Restriction map for
pFN21AE1916
(Circular) (Six-base) MAPSORT of: pFN21AE1916.seq Check: 1073 from: 1 to: 6064
pFN21AE1916 6064 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3874 278
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2360 2751 3407 1937
Size: 423 391 656 4594
Fragments arranged by size:
4594 656 423 391
Acc65I G'GTAC_C
Cuts at: 3385 3385
Size: 6064
AclI AA'CG_TT
Cuts at: 1563 3363 4191 4564 1563
Size: 1800 828 373 3063
Fragments arranged by size:
3063 1800 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2859 3103 4127 5511 5779 1632
Size: 1227 244 1024 1384 268 1917
Fragments arranged by size:
1917 1384 1227 1024 268 244
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5867
AflIII A'CryG_T
Cuts at: 1203 2742 4963 5940 1203
Size: 1539 2221 977 1327
Fragments arranged by size:
2221 1539 1327 977
AgeI A'CCGG_T
Cuts at: 4895 4895
Size: 6064
AhdI GACnn_n'nnGTC
Cuts at: 2938 4793 2938
Size: 1855 4209
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6064
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6064
AlwNI CAG_nnn'CTG
Cuts at: 3807 5379 3807
Size: 1572 4492
ApaLI G'TGCA_C
Cuts at: 4121 5277 4121
Size: 1156 4908
ApoI r'AATT_y
Cuts at: 1493 2532 2563 2897 3373 3599 3751 5860 1493
Size: 1039 31 334 476 226 152 2109 1697
Fragments arranged by size:
2109 1697 1039 476 334 226 152 31
AseI AT'TA_AT
Cuts at: 160 1032 2310 2622 4618 160
Size: 872 1278 312 1996 1606
Fragments arranged by size:
1996 1606 1278 872 312
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
AvaI C'yCGr_G
Cuts at: 1870 1943 3166 3389 1870
Size: 73 1223 223 4545
Fragments arranged by size:
4545 1223 223 73
BamHI G'GATC_C
Cuts at: 3394 3761 3394
Size: 367 5697
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3294 3385 4840 618
Size: 325 217 627 1507 91 1455 1842
Fragments arranged by size:
1842 1507 1455 627 325 217 91
BanII G_rGCy'C
Cuts at: 1391 3383 1391
Size: 1992 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2452 928
Size: 971 553 4540
Fragments arranged by size:
4540 971 553
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2728 2762 4255 4289 1903
Size: 34 791 34 1493 34 3678
Fragments arranged by size:
3678 1493 791 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3956 5166 3956
Size: 1210 4854
BclI T'GATC_A
Cuts at: 1548 2584 1548
Size: 1036 5028
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2037 4675 136
Size: 107 122 71 631 605 365 2638 1525
Fragments arranged by size:
2638 1525 631 605 365 122 107 71
BglII A'GATC_T
Cuts at: 2143 6059 2143
Size: 3916 2148
BlpI GC'TnA_GC
Cuts at: 3481 3481
Size: 6064
Bme1580I G_kGCm'C
Cuts at: 1790 4125 5281 1790
Size: 2335 1156 2573
Fragments arranged by size:
2573 2335 1156
BmgBI CAC'GTC
Cuts at: 1318 2095 1318
Size: 777 5287
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3795 4753 442
Size: 416 53 83 399 24 2378 958 1753
Fragments arranged by size:
2378 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2160 3179 4724 1239
Size: 921 1019 1545 2579
Fragments arranged by size:
2579 1545 1019 921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4189 5054 5352 4189
Size: 865 298 4901
Fragments arranged by size:
4901 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2448 2978 4727 882
Size: 617 949 530 1749 2219
Fragments arranged by size:
2219 1749 949 617 530
BsaAI yAC'GTr
Cuts at: 493 1204 3855 493
Size: 711 2651 2702
Fragments arranged by size:
2702 2651 711
BsaBI GATnn'nnATC
Cuts at: 6058 6058
Size: 6064
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3871 4253 4888 275
Size: 53 83 186 936 2338 382 635 1451
Fragments arranged by size:
2338 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2266 4497 4895 5169 5316 5727 2266
Size: 2231 398 274 147 411 2603
Fragments arranged by size:
2603 2231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2777 2801 2807 2831 1178
Size: 30 165 30 1374 24 6 24 4411
Fragments arranged by size:
4411 1374 165 30 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 2785 1186
Size: 1519 80 4465
Fragments arranged by size:
4465 1519 80
BseYI C'CCAG_C
Cuts at: 2846 5267 5763 2846
Size: 2421 496 3147
Fragments arranged by size:
3147 2421 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3266 1163
Size: 573 1530 3961
Fragments arranged by size:
