Restriction map for
pFN21AE1908
(Circular) (Six-base) MAPSORT of: pFN21AE1908.seq Check: 2277 from: 1 to: 5950
pFN21AE1908 5950 bp
With 182 enzymes: SgfI *
January 31, 2011 17:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2171 2171
Size: 5950
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3760 278
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3293 1937
Size: 1356 4594
Acc65I G'GTAC_C
Cuts at: 3271 3271
Size: 5950
AclI AA'CG_TT
Cuts at: 1563 4077 4450 1563
Size: 2514 373 3063
Fragments arranged by size:
3063 2514 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4013 5397 5665 1632
Size: 2381 1384 268 1917
Fragments arranged by size:
2381 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5753
AflIII A'CryG_T
Cuts at: 1203 4849 5826 1203
Size: 3646 977 1327
Fragments arranged by size:
3646 1327 977
AgeI A'CCGG_T
Cuts at: 3141 4781 3141
Size: 1640 4310
AhdI GACnn_n'nnGTC
Cuts at: 4679 4679
Size: 5950
AleI CACnn'nnGTG
Cuts at: 1166 2954 1166
Size: 1788 4162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5950
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2465 2497 2465
Size: 32 5918
AlwNI CAG_nnn'CTG
Cuts at: 3693 5265 3693
Size: 1572 4378
ApaI G_GGCC'C
Cuts at: 2828 2828
Size: 5950
ApaLI G'TGCA_C
Cuts at: 2345 4007 5163 2345
Size: 1662 1156 3132
Fragments arranged by size:
3132 1662 1156
ApoI r'AATT_y
Cuts at: 1493 3259 3485 3637 5746 1493
Size: 1766 226 152 2109 1697
Fragments arranged by size:
2109 1766 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4504 160
Size: 872 3472 1606
Fragments arranged by size:
3472 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
AvaI C'yCGr_G
Cuts at: 1870 1943 2537 3005 3024 3171 3275 1870
Size: 73 594 468 19 147 104 4545
Fragments arranged by size:
4545 594 468 147 104 73 19
BamHI G'GATC_C
Cuts at: 3280 3647 3280
Size: 367 5583
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2013 2159 2448 3138 3271
Size: 325 217 627 226 146 289 690 133
Cuts at: 3271 4726 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 690 627 325 289 226 217
146 133
BanII G_rGCy'C
Cuts at: 1391 2537 2828 3269 1391
Size: 1146 291 441 4072
Fragments arranged by size:
4072 1146 441 291
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3025 928
Size: 971 1126 3853
Fragments arranged by size:
3853 1126 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4141 4175 1903
Size: 34 2204 34 3678
Fragments arranged by size:
3678 2204 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3842 5052 3842
Size: 1210 4740
BclI T'GATC_A
Cuts at: 1548 2847 1548
Size: 1299 4651
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3176 4561 136
Size: 107 122 71 631 605 1504 1385 1525
Fragments arranged by size:
1525 1504 1385 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5945 5945
Size: 5950
BlpI GC'TnA_GC
Cuts at: 3367 3367
Size: 5950
Bme1580I G_kGCm'C
Cuts at: 1790 2016 2349 2721 2828 3172 4011 5167 1790
Size: 226 333 372 107 344 839 1156 2573
Fragments arranged by size:
2573 1156 839 372 344 333 226 107
BmgBI CAC'GTC
Cuts at: 1318 2913 1318
Size: 1595 4355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3681 4639 442
Size: 416 53 83 399 24 2264 958 1753
Fragments arranged by size:
2264 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 4610 1239
Size: 824 2547 2579
Fragments arranged by size:
2579 2547 824
Bpu10I CC'TnA_GC
Cuts at: 2435 2435
Size: 5950
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4075 4940 5238 4075
Size: 865 298 4787
Fragments arranged by size:
4787 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 3025 4613 882
Size: 617 1413 113 1588 2219
Fragments arranged by size:
2219 1588 1413 617 113
BsaAI yAC'GTr
Cuts at: 493 1204 3741 493
Size: 711 2537 2702
Fragments arranged by size:
2702 2537 711
BsaBI GATnn'nnATC
Cuts at: 2696 5944 2696
Size: 3248 2702
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3757 4139 4774 275
Size: 53 83 186 936 2224 382 635 1451
Fragments arranged by size:
2224 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2445 3126 3141 4383 4781 5055 5202 5613 2445
Size: 681 15 1242 398 274 147 411 2782
Fragments arranged by size:
2782 1242 681 411 398 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 2465 2495 3032
Size: 30 165 30 972 30 60 30 537
Cuts at: 3032 3062 1178
Size: 30 4066
Fragments arranged by size:
4066 972 537 165 60 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2342 1186
Size: 1156 4794
BseYI C'CCAG_C
Cuts at: 2280 2709 5153 5649 2280
Size: 429 2444 496 2581
Fragments arranged by size:
2581 2444 496 429
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2587 2627 1163
Size: 573 851 40 4486
Fragments arranged by size:
4486 851 573 40
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3400 4161 5189 1540
Size: 275 186 1399 761 1028 2301
Fragments arranged by size:
2301 1399 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2349 2661 4011 4096 5167 2349
Size: 312 1350 85 1071 3132
Fragments arranged by size:
3132 1350 1071 312 85
BsmI GAATG_Cn'
Cuts at: 3461 3554 3461
Size: 93 5857
BsmBI CGTCTCn'nnnn_
Cuts at: 2033 2071 2033
Size: 38 5912
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2349 2537 2661 2721 2828 3172
Size: 399 226 333 188 124 60 107 344
Cuts at: 3172 3269 4011 4096 5167 1391
Size: 97 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 344 333 226 188
124 107 97 85 60
BspEI T'CCGG_A
Cuts at: 3126 5613 3126
Size: 2487 3463
BspHI T'CATG_A
Cuts at: 2886 3839 5569 2886
Size: 953 1730 3267
Fragments arranged by size:
3267 1730 953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2171 2559 2649 3305 844
Size: 1327 388 90 656 3489
Fragments arranged by size:
3489 1327 656 388 90
BsrBI CCG'CTC
Cuts at: 1394 1823 3837 1394
Size: 429 2014 3507
Fragments arranged by size:
3507 2014 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4445 4619 65
Size: 1183 3197 174 1396
Fragments arranged by size:
3197 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2676 3141 4594 4781 1700
Size: 110 866 465 1453 187 2869
Fragments arranged by size:
2869 1453 866 465 187 110
BsrGI T'GTAC_A
Cuts at: 96 2802 96
Size: 2706 3244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5950
BssSI C'ACGA_G
Cuts at: 4010 5022 4010
Size: 1012 4938
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2448 794
Size: 1654 4296
BstBI TT'CG_AA
Cuts at: 4754 4754
Size: 5950
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5950
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2836 2734
Size: 102 5848
BstYI r'GATC_y
Cuts at: 1972 2316 3280 3647 4032 4049 5501 5587 5599
Size: 344 964 367 385 17 1452 86 12
Cuts at: 5599 5945 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 964 385 367 346 344 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4318
Fragments arranged by size:
4318 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5211
Fragments arranged by size:
5211 683 56
BtsI GCAGTG_nn'
Cuts at: 2089 2138 3466 4260 4280 2089
Size: 49 1328 794 20 3759
Fragments arranged by size:
3759 1328 794 49 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5950
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5915
DraI TTT'AAA
Cuts at: 3254 3254
Size: 5950
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4957 809
Size: 507 3641 1802
Fragments arranged by size:
3641 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2268 3397
Size: 54 1425 50 275 18 279 159 1129
Cuts at: 3397 4286 5915 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1129 889 279 275 159 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3397 1537
Size: 275 1585 4090
Fragments arranged by size:
4090 1585 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3125 3880 1353
Size: 1772 755 3423
Fragments arranged by size:
3423 1772 755
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2473 4537 4911 5057 387
Size: 1064 138 884 2064 374 146 1280
Fragments arranged by size:
2064 1280 1064 884 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 4013 4610 5397 5665 1239
Size: 393 431 1950 597 787 268 1524
Fragments arranged by size:
1950 1524 787 597 431 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5950
EcoO109I rG'GnC_Cy
Cuts at: 2038 2824 2825 2038
Size: 786 1 5163
Fragments arranged by size:
5163 786 1
EcoRI G'AATT_C
Cuts at: 3259 3637 3259
Size: 378 5572
FspI TGC'GCA
Cuts at: 2498 2684 4456 2498
Size: 186 1772 3992
Fragments arranged by size:
3992 1772 186
HaeII r_GCGC'y
Cuts at: 1678 5097 1678
Size: 3419 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 3005 3037 3143
Size: 32 135 32 515 32 886 32 106
Cuts at: 3143 3175 4591 4623 4665 4697 1373
Size: 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 886 515 135 106 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3294 3540 669
Size: 988 281 1356 246 3079
Fragments arranged by size:
3079 1356 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5950
HpaI GTT'AAC
Cuts at: 3540 3540
Size: 5950
KpnI G_GTAC'C
Cuts at: 3275 3275
Size: 5950
MfeI C'AATT_G
Cuts at: 3549 3549
Size: 5950
MluI A'CGCG_T
Cuts at: 5826 5826
Size: 5950
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5064 5248 1305
Size: 3759 184 2007
Fragments arranged by size:
3759 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2111 2270 10
Size: 54 1425 343 279 159 3690
Fragments arranged by size:
3690 1425 343 279 159 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2352 2732 2885 2954 2984 3908 4267
Size: 648 1186 380 153 69 30 924 359
Cuts at: 4267 4426 518
Size: 159 2042
Fragments arranged by size:
2042 1186 924 648 380 359 159 153
69 30
MspA1I CmG'CkG
Cuts at: 1606 2280 3366 4043 5191 5436 1606
Size: 674 1086 677 1148 245 2120
Fragments arranged by size:
2120 1148 1086 677 674 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4459
Fragments arranged by size:
4459 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5950
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5950
NotI GC'GGCC_GC
Cuts at: 3397 3397
Size: 5950
NspI r_CATG'y
Cuts at: 1132 1229 2989 3308 4853 1132
Size: 97 1760 319 1545 2229
Fragments arranged by size:
2229 1760 1545 319 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5950
PciI A'CATG_T
Cuts at: 4849 4849
Size: 5950
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5950
PfoI T'CCnGG_A
Cuts at: 2079 3054 2079
Size: 975 4975
PmeI GTTT'AAAC
Cuts at: 3254 3254
Size: 5950
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2051 2083 2465 2497 4019 4051 5532 5564 2051
Size: 32 382 32 1522 32 1481 32 2437
Fragments arranged by size:
2437 1522 1481 382 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5950
PsiI TTA'TAA
Cuts at: 3520 3520
Size: 5950
PspOMI G'GGCC_C
Cuts at: 2824 2824
Size: 5950
PstI C_TGCA'G
Cuts at: 830 2133 3302 830
Size: 1303 1169 3478
Fragments arranged by size:
3478 1303 1169
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5950
PvuII CAG'CTG
Cuts at: 2280 2280
Size: 5950
SalI G'TCGA_C
Cuts at: 1936 3292 1936
Size: 1356 4594
SbfI CC_TGCA'GG
Cuts at: 3302 3302
Size: 5950
ScaI AGT'ACT
Cuts at: 4198 5910 4198
Size: 1712 4238
SexAI A'CCwGG_T
Cuts at: 2507 2507
Size: 5950
SfcI C'TryA_G
Cuts at: 826 1045 2129 3298 4433 5114 5305 826
Size: 219 1084 1169 1135 681 191 1471
Fragments arranged by size:
1471 1169 1135 1084 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5950
SgrAI Cr'CCGG_yG
Cuts at: 1700 3141 1700
Size: 1441 4509
SmaI CCC'GGG
Cuts at: 1872 3007 3026 3277 1872
Size: 1135 19 251 4545
Fragments arranged by size:
4545 1135 251 19
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4054 4955 5217 820
Size: 197 926 2111 901 262 1553
Fragments arranged by size:
2111 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3741 493
Size: 3248 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5950
SphI G_CATG'C
Cuts at: 1132 3308 1132
Size: 2176 3774
SspI AAT'ATT
Cuts at: 5 52 3874 5
Size: 47 3822 2081
Fragments arranged by size:
3822 2081 47
StuI AGG'CCT
Cuts at: 1517 2794 1517
Size: 1277 4673
StyI C'CwwG_G
Cuts at: 513 1065 2004 3389 513
Size: 552 939 1385 3074
Fragments arranged by size:
3074 1385 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3977 3994 4147 4332 4777 325
Size: 1255 2397 17 153 185 445 1498
Fragments arranged by size:
2397 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2757 2802 2928
Size: 274 80 33 51 1443 780 45 126
Cuts at: 2928 4196 5908 96
Size: 1268 1712 138
Fragments arranged by size:
1712 1443 1268 780 274 138 126 80
51 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 5910 5942 2375
Size: 32 3503 32 2383
Fragments arranged by size:
3503 2383 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5950
XbaI T'CTAG_A
Cuts at: 3286 3286
Size: 5950
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2512 2512
Size: 5950
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5950
XmaI C'CCGG_G
Cuts at: 1870 3005 3024 3275 1870
Size: 1135 19 251 4545
Fragments arranged by size:
4545 1135 251 19
XmnI GAAnn'nnTTC
Cuts at: 4079 4079
Size: 5950
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3758 276
Size: 53 83 186 936 2224 2468
Fragments arranged by size:
2468 2224 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI