Restriction map for
pFN21AE1898
(Circular) (Six-base) MAPSORT of: pFN21AE1898.seq Check: 8272 from: 1 to: 7177
pFN21AE1898 7177 bp
With 182 enzymes: SgfI *
August 13, 2015 11:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3512 3512
Size: 7177
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4987 278
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2604 4520 1937
Size: 667 1916 4594
Fragments arranged by size:
4594 1916 667
Acc65I G'GTAC_C
Cuts at: 4498 4498
Size: 7177
AclI AA'CG_TT
Cuts at: 1563 2269 5304 5677 1563
Size: 706 3035 373 3063
Fragments arranged by size:
3063 3035 706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2532 2640 3708 5240 6624 6892 1632
Size: 900 108 1068 1532 1384 268 1917
Fragments arranged by size:
1917 1532 1384 1068 900 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6980
AflIII A'CryG_T
Cuts at: 1203 2197 2356 2673 2675 6076 7053 1203
Size: 994 159 317 2 3401 977 1327
Fragments arranged by size:
3401 1327 994 977 317 159 2
AgeI A'CCGG_T
Cuts at: 6008 6008
Size: 7177
AhdI GACnn_n'nnGTC
Cuts at: 5906 5906
Size: 7177
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7177
AlwNI CAG_nnn'CTG
Cuts at: 3519 3766 4920 6492 3519
Size: 247 1154 1572 4204
Fragments arranged by size:
4204 1572 1154 247
ApaLI G'TGCA_C
Cuts at: 5234 6390 5234
Size: 1156 6021
ApoI r'AATT_y
Cuts at: 1493 2281 4486 4712 4864 6973 1493
Size: 788 2205 226 152 2109 1697
Fragments arranged by size:
2205 2109 1697 788 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3618 5731 160
Size: 872 2586 2113 1606
Fragments arranged by size:
2586 2113 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
AvaI C'yCGr_G
Cuts at: 1870 1943 2074 2099 3445 4502 1870
Size: 73 131 25 1346 1057 4545
Fragments arranged by size:
4545 1346 1057 131 73 25
BamHI G'GATC_C
Cuts at: 4507 4874 4507
Size: 367 6810
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 4246 4498 5953 618
Size: 325 217 627 266 2193 252 1455 1842
Fragments arranged by size:
2193 1842 1455 627 325 266 252 217
BanII G_rGCy'C
Cuts at: 1391 4496 1391
Size: 3105 4072
BbeI G_GCGC'C
Cuts at: 2057 4250 2057
Size: 2193 4984
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4302 928
Size: 971 2403 3803
Fragments arranged by size:
3803 2403 971
BbvCI CC'TCA_GC
Cuts at: 2982 2982
Size: 7177
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5368 5402 1903
Size: 34 3431 34 3678
Fragments arranged by size:
3678 3431 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3321 5069 6279 3321
Size: 1748 1210 4219
Fragments arranged by size:
4219 1748 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7177
BfrBI ATG'CAT
Cuts at: 3738 3738
Size: 7177
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5788 136
Size: 107 122 71 631 605 4116 1525
Fragments arranged by size:
4116 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2179 7172 2179
Size: 4993 2184
BlpI GC'TnA_GC
Cuts at: 3980 4151 4594 3980
Size: 171 443 6563
Fragments arranged by size:
6563 443 171
Bme1580I G_kGCm'C
Cuts at: 1790 3277 3671 3843 5238 6394 1790
Size: 1487 394 172 1395 1156 2573
Fragments arranged by size:
2573 1487 1395 1156 394 172
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7177
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3113 4128 4908
Size: 416 53 83 399 24 1696 1015 780
Cuts at: 4908 5866 442
Size: 958 1753
Fragments arranged by size:
1753 1696 1015 958 780 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7177
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2031 2392 2031
Size: 361 6816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3660 3788 4194 5837 1239
Size: 2421 128 406 1643 2579
Fragments arranged by size:
2579 2421 1643 406 128
Bpu10I CC'TnA_GC
Cuts at: 2982 2982
Size: 7177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 5302 6167 6465 2843
Size: 2459 865 298 3555
Fragments arranged by size:
3555 2459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5840 882
Size: 617 4341 2219
Fragments arranged by size:
4341 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2359 2676 4968 493
Size: 711 1155 317 2292 2702
Fragments arranged by size:
2702 2292 1155 711 317
BsaBI GATnn'nnATC
Cuts at: 7171 7171
Size: 7177
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 4247 4984 5366
Size: 53 83 186 936 521 2193 737 382
Cuts at: 5366 6001 275
Size: 635 1451
Fragments arranged by size:
2193 1451 936 737 635 521 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2065 3253 4399 5610 6008 6282 6429 6840 2065
Size: 1188 1146 1211 398 274 147 411 2402
Fragments arranged by size:
2402 1211 1188 1146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6952
Fragments arranged by size:
6952 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7177
BseYI C'CCAG_C
Cuts at: 4301 6380 6876 4301
Size: 2079 496 4602
Fragments arranged by size:
4602 2079 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3505 3565 3631 1163
Size: 573 1769 60 66 4709
Fragments arranged by size:
4709 1769 573 66 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4627 5388 6416 1540
Size: 275 186 2626 761 1028 2301
Fragments arranged by size:
2626 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3164 5238 5323 6394 3164
Size: 2074 85 1071 3947
Fragments arranged by size:
3947 2074 1071 85
BsmI GAATG_Cn'
Cuts at: 2161 2853 4068 4688 4781 2161
Size: 692 1215 620 93 4557
Fragments arranged by size:
4557 1215 692 620 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3164 3277 3671 3843 4496 5238 5323
Size: 399 1374 113 394 172 653 742 85
Cuts at: 5323 6394 1391
Size: 1071 2174
Fragments arranged by size:
2174 1374 1071 742 653 399 394 172
113 85
BspEI T'CCGG_A
Cuts at: 2065 3253 6840 2065
Size: 1188 3587 2402
Fragments arranged by size:
3587 2402 1188
BspHI T'CATG_A
Cuts at: 3217 5066 6796 3217
Size: 1849 1730 3598
Fragments arranged by size:
3598 1849 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2804 3512 4532 844
Size: 1960 708 1020 3489
Fragments arranged by size:
3489 1960 1020 708
BsrBI CCG'CTC
Cuts at: 1394 1823 5064 1394
Size: 429 3241 3507
Fragments arranged by size:
3507 3241 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5672 5846 65
Size: 1183 4424 174 1396
Fragments arranged by size:
4424 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5821 6008 1700
Size: 110 4011 187 2869
Fragments arranged by size:
4011 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3893 96
Size: 3797 3380
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7177
BssSI C'ACGA_G
Cuts at: 3335 5237 6249 3335
Size: 1902 1012 4263
Fragments arranged by size:
4263 1902 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3272 3666 3810 794
Size: 2478 394 144 4161
Fragments arranged by size:
4161 2478 394 144
BstBI TT'CG_AA
Cuts at: 5981 5981
Size: 7177
BstEII G'GTnAC_C
Cuts at: 1184 4402 1184
Size: 3218 3959
BstYI r'GATC_y
Cuts at: 1972 2179 2659 3709 4384 4507 4874 5259 5276
Size: 207 480 1050 675 123 367 385 17
Cuts at: 5276 6728 6814 6826 7172 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1050 675 480 385 367 346
207 123 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3085 513
Size: 552 2020 4605
Fragments arranged by size:
4605 2020 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2687 487
Size: 683 56 1461 4977
Fragments arranged by size:
4977 1461 683 56
BtsI GCAGTG_nn'
Cuts at: 3553 3667 4693 5487 5507 3553
Size: 114 1026 794 20 5223
Fragments arranged by size:
5223 1026 794 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7142
DraI TTT'AAA
Cuts at: 2242 2565 2821 4432 4481 2242
Size: 323 256 1611 49 4938
Fragments arranged by size:
4938 1611 323 256 49
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4400 6184 809
Size: 507 3084 1784 1802
Fragments arranged by size:
3084 1802 1784 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3829 4624 5513
Size: 54 1425 50 275 18 1999 795 889
Cuts at: 5513 7142 8
Size: 1629 43
Fragments arranged by size:
1999 1629 1425 889 795 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4624 1537
Size: 275 2812 4090
Fragments arranged by size:
4090 2812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2574 2616 2641 2727 3459 5107 1353
Size: 1221 42 25 86 732 1648 3423
Fragments arranged by size:
3423 1648 1221 732 86 42 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5764 6138 6284 387
Size: 1064 138 4175 374 146 1280
Fragments arranged by size:
4175 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2532 2640 3660 3708 3788 4194 5240
Size: 393 900 108 1020 48 80 406 1046
Cuts at: 5240 5837 6624 6892 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1046 1020 900 787 597 406 393
268 108 80 48
EcoNI CCTnn'n_nnAGG
Cuts at: 3191 3191
Size: 7177
EcoO109I rG'GnC_Cy
Cuts at: 3820 4342 3820
Size: 522 6655
EcoRI G'AATT_C
Cuts at: 4486 4864 4486
Size: 378 6799
EcoRV GAT'ATC
Cuts at: 2518 2518
Size: 7177
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4363 4363
Size: 7177
FspI TGC'GCA
Cuts at: 5683 5683
Size: 7177
HaeII r_GCGC'y
Cuts at: 1678 2057 4250 6324 1678
Size: 379 2193 2074 2531
Fragments arranged by size:
2531 2193 2074 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2123 2155 2975 3007 3316
Size: 32 135 32 551 32 820 32 309
Cuts at: 3316 3348 4154 4186 5818 5850 5892 5924 1373
Size: 32 806 32 1632 32 42 32 2626
Fragments arranged by size:
2626 1632 820 806 551 309 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4456 4521 4767 669
Size: 988 281 2518 65 246 3079
Fragments arranged by size:
3079 2518 988 281 246 65
HindIII A'AGCT_T
Cuts at: 748 2105 748
Size: 1357 5820
HpaI GTT'AAC
Cuts at: 4767 4767
Size: 7177
KasI G'GCGC_C
Cuts at: 2053 4246 2053
Size: 2193 4984
KpnI G_GTAC'C
Cuts at: 4502 4502
Size: 7177
MfeI C'AATT_G
Cuts at: 4776 4776
Size: 7177
MluI A'CGCG_T
Cuts at: 7053 7053
Size: 7177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6291 6475 1305
Size: 4986 184 2007
Fragments arranged by size:
4986 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5355
Fragments arranged by size:
5355 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3090 3320 5135 5494 5653 518
Size: 648 1924 230 1815 359 159 2042
Fragments arranged by size:
2042 1924 1815 648 359 230 159
MspA1I CmG'CkG
Cuts at: 1606 3766 3834 3979 4367 4593 5270 6418 6663
Size: 2160 68 145 388 226 677 1148 245
Cuts at: 6663 1606
Size: 2120
Fragments arranged by size:
2160 2120 1148 677 388 245 226 145
68
NarI GG'CG_CC
Cuts at: 2054 4247 2054
Size: 2193 4984
NcoI C'CATG_G
Cuts at: 513 1065 3085 513
Size: 552 2020 4605
Fragments arranged by size:
4605 2020 552
NdeI CA'TA_TG
Cuts at: 387 4317 387
Size: 3930 3247
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7177
NotI GC'GGCC_GC
Cuts at: 4624 4624
Size: 7177
NsiI A_TGCA'T
Cuts at: 3740 3740
Size: 7177
NspI r_CATG'y
Cuts at: 1132 1229 2201 3783 4535 6080 1132
Size: 97 972 1582 752 1545 2229
Fragments arranged by size:
2229 1582 1545 972 752 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7177
PciI A'CATG_T
Cuts at: 2197 6076 2197
Size: 3879 3298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4084 4279 1108
Size: 2976 195 4006
Fragments arranged by size:
4006 2976 195
PfoI T'CCnGG_A
Cuts at: 3676 4290 3676
Size: 614 6563
PmeI GTTT'AAAC
Cuts at: 4481 4481
Size: 7177
PmlI CAC'GTG
Cuts at: 2359 2676 2359
Size: 317 6860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5246 5278 6759 6791 5246
Size: 32 1481 32 5632
Fragments arranged by size:
5632 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3820 4342 3820
Size: 522 6655
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7177
PsiI TTA'TAA
Cuts at: 2414 2505 4747 2414
Size: 91 2242 4844
Fragments arranged by size:
4844 2242 91
PspXI vC'TCGA_Gb
Cuts at: 3445 3445
Size: 7177
PstI C_TGCA'G
Cuts at: 830 3365 3818 4224 4440 4529 830
Size: 2535 453 406 216 89 3478
Fragments arranged by size:
3478 2535 453 406 216 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7177
PvuII CAG'CTG
Cuts at: 3766 3979 4367 3766
Size: 213 388 6576
Fragments arranged by size:
6576 388 213
SalI G'TCGA_C
Cuts at: 1936 4519 1936
Size: 2583 4594
SapI GCTCTTCn'nnn_
Cuts at: 2616 2641 2616
Size: 25 7152
SbfI CC_TGCA'GG
Cuts at: 4529 4529
Size: 7177
ScaI AGT'ACT
Cuts at: 2263 5425 7137 2263
Size: 3162 1712 2303
Fragments arranged by size:
3162 2303 1712
SexAI A'CCwGG_T
Cuts at: 2881 3204 2881
Size: 323 6854
SfcI C'TryA_G
Cuts at: 826 1045 2183 2710 3361 3814 4220 4436 4525
Size: 219 1138 527 651 453 406 216 89
Cuts at: 4525 5660 6341 6532 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1138 1135 681 651 527 453 406
219 216 191 89
SfoI GGC'GCC
Cuts at: 2055 4248 2055
Size: 2193 4984
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7177
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7177
SmaI CCC'GGG
Cuts at: 1872 2076 4504 1872
Size: 204 2428 4545
Fragments arranged by size:
4545 2428 204
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2099 2858 3445 5281 6182 6444
Size: 197 926 156 759 587 1836 901 262
Cuts at: 6444 820
Size: 1553
Fragments arranged by size:
1836 1553 926 901 759 587 262 197
156
SnaBI TAC'GTA
Cuts at: 493 4968 493
Size: 4475 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7177
SphI G_CATG'C
Cuts at: 1132 4535 1132
Size: 3403 3774
SspI AAT'ATT
Cuts at: 5 52 5101 5
Size: 47 5049 2081
Fragments arranged by size:
5049 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7177
StyI C'CwwG_G
Cuts at: 513 1065 3085 4616 513
Size: 552 2020 1531 3074
Fragments arranged by size:
3074 2020 1531 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5204 5221 5374 5559 6004 325
Size: 1255 3624 17 153 185 445 1498
Fragments arranged by size:
3624 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2261 2322 2738
Size: 274 80 33 51 1443 284 61 416
Cuts at: 2738 3893 3937 5423 7135 96
Size: 1155 44 1486 1712 138
Fragments arranged by size:
1712 1486 1443 1155 416 284 274 138
80 61 51 44 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4381 4413 7137 7169 4381
Size: 32 2724 32 4389
Fragments arranged by size:
4389 2724 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7177
XbaI T'CTAG_A
Cuts at: 4513 4513
Size: 7177
XhoI C'TCGA_G
Cuts at: 1943 2099 3445 1943
Size: 156 1346 5675
Fragments arranged by size:
5675 1346 156
XmaI C'CCGG_G
Cuts at: 1870 2074 4502 1870
Size: 204 2428 4545
Fragments arranged by size:
4545 2428 204
XmnI GAAnn'nnTTC
Cuts at: 5306 5306
Size: 7177
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4985 276
Size: 53 83 186 936 3451 2468
Fragments arranged by size:
3451 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI NaeI
NgoMIV NruI PacI PspOMI PsrI RsrII SacI SacII
SanDI SfiI SrfI SwaI XcmI