Restriction map for
pFN21AE1915
(Circular) (Six-base) MAPSORT of: pFN21AE1915.seq Check: 6481 from: 1 to: 6061
pFN21AE1915 6061 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2724 3871 278
Size: 53 83 186 936 1188 1147 2468
Fragments arranged by size:
2468 1188 1147 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2474 3404 1937
Size: 537 930 4594
Fragments arranged by size:
4594 930 537
Acc65I G'GTAC_C
Cuts at: 3382 3382
Size: 6061
AclI AA'CG_TT
Cuts at: 1563 4188 4561 1563
Size: 2625 373 3063
Fragments arranged by size:
3063 2625 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2510 4124 5508 5776 1632
Size: 731 147 1614 1384 268 1917
Fragments arranged by size:
1917 1614 1384 731 268 147
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5864
AflIII A'CryG_T
Cuts at: 1203 4960 5937 1203
Size: 3757 977 1327
Fragments arranged by size:
3757 1327 977
AgeI A'CCGG_T
Cuts at: 3285 4892 3285
Size: 1607 4454
AhdI GACnn_n'nnGTC
Cuts at: 4790 4790
Size: 6061
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2793 1184
Size: 1609 4452
AlwNI CAG_nnn'CTG
Cuts at: 2483 3804 5376 2483
Size: 1321 1572 3168
Fragments arranged by size:
3168 1572 1321
ApaLI G'TGCA_C
Cuts at: 2627 4118 5274 2627
Size: 1491 1156 3414
Fragments arranged by size:
3414 1491 1156
ApoI r'AATT_y
Cuts at: 1493 2549 2654 2773 3278 3370 3596 3748 5857
Size: 1056 105 119 505 92 226 152 2109
Cuts at: 5857 1493
Size: 1697
Fragments arranged by size:
2109 1697 1056 505 226 152 119 105
92
AseI AT'TA_AT
Cuts at: 160 1032 4615 160
Size: 872 3583 1606
Fragments arranged by size:
3583 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
AvaI C'yCGr_G
Cuts at: 1870 1943 3386 1870
Size: 73 1443 4545
Fragments arranged by size:
4545 1443 73
BamHI G'GATC_C
Cuts at: 3020 3391 3758 3020
Size: 371 367 5323
Fragments arranged by size:
5323 371 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 2846 3382 4837 618
Size: 325 217 627 224 835 536 1455 1842
Fragments arranged by size:
1842 1455 835 627 536 325 224 217
BanII G_rGCy'C
Cuts at: 1391 2223 3380 1391
Size: 832 1157 4072
Fragments arranged by size:
4072 1157 832
BbeI G_GCGC'C
Cuts at: 2015 2850 2015
Size: 835 5226
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5090
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2811 2845 3155 3189 4252 4286 1903
Size: 34 874 34 310 34 1063 34 3678
Fragments arranged by size:
3678 1063 874 310 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3953 5163 3953
Size: 1210 4851
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6061
BfrBI ATG'CAT
Cuts at: 2101 2101
Size: 6061
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4672 136
Size: 107 122 71 631 605 337 2663 1525
Fragments arranged by size:
2663 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6056 6056
Size: 6061
BlpI GC'TnA_GC
Cuts at: 3478 3478
Size: 6061
Bme1580I G_kGCm'C
Cuts at: 1790 2631 4122 5278 1790
Size: 841 1491 1156 2573
Fragments arranged by size:
2573 1491 1156 841
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6061
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3792 4750 442
Size: 416 53 83 399 24 2375 958 1753
Fragments arranged by size:
2375 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2147 1056
Size: 1091 4970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2366 2658 2685 2861 4721 1239
Size: 968 159 292 27 176 1860 2579
Fragments arranged by size:
2579 1860 968 292 176 159 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2437 3162 4186 5051 5349 2437
Size: 725 1024 865 298 3149
Fragments arranged by size:
3149 1024 865 725 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2321 4724 882
Size: 617 822 2403 2219
Fragments arranged by size:
2403 2219 822 617
BsaAI yAC'GTr
Cuts at: 493 1204 3211 3852 493
Size: 711 2007 641 2702
Fragments arranged by size:
2702 2007 711 641
BsaBI GATnn'nnATC
Cuts at: 6055 6055
Size: 6061
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2012 2721 2847 3868
Size: 53 83 186 936 479 709 126 1021
Cuts at: 3868 4250 4885 275
Size: 382 635 1451
Fragments arranged by size:
1451 1021 936 709 635 479 382 186
126 83 53
BsaWI w'CCGG_w
Cuts at: 2431 3285 4494 4892 5166 5313 5724 2431
Size: 854 1209 398 274 147 411 2768
Fragments arranged by size:
2768 1209 854 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2339 2369 2658 2688 1178
Size: 30 165 30 936 30 289 30 4551
Fragments arranged by size:
4551 936 289 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2816 3050 1186
Size: 1069 561 234 4197
Fragments arranged by size:
4197 1069 561 234
BseYI C'CCAG_C
Cuts at: 5264 5760 5264
Size: 496 5565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2730 2775 1163
Size: 573 994 45 4449
Fragments arranged by size:
4449 994 573 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3511 4272 5300 1540
Size: 275 186 1510 761 1028 2301
Fragments arranged by size:
2301 1510 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2223 2631 4122 4207 5278 2223
Size: 408 1491 85 1071 3006
Fragments arranged by size:
3006 1491 1071 408 85
BsmI GAATG_Cn'
Cuts at: 3572 3665 3572
Size: 93 5968
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2223 2631 3380 4122 4207 5278 1391
Size: 399 433 408 749 742 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 433 408 399 85
BspEI T'CCGG_A
Cuts at: 5724 5724
Size: 6061
BspHI T'CATG_A
Cuts at: 3950 5680 3950
Size: 1730 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2797 3416 844
Size: 1953 619 3489
Fragments arranged by size:
3489 1953 619
BsrBI CCG'CTC
Cuts at: 1394 1823 3193 3948 1394
Size: 429 1370 755 3507
Fragments arranged by size:
3507 1370 755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2629 4556 4730 65
Size: 1183 1381 1927 174 1396
Fragments arranged by size:
1927 1396 1381 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3047 3285 4705 4892 1700
Size: 110 1237 238 1420 187 2869
Fragments arranged by size:
2869 1420 1237 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6061
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6061
BssSI C'ACGA_G
Cuts at: 2598 4121 5133 2598
Size: 1523 1012 3526
Fragments arranged by size:
3526 1523 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3321 794
Size: 2527 3534
BstBI TT'CG_AA
Cuts at: 4865 4865
Size: 6061
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6061
BstXI CCAn_nnnn'nTGG
Cuts at: 3002 3002
Size: 6061
BstYI r'GATC_y
Cuts at: 1972 3020 3391 3758 4143 4160 5612 5698 5710
Size: 1048 371 367 385 17 1452 86 12
Cuts at: 5710 6056 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1048 385 371 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2965 513
Size: 552 939 961 3609
Fragments arranged by size:
3609 961 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5322
Fragments arranged by size:
5322 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4371 4391 3577
Size: 794 20 5247
Fragments arranged by size:
5247 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6026
DraI TTT'AAA
Cuts at: 3365 3365
Size: 6061
DraIII CAC_nnn'GTG
Cuts at: 3321 3321
Size: 6061
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5068 809
Size: 507 3752 1802
Fragments arranged by size:
3752 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2058 3213 3508
Size: 54 1425 50 275 18 228 1155 295
Cuts at: 3508 4397 6026 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1155 889 295 275 228 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3508 1537
Size: 275 1696 4090
Fragments arranged by size:
4090 1696 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2705 3991 1353
Size: 1352 1286 3423
Fragments arranged by size:
3423 1352 1286
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 4648 5022 5168 387
Size: 1064 138 867 2192 374 146 1280
Fragments arranged by size:
2192 1280 1064 867 374 146 138
EcoICRI GAG'CTC
Cuts at: 2221 2221
Size: 6061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2363 2366 2510 2658 2685 2861
Size: 393 575 156 3 144 148 27 176
Cuts at: 2861 4124 4721 5508 5776 1239
Size: 1263 597 787 268 1524
Fragments arranged by size:
1524 1263 787 597 575 393 268 176
156 148 144 27 3
EcoO109I rG'GnC_Cy
Cuts at: 2288 2288
Size: 6061
EcoRI G'AATT_C
Cuts at: 3278 3370 3748 3278
Size: 92 378 5591
Fragments arranged by size:
5591 378 92
FspI TGC'GCA
Cuts at: 4567 4567
Size: 6061
HaeII r_GCGC'y
Cuts at: 1678 2015 2850 5208 1678
Size: 337 835 2358 2531
Fragments arranged by size:
2531 2358 835 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4702 4734 4776 4808 1373
Size: 32 135 32 3130 32 42 32 2626
Fragments arranged by size:
3130 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 2992 3405 3651 669
Size: 988 281 537 517 413 246 3079
Fragments arranged by size:
3079 988 537 517 413 281 246
HindIII A'AGCT_T
Cuts at: 748 2295 2705 748
Size: 1547 410 4104
Fragments arranged by size:
4104 1547 410
HpaI GTT'AAC
Cuts at: 3651 3651
Size: 6061
KasI G'GCGC_C
Cuts at: 2011 2846 2011
Size: 835 5226
KpnI G_GTAC'C
Cuts at: 3386 3386
Size: 6061
MfeI C'AATT_G
Cuts at: 3660 3660
Size: 6061
MluI A'CGCG_T
Cuts at: 5937 5937
Size: 6061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5175 5359 1305
Size: 3870 184 2007
Fragments arranged by size:
3870 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4239
Fragments arranged by size:
4239 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4019 4378 4537 518
Size: 648 2853 359 159 2042
Fragments arranged by size:
2853 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2063 2155 2572 3477 4154 5302 5547 1606
Size: 457 92 417 905 677 1148 245 2120
Fragments arranged by size:
2120 1148 905 677 457 417 245 92
NarI GG'CG_CC
Cuts at: 2012 2847 2012
Size: 835 5226
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4570
Fragments arranged by size:
4570 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6061
NheI G'CTAG_C
Cuts at: 1052 2143 1052
Size: 1091 4970
NotI GC'GGCC_GC
Cuts at: 3508 3508
Size: 6061
NsiI A_TGCA'T
Cuts at: 2103 2103
Size: 6061
NspI r_CATG'y
Cuts at: 1132 1229 2101 2387 3223 3419 4964 1132
Size: 97 872 286 836 196 1545 2229
Fragments arranged by size:
2229 1545 872 836 286 196 97
PasI CC'CwG_GG
Cuts at: 1348 3026 1348
Size: 1678 4383
PciI A'CATG_T
Cuts at: 4960 4960
Size: 6061
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2257 1108
Size: 1149 4912
PmeI GTTT'AAAC
Cuts at: 3365 3365
Size: 6061
PmlI CAC'GTG
Cuts at: 3211 3211
Size: 6061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4130 4162 5643 5675 4130
Size: 32 1481 32 4516
Fragments arranged by size:
4516 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2288 2288
Size: 6061
PshAI GACnn'nnGTC
Cuts at: 1613 2418 1613
Size: 805 5256
PsiI TTA'TAA
Cuts at: 3631 3631
Size: 6061
PstI C_TGCA'G
Cuts at: 830 2772 3314 3413 830
Size: 1942 542 99 3478
Fragments arranged by size:
3478 1942 542 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6061
PvuII CAG'CTG
Cuts at: 2155 2572 2155
Size: 417 5644
SacI G_AGCT'C
Cuts at: 2223 2223
Size: 6061
SalI G'TCGA_C
Cuts at: 1936 2473 3403 1936
Size: 537 930 4594
Fragments arranged by size:
4594 930 537
SanDI GG'GwC_CC
Cuts at: 2288 2288
Size: 6061
SbfI CC_TGCA'GG
Cuts at: 3413 3413
Size: 6061
ScaI AGT'ACT
Cuts at: 2686 4309 6021 2686
Size: 1623 1712 2726
Fragments arranged by size:
2726 1712 1623
SfcI C'TryA_G
Cuts at: 826 1045 2768 2927 3310 3409 4544 5225 5416
Size: 219 1723 159 383 99 1135 681 191
Cuts at: 5416 826
Size: 1471
Fragments arranged by size:
1723 1471 1135 681 383 219 191 159
99
SfoI GGC'GCC
Cuts at: 2013 2848 2013
Size: 835 5226
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6061
SgrAI Cr'CCGG_yG
Cuts at: 1700 3285 1700
Size: 1585 4476
SmaI CCC'GGG
Cuts at: 1872 3388 1872
Size: 1516 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2416 3177 4165 5066 5328 820
Size: 197 926 473 761 988 901 262 1553
Fragments arranged by size:
1553 988 926 901 761 473 262 197
SnaBI TAC'GTA
Cuts at: 493 3852 493
Size: 3359 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6061
SphI G_CATG'C
Cuts at: 1132 2101 3419 1132
Size: 969 1318 3774
Fragments arranged by size:
3774 1318 969
SspI AAT'ATT
Cuts at: 5 52 3985 5
Size: 47 3933 2081
Fragments arranged by size:
3933 2081 47
StuI AGG'CCT
Cuts at: 1517 2960 1517
Size: 1443 4618
StyI C'CwwG_G
Cuts at: 513 1065 2004 2205 3500 513
Size: 552 939 201 1295 3074
Fragments arranged by size:
3074 1295 939 552 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3018 4088 4105 4258 4443 4888 325
Size: 1255 1438 1070 17 153 185 445 1498
Fragments arranged by size:
1498 1438 1255 1070 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2386 2684 4307
Size: 274 80 33 51 1443 409 298 1623
Cuts at: 4307 6019 96
Size: 1712 138
Fragments arranged by size:
1712 1623 1443 409 298 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2658 2690 3005 3037 6021 6053 2658
Size: 32 315 32 2984 32 2666
Fragments arranged by size:
2984 2666 315 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6061
XbaI T'CTAG_A
Cuts at: 3397 3397
Size: 6061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2857 2963 2857
Size: 106 5955
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6061
XmaI C'CCGG_G
Cuts at: 1870 3386 1870
Size: 1516 4545
XmnI GAAnn'nnTTC
Cuts at: 2181 4190 2181
Size: 2009 4052
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2722 3869 276
Size: 53 83 186 936 1188 1147 2468
Fragments arranged by size:
2468 1188 1147 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI
SrfI SwaI