Restriction map for
pFN21AE1891
(Circular) (Six-base) MAPSORT of: pFN21AE1891.seq Check: 6141 from: 1 to: 5965
pFN21AE1891 5965 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2044 2044
Size: 5965
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3775 278
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3308 1937
Size: 1371 4594
Acc65I G'GTAC_C
Cuts at: 3286 3286
Size: 5965
AclI AA'CG_TT
Cuts at: 1563 4092 4465 1563
Size: 2529 373 3063
Fragments arranged by size:
3063 2529 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 4028 5412 5680 1632
Size: 968 1428 1384 268 1917
Fragments arranged by size:
1917 1428 1384 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5768
AflIII A'CryG_T
Cuts at: 1203 4864 5841 1203
Size: 3661 977 1327
Fragments arranged by size:
3661 1327 977
AgeI A'CCGG_T
Cuts at: 4796 4796
Size: 5965
AhdI GACnn_n'nnGTC
Cuts at: 4694 4694
Size: 5965
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5965
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3152 1184
Size: 1968 3997
AlwNI CAG_nnn'CTG
Cuts at: 2262 3708 5280 2262
Size: 1446 1572 2947
Fragments arranged by size:
2947 1572 1446
ApaLI G'TGCA_C
Cuts at: 4022 5178 4022
Size: 1156 4809
ApoI r'AATT_y
Cuts at: 1493 2510 3274 3500 3652 5761 1493
Size: 1017 764 226 152 2109 1697
Fragments arranged by size:
2109 1697 1017 764 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4519 160
Size: 872 3487 1606
Fragments arranged by size:
3487 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
AvaI C'yCGr_G
Cuts at: 1870 1943 2231 2300 2850 3290 1870
Size: 73 288 69 550 440 4545
Fragments arranged by size:
4545 550 440 288 73 69
BamHI G'GATC_C
Cuts at: 3295 3662 3295
Size: 367 5598
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2185 3049 3286 4741 618
Size: 325 217 627 398 864 237 1455 1842
Fragments arranged by size:
1842 1455 864 627 398 325 237 217
BanII G_rGCy'C
Cuts at: 1391 2114 2754 3284 1391
Size: 723 640 530 4072
Fragments arranged by size:
4072 723 640 530
BbeI G_GCGC'C
Cuts at: 3053 3053
Size: 5965
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4156 4190 1903
Size: 34 2219 34 3678
Fragments arranged by size:
3678 2219 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3857 5067 3857
Size: 1210 4755
BclI T'GATC_A
Cuts at: 1548 2932 1548
Size: 1384 4581
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3185 4576
Size: 107 122 71 631 605 337 1176 1391
Cuts at: 4576 136
Size: 1525
Fragments arranged by size:
1525 1391 1176 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2687 5960 2687
Size: 3273 2692
BlpI GC'TnA_GC
Cuts at: 2315 3382 2315
Size: 1067 4898
Bme1580I G_kGCm'C
Cuts at: 1790 2206 2829 4026 5182 1790
Size: 416 623 1197 1156 2573
Fragments arranged by size:
2573 1197 1156 623 416
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5965
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2108 3696 4654
Size: 416 53 83 399 24 691 1588 958
Cuts at: 4654 442
Size: 1753
Fragments arranged by size:
1753 1588 958 691 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2436 3116 4625 1239
Size: 1197 680 1509 2579
Fragments arranged by size:
2579 1509 1197 680
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2393 3262 4090 4955 5253 2393
Size: 869 828 865 298 3105
Fragments arranged by size:
3105 869 865 828 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2014 3071 4628 882
Size: 617 515 1057 1557 2219
Fragments arranged by size:
2219 1557 1057 617 515
BsaAI yAC'GTr
Cuts at: 493 1204 3756 493
Size: 711 2552 2702
Fragments arranged by size:
2702 2552 711
BsaBI GATnn'nnATC
Cuts at: 5959 5959
Size: 5965
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3050 3772 4154 4789
Size: 53 83 186 936 1517 722 382 635
Cuts at: 4789 275
Size: 1451
Fragments arranged by size:
1517 1451 936 722 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2135 2787 2984 4398 4796 5070 5217 5628 2135
Size: 652 197 1414 398 274 147 411 2472
Fragments arranged by size:
2472 1414 652 411 398 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2130 2160 1178
Size: 30 165 30 727 30 4983
Fragments arranged by size:
4983 727 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 1186
Size: 1063 4902
BseYI C'CCAG_C
Cuts at: 2088 2255 2696 2922 5168 5664 2088
Size: 167 441 226 2246 496 2389
Fragments arranged by size:
2389 2246 496 441 226 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5392
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2045 3415 4176 5204 1540
Size: 275 186 44 1370 761 1028 2301
Fragments arranged by size:
2301 1370 1028 761 275 186 44
BsiHKAI G_wGCw'C
Cuts at: 2763 3122 4026 4111 5182 2763
Size: 359 904 85 1071 3546
Fragments arranged by size:
3546 1071 904 359 85
BsmI GAATG_Cn'
Cuts at: 2620 3476 3569 2620
Size: 856 93 5016
Fragments arranged by size:
5016 856 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2114 2206 2754 2763 2829 3122 3284
Size: 399 324 92 548 9 66 293 162
Cuts at: 3284 4026 4111 5182 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 548 399 324 293 162
92 85 66 9
BspEI T'CCGG_A
Cuts at: 2135 2787 5628 2135
Size: 652 2841 2472
Fragments arranged by size:
2841 2472 652
BspHI T'CATG_A
Cuts at: 3854 5584 3854
Size: 1730 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2044 2844 3320 844
Size: 1200 800 476 3489
Fragments arranged by size:
3489 1200 800 476
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 3852 1394
Size: 429 407 1622 3507
Fragments arranged by size:
3507 1622 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 4460 4634 65
Size: 1183 3212 174 1396
Fragments arranged by size:
3212 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3052 4609 4796 1700
Size: 110 1242 1557 187 2869
Fragments arranged by size:
2869 1557 1242 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5965
BssHII G'CGCG_C
Cuts at: 1925 2158 1925
Size: 233 5732
BssSI C'ACGA_G
Cuts at: 4025 5037 4025
Size: 1012 4953
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2626 794
Size: 1832 4133
BstBI TT'CG_AA
Cuts at: 3204 4769 3204
Size: 1565 4400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5965
BstYI r'GATC_y
Cuts at: 1972 2370 2687 3295 3662 4047 4064 5516 5602
Size: 398 317 608 367 385 17 1452 86
Cuts at: 5602 5614 5960 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 608 398 385 367 346 317
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2917 3180 513
Size: 552 939 913 263 3298
Fragments arranged by size:
3298 939 913 552 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5226
Fragments arranged by size:
5226 683 56
BtsI GCAGTG_nn'
Cuts at: 2326 2624 3481 4275 4295 2326
Size: 298 857 794 20 3996
Fragments arranged by size:
3996 857 794 298 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5965
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5930
DraI TTT'AAA
Cuts at: 3269 3269
Size: 5965
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2852 4972 809
Size: 507 1536 2120 1802
Fragments arranged by size:
2120 1802 1536 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3111 3177 3412
Size: 54 1425 50 275 18 1281 66 235
Cuts at: 3412 4301 5930 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1281 889 275 235 66 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3412 1537
Size: 275 1600 4090
Fragments arranged by size:
4090 1600 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2262 2961 3895 1353
Size: 909 699 934 3423
Fragments arranged by size:
3423 934 909 699
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2122 3248 4552 4926 5072 387
Size: 1064 138 533 1126 1304 374 146 1280
Fragments arranged by size:
1304 1280 1126 1064 533 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2436 2600 3116 4028 4625 5412 5680
Size: 393 804 164 516 912 597 787 268
Cuts at: 5680 1239
Size: 1524
Fragments arranged by size:
1524 912 804 787 597 516 393 268
164
EcoNI CCTnn'n_nnAGG
Cuts at: 2501 2501
Size: 5965
EcoO109I rG'GnC_Cy
Cuts at: 2071 2071
Size: 5965
EcoRI G'AATT_C
Cuts at: 3274 3652 3274
Size: 378 5587
EcoRV GAT'ATC
Cuts at: 2521 2521
Size: 5965
FspI TGC'GCA
Cuts at: 4471 4471
Size: 5965
HaeII r_GCGC'y
Cuts at: 1678 2015 2089 2102 3053 5112 1678
Size: 337 74 13 951 2059 2531
Fragments arranged by size:
2531 2059 951 337 74 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2584 2616 4606
Size: 32 135 32 656 32 324 32 1990
Cuts at: 4606 4638 4680 4712 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1990 656 324 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3309 3555 669
Size: 988 281 1371 246 3079
Fragments arranged by size:
3079 1371 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2292 748
Size: 1544 4421
HpaI GTT'AAC
Cuts at: 3555 3555
Size: 5965
KasI G'GCGC_C
Cuts at: 3049 3049
Size: 5965
KpnI G_GTAC'C
Cuts at: 3290 3290
Size: 5965
MfeI C'AATT_G
Cuts at: 3564 3564
Size: 5965
MluI A'CGCG_T
Cuts at: 5841 5841
Size: 5965
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2559 5079 5263 1305
Size: 1254 2520 184 2007
Fragments arranged by size:
2520 2007 1254 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4143
Fragments arranged by size:
4143 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 2958 3923 4282 4441 518
Size: 648 1338 454 965 359 159 2042
Fragments arranged by size:
2042 1338 965 648 454 359 159
MspA1I CmG'CkG
Cuts at: 1606 2696 3059 3109 3220 3381 4058 5206 5451
Size: 1090 363 50 111 161 677 1148 245
Cuts at: 5451 1606
Size: 2120
Fragments arranged by size:
2120 1148 1090 677 363 245 161 111
50
NaeI GCC'GGC
Cuts at: 3054 3054
Size: 5965
NarI GG'CG_CC
Cuts at: 3050 3050
Size: 5965
NcoI C'CATG_G
Cuts at: 513 1065 2004 2917 3180 513
Size: 552 939 913 263 3298
Fragments arranged by size:
3298 939 913 552 263
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5965
NgoMIV G'CCGG_C
Cuts at: 3052 3052
Size: 5965
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5965
NotI GC'GGCC_GC
Cuts at: 3412 3412
Size: 5965
NspI r_CATG'y
Cuts at: 1132 1229 3323 4868 1132
Size: 97 2094 1545 2229
Fragments arranged by size:
2229 2094 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2663 1348
Size: 1315 4650
PciI A'CATG_T
Cuts at: 4864 4864
Size: 5965
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2262 1108
Size: 1154 4811
PmeI GTTT'AAAC
Cuts at: 3269 3269
Size: 5965
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4034 4066 5547 5579 4034
Size: 32 1481 32 4420
Fragments arranged by size:
4420 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2071
Size: 5965
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5965
PsiI TTA'TAA
Cuts at: 3535 3535
Size: 5965
PspXI vC'TCGA_Gb
Cuts at: 2231 2231
Size: 5965
PstI C_TGCA'G
Cuts at: 830 2055 2695 2711 3108 3317 830
Size: 1225 640 16 397 209 3478
Fragments arranged by size:
3478 1225 640 397 209 16
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5965
PvuII CAG'CTG
Cuts at: 2696 3059 3109 3220 2696
Size: 363 50 111 5441
Fragments arranged by size:
5441 363 111 50
SalI G'TCGA_C
Cuts at: 1936 3307 1936
Size: 1371 4594
SbfI CC_TGCA'GG
Cuts at: 3317 3317
Size: 5965
ScaI AGT'ACT
Cuts at: 4213 5925 4213
Size: 1712 4253
SfcI C'TryA_G
Cuts at: 826 1045 2051 2691 2707 3104 3313 4448 5129
Size: 219 1006 640 16 397 209 1135 681
Cuts at: 5129 5320 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1006 681 640 397 219 209
191 16
SfoI GGC'GCC
Cuts at: 3051 3051
Size: 5965
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5965
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5965
SmaI CCC'GGG
Cuts at: 1872 2302 3292 1872
Size: 430 990 4545
Fragments arranged by size:
4545 990 430
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 2408 3241 4069 4970 5232
Size: 197 926 288 177 833 828 901 262
Cuts at: 5232 820
Size: 1553
Fragments arranged by size:
1553 926 901 833 828 288 262 197
177
SnaBI TAC'GTA
Cuts at: 493 3756 493
Size: 3263 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5965
SphI G_CATG'C
Cuts at: 1132 3323 1132
Size: 2191 3774
SspI AAT'ATT
Cuts at: 5 52 3889 5
Size: 47 3837 2081
Fragments arranged by size:
3837 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5965
StyI C'CwwG_G
Cuts at: 513 1065 2004 2917 3180 3404 513
Size: 552 939 913 263 224 3074
Fragments arranged by size:
3074 939 913 552 263 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2914 3992 4009 4162 4347 4792 325
Size: 1255 1334 1078 17 153 185 445 1498
Fragments arranged by size:
1498 1334 1255 1078 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4211 5923 96
Size: 274 80 33 51 1443 2234 1712 138
Fragments arranged by size:
2234 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 5925 5957 2130
Size: 32 3763 32 2138
Fragments arranged by size:
3763 2138 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5965
XbaI T'CTAG_A
Cuts at: 2449 3301 2449
Size: 852 5113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2734 2011
Size: 723 5242
XhoI C'TCGA_G
Cuts at: 1943 2231 1943
Size: 288 5677
XmaI C'CCGG_G
Cuts at: 1870 2300 3290 1870
Size: 430 990 4545
Fragments arranged by size:
4545 990 430
XmnI GAAnn'nnTTC
Cuts at: 4094 4094
Size: 5965
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3773 276
Size: 53 83 186 936 2239 2468
Fragments arranged by size:
2468 2239 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI