Restriction map for
pFN21AE1901
(Circular) (Six-base) MAPSORT of: pFN21AE1901.seq Check: 9096 from: 1 to: 5992
pFN21AE1901 5992 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3802 278
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3215 3313 3215
Size: 98 5894
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4055 5439 5707 1632
Size: 2423 1384 268 1917
Fragments arranged by size:
2423 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 4891 5868 1203
Size: 3688 977 1327
Fragments arranged by size:
3688 1327 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 4721 4721
Size: 5992
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 3735 5307 3735
Size: 1572 4420
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 2867 3301 3527 3679 5788 1493
Size: 1374 434 226 152 2109 1697
Fragments arranged by size:
2109 1697 1374 434 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2162 4546 160
Size: 872 1130 2384 1606
Fragments arranged by size:
2384 1606 1130 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 3317 1870
Size: 73 1374 4545
Fragments arranged by size:
4545 1374 73
BamHI G'GATC_C
Cuts at: 2060 3322 3689 2060
Size: 1262 367 4363
Fragments arranged by size:
4363 1262 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2377 3215 3313 4768 618
Size: 325 217 627 590 838 98 1455 1842
Fragments arranged by size:
1842 1455 838 627 590 325 217 98
BanII G_rGCy'C
Cuts at: 1391 2044 3311 1391
Size: 653 1267 4072
Fragments arranged by size:
4072 1267 653
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2292 928
Size: 971 393 4628
Fragments arranged by size:
4628 971 393
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BfrBI ATG'CAT
Cuts at: 2332 2332
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4603 136
Size: 107 122 71 631 605 2931 1525
Fragments arranged by size:
2931 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5987 5987
Size: 5992
BlpI GC'TnA_GC
Cuts at: 3409 3409
Size: 5992
Bme1580I G_kGCm'C
Cuts at: 1790 4053 5209 1790
Size: 2263 1156 2573
Fragments arranged by size:
2573 2263 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 2511 3723
Size: 416 53 83 399 24 882 212 1212
Cuts at: 3723 4681 442
Size: 958 1753
Fragments arranged by size:
1753 1212 958 882 416 399 212 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2619 2688 4652 1239
Size: 1380 69 1964 2579
Fragments arranged by size:
2579 1964 1380 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3038 4655 882
Size: 617 1539 1617 2219
Fragments arranged by size:
2219 1617 1539 617
BsaAI yAC'GTr
Cuts at: 493 1204 3783 493
Size: 711 2579 2702
Fragments arranged by size:
2702 2579 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3799 4181 4816 275
Size: 53 83 186 936 2266 382 635 1451
Fragments arranged by size:
2266 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2019 2031 2049 2061 1178
Size: 30 165 30 616 12 18 12 5109
Fragments arranged by size:
5109 616 165 30 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3111 1186
Size: 1925 4067
BseYI C'CCAG_C
Cuts at: 5195 5691 5195
Size: 496 5496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2641 2826 2919 1163
Size: 573 905 185 93 4236
Fragments arranged by size:
4236 905 573 185 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3442 4203 5231 1540
Size: 275 186 1441 761 1028 2301
Fragments arranged by size:
2301 1441 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4053 4138 5209 4053
Size: 85 1071 4836
Fragments arranged by size:
4836 1071 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 3311 4053 4138 5209 1391
Size: 399 254 1267 742 85 1071 2174
Fragments arranged by size:
2174 1267 1071 742 399 254 85
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3347 844
Size: 2503 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2010 2905 3879 1394
Size: 429 187 895 974 3507
Fragments arranged by size:
3507 974 895 429 187
BsrDI GCAATG_nn'
Cuts at: 65 1248 4487 4661 65
Size: 1183 3239 174 1396
Fragments arranged by size:
3239 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4636 4823 1700
Size: 110 2826 187 2869
Fragments arranged by size:
2869 2826 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5992
BssSI C'ACGA_G
Cuts at: 4052 5064 4052
Size: 1012 4980
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5992
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2712 2712
Size: 5992
BstYI r'GATC_y
Cuts at: 1972 2060 2699 3322 3689 4074 4091 5543 5629
Size: 88 639 623 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 639 623 385 367 346 88
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2736 2736
Size: 5992
BtgI C'CryG_G
Cuts at: 513 1065 2381 513
Size: 552 1316 4124
Fragments arranged by size:
4124 1316 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5253
Fragments arranged by size:
5253 683 56
BtsI GCAGTG_nn'
Cuts at: 2535 2624 3049 3275 3508 4302 4322 2535
Size: 89 425 226 233 794 20 4205
Fragments arranged by size:
4205 794 425 233 226 89 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2983 3018 3212 3247 529
Size: 35 2419 35 194 35 3274
Fragments arranged by size:
3274 2419 194 35 35 35
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3439 4328 5957
Size: 54 1425 50 275 18 1609 889 1629
Cuts at: 5957 8
Size: 43
Fragments arranged by size:
1629 1609 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3439 1537
Size: 275 1627 4090
Fragments arranged by size:
4090 1627 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2636 3922 1353
Size: 1283 1286 3423
Fragments arranged by size:
3423 1286 1283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2619 2688 4055 4652 5439 5707 1239
Size: 393 987 69 1367 597 787 268 1524
Fragments arranged by size:
1524 1367 987 787 597 393 268 69
EcoRI G'AATT_C
Cuts at: 2867 3301 3679 2867
Size: 434 378 5180
Fragments arranged by size:
5180 434 378
EcoRV GAT'ATC
Cuts at: 3022 3022
Size: 5992
FspI TGC'GCA
Cuts at: 4498 4498
Size: 5992
HaeII r_GCGC'y
Cuts at: 1678 5139 1678
Size: 3461 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 3018 3050 4633
Size: 32 135 32 473 32 941 32 1583
Cuts at: 4633 4665 4707 4739 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1583 941 473 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2793 748
Size: 2045 3947
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KpnI G_GTAC'C
Cuts at: 3219 3317 3219
Size: 98 5894
MfeI C'AATT_G
Cuts at: 2884 3591 2884
Size: 707 5285
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2017 5106 5290 1305
Size: 712 3089 184 2007
Fragments arranged by size:
3089 2007 712 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4170
Fragments arranged by size:
4170 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2162 3950 4309 4468 518
Size: 648 996 1788 359 159 2042
Fragments arranged by size:
2042 1788 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3408 4085 5233 5478 1606
Size: 1802 677 1148 245 2120
Fragments arranged by size:
2120 1802 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5440
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NsiI A_TGCA'T
Cuts at: 2334 2334
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2336 3350 4895 1132
Size: 97 1107 1014 1545 2229
Fragments arranged by size:
2229 1545 1107 1014 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 4891 4891
Size: 5992
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PfoI T'CCnGG_A
Cuts at: 2781 2781
Size: 5992
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2470 3344 830
Size: 1640 874 3478
Fragments arranged by size:
3478 1640 874
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SbfI CC_TGCA'GG
Cuts at: 3344 3344
Size: 5992
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 3028 3028
Size: 5992
SfcI C'TryA_G
Cuts at: 826 1045 2118 2466 3340 4475 5156 5347 826
Size: 219 1073 348 874 1135 681 191 1471
Fragments arranged by size:
1471 1135 1073 874 681 348 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 3319 1872
Size: 1447 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4096 4997 5259 820
Size: 197 926 2153 901 262 1553
Fragments arranged by size:
2153 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 3350 1132
Size: 2218 3774
SspI AAT'ATT
Cuts at: 5 52 2647 3916 5
Size: 47 2595 1269 2081
Fragments arranged by size:
2595 2081 1269 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5992
StyI C'CwwG_G
Cuts at: 513 1065 2103 3431 513
Size: 552 1038 1328 3074
Fragments arranged by size:
3074 1328 1038 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4019 4036 4189 4374 4819 325
Size: 1255 2439 17 153 185 445 1498
Fragments arranged by size:
2439 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4238 5950 96
Size: 274 80 33 51 1443 2261 1712 138
Fragments arranged by size:
2261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2527 2559 3256 3288 5952 5984 2527
Size: 32 697 32 2664 32 2535
Fragments arranged by size:
2664 2535 697 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3030 1316
Size: 1714 4278
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5992
XmaI C'CCGG_G
Cuts at: 1870 3317 1870
Size: 1447 4545
XmnI GAAnn'nnTTC
Cuts at: 2655 2688 4121 2655
Size: 33 1433 4526
Fragments arranged by size:
4526 1433 33
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3800 276
Size: 53 83 186 936 2266 2468
Fragments arranged by size:
2468 2266 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI XcmI