3961 1530 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3514 4275 5303 1540
Size: 275 186 1513 761 1028 2301
Fragments arranged by size:
2301 1513 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4125 4210 5281 4125
Size: 85 1071 4908
Fragments arranged by size:
4908 1071 85
BsmI GAATG_Cn'
Cuts at: 2344 3575 3668 2344
Size: 1231 93 4740
Fragments arranged by size:
4740 1231 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3383 4125 4210 5281 1391
Size: 399 1593 742 85 1071 2174
Fragments arranged by size:
2174 1593 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5727 5727
Size: 6064
BspHI T'CATG_A
Cuts at: 2260 2587 3093 3953 5683 2260
Size: 327 506 860 1730 2641
Fragments arranged by size:
2641 1730 860 506 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2059 3015 3419 844
Size: 1215 956 404 3489
Fragments arranged by size:
3489 1215 956 404
BsrBI CCG'CTC
Cuts at: 1394 1823 3951 1394
Size: 429 2128 3507
Fragments arranged by size:
3507 2128 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3312 4559 4733 65
Size: 1183 2064 1247 174 1396
Fragments arranged by size:
2064 1396 1247 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2977 4708 4895 1700
Size: 110 1167 1731 187 2869
Fragments arranged by size:
2869 1731 1167 187 110
BsrGI T'GTAC_A
Cuts at: 96 3339 96
Size: 3243 2821
BssHII G'CGCG_C
Cuts at: 1925 3187 1925
Size: 1262 4802
BssSI C'ACGA_G
Cuts at: 2999 4124 5136 2999
Size: 1125 1012 3927
Fragments arranged by size:
3927 1125 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3021 794
Size: 2227 3837
BstBI TT'CG_AA
Cuts at: 4868 4868
Size: 6064
BstEII G'GTnAC_C
Cuts at: 1184 2325 1184
Size: 1141 4923
BstYI r'GATC_y
Cuts at: 1972 2143 2503 2842 3276 3394 3761 4146 4163
Size: 171 360 339 434 118 367 385 17
Cuts at: 4163 5615 5701 5713 6059 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 434 385 367 360 346 339
171 118 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 2499 2525 3300 3318 513
Size: 552 939 30 465 26 775 18 3259
Fragments arranged by size:
3259 939 775 552 465 30 26 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5325
Fragments arranged by size:
5325 683 56
BtsI GCAGTG_nn'
Cuts at: 2709 3580 4374 4394 2709
Size: 871 794 20 4379
Fragments arranged by size:
4379 871 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6064
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6029
DraI TTT'AAA
Cuts at: 3368 3368
Size: 6064
DraIII CAC_nnn'GTG
Cuts at: 3301 3301
Size: 6064
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5071 809
Size: 507 3755 1802
Fragments arranged by size:
3755 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2097 3511 4400
Size: 54 1425 50 275 18 267 1414 889
Cuts at: 4400 6029 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1414 889 275 267 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3511 1537
Size: 275 1699 4090
Fragments arranged by size:
4090 1699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2826 3994 1353
Size: 1473 1168 3423
Fragments arranged by size:
3423 1473 1168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 4651 5025 5171 387
Size: 1064 138 1176 1886 374 146 1280
Fragments arranged by size:
1886 1280 1176 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2160 2859 3103 3179 4127 4724 5511
Size: 393 528 699 244 76 948 597 787
Cuts at: 5511 5779 1239
Size: 268 1524
Fragments arranged by size:
1524 948 787 699 597 528 393 268
244 76
EcoO109I rG'GnC_Cy
Cuts at: 2482 2856 2956 3102 2482
Size: 374 100 146 5444
Fragments arranged by size:
5444 374 146 100
EcoRI G'AATT_C
Cuts at: 3373 3751 3373
Size: 378 5686
FspI TGC'GCA
Cuts at: 3011 4570 3011
Size: 1559 4505
HaeII r_GCGC'y
Cuts at: 1678 2477 3090 5211 1678
Size: 799 613 2121 2531
Fragments arranged by size:
2531 2121 799 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2777 2801 2809 2833 2924
Size: 32 135 32 1205 24 8 24 91
Cuts at: 2924 2956 4705 4737 4779 4811 1373
Size: 32 1749 32 42 32 2626
Fragments arranged by size:
2626 1749 1205 135 91 42 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3408 3654 669
Size: 988 281 1470 246 3079
Fragments arranged by size:
3079 1470 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2653 748
Size: 1905 4159
HpaI GTT'AAC
Cuts at: 3654 3654
Size: 6064
KpnI G_GTAC'C
Cuts at: 3389 3389
Size: 6064
MfeI C'AATT_G
Cuts at: 3663 3663
Size: 6064
MluI A'CGCG_T
Cuts at: 5940 5940
Size: 6064
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5178 5362 1305
Size: 3873 184 2007
Fragments arranged by size:
3873 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4242
Fragments arranged by size:
4242 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3317 4022 4381 4540 518
Size: 648 2151 705 359 159 2042
Fragments arranged by size:
2151 2042 705 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2036 3480 4157 5305 5550 1606
Size: 430 1444 677 1148 245 2120
Fragments arranged by size:
2120 1444 1148 677 430 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2499 513
Size: 552 939 495 4078
Fragments arranged by size:
4078 939 552 495
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6064
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6064
NotI GC'GGCC_GC
Cuts at: 3511 3511
Size: 6064
NspI r_CATG'y
Cuts at: 1132 1229 2791 3422 4967 1132
Size: 97 1562 631 1545 2229
Fragments arranged by size:
2229 1562 1545 631 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6064
PciI A'CATG_T
Cuts at: 4963 4963
Size: 6064
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6064
PfoI T'CCnGG_A
Cuts at: 3348 3348
Size: 6064
PmeI GTTT'AAAC
Cuts at: 3368 3368
Size: 6064
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4133 4165 5646 5678 4133
Size: 32 1481 32 4519
Fragments arranged by size:
4519 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2482 2856 2956 3102 2482
Size: 374 100 146 5444
Fragments arranged by size:
5444 374 146 100
PshAI GACnn'nnGTC
Cuts at: 1613 2702 1613
Size: 1089 4975
PsiI TTA'TAA
Cuts at: 2424 3634 2424
Size: 1210 4854
PstI C_TGCA'G
Cuts at: 830 2608 2716 3416 830
Size: 1778 108 700 3478
Fragments arranged by size:
3478 1778 700 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6064
SacII CC_GC'GG
Cuts at: 2037 2037
Size: 6064
SalI G'TCGA_C
Cuts at: 1936 3406 1936
Size: 1470 4594
SanDI GG'GwC_CC
Cuts at: 2482 2482
Size: 6064
SapI GCTCTTCn'nnn_
Cuts at: 2826 2826
Size: 6064
SbfI CC_TGCA'GG
Cuts at: 3416 3416
Size: 6064
ScaI AGT'ACT
Cuts at: 4312 6024 4312
Size: 1712 4352
SexAI A'CCwGG_T
Cuts at: 2991 2991
Size: 6064
SfcI C'TryA_G
Cuts at: 826 1045 2604 2712 2752 3412 4547 5228 5419
Size: 219 1559 108 40 660 1135 681 191
Cuts at: 5419 826
Size: 1471
Fragments arranged by size:
1559 1471 1135 681 660 219 191 108
40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6064
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6064
SmaI CCC'GGG
Cuts at: 1872 3391 1872
Size: 1519 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3166 4168 5069 5331 820
Size: 197 926 1223 1002 901 262 1553
Fragments arranged by size:
1553 1223 1002 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3855 493
Size: 3362 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6064
SphI G_CATG'C
Cuts at: 1132 3422 1132
Size: 2290 3774
SspI AAT'ATT
Cuts at: 5 52 2490 3988 5
Size: 47 2438 1498 2081
Fragments arranged by size:
2438 2081 1498 47
StuI AGG'CCT
Cuts at: 1517 3164 1517
Size: 1647 4417
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 3503 513
Size: 552 939 495 1004 3074
Fragments arranged by size:
3074 1004 939 552 495
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2714 4091 4108 4261 4446 4891 325
Size: 1255 1134 1377 17 153 185 445 1498
Fragments arranged by size:
1498 1377 1255 1134 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3339 4310 6022
Size: 274 80 33 51 1443 1362 971 1712
Cuts at: 6022 96
Size: 138
Fragments arranged by size:
1712 1443 1362 971 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2380 2412 6024 6056 2380
Size: 32 3612 32 2388
Fragments arranged by size:
3612 2388 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3100 3308 1316
Size: 1784 208 4072
Fragments arranged by size:
4072 1784 208
XbaI T'CTAG_A
Cuts at: 2290 3400 2290
Size: 1110 4954
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 2506
Size: 6064
XhoI C'TCGA_G
Cuts at: 1943 3166 1943
Size: 1223 4841
XmaI C'CCGG_G
Cuts at: 1870 3389 1870
Size: 1519 4545
XmnI GAAnn'nnTTC
Cuts at: 4193 4193
Size: 6064
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3872 276
Size: 53 83 186 936 2338 2468
Fragments arranged by size:
2468 2338 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SfiI SfoI SrfI
SwaI