Restriction map for
pFN21AE1890
(Circular) (Six-base) MAPSORT of: pFN21AE1890.seq Check: 2513 from: 1 to: 5959
pFN21AE1890 5959 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3216 3216
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2244 3302 1937
Size: 307 1058 4594
Fragments arranged by size:
4594 1058 307
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 2264 4086 4459 1563
Size: 701 1822 373 3063
Fragments arranged by size:
3063 1822 701 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AfeI AGC'GCT
Cuts at: 2921 2921
Size: 5959
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2946 4858 5835 1203
Size: 1743 1912 977 1327
Fragments arranged by size:
1912 1743 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 3231 3702 5274 3231
Size: 471 1572 3916
Fragments arranged by size:
3916 1572 471
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2328 2661 2883 3074 3284 1870
Size: 73 385 333 222 191 210 4545
Fragments arranged by size:
4545 385 333 222 210 191 73
AvrII C'CTAG_G
Cuts at: 2830 2830
Size: 5959
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2370 928
Size: 971 471 4517
Fragments arranged by size:
4517 971 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2474 2508 4150 4184 1903
Size: 34 537 34 1642 34 3678
Fragments arranged by size:
3678 1642 537 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2865 1548
Size: 1317 4642
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2856 4570 136
Size: 107 122 71 631 605 1184 1714 1525
Fragments arranged by size:
1714 1525 1184 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2670 5954 2670
Size: 3284 2675
BlpI GC'TnA_GC
Cuts at: 2415 3376 2415
Size: 961 4998
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2065 4619 1239
Size: 826 2554 2579
Fragments arranged by size:
2579 2554 826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2758 2809 3750 493
Size: 711 1554 51 941 2702
Fragments arranged by size:
2702 1554 941 711 51
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2526 3132 4392 4790 5064 5211 5622 2526
Size: 606 1260 398 274 147 411 2863
Fragments arranged by size:
2863 1260 606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2732 2762 2798 2828 1178
Size: 30 165 30 1329 30 36 30 4309
Fragments arranged by size:
4309 1329 165 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2494 2654 2740 2806 2975 1186
Size: 1308 160 86 66 169 4170
Fragments arranged by size:
4170 1308 169 160 86 66
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3244 1163
Size: 573 1508 3878
Fragments arranged by size:
3878 1508 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3255 3409 4170 5198 1540
Size: 275 186 1254 154 761 1028 2301
Fragments arranged by size:
2301 1254 1028 761 275 186 154
BsiHKAI G_wGCw'C
Cuts at: 2526 4020 4105 5176 2526
Size: 1494 85 1071 3309
Fragments arranged by size:
3309 1494 1071 85
BsmI GAATG_Cn'
Cuts at: 2225 3470 3563 2225
Size: 1245 93 4621
Fragments arranged by size:
4621 1245 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2526 3278 4020 4105 5176 1391
Size: 399 736 752 742 85 1071 2174
Fragments arranged by size:
2174 1071 752 742 736 399 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3195 3216 3314 844
Size: 2351 21 98 3489
Fragments arranged by size:
3489 2351 98 21
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2164 4454 4628 65
Size: 1183 916 2290 174 1396
Fragments arranged by size:
2290 1396 1183 916 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 4603 4790 1700
Size: 110 887 1906 187 2869
Fragments arranged by size:
2869 1906 887 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 2957 3100 4019 5031 2957
Size: 143 919 1012 3885
Fragments arranged by size:
3885 1012 919 143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3036 794
Size: 2242 3717
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2670 3289 3656 4041 4058 5510 5596 5608
Size: 698 619 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 698 619 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2182 2851 3255 513
Size: 552 1117 669 404 3217
Fragments arranged by size:
3217 1117 669 552 404
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2439 487
Size: 683 56 1213 4007
Fragments arranged by size:
4007 1213 683 56
BtsI GCAGTG_nn'
Cuts at: 2977 3208 3475 4269 4289 2977
Size: 231 267 794 20 4647
Fragments arranged by size:
4647 794 267 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3252 3406 4295
Size: 54 1425 50 275 18 1422 154 889
Cuts at: 4295 5924 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1422 889 275 154 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3252 3406 1537
Size: 275 1440 154 4090
Fragments arranged by size:
4090 1440 275 154
EarI CTCTTCn'nnn_
Cuts at: 1353 2471 3889 1353
Size: 1118 1418 3423
Fragments arranged by size:
3423 1418 1118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2917 3005 4546 4920 5066 387
Size: 1064 138 1328 88 1541 374 146 1280
Fragments arranged by size:
1541 1328 1280 1064 374 146 138 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2065 4022 4619 5406 5674 1239
Size: 393 433 1957 597 787 268 1524
Fragments arranged by size:
1957 1524 787 597 433 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2338 2834 2338
Size: 496 5463
EcoO109I rG'GnC_Cy
Cuts at: 2604 2779 2839 2604
Size: 175 60 5724
Fragments arranged by size:
5724 175 60
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2435 2435
Size: 5959
FspI TGC'GCA
Cuts at: 2651 4465 2651
Size: 1814 4145
FspAI rTGC'GCAy
Cuts at: 2651 2651
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2923 5106 1678
Size: 1245 2183 2531
Fragments arranged by size:
2531 2183 1245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2100 2132 2732 2764 3083
Size: 32 135 32 528 32 600 32 319
Cuts at: 3083 3115 4600 4632 4674 4706 1373
Size: 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 600 528 319 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2285 3048 5073 5257 1305
Size: 980 763 2025 184 2007
Fragments arranged by size:
2025 2007 980 763 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3917 4276 4435 518
Size: 648 2751 359 159 2042
Fragments arranged by size:
2751 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2853 3257 3375 4052 5200 5445 1606
Size: 479 768 404 118 677 1148 245 2120
Fragments arranged by size:
2120 1148 768 677 479 404 245 118
NcoI C'CATG_G
Cuts at: 513 1065 2182 513
Size: 552 1117 4290
Fragments arranged by size:
4290 1117 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3252 3406 3252
Size: 154 5805
NspI r_CATG'y
Cuts at: 1132 1229 2950 3034 3052 3317 4862 1132
Size: 97 1721 84 18 265 1545 2229
Fragments arranged by size:
2229 1721 1545 265 97 84 18
PasI CC'CwG_GG
Cuts at: 1348 2542 1348
Size: 1194 4765
PciI A'CATG_T
Cuts at: 2946 4858 2946
Size: 1912 4047
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PfoI T'CCnGG_A
Cuts at: 2625 2625
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2779 2839 2779
Size: 60 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2942 3311 830
Size: 2112 369 3478
Fragments arranged by size:
3478 2112 369
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacII CC_GC'GG
Cuts at: 2854 3258 2854
Size: 404 5555
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2448 2938 3307 4442 5123 5314 826
Size: 219 1403 490 369 1135 681 191 1471
Fragments arranged by size:
1471 1403 1135 681 490 369 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2663 2885 3286 1872
Size: 791 222 401 4545
Fragments arranged by size:
4545 791 401 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3052 3317 1132
Size: 1920 265 3774
Fragments arranged by size:
3774 1920 265
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2182 2600 2769 2830 3398 513
Size: 552 1117 418 169 61 568 3074
Fragments arranged by size:
3074 1117 568 552 418 169 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2037 3986 4003 4156 4341 4786
Size: 1255 444 13 1949 17 153 185 445
Cuts at: 4786 325
Size: 1498
Fragments arranged by size:
1949 1498 1255 445 444 185 153 17
13
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2934 4205 5917
Size: 274 80 33 51 1443 957 1271 1712
Cuts at: 5917 96
Size: 138
Fragments arranged by size:
1712 1443 1271 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 2240 1316
Size: 924 5035
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2661 2883 3284 1870
Size: 791 222 401 4545
Fragments arranged by size:
4545 791 401 222
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI