Restriction map for
pFN21AE1893
(Circular) (Six-base) MAPSORT of: pFN21AE1893.seq Check: 3559 from: 1 to: 5971
pFN21AE1893 5971 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 2430 3292 2430
Size: 862 5109
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2234 2777 4034 5418 5686 1632
Size: 602 543 1257 1384 268 1917
Fragments arranged by size:
1917 1384 1257 602 543 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5774
AflIII A'CryG_T
Cuts at: 1203 2763 4870 5847 1203
Size: 1560 2107 977 1327
Fragments arranged by size:
2107 1560 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 2641 4700 2641
Size: 2059 3912
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5971
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2798 2884 1184
Size: 1614 86 4271
Fragments arranged by size:
4271 1614 86
AlwNI CAG_nnn'CTG
Cuts at: 2464 3007 3714 5286 2464
Size: 543 707 1572 3149
Fragments arranged by size:
3149 1572 707 543
ApaLI G'TGCA_C
Cuts at: 4028 5184 4028
Size: 1156 4815
ApoI r'AATT_y
Cuts at: 1493 2236 3122 3280 3506 3658 5767 1493
Size: 743 886 158 226 152 2109 1697
Fragments arranged by size:
2109 1697 886 743 226 158 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 2562 3296 1870
Size: 73 619 734 4545
Fragments arranged by size:
4545 734 619 73
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2430 3292 4747 618
Size: 325 217 627 643 862 1455 1842
Fragments arranged by size:
1842 1455 862 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2041 2943 3290 1391
Size: 650 902 347 4072
Fragments arranged by size:
4072 902 650 347
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5971
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4582 136
Size: 107 122 71 631 605 337 2573 1525
Fragments arranged by size:
2573 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 4032 5188 1790
Size: 2242 1156 2573
Fragments arranged by size:
2573 2242 1156
BmgBI CAC'GTC
Cuts at: 1318 3085 1318
Size: 1767 4204
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2035 2171 3173
Size: 416 53 83 399 24 618 136 1002
Cuts at: 3173 3702 4660 442
Size: 529 958 1753
Fragments arranged by size:
1753 1002 958 618 529 416 399 136
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2886 1056
Size: 1830 4141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4631 1239
Size: 3392 2579
Bpu10I CC'TnA_GC
Cuts at: 2543 2877 2543
Size: 334 5637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4634 882
Size: 617 3135 2219
Fragments arranged by size:
3135 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3762 493
Size: 711 2558 2702
Fragments arranged by size:
2702 2558 711
BsaBI GATnn'nnATC
Cuts at: 5965 5965
Size: 5971
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3778 4160 4795 275
Size: 53 83 186 936 2245 382 635 1451
Fragments arranged by size:
2245 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5746
Fragments arranged by size:
5746 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5971
BseYI C'CCAG_C
Cuts at: 5174 5670 5174
Size: 496 5475
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5398
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2062 3421 4182 5210 1540
Size: 275 186 61 1359 761 1028 2301
Fragments arranged by size:
2301 1359 1028 761 275 186 61
BsiHKAI G_wGCw'C
Cuts at: 2943 4032 4117 5188 2943
Size: 1089 85 1071 3726
Fragments arranged by size:
3726 1089 1071 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2943 3290 4032 4117 5188 1391
Size: 399 251 902 347 742 85 1071 2174
Fragments arranged by size:
2174 1071 902 742 399 347 251 85
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2559 3326 844
Size: 1715 767 3489
Fragments arranged by size:
3489 1715 767
BsrBI CCG'CTC
Cuts at: 1394 1823 2820 3858 1394
Size: 429 997 1038 3507
Fragments arranged by size:
3507 1038 997 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2441 4466 4640 65
Size: 1183 1193 2025 174 1396
Fragments arranged by size:
2025 1396 1193 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4615 4802 1700
Size: 110 2805 187 2869
Fragments arranged by size:
2869 2805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5971
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 4775 4775
Size: 5971
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2165 3301 3668 4053 4070 5522 5608 5620
Size: 193 1136 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1136 385 367 346 193 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5232
Fragments arranged by size:
5232 683 56
BtsI GCAGTG_nn'
Cuts at: 2136 3487 4281 4301 2136
Size: 1351 794 20 3806
Fragments arranged by size:
3806 1351 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 3275 3275
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2973 3418 4307
Size: 54 1425 50 275 18 1143 445 889
Cuts at: 4307 5936 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1143 889 445 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2283 2661 3901 1353
Size: 765 165 378 1240 3423
Fragments arranged by size:
3423 1240 765 378 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2941 2941
Size: 5971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2234 2777 4034 4631 5418 5686 1239
Size: 393 602 543 1257 597 787 268 1524
Fragments arranged by size:
1524 1257 787 602 597 543 393 268
EcoRI G'AATT_C
Cuts at: 2236 3280 3658 2236
Size: 1044 378 4549
Fragments arranged by size:
4549 1044 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2531 2531
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 4612 4644 4686
Size: 32 135 32 1055 32 1953 32 42
Cuts at: 4686 4718 1373
Size: 32 2626
Fragments arranged by size:
2626 1953 1055 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2385 2825 748
Size: 1637 440 3894
Fragments arranged by size:
3894 1637 440
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 2434 3296 2434
Size: 862 5109
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5085 5269 1305
Size: 3780 184 2007
Fragments arranged by size:
3780 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2975 10
Size: 54 1425 343 1143 3006
Fragments arranged by size:
3006 1425 1143 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2866 3929 4288 4447 518
Size: 648 1700 1063 359 159 2042
Fragments arranged by size:
2042 1700 1063 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2461 3387 4064 5212 5457 1606
Size: 855 926 677 1148 245 2120
Fragments arranged by size:
2120 1148 926 855 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4480
Fragments arranged by size:
4480 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 2882 1052
Size: 1830 4141
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 2641 2767 3329 4874 1132
Size: 97 1412 126 562 1545 2229
Fragments arranged by size:
2229 1545 1412 562 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 2763 4870 2763
Size: 2107 3864
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 2812 1108
Size: 1281 423 4267
Fragments arranged by size:
4267 1281 423
PfoI T'CCnGG_A
Cuts at: 2154 2943 2154
Size: 789 5182
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4040 4072 5553 5585 4040
Size: 32 1481 32 4426
Fragments arranged by size:
4426 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2404 1613
Size: 791 5180
PsiI TTA'TAA
Cuts at: 3541 3541
Size: 5971
PstI C_TGCA'G
Cuts at: 830 2197 3323 830
Size: 1367 1126 3478
Fragments arranged by size:
3478 1367 1126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2461 2461
Size: 5971
SacI G_AGCT'C
Cuts at: 2943 2943
Size: 5971
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 4219 5931 4219
Size: 1712 4259
SfcI C'TryA_G
Cuts at: 826 1045 2193 2310 3319 4454 5135 5326 826
Size: 219 1148 117 1009 1135 681 191 1471
Fragments arranged by size:
1471 1148 1135 1009 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4075 4976 5238 820
Size: 197 926 2132 901 262 1553
Fragments arranged by size:
2132 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 3895 5
Size: 47 3843 2081
Fragments arranged by size:
3843 2081 47
StuI AGG'CCT
Cuts at: 1517 2069 1517
Size: 552 5419
StyI C'CwwG_G
Cuts at: 513 1065 2004 2631 2739 3075 3410 513
Size: 552 939 627 108 336 335 3074
Fragments arranged by size:
3074 939 627 552 336 335 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3998 4015 4168 4353 4798 325
Size: 1255 2418 17 153 185 445 1498
Fragments arranged by size:
2418 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2412 2509 2760
Size: 274 80 33 51 1443 435 97 251
Cuts at: 2760 3037 4217 5929 96
Size: 277 1180 1712 138
Fragments arranged by size:
1712 1443 1180 435 277 274 251 138
97 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3082 3082
Size: 5971
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 3213 4100 3213
Size: 887 5084
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI