Restriction map for
pFN21AE1904
(Circular) (Six-base) MAPSORT of: pFN21AE1904.seq Check: 5813 from: 1 to: 6007
pFN21AE1904 6007 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3817 278
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3350 1937
Size: 1413 4594
Acc65I G'GTAC_C
Cuts at: 3328 3328
Size: 6007
AclI AA'CG_TT
Cuts at: 1563 4134 4507 1563
Size: 2571 373 3063
Fragments arranged by size:
3063 2571 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4070 5454 5722 1632
Size: 2438 1384 268 1917
Fragments arranged by size:
2438 1917 1384 268
AfeI AGC'GCT
Cuts at: 2402 2402
Size: 6007
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5810
AflIII A'CryG_T
Cuts at: 1203 3264 4906 5883 1203
Size: 2061 1642 977 1327
Fragments arranged by size:
2061 1642 1327 977
AgeI A'CCGG_T
Cuts at: 4838 4838
Size: 6007
AhdI GACnn_n'nnGTC
Cuts at: 4736 4736
Size: 6007
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6007
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2388 2420 2388
Size: 32 5975
AlwNI CAG_nnn'CTG
Cuts at: 3750 5322 3750
Size: 1572 4435
ApaI G_GGCC'C
Cuts at: 2217 2217
Size: 6007
ApaLI G'TGCA_C
Cuts at: 4064 5220 4064
Size: 1156 4851
ApoI r'AATT_y
Cuts at: 1493 2490 2557 2771 3144 3230 3316 3542 3694
Size: 997 67 214 373 86 86 226 152
Cuts at: 3694 5803 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 997 373 226 214 152 86
86 67
AseI AT'TA_AT
Cuts at: 160 1032 4561 160
Size: 872 3529 1606
Fragments arranged by size:
3529 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
AvaI C'yCGr_G
Cuts at: 1870 1943 2178 3332 1870
Size: 73 235 1154 4545
Fragments arranged by size:
4545 1154 235 73
BamHI G'GATC_C
Cuts at: 3337 3704 3337
Size: 367 5640
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3328 4783 618
Size: 325 217 627 1541 1455 1842
Fragments arranged by size:
1842 1541 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2217 2787 3326 1391
Size: 826 570 539 4072
Fragments arranged by size:
4072 826 570 539
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5036
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4198 4232 1903
Size: 34 50 34 2177 34 3678
Fragments arranged by size:
3678 2177 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3275 3899 5109 3275
Size: 624 1210 4173
Fragments arranged by size:
4173 1210 624
BclI T'GATC_A
Cuts at: 1548 2323 1548
Size: 775 5232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4618 136
Size: 107 122 71 631 605 2946 1525
Fragments arranged by size:
2946 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2719 6002 2719
Size: 3283 2724
BlpI GC'TnA_GC
Cuts at: 3424 3424
Size: 6007
Bme1580I G_kGCm'C
Cuts at: 1790 2217 4068 5224 1790
Size: 427 1851 1156 2573
Fragments arranged by size:
2573 1851 1156 427
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6007
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2140 3738 4696
Size: 416 53 83 399 24 723 1598 958
Cuts at: 4696 442
Size: 1753
Fragments arranged by size:
1753 1598 958 723 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6007
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2211 2211
Size: 6007
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2277 4667 1239
Size: 1038 2390 2579
Fragments arranged by size:
2579 2390 1038
Bpu10I CC'TnA_GC
Cuts at: 2196 3050 2196
Size: 854 5153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2762 4132 4997 5295 2762
Size: 1370 865 298 3474
Fragments arranged by size:
3474 1370 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4670 882
Size: 617 3171 2219
Fragments arranged by size:
3171 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3798 493
Size: 711 2594 2702
Fragments arranged by size:
2702 2594 711
BsaBI GATnn'nnATC
Cuts at: 3286 6001 3286
Size: 2715 3292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3814 4196 4831 275
Size: 53 83 186 936 2281 382 635 1451
Fragments arranged by size:
2281 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4440 4838 5112 5259 5670 4440
Size: 398 274 147 411 4777
Fragments arranged by size:
4777 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3218 3248 1178
Size: 30 165 30 1815 30 3937
Fragments arranged by size:
3937 1815 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 2266 2376 1186
Size: 927 153 110 4817
Fragments arranged by size:
4817 927 153 110
BseYI C'CCAG_C
Cuts at: 5210 5706 5210
Size: 496 5511
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2069 1163
Size: 573 333 5101
Fragments arranged by size:
5101 573 333
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3457 4218 5246 1540
Size: 275 186 1456 761 1028 2301
Fragments arranged by size:
2301 1456 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4068 4153 5224 4068
Size: 85 1071 4851
Fragments arranged by size:
4851 1071 85
BsmI GAATG_Cn'
Cuts at: 2035 3518 3611 2035
Size: 1483 93 4431
Fragments arranged by size:
4431 1483 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2217 2787 3326 4068 4153 5224 1391
Size: 399 427 570 539 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 570 539 427 399 85
BspEI T'CCGG_A
Cuts at: 5670 5670
Size: 6007
BspHI T'CATG_A
Cuts at: 3896 5626 3896
Size: 1730 4277
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3362 844
Size: 2518 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2195 3894 1394
Size: 429 372 1699 3507
Fragments arranged by size:
3507 1699 429 372
BsrDI GCAATG_nn'
Cuts at: 65 1248 4502 4676 65
Size: 1183 3254 174 1396
Fragments arranged by size:
3254 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4651 4838 1700
Size: 110 2841 187 2869
Fragments arranged by size:
2869 2841 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6007
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6007
BssSI C'ACGA_G
Cuts at: 2496 4067 5079 2496
Size: 1571 1012 3424
Fragments arranged by size:
3424 1571 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6007
BstBI TT'CG_AA
Cuts at: 2803 4811 2803
Size: 2008 3999
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6007
BstXI CCAn_nnnn'nTGG
Cuts at: 2631 3278 2631
Size: 647 5360
BstYI r'GATC_y
Cuts at: 1972 2058 2719 2757 3337 3704 4089 4106 5558
Size: 86 661 38 580 367 385 17 1452
Cuts at: 5558 5644 5656 6002 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 661 580 385 367 346 86
86 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2371 513
Size: 552 1306 4149
Fragments arranged by size:
4149 1306 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5268
Fragments arranged by size:
5268 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 4317 4337 3523
Size: 794 20 5193
Fragments arranged by size:
5193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6007
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2926 2961 529
Size: 35 2362 35 3575
Fragments arranged by size:
3575 2362 35 35
DraI TTT'AAA
Cuts at: 2316 3311 2316
Size: 995 5012
DraIII CAC_nnn'GTG
Cuts at: 2850 2850
Size: 6007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5014 809
Size: 507 3698 1802
Fragments arranged by size:
3698 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2633 2820 2934
Size: 54 1425 50 275 18 803 187 114
Cuts at: 2934 3151 3454 4343 5972 8
Size: 217 303 889 1629 43
Fragments arranged by size:
1629 1425 889 803 303 275 217 187
114 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3454 1537
Size: 275 1642 4090
Fragments arranged by size:
4090 1642 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2885 3091 3169 3301 3937 1353
Size: 1532 206 78 132 636 3423
Fragments arranged by size:
3423 1532 636 206 132 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4594 4968 5114 387
Size: 1064 138 3005 374 146 1280
Fragments arranged by size:
3005 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 4070 4667 5454 5722 1239
Size: 393 645 1793 597 787 268 1524
Fragments arranged by size:
1793 1524 787 645 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2206 2214 2743 2206
Size: 8 529 5470
Fragments arranged by size:
5470 529 8
EcoRI G'AATT_C
Cuts at: 3316 3694 3316
Size: 378 5629
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2351 2351
Size: 6007
FspI TGC'GCA
Cuts at: 4513 4513
Size: 6007
HaeII r_GCGC'y
Cuts at: 1678 2138 2404 5154 1678
Size: 460 266 2750 2531
Fragments arranged by size:
2750 2531 460 266
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3116 3148 4648 4680 4722
Size: 32 135 32 1544 32 1500 32 42
Cuts at: 4722 4754 1373
Size: 32 2626
Fragments arranged by size:
2626 1544 1500 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2674 3351 3597 669
Size: 988 281 736 677 246 3079
Fragments arranged by size:
3079 988 736 677 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6007
HpaI GTT'AAC
Cuts at: 3597 3597
Size: 6007
KpnI G_GTAC'C
Cuts at: 3332 3332
Size: 6007
MfeI C'AATT_G
Cuts at: 3606 3606
Size: 6007
MluI A'CGCG_T
Cuts at: 5883 5883
Size: 6007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3261 5121 5305 1305
Size: 1956 1860 184 2007
Fragments arranged by size:
2007 1956 1860 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2635 2822 2936 3153 10
Size: 54 1425 343 803 187 114 217 2864
Fragments arranged by size:
2864 1425 803 343 217 187 114 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3965 4324 4483 518
Size: 648 2799 359 159 2042
Fragments arranged by size:
2799 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3423 4100 5248 5493 1606
Size: 1817 677 1148 245 2120
Fragments arranged by size:
2120 1817 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2371 513
Size: 552 1306 4149
Fragments arranged by size:
4149 1306 552
NdeI CA'TA_TG
Cuts at: 387 2691 387
Size: 2304 3703
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6007
NotI GC'GGCC_GC
Cuts at: 3454 3454
Size: 6007
NspI r_CATG'y
Cuts at: 1132 1229 2266 3268 3365 4910 1132
Size: 97 1037 1002 97 1545 2229
Fragments arranged by size:
2229 1545 1037 1002 97 97
PasI CC'CwG_GG
Cuts at: 1348 2210 2624 1348
Size: 862 414 4731
Fragments arranged by size:
4731 862 414
PciI A'CATG_T
Cuts at: 3264 4906 3264
Size: 1642 4365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6007
PmeI GTTT'AAAC
Cuts at: 3311 3311
Size: 6007
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4076 4108 5589 5621 4076
Size: 32 1481 32 4462
Fragments arranged by size:
4462 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2743 2206
Size: 537 5470
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6007
PsiI TTA'TAA
Cuts at: 2477 3577 2477
Size: 1100 4907
PspOMI G'GGCC_C
Cuts at: 2213 2213
Size: 6007
PstI C_TGCA'G
Cuts at: 830 3359 830
Size: 2529 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6007
SalI G'TCGA_C
Cuts at: 1936 3349 1936
Size: 1413 4594
SapI GCTCTTCn'nnn_
Cuts at: 3091 3091
Size: 6007
SbfI CC_TGCA'GG
Cuts at: 3359 3359
Size: 6007
ScaI AGT'ACT
Cuts at: 4255 5967 4255
Size: 1712 4295
SfcI C'TryA_G
Cuts at: 826 1045 2736 3355 4490 5171 5362 826
Size: 219 1691 619 1135 681 191 1471
Fragments arranged by size:
1691 1471 1135 681 619 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6007
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6007
SmaI CCC'GGG
Cuts at: 1872 3334 1872
Size: 1462 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2178 2777 4111 5012 5274 820
Size: 197 926 235 599 1334 901 262 1553
Fragments arranged by size:
1553 1334 926 901 599 262 235 197
SnaBI TAC'GTA
Cuts at: 493 3798 493
Size: 3305 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6007
SphI G_CATG'C
Cuts at: 1132 2266 3365 1132
Size: 1134 1099 3774
Fragments arranged by size:
3774 1134 1099
SspI AAT'ATT
Cuts at: 5 52 3931 5
Size: 47 3879 2081
Fragments arranged by size:
3879 2081 47
StuI AGG'CCT
Cuts at: 1517 2082 1517
Size: 565 5442
StyI C'CwwG_G
Cuts at: 513 1065 2335 2371 3446 513
Size: 552 1270 36 1075 3074
Fragments arranged by size:
3074 1270 1075 552 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3025 3130 4034 4051 4204 4389 4834
Size: 1255 1445 105 904 17 153 185 445
Cuts at: 4834 325
Size: 1498
Fragments arranged by size:
1498 1445 1255 904 445 185 153 105
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4253 5965 96
Size: 274 80 33 51 1443 2276 1712 138
Fragments arranged by size:
2276 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3218 3250 5967 5999 3218
Size: 32 2717 32 3226
Fragments arranged by size:
3226 2717 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6007
XbaI T'CTAG_A
Cuts at: 3343 3343
Size: 6007
XhoI C'TCGA_G
Cuts at: 1943 2178 1943
Size: 235 5772
XmaI C'CCGG_G
Cuts at: 1870 3332 1870
Size: 1462 4545
XmnI GAAnn'nnTTC
Cuts at: 4136 4136
Size: 6007
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3815 276
Size: 53 83 186 936 2281 2468
Fragments arranged by size:
2468 2281 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI