2829
Size: 386 5684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3096 3130 4261 4295 1903
Size: 34 1159 34 1131 34 3678
Fragments arranged by size:
3678 1159 1131 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3962 5172 3962
Size: 1210 4860
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6070
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2903 4681 136
Size: 107 122 71 631 605 1231 1778 1525
Fragments arranged by size:
1778 1525 1231 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6065 6065
Size: 6070
BlpI GC'TnA_GC
Cuts at: 2337 3487 2337
Size: 1150 4920
Bme1580I G_kGCm'C
Cuts at: 1790 3104 4131 5287 1790
Size: 1314 1027 1156 2573
Fragments arranged by size:
2573 1314 1156 1027
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6070
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2317 2953 3086
Size: 416 53 83 399 24 900 636 133
Cuts at: 3086 3801 4759 442
Size: 715 958 1753
Fragments arranged by size:
1753 958 900 715 636 416 399 133
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6070
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2352 2428 2779 2352
Size: 76 351 5643
Fragments arranged by size:
5643 351 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2129 2171 2456 2615 2774 2776 4730 1239
Size: 890 42 285 159 159 2 1954 2579
Fragments arranged by size:
2579 1954 890 285 159 159 42 2
Bpu10I CC'TnA_GC
Cuts at: 2829 2874 3215 2829
Size: 45 341 5684
Fragments arranged by size:
5684 341 45
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2222 2591 2630 4195 5060 5358 2222
Size: 369 39 1565 865 298 2934
Fragments arranged by size:
2934 1565 865 369 298 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2399 4733 882
Size: 617 900 2334 2219
Fragments arranged by size:
2334 2219 900 617
BsaAI yAC'GTr
Cuts at: 493 1204 3861 493
Size: 711 2657 2702
Fragments arranged by size:
2702 2657 711
BsaBI GATnn'nnATC
Cuts at: 6064 6064
Size: 6070
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3877 4259 4894 275
Size: 53 83 186 936 2344 382 635 1451
Fragments arranged by size:
2344 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3291 4503 4901 5175 5322 5733 3291
Size: 1212 398 274 147 411 3628
Fragments arranged by size:
3628 1212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5845
Fragments arranged by size:
5845 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2093 2096 2126 3118 3168 1186
Size: 865 42 3 30 992 50 4088
Fragments arranged by size:
4088 992 865 50 42 30 3
BseYI C'CCAG_C
Cuts at: 3053 5273 5769 3053
Size: 2220 496 3354
Fragments arranged by size:
3354 2220 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2684 3380 1163
Size: 573 948 696 3853
Fragments arranged by size:
3853 948 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3520 4281 5309 1540
Size: 275 186 1519 761 1028 2301
Fragments arranged by size:
2301 1519 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 3320 4131 4216 5287 2391
Size: 929 811 85 1071 3174
Fragments arranged by size:
3174 1071 929 811 85
BsmI GAATG_Cn'
Cuts at: 3119 3581 3674 3119
Size: 462 93 5515
Fragments arranged by size:
5515 462 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2543 2543
Size: 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 3104 3320 3389 4131 4216 5287
Size: 399 601 713 216 69 742 85 1071
Cuts at: 5287 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 713 601 399 216 85
69
BspEI T'CCGG_A
Cuts at: 5733 5733
Size: 6070
BspHI T'CATG_A
Cuts at: 3959 5689 3959
Size: 1730 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2706 3425 844
Size: 1862 719 3489
Fragments arranged by size:
3489 1862 719
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 2226 2782 3957 1394
Size: 429 208 195 556 1175 3507
Fragments arranged by size:
3507 1175 556 429 208 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 4565 4739 65
Size: 1183 3317 174 1396
Fragments arranged by size:
3317 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4714 4901 1700
Size: 110 2904 187 2869
Fragments arranged by size:
2904 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6070
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6070
BssSI C'ACGA_G
Cuts at: 2777 4130 5142 2777
Size: 1353 1012 3705
Fragments arranged by size:
3705 1353 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2431 2476 794
Size: 1637 45 4388
Fragments arranged by size:
4388 1637 45
BstBI TT'CG_AA
Cuts at: 4874 4874
Size: 6070
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6070
BstXI CCAn_nnnn'nTGG
Cuts at: 2416 2800 2893 2416
Size: 384 93 5593
Fragments arranged by size:
5593 384 93
BstYI r'GATC_y
Cuts at: 1972 2128 2136 2772 2854 3046 3400 3767 4152
Size: 156 8 636 82 192 354 367 385
Cuts at: 4152 4169 5621 5707 5719 6065 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 636 385 367 354 346 192
156 86 82 17 12 8
Bsu36I CC'TnA_GG
Cuts at: 3270 3270
Size: 6070
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2574 2799 513
Size: 552 939 165 405 225 3784
Fragments arranged by size:
3784 939 552 405 225 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5331
Fragments arranged by size:
5331 683 56
BtsI GCAGTG_nn'
Cuts at: 3232 3238 3586 4380 4400 3232
Size: 6 348 794 20 4902
Fragments arranged by size:
4902 794 348 20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6035
DraI TTT'AAA
Cuts at: 3374 3374
Size: 6070
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5077 809
Size: 507 3761 1802
Fragments arranged by size:
3761 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2418 3517 4406
Size: 54 1425 50 275 18 588 1099 889
Cuts at: 4406 6035 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1099 889 588 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3517 1537
Size: 275 1705 4090
Fragments arranged by size:
4090 1705 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 2082 2115 3322 4000 1353
Size: 717 12 33 1207 678 3423
Fragments arranged by size:
3423 1207 717 678 33 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2452 2525 4657 5031 5177 387
Size: 1064 138 863 73 2132 374 146 1280
Fragments arranged by size:
2132 1280 1064 863 374 146 138 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2129 2171 2456 2615 2765 2774
Size: 393 407 90 42 285 159 150 9
Cuts at: 2774 2776 2961 4133 4730 5517 5785 1239
Size: 2 185 1172 597 787 268 1524
Fragments arranged by size:
1524 1172 787 597 407 393 285 268
185 159 150 90 42 9 2
EcoO109I rG'GnC_Cy
Cuts at: 2804 3101 3123 3211 2804
Size: 297 22 88 5663
Fragments arranged by size:
5663 297 88 22
EcoRI G'AATT_C
Cuts at: 3379 3757 3379
Size: 378 5692
EcoRV GAT'ATC
Cuts at: 2179 2179
Size: 6070
FspI TGC'GCA
Cuts at: 4576 4576
Size: 6070
HaeII r_GCGC'y
Cuts at: 1678 2791 5217 1678
Size: 1113 2426 2531
Fragments arranged by size:
2531 2426 1113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2467 2499 4711 4743 4785
Size: 32 135 32 895 32 2212 32 42
Cuts at: 4785 4817 1373
Size: 32 2626
Fragments arranged by size:
2626 2212 895 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3414 3660 669
Size: 988 281 1476 246 3079
Fragments arranged by size:
3079 1476 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6070
HpaI GTT'AAC
Cuts at: 3660 3660
Size: 6070
KpnI G_GTAC'C
Cuts at: 3395 3395
Size: 6070
MfeI C'AATT_G
Cuts at: 3669 3669
Size: 6070
MluI A'CGCG_T
Cuts at: 5946 5946
Size: 6070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5184 5368 1305
Size: 3879 184 2007
Fragments arranged by size:
3879 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4248
Fragments arranged by size:
4248 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2384 2846 2891 3353 4028 4387 4546
Size: 648 1218 462 45 462 675 359 159
Cuts at: 4546 518
Size: 2042
Fragments arranged by size:
2042 1218 675 648 462 462 359 159
45
MspA1I CmG'CkG
Cuts at: 1606 2341 2428 2593 2922 3120 3486 4163 5311
Size: 735 87 165 329 198 366 677 1148
Cuts at: 5311 5556 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 735 677 366 329 245 198
165 87
NcoI C'CATG_G
Cuts at: 513 1065 2004 2574 2799 513
Size: 552 939 570 225 3784
Fragments arranged by size:
3784 939 570 552 225
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6070
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6070
NotI GC'GGCC_GC
Cuts at: 3517 3517
Size: 6070
NspI r_CATG'y
Cuts at: 1132 1229 2587 3428 4973 1132
Size: 97 1358 841 1545 2229
Fragments arranged by size:
2229 1545 1358 841 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6070
PciI A'CATG_T
Cuts at: 4969 4969
Size: 6070
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3283 1108
Size: 2175 3895
PmeI GTTT'AAAC
Cuts at: 3374 3374
Size: 6070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4139 4171 5652 5684 4139
Size: 32 1481 32 4525
Fragments arranged by size:
4525 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6070
PsiI TTA'TAA
Cuts at: 3640 3640
Size: 6070
PspOMI G'GGCC_C
Cuts at: 3100 3100
Size: 6070
PspXI vC'TCGA_Gb
Cuts at: 2669 2669
Size: 6070
PstI C_TGCA'G
Cuts at: 830 2703 3422 830
Size: 1873 719 3478
Fragments arranged by size:
3478 1873 719
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6070
PvuII CAG'CTG
Cuts at: 2341 2593 2922 2341
Size: 252 329 5489
Fragments arranged by size:
5489 329 252
SalI G'TCGA_C
Cuts at: 1936 3412 1936
Size: 1476 4594
SbfI CC_TGCA'GG
Cuts at: 2703 3422 2703
Size: 719 5351
ScaI AGT'ACT
Cuts at: 2657 4318 6030 2657
Size: 1661 1712 2697
Fragments arranged by size:
2697 1712 1661
SfcI C'TryA_G
Cuts at: 826 1045 2699 2923 3311 3418 4553 5234 5425
Size: 219 1654 224 388 107 1135 681 191
Cuts at: 5425 826
Size: 1471
Fragments arranged by size:
1654 1471 1135 681 388 224 219 191
107
SfiI GGCCn_nnn'nGGCC
Cuts at: 2903 2903
Size: 6070
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6070
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6070
SmaI CCC'GGG
Cuts at: 1872 2066 3397 1872
Size: 194 1331 4545
Fragments arranged by size:
4545 1331 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2201 2606 2645 2669 4174 5075
Size: 197 926 258 405 39 24 1505 901
Cuts at: 5075 5337 820
Size: 262 1553
Fragments arranged by size:
1553 1505 926 901 405 262 258 197
39 24
SnaBI TAC'GTA
Cuts at: 493 3861 493
Size: 3368 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6070
SphI G_CATG'C
Cuts at: 1132 2587 3428 1132
Size: 1455 841 3774
Fragments arranged by size:
3774 1455 841
SspI AAT'ATT
Cuts at: 5 52 3994 5
Size: 47 3942 2081
Fragments arranged by size:
3942 2081 47
StuI AGG'CCT
Cuts at: 1517 2906 3034 3281 1517
Size: 1389 128 247 4306
Fragments arranged by size:
4306 1389 247 128
StyI C'CwwG_G
Cuts at: 513 1065 2004 2400 2574 2799 3282 3509 513
Size: 552 939 396 174 225 483 227 3074
Fragments arranged by size:
3074 939 552 483 396 227 225 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4097 4114 4267 4452 4897 325
Size: 1255 2517 17 153 185 445 1498
Fragments arranged by size:
2517 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2655 2730 4316
Size: 274 80 33 51 1443 678 75 1586
Cuts at: 4316 6028 96
Size: 1712 138
Fragments arranged by size:
1712 1586 1443 678 274 138 80 75
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6030 6062 6030
Size: 32 6038
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6070
XbaI T'CTAG_A
Cuts at: 3406 3406
Size: 6070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 2713 2533
Size: 180 5890
XhoI C'TCGA_G
Cuts at: 1943 2669 1943
Size: 726 5344
XmaI C'CCGG_G
Cuts at: 1870 2064 3395 1870
Size: 194 1331 4545
Fragments arranged by size:
4545 1331 194
XmnI GAAnn'nnTTC
Cuts at: 4199 4199
Size: 6070
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3878 276
Size: 53 83 186 936 2344 2468
Fragments arranged by size:
2468 2344 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI
SexAI SfoI SrfI SwaI