Restriction map for
pFN21AE1899
(Circular) (Six-base) MAPSORT of: pFN21AE1899.seq Check: 9332 from: 1 to: 7180
pFN21AE1899 7180 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2050 2111 2274 2050
Size: 61 163 6956
Fragments arranged by size:
6956 163 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4990 278
Size: 53 83 186 936 3454 2468
Fragments arranged by size:
3454 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4140 4523 1937
Size: 2203 383 4594
Fragments arranged by size:
4594 2203 383
Acc65I G'GTAC_C
Cuts at: 4501 4501
Size: 7180
AclI AA'CG_TT
Cuts at: 1563 5307 5680 1563
Size: 3744 373 3063
Fragments arranged by size:
3744 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2597 3487 5243 6627 6895 1632
Size: 965 890 1756 1384 268 1917
Fragments arranged by size:
1917 1756 1384 965 890 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6983
AflIII A'CryG_T
Cuts at: 1203 2220 2222 6079 7056 1203
Size: 1017 2 3857 977 1327
Fragments arranged by size:
3857 1327 1017 977 2
AgeI A'CCGG_T
Cuts at: 6011 6011
Size: 7180
AhdI GACnn_n'nnGTC
Cuts at: 3435 5909 3435
Size: 2474 4706
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2538 2570 4433 4465 2538
Size: 32 1863 32 5253
Fragments arranged by size:
5253 1863 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2329 2332 3950 4923 6495 2329
Size: 3 1618 973 1572 3014
Fragments arranged by size:
3014 1618 1572 973 3
ApaI G_GGCC'C
Cuts at: 2380 3363 4288 2380
Size: 983 925 5272
Fragments arranged by size:
5272 983 925
ApaLI G'TGCA_C
Cuts at: 5237 6393 5237
Size: 1156 6024
ApoI r'AATT_y
Cuts at: 1493 4489 4715 4867 6976 1493
Size: 2996 226 152 2109 1697
Fragments arranged by size:
2996 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5734 160
Size: 872 4702 1606
Fragments arranged by size:
4702 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7180
AvaI C'yCGr_G
Cuts at: 1870 1943 2877 3587 4351 4505 1870
Size: 73 934 710 764 154 4545
Fragments arranged by size:
4545 934 764 710 154 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2209 2242 2209
Size: 33 7147
BamHI G'GATC_C
Cuts at: 4510 4877 4510
Size: 367 6813
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3027 3370 3761 4345 4501
Size: 325 217 627 1240 343 391 584 156
Cuts at: 4501 5956 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1240 627 584 391 343 325
217 156
BanII G_rGCy'C
Cuts at: 1391 2028 2100 2178 2345 2380 2620 2654 2688
Size: 637 72 78 167 35 240 34 34
Cuts at: 2688 3011 3363 4007 4288 4418 4499 1391
Size: 323 352 644 281 130 81 4072
Fragments arranged by size:
4072 644 637 352 323 281 240 167
130 81 78 72 35 34 34
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6209
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2806 2840 5371 5405 1903
Size: 34 869 34 2531 34 3678
Fragments arranged by size:
3678 2531 869 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2237 2454 5072 6282 2237
Size: 217 2618 1210 3135
Fragments arranged by size:
3135 2618 1210 217
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7180
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4343 5791 136
Size: 107 122 71 631 605 2671 1448 1525
Fragments arranged by size:
2671 1525 1448 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7175 7175
Size: 7180
BlpI GC'TnA_GC
Cuts at: 4597 4597
Size: 7180
Bme1580I G_kGCm'C
Cuts at: 1790 2380 2602 2903 3032 3363 3633 4288 5241
Size: 590 222 301 129 331 270 655 953
Cuts at: 5241 6397 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 953 655 590 331 301 270
222 129
BmgBI CAC'GTC
Cuts at: 1318 2281 1318
Size: 963 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3026 3938 4911
Size: 416 53 83 399 24 1609 912 973
Cuts at: 4911 5869 442
Size: 958 1753
Fragments arranged by size:
1753 1609 973 958 912 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3879 1056
Size: 2823 4357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2452 2659 2966 3425 3593 3665 4064 4284
Size: 1213 207 307 459 168 72 399 220
Cuts at: 4284 4337 5840 1239
Size: 53 1503 2579
Fragments arranged by size:
2579 1503 1213 459 399 307 220 207
168 72 53
Bpu10I CC'TnA_GC
Cuts at: 2850 3515 3639 2850
Size: 665 124 6391
Fragments arranged by size:
6391 665 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3808 4402 5305 6170 6468 3808
Size: 594 903 865 298 4520
Fragments arranged by size:
4520 903 865 594 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4480 5843 882
Size: 617 2981 1363 2219
Fragments arranged by size:
2981 2219 1363 617
BsaAI yAC'GTr
Cuts at: 493 1204 2223 4971 493
Size: 711 1019 2748 2702
Fragments arranged by size:
2748 2702 1019 711
BsaBI GATnn'nnATC
Cuts at: 2970 7174 2970
Size: 4204 2976
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2712 3464 4987 5369
Size: 53 83 186 936 1179 752 1523 382
Cuts at: 5369 6004 275
Size: 635 1451
Fragments arranged by size:
1523 1451 1179 936 752 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5613 6011 6285 6432 6843 5613
Size: 398 274 147 411 5950
Fragments arranged by size:
5950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2538 2568 2858 2888 3142
Size: 30 165 30 1135 30 290 30 254
Cuts at: 3142 3172 4433 4463 1178
Size: 30 1261 30 3895
Fragments arranged by size:
3895 1261 1135 290 254 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3134 3137 3140 3143 3269 1186
Size: 1948 3 3 3 126 5097
Fragments arranged by size:
5097 1948 126 3 3 3
BseYI C'CCAG_C
Cuts at: 2318 2354 2654 2707 2809 3541 3606 6383 6879
Size: 36 300 53 102 732 65 2777 496
Cuts at: 6879 2318
Size: 2619
Fragments arranged by size:
2777 2619 732 496 300 102 65 53
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3375 1163
Size: 573 1639 4968
Fragments arranged by size:
4968 1639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4630 5391 6419 1540
Size: 275 186 2629 761 1028 2301
Fragments arranged by size:
2629 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2178 2952 3328 4007 4035 4418 5241 5326 6397
Size: 774 376 679 28 383 823 85 1071
Cuts at: 6397 2178
Size: 2961
Fragments arranged by size:
2961 1071 823 774 679 383 376 85
28
BsmI GAATG_Cn'
Cuts at: 4691 4784 4691
Size: 93 7087
BsmBI CGTCTCn'nnnn_
Cuts at: 2113 3455 3712 2113
Size: 1342 257 5581
Fragments arranged by size:
5581 1342 257
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2100 2178 2345 2380 2602 2620
Size: 399 238 72 78 167 35 222 18
Cuts at: 2620 2654 2688 2903 2952 3011 3032 3328 3363
Size: 34 34 215 49 59 21 296 35
Cuts at: 3363 3633 4007 4035 4288 4418 4499 5241 5326
Size: 270 374 28 253 130 81 742 85
Cuts at: 5326 6397 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 296 270 253
238 222 215 167 130 85 81 78
72 59 49 35 35 34 34 28
21 18
BspEI T'CCGG_A
Cuts at: 6843 6843
Size: 7180
BspHI T'CATG_A
Cuts at: 5069 6799 5069
Size: 1730 5450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2050 2111 2274 3252 4163 4535 844
Size: 1206 61 163 978 911 372 3489
Fragments arranged by size:
3489 1206 978 911 372 163 61
BsrBI CCG'CTC
Cuts at: 1394 1823 5067 1394
Size: 429 3244 3507
Fragments arranged by size:
3507 3244 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4411 5675 5849 65
Size: 1183 3163 1264 174 1396
Fragments arranged by size:
3163 1396 1264 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5824 6011 1700
Size: 110 4014 187 2869
Fragments arranged by size:
4014 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3739 96
Size: 3643 3537
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7180
BssSI C'ACGA_G
Cuts at: 2825 5240 6252 2825
Size: 2415 1012 3753
Fragments arranged by size:
3753 2415 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 2704 794
Size: 1535 375 5270
Fragments arranged by size:
5270 1535 375
BstBI TT'CG_AA
Cuts at: 5984 5984
Size: 7180
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7180
BstXI CCAn_nnnn'nTGG
Cuts at: 3011 3541 3571 3651 3778 4199 3011
Size: 530 30 80 127 421 5992
Fragments arranged by size:
5992 530 421 127 80 30
BstYI r'GATC_y
Cuts at: 1972 2971 3660 4510 4877 5262 5279 6731 6817
Size: 999 689 850 367 385 17 1452 86
Cuts at: 6817 6829 7175 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 999 850 689 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4280 4280
Size: 7180
BtgI C'CryG_G
Cuts at: 513 1065 2004 4452 513
Size: 552 939 2448 3241
Fragments arranged by size:
3241 2448 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 3097 3147 3460 487
Size: 683 56 1319 552 50 313 4207
Fragments arranged by size:
4207 1319 683 552 313 56 50
BtsI GCAGTG_nn'
Cuts at: 3554 3847 3886 4135 4696 5490 5510 3554
Size: 293 39 249 561 794 20 5224
Fragments arranged by size:
5224 794 561 293 249 39 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7180
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7145
DraI TTT'AAA
Cuts at: 4484 4484
Size: 7180
DraIII CAC_nnn'GTG
Cuts at: 2664 2664
Size: 7180
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6187 809
Size: 507 4871 1802
Fragments arranged by size:
4871 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2739 3059 3561
Size: 54 1425 50 275 18 909 320 502
Cuts at: 3561 4168 4189 4204 4221 4627 5516 7145 8
Size: 607 21 15 17 406 889 1629 43
Fragments arranged by size:
1629 1425 909 889 607 502 406 320
275 54 50 43 21 18 17 15
EagI C'GGCC_G
Cuts at: 1537 1812 4627 1537
Size: 275 2815 4090
Fragments arranged by size:
4090 2815 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3882 4361 5110 1353
Size: 2529 479 749 3423
Fragments arranged by size:
3423 2529 749 479
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 3656 5767 6141 6287 387
Size: 1064 138 579 1488 2111 374 146 1280
Fragments arranged by size:
2111 1488 1280 1064 579 374 146 138
EcoICRI GAG'CTC
Cuts at: 2176 4005 4416 2176
Size: 1829 411 4940
Fragments arranged by size:
4940 1829 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2452 2597 2659 2966 3425 3487 3593
Size: 393 820 145 62 307 459 62 106
Cuts at: 3593 3665 4064 4284 4337 5243 5840 6627 6895
Size: 72 399 220 53 906 597 787 268
Cuts at: 6895 1239
Size: 1524
Fragments arranged by size:
1524 906 820 787 597 459 399 393
307 268 220 145 106 72 62 62
53
EcoNI CCTnn'n_nnAGG
Cuts at: 2057 2057
Size: 7180
EcoO109I rG'GnC_Cy
Cuts at: 2376 2377 2610 3103 3359 3791 3934 4257 4284
Size: 1 233 493 256 432 143 323 27
Cuts at: 4284 4285 4457 2376
Size: 1 172 5099
Fragments arranged by size:
5099 493 432 323 256 233 172 143
27 1 1
EcoRI G'AATT_C
Cuts at: 4489 4867 4489
Size: 378 6802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 2362
Size: 7180
FspI TGC'GCA
Cuts at: 5686 5686
Size: 7180
HaeII r_GCGC'y
Cuts at: 1678 6327 1678
Size: 4649 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2137 2169 2858 2890 3421
Size: 32 135 32 565 32 689 32 531
Cuts at: 3421 3453 3994 4026 4244 4276 5821 5853 5895
Size: 32 541 32 218 32 1545 32 42
Cuts at: 5895 5927 1373
Size: 32 2626
Fragments arranged by size:
2626 1545 689 565 541 531 218 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2488 4524 4770 669
Size: 988 281 550 2036 246 3079
Fragments arranged by size:
3079 2036 988 550 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7180
HpaI GTT'AAC
Cuts at: 4770 4770
Size: 7180
KpnI G_GTAC'C
Cuts at: 4505 4505
Size: 7180
MfeI C'AATT_G
Cuts at: 2161 4779 2161
Size: 2618 4562
MluI A'CGCG_T
Cuts at: 7056 7056
Size: 7180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 2436 6294 6478 1305
Size: 831 300 3858 184 2007
Fragments arranged by size:
3858 2007 831 300 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3061 3563 4191 4206 4223
Size: 54 1425 343 1229 502 628 15 17
Cuts at: 4223 10
Size: 2967
Fragments arranged by size:
2967 1425 1229 628 502 343 54 17
15
MslI CAynn'nnrTG
Cuts at: 518 1166 2403 3074 4466 5138 5497 5656 518
Size: 648 1237 671 1392 672 359 159 2042
Fragments arranged by size:
2042 1392 1237 672 671 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2194 2329 3695 4596 5273 6421 6666 1606
Size: 588 135 1366 901 677 1148 245 2120
Fragments arranged by size:
2120 1366 1148 901 677 588 245 135
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5689
Fragments arranged by size:
5689 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7180
NheI G'CTAG_C
Cuts at: 1052 3875 1052
Size: 2823 4357
NotI GC'GGCC_GC
Cuts at: 4627 4627
Size: 7180
NspI r_CATG'y
Cuts at: 1132 1229 2366 3334 3746 4072 4538 6083 1132
Size: 97 1137 968 412 326 466 1545 2229
Fragments arranged by size:
2229 1545 1137 968 466 412 326 97
PasI CC'CwG_GG
Cuts at: 1348 2373 3336 3378 3929 1348
Size: 1025 963 42 551 4599
Fragments arranged by size:
4599 1025 963 551 42
PciI A'CATG_T
Cuts at: 6079 6079
Size: 7180
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 1108
Size: 902 6278
PfoI T'CCnGG_A
Cuts at: 2841 3189 3237 2841
Size: 348 48 6784
Fragments arranged by size:
6784 348 48
PmeI GTTT'AAAC
Cuts at: 4484 4484
Size: 7180
PmlI CAC'GTG
Cuts at: 2223 2223
Size: 7180
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2538 2570 3218 3250 4433 4465 5249 5281 6762
Size: 32 648 32 1183 32 784 32 1481
Cuts at: 6762 6794 2538
Size: 32 2924
Fragments arranged by size:
2924 1481 1183 784 648 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 3791 3934 4257 4457 3791
Size: 143 323 200 6514
Fragments arranged by size:
6514 323 200 143
PshAI GACnn'nnGTC
Cuts at: 1613 4214 1613
Size: 2601 4579
PsiI TTA'TAA
Cuts at: 4750 4750
Size: 7180
PspOMI G'GGCC_C
Cuts at: 2376 3359 4284 2376
Size: 983 925 5272
Fragments arranged by size:
5272 983 925
PstI C_TGCA'G
Cuts at: 830 2061 3700 4532 830
Size: 1231 1639 832 3478
Fragments arranged by size:
3478 1639 1231 832
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7180
PvuII CAG'CTG
Cuts at: 2194 2329 3695 2194
Size: 135 1366 5679
Fragments arranged by size:
5679 1366 135
SacI G_AGCT'C
Cuts at: 2178 4007 4418 2178
Size: 1829 411 4940
Fragments arranged by size:
4940 1829 411
SalI G'TCGA_C
Cuts at: 1936 4522 1936
Size: 2586 4594
SanDI GG'GwC_CC
Cuts at: 3791 3934 4457 3791
Size: 143 523 6514
Fragments arranged by size:
6514 523 143
SbfI CC_TGCA'GG
Cuts at: 4532 4532
Size: 7180
ScaI AGT'ACT
Cuts at: 5428 7140 5428
Size: 1712 5468
SfcI C'TryA_G
Cuts at: 826 1045 2057 2593 2677 3696 4528 5663 6344
Size: 219 1012 536 84 1019 832 1135 681
Cuts at: 6344 6535 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1019 1012 832 681 536 219
191 84
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7180
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7180
SmaI CCC'GGG
Cuts at: 1872 4507 1872
Size: 2635 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3823 4417 5284 6185 6447 820
Size: 197 926 1880 594 867 901 262 1553
Fragments arranged by size:
1880 1553 926 901 867 594 262 197
SnaBI TAC'GTA
Cuts at: 493 4971 493
Size: 4478 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7180
SphI G_CATG'C
Cuts at: 1132 4538 1132
Size: 3406 3774
SspI AAT'ATT
Cuts at: 5 52 5104 5
Size: 47 5052 2081
Fragments arranged by size:
5052 2081 47
StuI AGG'CCT
Cuts at: 1517 3818 1517
Size: 2301 4879
StyI C'CwwG_G
Cuts at: 513 1065 2004 3363 4619 513
Size: 552 939 1359 1256 3074
Fragments arranged by size:
3074 1359 1256 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3779 5207 5224 5377 5562 6007 325
Size: 1255 2199 1428 17 153 185 445 1498
Fragments arranged by size:
2199 1498 1428 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3739 5426 7138
Size: 274 80 33 51 1443 1762 1687 1712
Cuts at: 7138 96
Size: 138
Fragments arranged by size:
1762 1712 1687 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3142 3174 7140 7172 3142
Size: 32 3966 32 3150
Fragments arranged by size:
3966 3150 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2282 1316
Size: 966 6214
XbaI T'CTAG_A
Cuts at: 4516 4516
Size: 7180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3778 4199 4397 3778
Size: 421 198 6561
Fragments arranged by size:
6561 421 198
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7180
XmaI C'CCGG_G
Cuts at: 1870 4505 1870
Size: 2635 4545
XmnI GAAnn'nnTTC
Cuts at: 5309 5309
Size: 7180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4988 276
Size: 53 83 186 936 3454 2468
Fragments arranged by size:
3454 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI
BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SapI
SexAI SfiI SfoI SrfI SwaI